Restriction map for
pFN21AB5418
(Circular) (Six-base) MAPSORT of: pFN21AB5418.seq Check: 6961 from: 1 to: 6157
pFN21AB5418 6157 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3967 278
Size: 53 83 186 936 2431 2468
Fragments arranged by size:
2468 2431 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2212 2560 2976 3500 1937
Size: 275 348 416 524 4594
Fragments arranged by size:
4594 524 416 348 275
Acc65I G'GTAC_C
Cuts at: 3478 3478
Size: 6157
AclI AA'CG_TT
Cuts at: 1563 4284 4657 1563
Size: 2721 373 3063
Fragments arranged by size:
3063 2721 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3069 3332 3464 4220 5604 5872 1632
Size: 1437 263 132 756 1384 268 1917
Fragments arranged by size:
1917 1437 1384 756 268 263 132
AfeI AGC'GCT
Cuts at: 2132 2132
Size: 6157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5960
AflIII A'CryG_T
Cuts at: 1203 2563 5056 6033 1203
Size: 1360 2493 977 1327
Fragments arranged by size:
2493 1360 1327 977
AgeI A'CCGG_T
Cuts at: 4988 4988
Size: 6157
AhdI GACnn_n'nnGTC
Cuts at: 4886 4886
Size: 6157
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6157
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2014 2046 2014
Size: 32 6125
AlwNI CAG_nnn'CTG
Cuts at: 3900 5472 3900
Size: 1572 4585
ApaLI G'TGCA_C
Cuts at: 4214 5370 4214
Size: 1156 5001
ApoI r'AATT_y
Cuts at: 1493 2833 3317 3466 3692 3844 5953 1493
Size: 1340 484 149 226 152 2109 1697
Fragments arranged by size:
2109 1697 1340 484 226 152 149
AseI AT'TA_AT
Cuts at: 160 1032 4711 160
Size: 872 3679 1606
Fragments arranged by size:
3679 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6157
AvaI C'yCGr_G
Cuts at: 1870 1943 3482 1870
Size: 73 1539 4545
Fragments arranged by size:
4545 1539 73
AvrII C'CTAG_G
Cuts at: 3064 3064
Size: 6157
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3006 3039 3006
Size: 33 6124
BamHI G'GATC_C
Cuts at: 3487 3854 3487
Size: 367 5790
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3478 4933 618
Size: 325 217 627 1691 1455 1842
Fragments arranged by size:
1842 1691 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3476 1391
Size: 2085 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2307 928
Size: 971 408 4778
Fragments arranged by size:
4778 971 408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4348 4382 1903
Size: 34 2411 34 3678
Fragments arranged by size:
3678 2411 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4049 5259 4049
Size: 1210 4947
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6157
BfrBI ATG'CAT
Cuts at: 3105 3105
Size: 6157
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4768 136
Size: 107 122 71 631 605 3096 1525
Fragments arranged by size:
3096 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6152 6152
Size: 6157
BlpI GC'TnA_GC
Cuts at: 3574 3574
Size: 6157
Bme1580I G_kGCm'C
Cuts at: 1790 2119 4218 5374 1790
Size: 329 2099 1156 2573
Fragments arranged by size:
2573 2099 1156 329
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6157
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3268 3888 4846
Size: 416 53 83 399 24 1851 620 958
Cuts at: 4846 442
Size: 1753
Fragments arranged by size:
1851 1753 958 620 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3112 4817 1239
Size: 1873 1705 2579
Fragments arranged by size:
2579 1873 1705
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2232 3315 4282 5147 5445 2232
Size: 1083 967 865 298 2944
Fragments arranged by size:
2944 1083 967 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2271 4820 882
Size: 617 772 2549 2219
Fragments arranged by size:
2549 2219 772 617
BsaAI yAC'GTr
Cuts at: 493 1204 3948 493
Size: 711 2744 2702
Fragments arranged by size:
2744 2702 711
BsaBI GATnn'nnATC
Cuts at: 6151 6151
Size: 6157
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3964 4346 4981 275
Size: 53 83 186 936 2431 382 635 1451
Fragments arranged by size:
2431 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4590 4988 5262 5409 5820 4590
Size: 398 274 147 411 4927
Fragments arranged by size:
4927 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5932
Fragments arranged by size:
5932 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2086 2453 1186
Size: 900 367 4890
Fragments arranged by size:
4890 900 367
BseYI C'CCAG_C
Cuts at: 5360 5856 5360
Size: 496 5661
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2915 1163
Size: 573 1179 4405
Fragments arranged by size:
4405 1179 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3250 3607 4368 5396 1540
Size: 275 186 1249 357 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 357 275 186
BsiHKAI G_wGCw'C
Cuts at: 3046 4218 4303 5374 3046
Size: 1172 85 1071 3829
Fragments arranged by size:
3829 1172 1071 85
BsmI GAATG_Cn'
Cuts at: 3105 3668 3761 3105
Size: 563 93 5501
Fragments arranged by size:
5501 563 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2460 3356 2460
Size: 896 5261
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2119 3046 3476 4218 4303 5374 1391
Size: 399 329 927 430 742 85 1071 2174
Fragments arranged by size:
2174 1071 927 742 430 399 329 85
BspEI T'CCGG_A
Cuts at: 5820 5820
Size: 6157
BspHI T'CATG_A
Cuts at: 4046 5776 4046
Size: 1730 4427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2172 3512 844
Size: 1328 1340 3489
Fragments arranged by size:
3489 1340 1328
BsrBI CCG'CTC
Cuts at: 1394 1823 4044 1394
Size: 429 2221 3507
Fragments arranged by size:
3507 2221 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3177 4652 4826 65
Size: 1183 1929 1475 174 1396
Fragments arranged by size:
1929 1475 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2289 3278 4801 4988 1700
Size: 110 479 989 1523 187 2869
Fragments arranged by size:
2869 1523 989 479 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6157
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6157
BssSI C'ACGA_G
Cuts at: 4217 5229 4217
Size: 1012 5145
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3102 794
Size: 2308 3849
BstBI TT'CG_AA
Cuts at: 4961 4961
Size: 6157
BstEII G'GTnAC_C
Cuts at: 1184 2444 1184
Size: 1260 4897
BstYI r'GATC_y
Cuts at: 1972 3487 3854 4239 4256 5708 5794 5806 6152
Size: 1515 367 385 17 1452 86 12 346
Cuts at: 6152 1972
Size: 1977
Fragments arranged by size:
1977 1515 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5605
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2252 487
Size: 683 56 1026 4392
Fragments arranged by size:
4392 1026 683 56
BtsI GCAGTG_nn'
Cuts at: 2119 3673 4467 4487 2119
Size: 1554 794 20 3789
Fragments arranged by size:
3789 1554 794 20
ClaI AT'CG_AT
Cuts at: 1554 2541 3250 1554
Size: 987 709 4461
Fragments arranged by size:
4461 987 709
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6122
DraI TTT'AAA
Cuts at: 3461 3461
Size: 6157
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5164 809
Size: 507 3848 1802
Fragments arranged by size:
3848 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3604 4493 6122
Size: 54 1425 50 275 18 1774 889 1629
Cuts at: 6122 8
Size: 43
Fragments arranged by size:
1774 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3604 1537
Size: 275 1792 4090
Fragments arranged by size:
4090 1792 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 2390 4087 1353
Size: 740 297 1697 3423
Fragments arranged by size:
3423 1697 740 297
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4744 5118 5264 387
Size: 1064 138 3155 374 146 1280
Fragments arranged by size:
3155 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3069 3112 3332 3464 4220 4817 5604
Size: 393 1437 43 220 132 756 597 787
Cuts at: 5604 5872 1239
Size: 268 1524
Fragments arranged by size:
1524 1437 787 756 597 393 268 220
132 43
EcoRI G'AATT_C
Cuts at: 3466 3844 3466
Size: 378 5779
FspI TGC'GCA
Cuts at: 4663 4663
Size: 6157
HaeII r_GCGC'y
Cuts at: 1678 2134 5304 1678
Size: 456 3170 2531
Fragments arranged by size:
3170 2531 456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2797 2829 4798 4830 4872
Size: 32 135 32 1225 32 1969 32 42
Cuts at: 4872 4904 1373
Size: 32 2626
Fragments arranged by size:
2626 1969 1225 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2213 2856 3501 3747 669
Size: 988 281 275 643 645 246 3079
Fragments arranged by size:
3079 988 645 643 281 275 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6157
HpaI GTT'AAC
Cuts at: 3747 3747
Size: 6157
KpnI G_GTAC'C
Cuts at: 3482 3482
Size: 6157
MfeI C'AATT_G
Cuts at: 3756 3756
Size: 6157
MluI A'CGCG_T
Cuts at: 6033 6033
Size: 6157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2667 3167 3357 5271 5455 1305
Size: 1362 500 190 1914 184 2007
Fragments arranged by size:
2007 1914 1362 500 190 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4335
Fragments arranged by size:
4335 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2112 4115 4474 4633 518
Size: 648 946 2003 359 159 2042
Fragments arranged by size:
2042 2003 946 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2673 3573 4250 5398 5643 1606
Size: 1067 900 677 1148 245 2120
Fragments arranged by size:
2120 1148 1067 900 677 245
NaeI GCC'GGC
Cuts at: 3280 3280
Size: 6157
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5605
NdeI CA'TA_TG
Cuts at: 387 2995 3207 387
Size: 2608 212 3337
Fragments arranged by size:
3337 2608 212
NgoMIV G'CCGG_C
Cuts at: 3278 3278
Size: 6157
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6157
NotI GC'GGCC_GC
Cuts at: 3604 3604
Size: 6157
NsiI A_TGCA'T
Cuts at: 3107 3107
Size: 6157
NspI r_CATG'y
Cuts at: 1132 1229 2567 3515 5060 1132
Size: 97 1338 948 1545 2229
Fragments arranged by size:
2229 1545 1338 948 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6157
PciI A'CATG_T
Cuts at: 2563 5056 2563
Size: 2493 3664
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2173 2686 1108
Size: 1065 513 4579
Fragments arranged by size:
4579 1065 513
PmeI GTTT'AAAC
Cuts at: 3461 3461
Size: 6157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4226 4258 5739 5771 4226
Size: 32 1481 32 4612
Fragments arranged by size:
4612 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6157
PsiI TTA'TAA
Cuts at: 2343 3727 2343
Size: 1384 4773
PstI C_TGCA'G
Cuts at: 830 3509 830
Size: 2679 3478
PvuI CG_AT'CG
Cuts at: 2001 3250 2001
Size: 1249 4908
SalI G'TCGA_C
Cuts at: 1936 2211 3499 1936
Size: 275 1288 4594
Fragments arranged by size:
4594 1288 275
SbfI CC_TGCA'GG
Cuts at: 3509 3509
Size: 6157
ScaI AGT'ACT
Cuts at: 2371 2665 2746 4405 6117 2371
Size: 294 81 1659 1712 2411
Fragments arranged by size:
2411 1712 1659 294 81
SfcI C'TryA_G
Cuts at: 826 1045 3505 4640 5321 5512 826
Size: 219 2460 1135 681 191 1471
Fragments arranged by size:
2460 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6157
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6157
SmaI CCC'GGG
Cuts at: 1872 3484 1872
Size: 1612 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2247 3330 4261 5162 5424 820
Size: 197 926 304 1083 931 901 262 1553
Fragments arranged by size:
1553 1083 931 926 901 304 262 197
SnaBI TAC'GTA
Cuts at: 493 3948 493
Size: 3455 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6157
SphI G_CATG'C
Cuts at: 1132 3515 1132
Size: 2383 3774
SspI AAT'ATT
Cuts at: 5 52 3236 4081 5
Size: 47 3184 845 2081
Fragments arranged by size:
3184 2081 845 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6157
StyI C'CwwG_G
Cuts at: 513 1065 3064 3596 513
Size: 552 1999 532 3074
Fragments arranged by size:
3074 1999 552 532
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4184 4201 4354 4539 4984 325
Size: 1255 2604 17 153 185 445 1498
Fragments arranged by size:
2604 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2229 2369 2663
Size: 274 80 33 51 1443 252 140 294
Cuts at: 2663 2744 3014 4403 6115 96
Size: 81 270 1389 1712 138
Fragments arranged by size:
1712 1443 1389 294 274 270 252 140
138 81 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2514 2546 6117 6149 2514
Size: 32 3571 32 2522
Fragments arranged by size:
3571 2522 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6157
XbaI T'CTAG_A
Cuts at: 3493 3493
Size: 6157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3087 3087
Size: 6157
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6157
XmaI C'CCGG_G
Cuts at: 1870 3482 1870
Size: 1612 4545
XmnI GAAnn'nnTTC
Cuts at: 3183 4286 3183
Size: 1103 5054
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3965 276
Size: 53 83 186 936 2431 2468
Fragments arranged by size:
2468 2431 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI BbeI BbvCI BplI Bpu10I BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NarI NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI