Restriction map for
pFN21AB5425
(Circular) (Six-base) MAPSORT of: pFN21AB5425.seq Check: 8759 from: 1 to: 5095
pFN21AB5425 5095 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2905 278
Size: 53 83 186 936 1369 2468
Fragments arranged by size:
2468 1369 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2438 1937
Size: 501 4594
Acc65I G'GTAC_C
Cuts at: 2416 2416
Size: 5095
AclI AA'CG_TT
Cuts at: 1563 3222 3595 1563
Size: 1659 373 3063
Fragments arranged by size:
3063 1659 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 3158 4542 4810 1632
Size: 653 873 1384 268 1917
Fragments arranged by size:
1917 1384 873 653 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4898
AflIII A'CryG_T
Cuts at: 1203 3994 4971 1203
Size: 2791 977 1327
Fragments arranged by size:
2791 1327 977
AgeI A'CCGG_T
Cuts at: 3926 3926
Size: 5095
AhdI GACnn_n'nnGTC
Cuts at: 3824 3824
Size: 5095
AleI CACnn'nnGTG
Cuts at: 1166 2237 1166
Size: 1071 4024
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5095
AlwNI CAG_nnn'CTG
Cuts at: 2136 2838 4410 2136
Size: 702 1572 2821
Fragments arranged by size:
2821 1572 702
ApaLI G'TGCA_C
Cuts at: 3152 4308 3152
Size: 1156 3939
ApoI r'AATT_y
Cuts at: 1493 2404 2630 2782 4891 1493
Size: 911 226 152 2109 1697
Fragments arranged by size:
2109 1697 911 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3649 160
Size: 872 2617 1606
Fragments arranged by size:
2617 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5095
AvaI C'yCGr_G
Cuts at: 1870 1943 2420 1870
Size: 73 477 4545
Fragments arranged by size:
4545 477 73
BamHI G'GATC_C
Cuts at: 2425 2792 2425
Size: 367 4728
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2416 3871 618
Size: 325 217 627 629 1455 1842
Fragments arranged by size:
1842 1455 629 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2414 1391
Size: 1023 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2097 928
Size: 971 198 3926
Fragments arranged by size:
3926 971 198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3286 3320 1903
Size: 34 1349 34 3678
Fragments arranged by size:
3678 1349 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2318 2987 4197 2318
Size: 669 1210 3216
Fragments arranged by size:
3216 1210 669
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5095
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3706 136
Size: 107 122 71 631 605 2034 1525
Fragments arranged by size:
2034 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2059 2208 2268 5090 2059
Size: 149 60 2822 2064
Fragments arranged by size:
2822 2064 149 60
BlpI GC'TnA_GC
Cuts at: 2512 2512
Size: 5095
Bme1580I G_kGCm'C
Cuts at: 1790 3156 4312 1790
Size: 1366 1156 2573
Fragments arranged by size:
2573 1366 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5095
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2307 2826 3784
Size: 416 53 83 399 24 890 519 958
Cuts at: 3784 442
Size: 1753
Fragments arranged by size:
1753 958 890 519 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 3755 1239
Size: 932 1584 2579
Fragments arranged by size:
2579 1584 932
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2057 3220 4085 4383 2057
Size: 1163 865 298 2769
Fragments arranged by size:
2769 1163 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3758 882
Size: 617 2259 2219
Fragments arranged by size:
2259 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2886 493
Size: 711 1682 2702
Fragments arranged by size:
2702 1682 711
BsaBI GATnn'nnATC
Cuts at: 5089 5089
Size: 5095
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2902 3284 3919 275
Size: 53 83 186 936 1369 382 635 1451
Fragments arranged by size:
1451 1369 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3528 3926 4200 4347 4758 3528
Size: 398 274 147 411 3865
Fragments arranged by size:
3865 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2168 2198 1178
Size: 30 165 30 765 30 4075
Fragments arranged by size:
4075 765 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2291 1186
Size: 943 162 3990
Fragments arranged by size:
3990 943 162
BseYI C'CCAG_C
Cuts at: 2099 4298 4794 2099
Size: 2199 496 2400
Fragments arranged by size:
2400 2199 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4522
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2545 3306 4334 1540
Size: 275 186 544 761 1028 2301
Fragments arranged by size:
2301 1028 761 544 275 186
BsiHKAI G_wGCw'C
Cuts at: 2199 3156 3241 4312 2199
Size: 957 85 1071 2982
Fragments arranged by size:
2982 1071 957 85
BsmI GAATG_Cn'
Cuts at: 2606 2699 2606
Size: 93 5002
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2199 2414 3156 3241 4312 1391
Size: 399 409 215 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 409 399 215 85
BspEI T'CCGG_A
Cuts at: 4758 4758
Size: 5095
BspHI T'CATG_A
Cuts at: 2984 4714 2984
Size: 1730 3365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2450 844
Size: 1606 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2982 1394
Size: 429 1159 3507
Fragments arranged by size:
3507 1159 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3590 3764 65
Size: 1183 2342 174 1396
Fragments arranged by size:
2342 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3739 3926 1700
Size: 110 1929 187 2869
Fragments arranged by size:
2869 1929 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5095
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5095
BssSI C'ACGA_G
Cuts at: 3155 4167 3155
Size: 1012 4083
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5095
BstBI TT'CG_AA
Cuts at: 3899 3899
Size: 5095
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5095
BstYI r'GATC_y
Cuts at: 1972 2059 2208 2268 2425 2792 3177 3194 4646
Size: 87 149 60 157 367 385 17 1452
Cuts at: 4646 4732 4744 5090 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 157 149 87
86 60 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3604
Fragments arranged by size:
3604 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4356
Fragments arranged by size:
4356 683 56
BtsI GCAGTG_nn'
Cuts at: 2137 2611 3405 3425 2137
Size: 474 794 20 3807
Fragments arranged by size:
3807 794 474 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5095
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5060
DraI TTT'AAA
Cuts at: 2399 2399
Size: 5095
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4102 809
Size: 507 2786 1802
Fragments arranged by size:
2786 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2542 3431 5060
Size: 54 1425 50 275 18 712 889 1629
Cuts at: 5060 8
Size: 43
Fragments arranged by size:
1629 1425 889 712 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2542 1537
Size: 275 730 4090
Fragments arranged by size:
4090 730 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2352 3025 1353
Size: 999 673 3423
Fragments arranged by size:
3423 999 673
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3682 4056 4202 387
Size: 1064 138 2093 374 146 1280
Fragments arranged by size:
2093 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2285 3158 3755 4542 4810 1239
Size: 393 539 114 873 597 787 268 1524
Fragments arranged by size:
1524 873 787 597 539 393 268 114
EcoO109I rG'GnC_Cy
Cuts at: 2019 2019
Size: 5095
EcoRI G'AATT_C
Cuts at: 2404 2782 2404
Size: 378 4717
FspI TGC'GCA
Cuts at: 3601 3601
Size: 5095
HaeII r_GCGC'y
Cuts at: 1678 4242 1678
Size: 2564 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2168 2200 3736 3768 3810
Size: 32 135 32 596 32 1536 32 42
Cuts at: 3810 3842 1373
Size: 32 2626
Fragments arranged by size:
2626 1536 596 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2245 2439 2685 669
Size: 988 281 307 194 246 3079
Fragments arranged by size:
3079 988 307 281 246 194
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5095
HpaI GTT'AAC
Cuts at: 2685 2685
Size: 5095
KpnI G_GTAC'C
Cuts at: 2420 2420
Size: 5095
MfeI C'AATT_G
Cuts at: 2694 2694
Size: 5095
MluI A'CGCG_T
Cuts at: 4971 4971
Size: 5095
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4209 4393 1305
Size: 2904 184 2007
Fragments arranged by size:
2904 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3273
Fragments arranged by size:
3273 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 3053 3412 3571 518
Size: 648 1071 816 359 159 2042
Fragments arranged by size:
2042 1071 816 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2511 3188 4336 4581 1606
Size: 905 677 1148 245 2120
Fragments arranged by size:
2120 1148 905 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3604
Fragments arranged by size:
3604 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5095
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5095
NotI GC'GGCC_GC
Cuts at: 2542 2542
Size: 5095
NspI r_CATG'y
Cuts at: 1132 1229 2453 3998 1132
Size: 97 1224 1545 2229
Fragments arranged by size:
2229 1545 1224 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5095
PciI A'CATG_T
Cuts at: 3994 3994
Size: 5095
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5095
PfoI T'CCnGG_A
Cuts at: 2148 2148
Size: 5095
PmeI GTTT'AAAC
Cuts at: 2399 2399
Size: 5095
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 4677 4709 3164
Size: 32 1481 32 3550
Fragments arranged by size:
3550 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2019 2019
Size: 5095
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5095
PsiI TTA'TAA
Cuts at: 2665 2665
Size: 5095
PstI C_TGCA'G
Cuts at: 830 2447 830
Size: 1617 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5095
SalI G'TCGA_C
Cuts at: 1936 2437 1936
Size: 501 4594
SanDI GG'GwC_CC
Cuts at: 2019 2019
Size: 5095
SbfI CC_TGCA'GG
Cuts at: 2447 2447
Size: 5095
ScaI AGT'ACT
Cuts at: 3343 5055 3343
Size: 1712 3383
SfcI C'TryA_G
Cuts at: 826 1045 2248 2443 3578 4259 4450 826
Size: 219 1203 195 1135 681 191 1471
Fragments arranged by size:
1471 1203 1135 681 219 195 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5095
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5095
SmaI CCC'GGG
Cuts at: 1872 2422 1872
Size: 550 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2072 3199 4100 4362 820
Size: 197 926 129 1127 901 262 1553
Fragments arranged by size:
1553 1127 926 901 262 197 129
SnaBI TAC'GTA
Cuts at: 493 2886 493
Size: 2393 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5095
SphI G_CATG'C
Cuts at: 1132 2453 1132
Size: 1321 3774
SspI AAT'ATT
Cuts at: 5 52 3019 5
Size: 47 2967 2081
Fragments arranged by size:
2967 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5095
StyI C'CwwG_G
Cuts at: 513 1065 2004 2534 513
Size: 552 939 530 3074
Fragments arranged by size:
3074 939 552 530
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3122 3139 3292 3477 3922 325
Size: 1255 1542 17 153 185 445 1498
Fragments arranged by size:
1542 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 3341 5053
Size: 274 80 33 51 1443 348 1016 1712
Cuts at: 5053 96
Size: 138
Fragments arranged by size:
1712 1443 1016 348 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5055 5087 5055
Size: 32 5063
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5095
XbaI T'CTAG_A
Cuts at: 2062 2431 2062
Size: 369 4726
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5095
XmaI C'CCGG_G
Cuts at: 1870 2420 1870
Size: 550 4545
XmnI GAAnn'nnTTC
Cuts at: 3224 3224
Size: 5095
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2903 276
Size: 53 83 186 936 1369 2468
Fragments arranged by size:
2468 1369 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI