Restriction map for
pFN21AB5452
(Circular) (Six-base) MAPSORT of: pFN21AB5452.seq Check: 4794 from: 1 to: 5971
pFN21AB5452 5971 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1994 2825 4034 5418 5686 1632
Size: 362 831 1209 1384 268 1917
Fragments arranged by size:
1917 1384 1209 831 362 268
AflII C'TTAA_G
Cuts at: 820 1017 2581 820
Size: 197 1564 4210
Fragments arranged by size:
4210 1564 197
AflIII A'CryG_T
Cuts at: 1203 2766 3268 4870 5847 1203
Size: 1563 502 1602 977 1327
Fragments arranged by size:
1602 1563 1327 977 502
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AlwNI CAG_nnn'CTG
Cuts at: 2486 3714 5286 2486
Size: 1228 1572 3171
Fragments arranged by size:
3171 1572 1228
ApaLI G'TGCA_C
Cuts at: 4028 5184 4028
Size: 1156 4815
ApoI r'AATT_y
Cuts at: 1493 2398 3280 3506 3658 5767 1493
Size: 905 882 226 152 2109 1697
Fragments arranged by size:
2109 1697 905 882 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2424 4525 160
Size: 872 1392 2101 1606
Fragments arranged by size:
2101 1606 1392 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 3296 1870
Size: 73 1353 4545
Fragments arranged by size:
4545 1353 73
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3292 4747 618
Size: 325 217 627 1505 1455 1842
Fragments arranged by size:
1842 1505 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3290 1391
Size: 1899 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2029 2926 928
Size: 971 130 897 3973
Fragments arranged by size:
3973 971 897 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4162 4196 1903
Size: 34 50 34 2141 34 3678
Fragments arranged by size:
3678 2141 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3014 3863 5073 3014
Size: 849 1210 3912
Fragments arranged by size:
3912 1210 849
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2812 2968 5966 2812
Size: 156 2998 2817
Fragments arranged by size:
2998 2817 156
BlpI GC'TnA_GC
Cuts at: 3388 3388
Size: 5971
Bme1580I G_kGCm'C
Cuts at: 1790 4032 5188 1790
Size: 2242 1156 2573
Fragments arranged by size:
2573 2242 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2722 3702 4660
Size: 416 53 83 399 24 1305 980 958
Cuts at: 4660 442
Size: 1753
Fragments arranged by size:
1753 1305 980 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2360 2985 4631 1239
Size: 1121 625 1646 2579
Fragments arranged by size:
2579 1646 1121 625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4096 4961 5259 4096
Size: 865 298 4808
Fragments arranged by size:
4808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2070 4634 882
Size: 617 571 2564 2219
Fragments arranged by size:
2564 2219 617 571
BsaAI yAC'GTr
Cuts at: 493 1204 2673 3762 493
Size: 711 1469 1089 2702
Fragments arranged by size:
2702 1469 1089 711
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3778 4160 4795 275
Size: 53 83 186 936 2245 382 635 1451
Fragments arranged by size:
2245 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2219 2249 1178
Size: 30 165 30 816 30 4900
Fragments arranged by size:
4900 816 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2032 1186
Size: 846 5125
BseYI C'CCAG_C
Cuts at: 5174 5670 5174
Size: 496 5475
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5398
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4032 4117 5188 4032
Size: 85 1071 4815
Fragments arranged by size:
4815 1071 85
BsmI GAATG_Cn'
Cuts at: 2059 3482 3575 2059
Size: 1423 93 4455
Fragments arranged by size:
4455 1423 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3290 4032 4117 5188 1391
Size: 399 1500 742 85 1071 2174
Fragments arranged by size:
2174 1500 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 2140 2641 3860 5590 2140
Size: 501 1219 1730 2521
Fragments arranged by size:
2521 1730 1219 501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3237 3326 844
Size: 2393 89 3489
Fragments arranged by size:
3489 2393 89
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2536 4466 4640 65
Size: 1183 1288 1930 174 1396
Fragments arranged by size:
1930 1396 1288 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4615 4802 1700
Size: 110 2805 187 2869
Fragments arranged by size:
2869 2805 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5971
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 2843 1184
Size: 1659 4312
BstYI r'GATC_y
Cuts at: 1972 2812 2968 3039 3301 3668 4053 4070 5522
Size: 840 156 71 262 367 385 17 1452
Cuts at: 5522 5608 5620 5966 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 840 385 367 346 262 156
86 71 17 12
Bsu36I CC'TnA_GG
Cuts at: 2276 2276
Size: 5971
