Restriction map for
pFN21AB5447
(Circular) (Six-base) MAPSORT of: pFN21AB5447.seq Check: 708 from: 1 to: 5944
pFN21AB5447 5944 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3754 278
Size: 53 83 186 936 2218 2468
Fragments arranged by size:
2468 2218 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3287 1937
Size: 1350 4594
Acc65I G'GTAC_C
Cuts at: 3265 3265
Size: 5944
AclI AA'CG_TT
Cuts at: 1563 4071 4444 1563
Size: 2508 373 3063
Fragments arranged by size:
3063 2508 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2062 2763 4007 5391 5659 1632
Size: 430 701 1244 1384 268 1917
Fragments arranged by size:
1917 1384 1244 701 430 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5747
AflIII A'CryG_T
Cuts at: 1203 4843 5820 1203
Size: 3640 977 1327
Fragments arranged by size:
3640 1327 977
AgeI A'CCGG_T
Cuts at: 2649 4775 2649
Size: 2126 3818
AhdI GACnn_n'nnGTC
Cuts at: 2473 4673 2473
Size: 2200 3744
AleI CACnn'nnGTG
Cuts at: 1166 2577 1166
Size: 1411 4533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2202 1184
Size: 1018 4926
AlwNI CAG_nnn'CTG
Cuts at: 3687 5259 3687
Size: 1572 4372
ApaLI G'TGCA_C
Cuts at: 2337 2580 4001 5157 2337
Size: 243 1421 1156 3124
Fragments arranged by size:
3124 1421 1156 243
ApoI r'AATT_y
Cuts at: 1493 3253 3479 3631 5740 1493
Size: 1760 226 152 2109 1697
Fragments arranged by size:
2109 1760 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4498 160
Size: 872 3466 1606
Fragments arranged by size:
3466 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
AvaI C'yCGr_G
Cuts at: 1870 1943 3269 1870
Size: 73 1326 4545
Fragments arranged by size:
4545 1326 73
BamHI G'GATC_C
Cuts at: 2086 3274 3641 2086
Size: 1188 367 4389
Fragments arranged by size:
4389 1188 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 3265 4720 618
Size: 325 217 627 221 1257 1455 1842
Fragments arranged by size:
1842 1455 1257 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2793 2952 3263 1391
Size: 1402 159 311 4072
Fragments arranged by size:
4072 1402 311 159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2050 2984 928
Size: 971 151 934 3888
Fragments arranged by size:
3888 971 934 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4135 4169 1903
Size: 34 2198 34 3678
Fragments arranged by size:
3678 2198 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3836 5046 3836
Size: 1210 4734
BclI T'GATC_A
Cuts at: 1548 3070 1548
Size: 1522 4422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4555 136
Size: 107 122 71 631 605 2883 1525
Fragments arranged by size:
2883 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3008 5939 3008
Size: 2931 3013
BlpI GC'TnA_GC
Cuts at: 3361 3361
Size: 5944
Bme1580I G_kGCm'C
Cuts at: 1790 2341 2584 2767 3025 4005 5161 1790
Size: 551 243 183 258 980 1156 2573
Fragments arranged by size:
2573 1156 980 551 258 243 183
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5944
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2497 2604 2761
Size: 416 53 83 399 24 1080 107 157
Cuts at: 2761 3105 3675 4633 442
Size: 344 570 958 1753
Fragments arranged by size:
1753 1080 958 570 416 399 344 157
107 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 2558 2726 4604 1239
Size: 1262 57 168 1878 2579
Fragments arranged by size:
2579 1878 1262 168 57
Bpu10I CC'TnA_GC
Cuts at: 2946 2946
Size: 5944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2276 4069 4934 5232 2276
Size: 1793 865 298 2988
Fragments arranged by size:
2988 1793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4607 882
Size: 617 3108 2219
Fragments arranged by size:
3108 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3205 3735 493
Size: 711 2001 530 2702
Fragments arranged by size:
2702 2001 711 530
BsaBI GATnn'nnATC
Cuts at: 5938 5938
Size: 5944
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3751 4133 4768 275
Size: 53 83 186 936 2218 382 635 1451
Fragments arranged by size:
2218 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2089 2244 2611 2649 2991 4377 4775 5049 5196
Size: 155 367 38 342 1386 398 274 147
Cuts at: 5196 5607 2089
Size: 411 2426
Fragments arranged by size:
2426 1386 411 398 367 342 274 155
