Restriction map for
pFN21AB5456
(Circular) (Six-base) MAPSORT of: pFN21AB5456.seq Check: 2745 from: 1 to: 5962
pFN21AB5456 5962 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2133 2587 2686 2133
Size: 454 99 5409
Fragments arranged by size:
5409 454 99
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2447 3772 278
Size: 53 83 186 936 911 1325 2468
Fragments arranged by size:
2468 1325 936 911 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 1937
Size: 1368 4594
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2115 2366 2408 2876 2927 3053 4025 5409
Size: 483 251 42 468 51 126 972 1384
Cuts at: 5409 5677 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 972 483 468 268 251 126
51 42
AfeI AGC'GCT
Cuts at: 2162 2228 2162
Size: 66 5896
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 3241 4861 5838 1203
Size: 2038 1620 977 1327
Fragments arranged by size:
2038 1620 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2261 2293 2261
Size: 32 5930
AlwNI CAG_nnn'CTG
Cuts at: 2218 2368 2512 3705 5277 2218
Size: 150 144 1193 1572 2903
Fragments arranged by size:
2903 1572 1193 150 144
ApaI G_GGCC'C
Cuts at: 2527 2527
Size: 5962
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 3271 3497 3649 5758 1493
Size: 1778 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2453 2526 2958 3170 3287 1870
Size: 73 510 73 432 212 117 4545
Fragments arranged by size:
4545 510 432 212 117 73 73
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2013 3283 4738 618
Size: 325 217 627 226 1270 1455 1842
Fragments arranged by size:
1842 1455 1270 627 325 226 217
BanII G_rGCy'C
Cuts at: 1391 2187 2527 2988 3167 3281 1391
Size: 796 340 461 179 114 4072
Fragments arranged by size:
4072 796 461 340 179 114
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4991
BbvCI CC'TCA_GC
Cuts at: 2412 2412
Size: 5962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3854 5064 3854
Size: 1210 4752
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2531 2819 2828
Size: 107 122 71 631 605 859 288 9
Cuts at: 2828 4573 136
Size: 1745 1525
Fragments arranged by size:
1745 1525 859 631 605 288 122 107
71 9
BglII A'GATC_T
Cuts at: 2788 5957 2788
Size: 3169 2793
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 2527 4023 5179 1790
Size: 737 1496 1156 2573
Fragments arranged by size:
2573 1496 1156 737
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2046 2093 2683
Size: 416 53 83 399 24 629 47 590
Cuts at: 2683 3693 4651 442
Size: 1010 958 1753
Fragments arranged by size:
1753 1010 958 629 590 416 399 83
53 47 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2189 2351 2534 2576 2789 3023 4622
Size: 902 48 162 183 42 213 234 1599
Cuts at: 4622 1239
Size: 2579
Fragments arranged by size:
2579 1599 902 234 213 183 162 48
42
Bpu10I CC'TnA_GC
Cuts at: 2412 2412
Size: 5962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4087 4952 5250 4087
Size: 865 298 4799
Fragments arranged by size:
4799 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2144 2885 3097 4625 882
Size: 617 645 741 212 1528 2219
Fragments arranged by size:
2219 1528 741 645 617 212
BsaAI yAC'GTr
Cuts at: 493 1204 3753 493
Size: 711 2549 2702
Fragments arranged by size:
2702 2549 711
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2444 3769 4151 4786
Size: 53 83 186 936 911 1325 382 635
Cuts at: 4786 275
Size: 1451
Fragments arranged by size:
1451 1325 936 911 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2899 4395 4793 5067 5214 5625 2899
Size: 1496 398 274 147 411 3236
Fragments arranged by size:
3236 1496 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2261 2291 2787 2817 1178
Size: 30 165 30 858 30 496 30 4323
Fragments arranged by size:
4323 858 496 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2088 2186 2249 2282 2684 2831 2876 3121
Size: 902 98 63 33 402 147 45 245
Cuts at: 3121 3172 3175 3188 1186
Size: 51 3 13 3960
Fragments arranged by size:
3960 902 402 245 147 98 63 51
45 33 13 3
BseYI C'CCAG_C
Cuts at: 3149 5165 5661 3149
Size: 2016 496 3450
Fragments arranged by size:
3450 2016 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2066 2105 2615 1163
Size: 573 330 39 510 4510
Fragments arranged by size:
4510 573 510 330 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3412 4173 5201 1540
Size: 275 186 1411 761 1028 2301
Fragments arranged by size:
2301 1411 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2187 2385 2988 4023 4108 5179 2187
Size: 198 603 1035 85 1071 2970
Fragments arranged by size:
2970 1071 1035 603 198 85
BsmI GAATG_Cn'
Cuts at: 2507 2781 3473 3566 2507
Size: 274 692 93 4903
Fragments arranged by size:
4903 692 274 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2385 2527 2988 3167 3281 4023
Size: 399 397 198 142 461 179 114 742
Cuts at: 4023 4108 5179 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 461 399 397 198 179
142 114 85
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 3851 5581 3851
Size: 1730 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2133 2587 2686 3317 844
Size: 1289 454 99 631 3489
Fragments arranged by size:
3489 1289 631 454 99
BsrBI CCG'CTC
Cuts at: 1394 1823 2764 3849 1394
Size: 429 941 1085 3507
Fragments arranged by size:
3507 1085 941 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4606 4793 1700
Size: 110 2796 187 2869
Fragments arranged by size:
2869 2796 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 4022 5034 4022
Size: 1012 4950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2368 2593 794
Size: 1574 225 4163
Fragments arranged by size:
4163 1574 225
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstXI CCAn_nnnn'nTGG
Cuts at: 2311 2311
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 2788 3292 3659 4044 4061 5513 5599 5611
Size: 816 504 367 385 17 1452 86 12
Cuts at: 5611 5957 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 816 504 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5410
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 2764 3478 4272 4292 2764
Size: 714 794 20 4434
Fragments arranged by size:
4434 794 714 20
ClaI AT'CG_AT
Cuts at: 1554 2847 1554
Size: 1293 4669
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DraIII CAC_nnn'GTG
Cuts at: 2593 2593
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2532 2829 2970
Size: 54 1425 50 275 18 702 297 141
Cuts at: 2970 3054 3409 4298 5927 8
Size: 84 355 889 1629 43
Fragments arranged by size:
1629 1425 889 702 355 297 275 141
84 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3409 1537
Size: 275 1597 4090
Fragments arranged by size:
4090 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2085 2342 2384 2669 2720 3892 1353
Size: 665 67 257 42 285 51 1172 3423
Fragments arranged by size:
3423 1172 665 285 257 67 51 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2933 2948 4549 4923 5069 387
Size: 1064 138 1344 15 1601 374 146 1280
Fragments arranged by size:
1601 1344 1280 1064 374 146 138 15
EcoICRI GAG'CTC
Cuts at: 2185 2986 2185
Size: 801 5161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2141 2189 2351 2366 2408 2534
Size: 393 483 26 48 162 15 42 126
Cuts at: 2534 2576 2789 2876 2927 3023 3053 4025 4622
Size: 42 213 87 51 96 30 972 597
Cuts at: 4622 5409 5677 1239
Size: 787 268 1524
Fragments arranged by size:
1524 972 787 597 483 393 268 213
162 126 96 87 51 48 42 42
30 26 15
EcoO109I rG'GnC_Cy
Cuts at: 2821 3100 3214 2821
Size: 279 114 5569
Fragments arranged by size:
5569 279 114
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
EcoRV GAT'ATC
Cuts at: 2923 2923
Size: 5962
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 5962
FspI TGC'GCA
Cuts at: 2612 4468 2612
Size: 1856 4106
HaeII r_GCGC'y
Cuts at: 1678 2145 2164 2230 2576 2656 5109 1678
Size: 467 19 66 346 80 2453 2531
Fragments arranged by size:
2531 2453 467 346 80 66 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2181 2213 2408 2440 2461
Size: 32 135 32 609 32 195 32 21
Cuts at: 2461 2493 4603 4635 4677 4709 1373
Size: 32 2110 32 42 32 2626
Fragments arranged by size:
2626 2110 609 195 135 42 32 32
32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5076 5260 1305
Size: 3771 184 2007
Fragments arranged by size:
3771 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2831 10
Size: 54 1425 343 999 3141
Fragments arranged by size:
3141 1425 999 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3920 4279 4438 518
Size: 648 2754 359 159 2042
Fragments arranged by size:
2754 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2554 2608 2782 3001 3010 3042 3378 4055
Size: 948 54 174 219 9 32 336 677
Cuts at: 4055 5203 5448 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 948 677 336 245 219 174
54 32 9
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5410
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 2617 3086 3245 3320 4865 1132
Size: 97 1388 469 159 75 1545 2229
Fragments arranged by size:
2229 1545 1388 469 159 97 75
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5962
PciI A'CATG_T
Cuts at: 3241 4861 3241
Size: 1620 4342
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2512 2629 1108
Size: 1404 117 4441
Fragments arranged by size:
4441 1404 117
PfoI T'CCnGG_A
Cuts at: 2295 2295
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2261 2293 4031 4063 5544 5576 2261
Size: 32 1738 32 1481 32 2647
Fragments arranged by size:
2647 1738 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PspOMI G'GGCC_C
Cuts at: 2523 2523
Size: 5962
PstI C_TGCA'G
Cuts at: 830 2466 2580 2994 3015 3237 3314 830
Size: 1636 114 414 21 222 77 3478
Fragments arranged by size:
3478 1636 414 222 114 77 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
PvuII CAG'CTG
Cuts at: 2554 2608 2782 3001 3010 3042 2554
Size: 54 174 219 9 32 5474
Fragments arranged by size:
5474 219 174 54 32 9
SacI G_AGCT'C
Cuts at: 2187 2988 2187
Size: 801 5161
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SapI GCTCTTCn'nnn_
Cuts at: 2018 2720 2018
Size: 702 5260
SbfI CC_TGCA'GG
Cuts at: 2994 3314 2994
Size: 320 5642
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 2462 2576 2990 3011 3233 3310 4445
Size: 219 1417 114 414 21 222 77 1135
Cuts at: 4445 5126 5317 826
Size: 681 191 1471
Fragments arranged by size:
1471 1417 1135 681 414 222 219 191
114 77 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 2531 2819 2828 2531
Size: 288 9 5665
Fragments arranged by size:
5665 288 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 2528 2960 3289 1872
Size: 656 432 329 4545
Fragments arranged by size:
4545 656 432 329
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4066 4967 5229 820
Size: 197 926 2123 901 262 1553
Fragments arranged by size:
2123 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 2617 3320 1132
Size: 1485 703 3774
Fragments arranged by size:
3774 1485 703
SrfI GCCC'GGGC
Cuts at: 2528 2528
Size: 5962
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 2423 1517
Size: 906 5056
StyI C'CwwG_G
Cuts at: 513 1065 3401 513
Size: 552 2336 3074
Fragments arranged by size:
3074 2336 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3989 4006 4159 4344 4789 325
Size: 1255 2409 17 153 185 445 1498
Fragments arranged by size:
2409 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3021 4208 5920
Size: 274 80 33 51 1443 1044 1187 1712
Cuts at: 5920 96
Size: 138
Fragments arranged by size:
1712 1443 1187 1044 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5922 5954 5922
Size: 32 5930
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 2311
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 2526 2958 3287 1870
Size: 656 432 329 4545
Fragments arranged by size:
4545 656 432 329
XmnI GAAnn'nnTTC
Cuts at: 2276 4091 2276
Size: 1815 4147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2445 3770 276
Size: 53 83 186 936 911 1325 2468
Fragments arranged by size:
2468 1325 936 911 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SexAI SfoI SwaI