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5419
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5232
Fragments arranged by size:
5232 683 56
BtsI GCAGTG_nn'
Cuts at: 2026 3487 4281 4301 2026
Size: 1461 794 20 3696
Fragments arranged by size:
3696 1461 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 2989 3275 2989
Size: 286 5685
DraIII CAC_nnn'GTG
Cuts at: 2354 2354
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2771 4978 809
Size: 507 1455 2207 1802
Fragments arranged by size:
2207 1802 1455 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936
Size: 54 1425 50 275 18 1588 889 1629
Cuts at: 5936 8
Size: 43
Fragments arranged by size:
1629 1588 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2500 3901 1353
Size: 1147 1401 3423
Fragments arranged by size:
3423 1401 1147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2996 4558 4932 5078 387
Size: 1064 138 1407 1562 374 146 1280
Fragments arranged by size:
1562 1407 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1994 2360 2825 2985 4034 4631 5418
Size: 393 362 366 465 160 1049 597 787
Cuts at: 5418 5686 1239
Size: 268 1524
Fragments arranged by size:
1524 1049 787 597 465 393 366 362
268 160
EcoRI G'AATT_C
Cuts at: 3280 3658 3280
Size: 378 5593
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 5118 1678
Size: 3440 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3049 3081 4612 4644 4686
Size: 32 135 32 1477 32 1531 32 42
Cuts at: 4686 4718 1373
Size: 32 2626
Fragments arranged by size:
2626 1531 1477 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2211 2776 3315 3561 669
Size: 988 281 273 565 539 246 3079
Fragments arranged by size:
3079 988 565 539 281 273 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5085 5269 1305
Size: 3780 184 2007
Fragments arranged by size:
3780 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4149
Fragments arranged by size:
4149 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3929 4288 4447 518
Size: 648 2763 359 159 2042
Fragments arranged by size:
2763 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3387 4064 5212 5457 1606
Size: 1781 677 1148 245 2120
Fragments arranged by size:
2120 1781 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5419
NdeI CA'TA_TG
Cuts at: 387 2743 387
Size: 2356 3615
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 2770 2899 3272 3329 4874 1132
Size: 97 1541 129 373 57 1545 2229
Fragments arranged by size:
2229 1545 1541 373 129 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5971
PciI A'CATG_T
Cuts at: 2766 3268 4870 2766
Size: 502 1602 3867
Fragments arranged by size:
3867 1602 502
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3077 3109 4040 4072 5553 5585 3077
Size: 32 931 32 1481 32 3463
Fragments arranged by size:
3463 1481 931 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PstI C_TGCA'G
Cuts at: 830 2482 3323 830
Size: 1652 841 3478
Fragments arranged by size:
3478 1652 841
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SapI GCTCTTCn'nnn_
Cuts at: 2500 2500
Size: 5971
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 2094 4219 5931 2094
Size: 2125 1712 2134
Fragments arranged by size:
2134 2125 1712
SexAI A'CCwGG_T
Cuts at: 2545 2545
Size: 5971
SfcI C'TryA_G
Cuts at: 826 1045 2478 3319 4454 5135 5326 826
Size: 219 1433 841 1135 681 191 1471
Fragments arranged by size:
1471 1433 1135 841 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2581 4075 4976 5238 820
Size: 197 926 638 1494 901 262 1553
Fragments arranged by size:
1553 1494 926 901 638 262 197
SnaBI TAC'GTA
Cuts at: 493 2673 3762 493
Size: 2180 1089 2702
Fragments arranged by size:
2702 2180 1089
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 2568 2786 3895 5
Size: 47 2516 218 1109 2081
Fragments arranged by size:
2516 2081 1109 218 47
StuI AGG'CCT
Cuts at: 1517 2281 2337 1517
Size: 764 56 5151
Fragments arranged by size:
5151 764 56
StyI C'CwwG_G
Cuts at: 513 1065 3410 513
Size: 552 2345 3074
Fragments arranged by size:
3074 2345 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2196 2881 3998 4015 4168 4353 4798
Size: 1255 616 685 1117 17 153 185 445
Cuts at: 4798 325
Size: 1498
Fragments arranged by size:
1498 1255 1117 685 616 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2092 4217 5929
Size: 274 80 33 51 1443 115 2125 1712
Cuts at: 5929 96
Size: 138
Fragments arranged by size:
2125 1712 1443 274 138 115 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5931 5963 5931
Size: 32 5939
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 4100 4100
Size: 5971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI XcmI