Restriction map for
pFN21AB5414
(Circular) (Six-base) MAPSORT of: pFN21AB5414.seq Check: 9234 from: 1 to: 5728
pFN21AB5414 5728 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2500 2500
Size: 5728
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3538 278
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3071 1937
Size: 1134 4594
Acc65I G'GTAC_C
Cuts at: 2594 3049 2594
Size: 455 5273
AclI AA'CG_TT
Cuts at: 1563 3855 4228 1563
Size: 2292 373 3063
Fragments arranged by size:
3063 2292 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2469 2493 2862 3791 5175 5443 1632
Size: 837 24 369 929 1384 268 1917
Fragments arranged by size:
1917 1384 929 837 369 268 24
AflII C'TTAA_G
Cuts at: 820 1017 2999 820
Size: 197 1982 3549
Fragments arranged by size:
3549 1982 197
AflIII A'CryG_T
Cuts at: 1203 4627 5604 1203
Size: 3424 977 1327
Fragments arranged by size:
3424 1327 977
AgeI A'CCGG_T
Cuts at: 4559 4559
Size: 5728
AhdI GACnn_n'nnGTC
Cuts at: 4457 4457
Size: 5728
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5728
AlwNI CAG_nnn'CTG
Cuts at: 2063 2492 2614 3471 5043 2063
Size: 429 122 857 1572 2748
Fragments arranged by size:
2748 1572 857 429 122
ApaLI G'TGCA_C
Cuts at: 3785 4941 3785
Size: 1156 4572
ApoI r'AATT_y
Cuts at: 1493 2382 2412 3037 3263 3415 5524 1493
Size: 889 30 625 226 152 2109 1697
Fragments arranged by size:
2109 1697 889 625 226 152 30
AseI AT'TA_AT
Cuts at: 160 1032 4282 160
Size: 872 3250 1606
Fragments arranged by size:
3250 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
AvaI C'yCGr_G
Cuts at: 1870 1943 2895 3053 1870
Size: 73 952 158 4545
Fragments arranged by size:
4545 952 158 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2578 2611 2578
Size: 33 5695
BamHI G'GATC_C
Cuts at: 3058 3425 3058
Size: 367 5361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2512 2594 3049 4504 618
Size: 325 217 627 725 82 455 1455 1842
Fragments arranged by size:
1842 1455 725 627 455 325 217 82
BanII G_rGCy'C
Cuts at: 1391 3047 1391
Size: 1656 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2259 928
Size: 971 360 4397
Fragments arranged by size:
4397 971 360
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3919 3953 1903
Size: 34 1982 34 3678
Fragments arranged by size:
3678 1982 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3620 4830 3620
Size: 1210 4518
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5728
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2185 4339 136
Size: 107 122 71 631 605 513 2154 1525
Fragments arranged by size:
2154 1525 631 605 513 122 107 71
BglII A'GATC_T
Cuts at: 2700 5723 2700
Size: 3023 2705
BlpI GC'TnA_GC
Cuts at: 3145 3145
Size: 5728
Bme1580I G_kGCm'C
Cuts at: 1790 2712 3789 4945 1790
Size: 922 1077 1156 2573
Fragments arranged by size:
2573 1156 1077 922
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5728
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3459 4417 442
Size: 416 53 83 399 24 2042 958 1753
Fragments arranged by size:
2042 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2040 2763 2817 4388 1239
Size: 801 723 54 1571 2579
Fragments arranged by size:
2579 1571 801 723 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3853 4718 5016 3853
Size: 865 298 4565
Fragments arranged by size:
4565 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4391 882
Size: 617 2892 2219
Fragments arranged by size:
2892 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3519 493
Size: 711 2315 2702
Fragments arranged by size:
2702 2315 711
BsaBI GATnn'nnATC
Cuts at: 5722 5722
Size: 5728
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3535 3917 4552 275
Size: 53 83 186 936 2002 382 635 1451
Fragments arranged by size:
2002 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2297 4161 4559 4833 4980 5391 2297
Size: 1864 398 274 147 411 2634
Fragments arranged by size:
2634 1864 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5503
Fragments arranged by size:
5503 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2492 2583 2999 1186
Size: 1306 91 416 3915
Fragments arranged by size:
3915 1306 416 91
BseYI C'CCAG_C
Cuts at: 2091 2223 2711 2818 4931 5427 2091
Size: 132 488 107 2113 496 2392
Fragments arranged by size:
2392 2113 496 488 132 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5155
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3178 3939 4967 1540
Size: 275 186 1177 761 1028 2301
Fragments arranged by size:
2301 1177 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3789 3874 4945 3789
Size: 85 1071 4572
Fragments arranged by size:
4572 1071 85
BsmI GAATG_Cn'
Cuts at: 3239 3332 3239
Size: 93 5635
BsmBI CGTCTCn'nnnn_
Cuts at: 2739 2739
Size: 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2712 3047 3789 3874 4945 1391
Size: 399 922 335 742 85 1071 2174
Fragments arranged by size:
2174 1071 922 742 399 335 85
BspEI T'CCGG_A
Cuts at: 2297 5391 2297
Size: 3094 2634
BspHI T'CATG_A
Cuts at: 3617 5347 3617
Size: 1730 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2500 3083 844
Size: 1656 583 3489
Fragments arranged by size:
3489 1656 583
BsrBI CCG'CTC
Cuts at: 1394 1823 3615 1394
Size: 429 1792 3507
Fragments arranged by size:
3507 1792 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4223 4397 65
Size: 1183 2975 174 1396
Fragments arranged by size:
2975 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4372 4559 1700
Size: 110 2562 187 2869
Fragments arranged by size:
2869 2562 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5728
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5728
BssSI C'ACGA_G
Cuts at: 3788 4800 3788
Size: 1012 4716
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2138 794
Size: 1344 4384
BstBI TT'CG_AA
Cuts at: 4532 4532
Size: 5728
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5728
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2039 2011
Size: 28 5700
BstYI r'GATC_y
Cuts at: 1972 2700 3058 3425 3810 3827 5279 5365 5377
Size: 728 358 367 385 17 1452 86 12
Cuts at: 5377 5723 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 728 385 367 358 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2010 2203 513
Size: 552 945 193 4038
Fragments arranged by size:
4038 945 552 193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4989
Fragments arranged by size:
4989 683 56
BtsI GCAGTG_nn'
Cuts at: 2545 2958 3244 4038 4058 2545
Size: 413 286 794 20 4215
Fragments arranged by size:
4215 794 413 286 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5693
DraI TTT'AAA
Cuts at: 3032 3032
Size: 5728
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4735 809
Size: 507 3419 1802
Fragments arranged by size:
3419 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3175 4064 5693
Size: 54 1425 50 275 18 1345 889 1629
Cuts at: 5693 8
Size: 43
Fragments arranged by size:
1629 1425 1345 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3175 1537
Size: 275 1363 4090
Fragments arranged by size:
4090 1363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2457 2469 3658 1353
Size: 1104 12 1189 3423
Fragments arranged by size:
3423 1189 1104 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4315 4689 4835 387
Size: 1064 138 2726 374 146 1280
Fragments arranged by size:
2726 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2469 2493 2763 2817 2862 3791
Size: 393 408 429 24 270 54 45 929
Cuts at: 3791 4388 5175 5443 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 929 787 597 429 408 393 270
268 54 45 24
EcoO109I rG'GnC_Cy
Cuts at: 2611 3004 2611
Size: 393 5335
EcoRI G'AATT_C
Cuts at: 3037 3415 3037
Size: 378 5350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2415 2415
Size: 5728
FspI TGC'GCA
Cuts at: 4234 4234
Size: 5728
HaeII r_GCGC'y
Cuts at: 1678 4875 1678
Size: 3197 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2685 2717 4369 4401 4443
Size: 32 135 32 1113 32 1652 32 42
Cuts at: 4443 4475 1373
Size: 32 2626
Fragments arranged by size:
2626 1652 1113 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3072 3318 669
Size: 988 281 1134 246 3079
Fragments arranged by size:
3079 1134 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5728
HpaI GTT'AAC
Cuts at: 3318 3318
Size: 5728
KpnI G_GTAC'C
Cuts at: 2598 3053 2598
Size: 455 5273
MfeI C'AATT_G
Cuts at: 3327 3327
Size: 5728
MluI A'CGCG_T
Cuts at: 5604 5604
Size: 5728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4842 5026 1305
Size: 3537 184 2007
Fragments arranged by size:
3537 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3906
Fragments arranged by size:
3906 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3686 4045 4204 518
Size: 648 843 1677 359 159 2042
Fragments arranged by size:
2042 1677 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2176 2581 3144 3821 4969 5214 1606
Size: 445 125 405 563 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 563 445 405 245 125
NcoI C'CATG_G
Cuts at: 513 1065 2010 2203 513
Size: 552 945 193 4038
Fragments arranged by size:
4038 945 552 193
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5728
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5728
NotI GC'GGCC_GC
Cuts at: 3175 3175
Size: 5728
NspI r_CATG'y
Cuts at: 1132 1229 3086 4631 1132
Size: 97 1857 1545 2229
Fragments arranged by size:
2229 1857 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2563 1348
Size: 1215 4513
PciI A'CATG_T
Cuts at: 4627 4627
Size: 5728
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5728
PmeI GTTT'AAAC
Cuts at: 3032 3032
Size: 5728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 3829 5310 5342 3797
Size: 32 1481 32 4183
Fragments arranged by size:
4183 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3004 3004
Size: 5728
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5728
PsiI TTA'TAA
Cuts at: 3298 3298
Size: 5728
PstI C_TGCA'G
Cuts at: 830 3080 830
Size: 2250 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5728
PvuII CAG'CTG
Cuts at: 2051 2176 2581 2051
Size: 125 405 5198
Fragments arranged by size:
5198 405 125
SalI G'TCGA_C
Cuts at: 1936 3070 1936
Size: 1134 4594
SbfI CC_TGCA'GG
Cuts at: 3080 3080
Size: 5728
ScaI AGT'ACT
Cuts at: 3976 5688 3976
Size: 1712 4016
SfcI C'TryA_G
Cuts at: 826 1045 2404 2669 3076 4211 4892 5083 826
Size: 219 1359 265 407 1135 681 191 1471
Fragments arranged by size:
1471 1359 1135 681 407 265 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5728
SmaI CCC'GGG
Cuts at: 1872 3055 1872
Size: 1183 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2895 2999 3832 4733 4995 820
Size: 197 926 952 104 833 901 262 1553
Fragments arranged by size:
1553 952 926 901 833 262 197 104
SnaBI TAC'GTA
Cuts at: 493 3519 493
Size: 3026 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5728
SphI G_CATG'C
Cuts at: 1132 3086 1132
Size: 1954 3774
SspI AAT'ATT
Cuts at: 5 52 3652 5
Size: 47 3600 2081
Fragments arranged by size:
3600 2081 47
StuI AGG'CCT
Cuts at: 1517 2398 1517
Size: 881 4847
StyI C'CwwG_G
Cuts at: 513 1065 2010 2203 2450 3167 513
Size: 552 945 193 247 717 3074
Fragments arranged by size:
3074 945 717 552 247 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2791 3755 3772 3925 4110 4555 325
Size: 1255 1211 964 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1211 964 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2170 3974 5686
Size: 274 80 33 51 1443 193 1804 1712
Cuts at: 5686 96
Size: 138
Fragments arranged by size:
1804 1712 1443 274 193 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5688 5720 5688
Size: 32 5696
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5728
XbaI T'CTAG_A
Cuts at: 3064 3064
Size: 5728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2537 2537
Size: 5728
XhoI C'TCGA_G
Cuts at: 1943 2895 1943
Size: 952 4776
XmaI C'CCGG_G
Cuts at: 1870 3053 1870
Size: 1183 4545
XmnI GAAnn'nnTTC
Cuts at: 3857 3857
Size: 5728
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3536 276
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI