Restriction map for
pFN21AB5445
(Circular) (Six-base) MAPSORT of: pFN21AB5445.seq Check: 8950 from: 1 to: 5518
pFN21AB5445 5518 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2164 2801 2164
Size: 637 4881
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2724 3328 278
Size: 53 83 186 936 1188 604 2468
Fragments arranged by size:
2468 1188 936 604 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2861 1937
Size: 924 4594
Acc65I G'GTAC_C
Cuts at: 2839 2839
Size: 5518
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3581 4965 5233 1632
Size: 1949 1384 268 1917
Fragments arranged by size:
1949 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 4417 5394 1203
Size: 3214 977 1327
Fragments arranged by size:
3214 1327 977
AgeI A'CCGG_T
Cuts at: 4349 4349
Size: 5518
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5518
AlwNI CAG_nnn'CTG
Cuts at: 2197 2793 3261 4833 2197
Size: 596 468 1572 2882
Fragments arranged by size:
2882 1572 596 468
ApaI G_GGCC'C
Cuts at: 2029 2102 2138 2671 2029
Size: 73 36 533 4876
Fragments arranged by size:
4876 533 73 36
ApaLI G'TGCA_C
Cuts at: 3575 4731 3575
Size: 1156 4362
ApoI r'AATT_y
Cuts at: 1493 2827 3053 3205 5314 1493
Size: 1334 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4072 160
Size: 872 3040 1606
Fragments arranged by size:
3040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2116 2131 2843 1870
Size: 73 91 82 15 712 4545
Fragments arranged by size:
4545 712 91 82 73 15
BamHI G'GATC_C
Cuts at: 2848 3215 2848
Size: 367 5151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2387 2638 2839 4294 618
Size: 325 217 627 600 251 201 1455 1842
Fragments arranged by size:
1842 1455 627 600 325 251 217 201
BanII G_rGCy'C
Cuts at: 1391 2029 2102 2109 2138 2531 2671 2837 1391
Size: 638 73 7 29 393 140 166 4072
Fragments arranged by size:
4072 638 393 166 140 73 29 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4547
BbvCI CC'TCA_GC
Cuts at: 2618 2618
Size: 5518
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3709 3743 1903
Size: 34 50 34 1688 34 3678
Fragments arranged by size:
3678 1688 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2573 4129 136
Size: 107 122 71 631 605 901 1556 1525
Fragments arranged by size:
1556 1525 901 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5513 5513
Size: 5518
BlpI GC'TnA_GC
Cuts at: 2935 2935
Size: 5518
Bme1580I G_kGCm'C
Cuts at: 1790 2029 2035 2102 2138 2332 2583 2643 2671
Size: 239 6 67 36 194 251 60 28
Cuts at: 2671 3579 4735 1790
Size: 908 1156 2573
Fragments arranged by size:
2573 1156 908 251 239 194 67 60
36 28 6
BmgBI CAC'GTC
Cuts at: 1318 2713 1318
Size: 1395 4123
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3249 4207 442
Size: 416 53 83 399 24 1832 958 1753
Fragments arranged by size:
1832 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2735 2770 4178 1239
Size: 1496 35 1408 2579
Fragments arranged by size:
2579 1496 1408 35
Bpu10I CC'TnA_GC
Cuts at: 2618 2618
Size: 5518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4508 4806 3643
Size: 865 298 4355
Fragments arranged by size:
4355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4181 882
Size: 617 2682 2219
Fragments arranged by size:
2682 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3309 493
Size: 711 2105 2702
Fragments arranged by size:
2702 2105 711
BsaBI GATnn'nnATC
Cuts at: 2804 5512 2804
Size: 2708 2810
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2182 2315 2721 3325
Size: 53 83 186 936 649 133 406 604
Cuts at: 3325 3707 4342 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 649 635 604 406 382 186
133 83 53
BsaWI w'CCGG_w
Cuts at: 3951 4349 4623 4770 5181 3951
Size: 398 274 147 411 4288
Fragments arranged by size:
4288 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2711 2741 1178
Size: 30 165 30 1308 30 3955
Fragments arranged by size:
3955 1308 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 1186
Size: 1033 4485
BseYI C'CCAG_C
Cuts at: 2290 2531 4721 5217 2290
Size: 241 2190 496 2591
Fragments arranged by size:
2591 2190 496 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2510 1163
Size: 573 774 4171
Fragments arranged by size:
4171 774 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2577 2704 2968 3729 4757 1540
Size: 275 186 576 127 264 761 1028 2301
Fragments arranged by size:
2301 1028 761 576 275 264 186 127
BsiHKAI G_wGCw'C
Cuts at: 2439 2664 3579 3664 4735 2439
Size: 225 915 85 1071 3222
Fragments arranged by size:
3222 1071 915 225 85
BsiWI C'GTAC_G
Cuts at: 2517 2517
Size: 5518
BsmI GAATG_Cn'
Cuts at: 3029 3122 3029
Size: 93 5425
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2035 2102 2109 2138 2332 2439
Size: 399 239 6 67 7 29 194 107
Cuts at: 2439 2531 2583 2643 2664 2671 2837 3579 3664
Size: 92 52 60 21 7 166 742 85
Cuts at: 3664 4735 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 239 194 166 107
92 85 67 60 52 29 21 7
7 6
BspEI T'CCGG_A
Cuts at: 5181 5181
Size: 5518
BspHI T'CATG_A
Cuts at: 2439 2472 3407 5137 2439
Size: 33 935 1730 2820
Fragments arranged by size:
2820 1730 935 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2164 2801 2873 844
Size: 1320 637 72 3489
Fragments arranged by size:
3489 1320 637 72
BsrBI CCG'CTC
Cuts at: 1394 1823 2334 3405 1394
Size: 429 511 1071 3507
Fragments arranged by size:
3507 1071 511 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4013 4187 65
Size: 1183 2765 174 1396
Fragments arranged by size:
2765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2423 2483 4162 4349 1700
Size: 110 613 60 1679 187 2869
Fragments arranged by size:
2869 1679 613 187 110 60
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5518
BssSI C'ACGA_G
Cuts at: 3578 4590 3578
Size: 1012 4506
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5518
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5518
BstXI CCAn_nnnn'nTGG
Cuts at: 2290 2539 2290
Size: 249 5269
BstYI r'GATC_y
Cuts at: 1972 2848 3215 3600 3617 5069 5155 5167 5513
Size: 876 367 385 17 1452 86 12 346
Cuts at: 5513 1972
Size: 1977
Fragments arranged by size:
1977 1452 876 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2337 2365 2553 2598 513
Size: 552 1272 28 188 45 3433
Fragments arranged by size:
3433 1272 552 188 45 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2227 487
Size: 683 56 1001 3778
Fragments arranged by size:
3778 1001 683 56
BtsI GCAGTG_nn'
Cuts at: 3034 3828 3848 3034
Size: 794 20 4704
Fragments arranged by size:
4704 794 20
ClaI AT'CG_AT
Cuts at: 1554 2268 1554
Size: 714 4804
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5483
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DraIII CAC_nnn'GTG
Cuts at: 2629 2629
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4525 809
Size: 507 3209 1802
Fragments arranged by size:
3209 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 2305 2421
Size: 54 1425 50 275 18 432 43 116
Cuts at: 2421 2425 2481 2574 2701 2965 3854 5483 8
Size: 4 56 93 127 264 889 1629 43
Fragments arranged by size:
1629 1425 889 432 275 264 127 116
93 56 54 50 43 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2574 2701 2965 1537
Size: 275 762 127 264 4090
Fragments arranged by size:
4090 762 275 264 127
EarI CTCTTCn'nnn_
Cuts at: 1353 2402 3448 1353
Size: 1049 1046 3423
Fragments arranged by size:
3423 1049 1046
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4105 4479 4625 387
Size: 1064 138 2516 374 146 1280
Fragments arranged by size:
2516 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2735 2770 3581 4178 4965 5233 1239
Size: 393 1103 35 811 597 787 268 1524
Fragments arranged by size:
1524 1103 811 787 597 393 268 35
EcoO109I rG'GnC_Cy
Cuts at: 2098 2668 2098
Size: 570 4948
EcoRI G'AATT_C
Cuts at: 2827 3205 2827
Size: 378 5140
EcoRV GAT'ATC
Cuts at: 2272 2272
Size: 5518
FseI GG_CCGG'CC
Cuts at: 2427 2427
Size: 5518
FspI TGC'GCA
Cuts at: 4024 4024
Size: 5518
HaeII r_GCGC'y
Cuts at: 1678 4665 1678
Size: 2987 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2089 2376 2408 2711
Size: 32 135 32 485 32 287 32 303
Cuts at: 2711 2743 4159 4191 4233 4265 1373
Size: 32 1416 32 42 32 2626
Fragments arranged by size:
2626 1416 485 303 287 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 3108 669
Size: 988 281 924 246 3079
Fragments arranged by size:
3079 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5518
HpaI GTT'AAC
Cuts at: 3108 3108
Size: 5518
KpnI G_GTAC'C
Cuts at: 2843 2843
Size: 5518
MfeI C'AATT_G
Cuts at: 3117 3117
Size: 5518
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4632 4816 1305
Size: 3327 184 2007
Fragments arranged by size:
3327 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2264 10
Size: 54 1425 343 432 3264
Fragments arranged by size:
3264 1425 432 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2477 3476 3835 3994 518
Size: 648 1311 999 359 159 2042
Fragments arranged by size:
2042 1311 999 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2197 2221 2934 3611 4759 5004 1606
Size: 591 24 713 677 1148 245 2120
Fragments arranged by size:
2120 1148 713 677 591 245 24
NaeI GCC'GGC
Cuts at: 2425 2485 2425
Size: 60 5458
NcoI C'CATG_G
Cuts at: 513 1065 2337 2553 2598 513
Size: 552 1272 216 45 3433
Fragments arranged by size:
3433 1272 552 216 45
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NgoMIV G'CCGG_C
Cuts at: 2423 2483 2423
Size: 60 5458
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2965 2965
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2218 2876 4421 1132
Size: 97 989 658 1545 2229
Fragments arranged by size:
2229 1545 989 658 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5518
PciI A'CATG_T
Cuts at: 4417 4417
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2159 1108
Size: 1051 4467
PfoI T'CCnGG_A
Cuts at: 2463 2538 2463
Size: 75 5443
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 5100 5132 3587
Size: 32 1481 32 3973
Fragments arranged by size:
3973 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2185 1613
Size: 572 4946
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PspOMI G'GGCC_C
Cuts at: 2025 2098 2134 2667 2025
Size: 73 36 533 4876
Fragments arranged by size:
4876 533 73 36
PstI C_TGCA'G
Cuts at: 830 2870 830
Size: 2040 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
PvuII CAG'CTG
Cuts at: 2197 2221 2197
Size: 24 5494
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SapI GCTCTTCn'nnn_
Cuts at: 2402 2402
Size: 5518
SbfI CC_TGCA'GG
Cuts at: 2870 2870
Size: 5518
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SexAI A'CCwGG_T
Cuts at: 2625 2625
Size: 5518
SfcI C'TryA_G
Cuts at: 826 1045 2866 4001 4682 4873 826
Size: 219 1821 1135 681 191 1471
Fragments arranged by size:
1821 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5518
SmaI CCC'GGG
Cuts at: 1872 2036 2845 1872
Size: 164 809 4545
Fragments arranged by size:
4545 809 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3622 4523 4785 820
Size: 197 926 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2218 2876 1132
Size: 1086 658 3774
Fragments arranged by size:
3774 1086 658
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 2129 1517
Size: 612 4906
StyI C'CwwG_G
Cuts at: 513 1065 2091 2337 2553 2598 2957 513
Size: 552 1026 246 216 45 359 3074
Fragments arranged by size:
3074 1026 552 359 246 216 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3545 3562 3715 3900 4345 325
Size: 1255 1965 17 153 185 445 1498
Fragments arranged by size:
1965 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3764 5476 96
Size: 274 80 33 51 1443 1787 1712 138
Fragments arranged by size:
1787 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5478 5510 5478
Size: 32 5486
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2398 2398
Size: 5518
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5518
XmaI C'CCGG_G
Cuts at: 1870 2034 2843 1870
Size: 164 809 4545
Fragments arranged by size:
4545 809 164
XmnI GAAnn'nnTTC
Cuts at: 3647 3647
Size: 5518
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2722 3326 276
Size: 53 83 186 936 1188 604 2468
Fragments arranged by size:
2468 1188 936 604 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsmBI BstZ17I Bsu36I EcoICRI EcoNI FalI FspAI KasI
NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI