Restriction map for
pFN21AB5420
(Circular) (Six-base) MAPSORT of: pFN21AB5420.seq Check: 5107 from: 1 to: 6007
pFN21AB5420 6007 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3817 278
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3350 1937
Size: 1413 4594
Acc65I G'GTAC_C
Cuts at: 3328 3328
Size: 6007
AclI AA'CG_TT
Cuts at: 1563 4134 4507 1563
Size: 2571 373 3063
Fragments arranged by size:
3063 2571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2080 2448 2521 2600 2705 2743 3050 3153
Size: 448 368 73 79 105 38 307 103
Cuts at: 3153 3271 4070 5454 5722 1632
Size: 118 799 1384 268 1917
Fragments arranged by size:
1917 1384 799 448 368 307 268 118
105 103 79 73 38
AfeI AGC'GCT
Cuts at: 3212 3212
Size: 6007
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 4906 5883 1203
Size: 3703 977 1327
Fragments arranged by size:
3703 1327 977
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 2788 4736 2788
Size: 1948 4059
AleI CACnn'nnGTG
Cuts at: 1166 2621 1166
Size: 1455 4552
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6007
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2993 3025 2993
Size: 32 5975
AlwNI CAG_nnn'CTG
Cuts at: 3750 5322 3750
Size: 1572 4435
ApaLI G'TGCA_C
Cuts at: 3169 4064 5220 3169
Size: 895 1156 3956
Fragments arranged by size:
3956 1156 895
ApoI r'AATT_y
Cuts at: 1493 3316 3542 3694 5803 1493
Size: 1823 226 152 2109 1697
Fragments arranged by size:
2109 1823 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4561 160
Size: 872 3529 1606
Fragments arranged by size:
3529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 3332 1870
Size: 73 1389 4545
Fragments arranged by size:
4545 1389 73
BamHI G'GATC_C
Cuts at: 3337 3704 3337
Size: 367 5640
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2369 3191 3328 4783 618
Size: 325 217 627 582 822 137 1455 1842
Fragments arranged by size:
1842 1455 822 627 582 325 217 137
BanII G_rGCy'C
Cuts at: 1391 2151 3326 1391
Size: 760 1175 4072
Fragments arranged by size:
4072 1175 760
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2693 928
Size: 971 794 4242
Fragments arranged by size:
4242 971 794
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4198 4232 1903
Size: 34 2261 34 3678
Fragments arranged by size:
3678 2261 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3899 5109 3899
Size: 1210 4797
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6007
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4618 136
Size: 107 122 71 631 605 2946 1525
Fragments arranged by size:
2946 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2034 6002 2034
Size: 3968 2039
BlpI GC'TnA_GC
Cuts at: 3424 3424
Size: 6007
Bme1580I G_kGCm'C
Cuts at: 1790 2112 3173 3196 4068 5224 1790
Size: 322 1061 23 872 1156 2573
Fragments arranged by size:
2573 1156 1061 872 322 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2106 2513 2861
Size: 416 53 83 399 24 689 407 348
Cuts at: 2861 2914 3183 3738 4696 442
Size: 53 269 555 958 1753
Fragments arranged by size:
1753 958 689 555 416 407 399 348
269 83 53 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2678 2696 4667 1239
Size: 1439 18 1971 2579
Fragments arranged by size:
2579 1971 1439 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2000 4132 4997 5295 2000
Size: 2132 865 298 2712
Fragments arranged by size:
2712 2132 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2096 4670 882
Size: 617 597 2574 2219
Fragments arranged by size:
2574 2219 617 597
BsaAI yAC'GTr
Cuts at: 493 1204 3798 493
Size: 711 2594 2702
Fragments arranged by size:
2702 2594 711
BsaBI GATnn'nnATC
Cuts at: 6001 6001
Size: 6007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3102 3814 4196 4831
Size: 53 83 186 936 1569 712 382 635
Cuts at: 4831 275
Size: 1451
Fragments arranged by size:
1569 1451 936 712 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4440 4838 5112 5259 5670 4440
Size: 398 274 147 411 4777
Fragments arranged by size:
4777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2550 2580 1178
Size: 30 165 30 1147 30 4605
Fragments arranged by size:
4605 1147 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3224 3263 1186
Size: 2038 39 3930
Fragments arranged by size:
3930 2038 39
BseYI C'CCAG_C
Cuts at: 2804 3253 5210 5706 2804
Size: 449 1957 496 3105
Fragments arranged by size:
3105 1957 496 449
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2547 2955 1163
Size: 573 811 408 4215
Fragments arranged by size:
4215 811 573 408
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3457 4218 5246 1540
Size: 275 186 1456 761 1028 2301
Fragments arranged by size:
2301 1456 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2151 2208 3173 4068 4153 5224 2151
Size: 57 965 895 85 1071 2934
Fragments arranged by size:
2934 1071 965 895 85 57
BsmI GAATG_Cn'
Cuts at: 3518 3611 3518
Size: 93 5914
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2151 2208 3173 3196 3326 4068
Size: 399 322 39 57 965 23 130 742
Cuts at: 4068 4153 5224 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 965 742 399 322 130 85
57 39 23
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 2508 3896 5626 2508
Size: 1388 1730 2889
Fragments arranged by size:
2889 1730 1388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2799 3362 844
Size: 1955 563 3489
Fragments arranged by size:
3489 1955 563
BsrBI CCG'CTC
Cuts at: 1394 1823 3894 1394
Size: 429 2071 3507
Fragments arranged by size:
3507 2071 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4502 4676 65
Size: 1183 3254 174 1396
Fragments arranged by size:
3254 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3275 4651 4838 1700
Size: 110 1465 1376 187 2869
Fragments arranged by size:
2869 1465 1376 187 110
BsrGI T'GTAC_A
Cuts at: 96 2841 96
Size: 2745 3262
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 4067 5079 4067
Size: 1012 4995
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6007
BstBI TT'CG_AA
Cuts at: 4811 4811
Size: 6007
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6007
BstXI CCAn_nnnn'nTGG
Cuts at: 2623 2656 3092 2623
Size: 33 436 5538
Fragments arranged by size:
5538 436 33
BstYI r'GATC_y
Cuts at: 1972 2019 2034 2884 3337 3704 4089 4106 5558
Size: 47 15 850 453 367 385 17 1452
Cuts at: 5558 5644 5656 6002 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 850 453 385 367 346 86
47 17 15 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5455
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2389 3130 487
Size: 683 56 1163 741 3364
Fragments arranged by size:
3364 1163 741 683 56
BtsI GCAGTG_nn'
Cuts at: 3523 4317 4337 3523
Size: 794 20 5193
Fragments arranged by size:
5193 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 3311 3311
Size: 6007
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5014 809
Size: 507 3698 1802
Fragments arranged by size:
3698 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2493 3216 3300
Size: 54 1425 50 275 18 663 723 84
Cuts at: 3300 3454 4343 5972 8
Size: 154 889 1629 43
Fragments arranged by size:
1629 1425 889 723 663 275 154 84
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3454 1537
Size: 275 1642 4090
Fragments arranged by size:
4090 1642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2099 3069 3240 3937 1353
Size: 728 18 970 171 697 3423
Fragments arranged by size:
3423 970 728 697 171 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4594 4968 5114 387
Size: 1064 138 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2149 2149
Size: 6007
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2448 2521 2600 2678 2696 2705
Size: 393 448 368 73 79 78 18 9
Cuts at: 2705 2743 3050 3153 3271 4070 4667 5454 5722
Size: 38 307 103 118 799 597 787 268
Cuts at: 5722 1239
Size: 1524
Fragments arranged by size:
1524 799 787 597 448 393 368 307
268 118 103 79 78 73 38 18
9
EcoO109I rG'GnC_Cy
Cuts at: 2140 2386 2140
Size: 246 5761
EcoRI G'AATT_C
Cuts at: 3316 3694 3316
Size: 378 5629
FspI TGC'GCA
Cuts at: 2552 4513 2552
Size: 1961 4046
FspAI rTGC'GCAy
Cuts at: 2552 2552
Size: 6007
HaeII r_GCGC'y
Cuts at: 1678 3214 5154 1678
Size: 1536 1940 2531
Fragments arranged by size:
2531 1940 1536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2774 2806 3046 3078 4648
Size: 32 135 32 1202 32 240 32 1570
Cuts at: 4648 4680 4722 4754 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1570 1202 240 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3037 3351 3597 669
Size: 988 281 1099 314 246 3079
Fragments arranged by size:
3079 1099 988 314 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6007
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KpnI G_GTAC'C
Cuts at: 3332 3332
Size: 6007
MfeI C'AATT_G
Cuts at: 3606 3606
Size: 6007
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5121 5305 1305
Size: 3816 184 2007
Fragments arranged by size:
3816 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2495 3218 3302 10
Size: 54 1425 343 663 723 84 2715
Fragments arranged by size:
2715 1425 723 663 343 84 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2621 2732 3965 4324 4483 518
Size: 648 1455 111 1233 359 159 2042
Fragments arranged by size:
2042 1455 1233 648 359 159 111
MspA1I CmG'CkG
Cuts at: 1606 2830 3423 4100 5248 5493 1606
Size: 1224 593 677 1148 245 2120
Fragments arranged by size:
2120 1224 1148 677 593 245
NaeI GCC'GGC
Cuts at: 3277 3277
Size: 6007
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5455
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6007
NgoMIV G'CCGG_C
Cuts at: 3275 3275
Size: 6007
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 3365 4910 1132
Size: 97 2136 1545 2229
Fragments arranged by size:
2229 2136 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6007
PciI A'CATG_T
Cuts at: 4906 4906
Size: 6007
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2950 3096 1108
Size: 1842 146 4019
Fragments arranged by size:
4019 1842 146
PfoI T'CCnGG_A
Cuts at: 2151 2655 2151
Size: 504 5503
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5589 5621 4076
Size: 32 1481 32 4462
Fragments arranged by size:
4462 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2386 2386
Size: 6007
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6007
PsiI TTA'TAA
Cuts at: 3577 3577
Size: 6007
PstI C_TGCA'G
Cuts at: 830 3359 830
Size: 2529 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
PvuII CAG'CTG
Cuts at: 2830 2830
Size: 6007
SacI G_AGCT'C
Cuts at: 2151 2151
Size: 6007
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SanDI GG'GwC_CC
Cuts at: 2386 2386
Size: 6007
SbfI CC_TGCA'GG
Cuts at: 3359 3359
Size: 6007
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SexAI A'CCwGG_T
Cuts at: 3153 3153
Size: 6007
SfcI C'TryA_G
Cuts at: 826 1045 3355 4490 5171 5362 826
Size: 219 2310 1135 681 191 1471
Fragments arranged by size:
2310 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6007
SmaI CCC'GGG
Cuts at: 1872 3334 1872
Size: 1462 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2015 4111 5012 5274 820
Size: 197 926 72 2096 901 262 1553
Fragments arranged by size:
2096 1553 926 901 262 197 72
SnaBI TAC'GTA
Cuts at: 493 3798 493
Size: 3305 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 3365 1132
Size: 2233 3774
SspI AAT'ATT
Cuts at: 5 52 3931 5
Size: 47 3879 2081
Fragments arranged by size:
3879 2081 47
StuI AGG'CCT
Cuts at: 1517 2673 1517
Size: 1156 4851
StyI C'CwwG_G
Cuts at: 513 1065 2991 3297 3303 3446 513
Size: 552 1926 306 6 143 3074
Fragments arranged by size:
3074 1926 552 306 143 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3071 4034 4051 4204 4389 4834 325
Size: 1255 1491 963 17 153 185 445 1498
Fragments arranged by size:
1498 1491 1255 963 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2841 3222 4253
Size: 274 80 33 51 1443 864 381 1031
Cuts at: 4253 5965 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1031 864 381 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5967 5999 5967
Size: 32 5975
Tth111I GACn'n_nGTC
Cuts at: 1316 3058 1316
Size: 1742 4265
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6007
XmaI C'CCGG_G
Cuts at: 1870 3332 1870
Size: 1462 4545
XmnI GAAnn'nnTTC
Cuts at: 4136 4136
Size: 6007
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3815 276
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SapI SfiI
SfoI SrfI SwaI XcmI