147 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5719
Fragments arranged by size:
5719 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5944
BseYI C'CCAG_C
Cuts at: 2043 2284 5147 5643 2043
Size: 241 2863 496 2344
Fragments arranged by size:
2863 2344 496 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5371
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3394 4155 5183 1540
Size: 275 186 1393 761 1028 2301
Fragments arranged by size:
2301 1393 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2341 2584 2917 2952 4005 4090 5161 2341
Size: 243 333 35 1053 85 1071 3124
Fragments arranged by size:
3124 1071 1053 333 243 85 35
BsmI GAATG_Cn'
Cuts at: 2548 3455 3548 2548
Size: 907 93 4944
Fragments arranged by size:
4944 907 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2341 2584 2767 2793 2917 2952 3025
Size: 399 551 243 183 26 124 35 73
Cuts at: 3025 3263 4005 4090 5161 1391
Size: 238 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 551 399 243 238 183
124 85 73 35 26
BspEI T'CCGG_A
Cuts at: 2089 2244 2611 5607 2089
Size: 155 367 2996 2426
Fragments arranged by size:
2996 2426 367 155
BspHI T'CATG_A
Cuts at: 2640 3833 5563 2640
Size: 1193 1730 3021
Fragments arranged by size:
3021 1730 1193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3103 3156 3299 844
Size: 2259 53 143 3489
Fragments arranged by size:
3489 2259 143 53
BsrBI CCG'CTC
Cuts at: 1394 1823 3831 1394
Size: 429 2008 3507
Fragments arranged by size:
3507 2008 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4439 4613 65
Size: 1183 3191 174 1396
Fragments arranged by size:
3191 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2649 4588 4775 1700
Size: 110 839 1939 187 2869
Fragments arranged by size:
2869 1939 839 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5944
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5944
BssSI C'ACGA_G
Cuts at: 2210 4004 5016 2210
Size: 1794 1012 3138
Fragments arranged by size:
3138 1794 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5944
BstBI TT'CG_AA
Cuts at: 2344 4748 2344
Size: 2404 3540
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5944
BstYI r'GATC_y
Cuts at: 1972 2086 3008 3274 3641 4026 4043 5495 5581
Size: 114 922 266 367 385 17 1452 86
Cuts at: 5581 5593 5939 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 922 385 367 346 266 114
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4453
Fragments arranged by size:
4453 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5205
Fragments arranged by size:
5205 683 56
BtsI GCAGTG_nn'
Cuts at: 2067 2624 3460 4254 4274 2067
Size: 557 836 794 20 3737
Fragments arranged by size:
3737 836 794 557 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5944
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3157 3192 529
Size: 35 2593 35 3281
Fragments arranged by size:
3281 2593 35 35
DraI TTT'AAA
Cuts at: 2464 3248 2464
Size: 784 5160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4951 809
Size: 507 3635 1802
Fragments arranged by size:
3635 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2925 3207 3391
Size: 54 1425 50 275 18 1095 282 184
Cuts at: 3391 4280 5909 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1095 889 282 275 184 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3391 1537
Size: 275 1579 4090
Fragments arranged by size:
4090 1579 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2495 3874 1353
Size: 1142 1379 3423
Fragments arranged by size:
3423 1379 1142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4531 4905 5051 387
Size: 1064 138 2942 374 146 1280
Fragments arranged by size:
2942 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2950 2950
Size: 5944
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2501 2558 2726 2763 4007 4604
Size: 393 430 439 57 168 37 1244 597
Cuts at: 4604 5391 5659 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1244 787 597 439 430 393 268
168 57 37
EcoO109I rG'GnC_Cy
Cuts at: 3051 3051
Size: 5944
EcoRI G'AATT_C
Cuts at: 3253 3631 3253
Size: 378 5566
FspI TGC'GCA
Cuts at: 4450 4450
Size: 5944
HaeII r_GCGC'y
Cuts at: 1678 5091 1678
Size: 3413 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 2499 2531 3000
Size: 32 135 32 887 32 8 32 469
Cuts at: 3000 3032 4585 4617 4659 4691 1373
Size: 32 1553 32 42 32 2626
Fragments arranged by size:
2626 1553 887 469 135 42 32 32
32 32 32 32 32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2107 3288 3534 669
Size: 988 281 169 1181 246 3079
Fragments arranged by size:
3079 1181 988 281 246 169
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5944
HpaI GTT'AAC
Cuts at: 3534 3534
Size: 5944
KpnI G_GTAC'C
Cuts at: 3269 3269
Size: 5944
MfeI C'AATT_G
Cuts at: 3543 3543
Size: 5944
MluI A'CGCG_T
Cuts at: 5820 5820
Size: 5944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2806 5058 5242 1305
Size: 1501 2252 184 2007
Fragments arranged by size:
2252 2007 1501 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2927 3209 10
Size: 54 1425 343 1095 282 2745
Fragments arranged by size:
2745 1425 1095 343 282 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2577 3902 4261 4420 518
Size: 648 1411 1325 359 159 2042
Fragments arranged by size:
2042 1411 1325 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2453 3360 4037 5185 5430 1606
Size: 847 907 677 1148 245 2120
Fragments arranged by size:
2120 1148 907 847 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4453
Fragments arranged by size:
4453 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5944
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5944
NotI GC'GGCC_GC
Cuts at: 3391 3391
Size: 5944
NspI r_CATG'y
Cuts at: 1132 1229 3085 3302 4847 1132
Size: 97 1856 217 1545 2229
Fragments arranged by size:
2229 1856 1545 217 97
PasI CC'CwG_GG
Cuts at: 1348 2148 2908 3047 1348
Size: 800 760 139 4245
Fragments arranged by size:
4245 800 760 139
PciI A'CATG_T
Cuts at: 4843 4843
Size: 5944
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5944
PmeI GTTT'AAAC
Cuts at: 3248 3248
Size: 5944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4013 4045 5526 5558 4013
Size: 32 1481 32 4399
Fragments arranged by size:
4399 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3051 3051
Size: 5944
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5944
PsiI TTA'TAA
Cuts at: 3514 3514
Size: 5944
PstI C_TGCA'G
Cuts at: 830 3296 830
Size: 2466 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5944
SacI G_AGCT'C
Cuts at: 2952 2952
Size: 5944
SalI G'TCGA_C
Cuts at: 1936 3286 1936
Size: 1350 4594
SbfI CC_TGCA'GG
Cuts at: 3296 3296
Size: 5944
ScaI AGT'ACT
Cuts at: 4192 5904 4192
Size: 1712 4232
SfcI C'TryA_G
Cuts at: 826 1045 3292 4427 5108 5299 826
Size: 219 2247 1135 681 191 1471
Fragments arranged by size:
2247 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5944
SmaI CCC'GGG
Cuts at: 1872 3271 1872
Size: 1399 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2291 4048 4949 5211 820
Size: 197 926 348 1757 901 262 1553
Fragments arranged by size:
1757 1553 926 901 348 262 197
SnaBI TAC'GTA
Cuts at: 493 3735 493
Size: 3242 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5944
SphI G_CATG'C
Cuts at: 1132 3302 1132
Size: 2170 3774
SspI AAT'ATT
Cuts at: 5 52 3868 5
Size: 47 3816 2081
Fragments arranged by size:
3816 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5944
StyI C'CwwG_G
Cuts at: 513 1065 2004 2269 3383 513
Size: 552 939 265 1114 3074
Fragments arranged by size:
3074 1114 939 552 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2463 3971 3988 4141 4326 4771 325
Size: 1255 883 1508 17 153 185 445 1498
Fragments arranged by size:
1508 1498 1255 883 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4190 5902 96
Size: 274 80 33 51 1443 2213 1712 138
Fragments arranged by size:
2213 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5904 5936 5904
Size: 32 5912
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5944
XbaI T'CTAG_A
Cuts at: 3196 3280 3196
Size: 84 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5944
XmaI C'CCGG_G
Cuts at: 1870 3269 1870
Size: 1399 4545
XmnI GAAnn'nnTTC
Cuts at: 4073 4073
Size: 5944
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3752 276
Size: 53 83 186 936 2218 2468
Fragments arranged by size:
2468 2218 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI