Restriction map for
pFN21AB5412
(Circular) (Six-base) MAPSORT of: pFN21AB5412.seq Check: 6545 from: 1 to: 5761
pFN21AB5412 5761 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 3082 3082
Size: 5761
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3824 5208 5476 1632
Size: 2192 1384 268 1917
Fragments arranged by size:
2192 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 4660 5637 1203
Size: 3457 977 1327
Fragments arranged by size:
3457 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5761
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2579 2611 2579
Size: 32 5729
AlwNI CAG_nnn'CTG
Cuts at: 2234 3002 3504 5076 2234
Size: 768 502 1572 2919
Fragments arranged by size:
2919 1572 768 502
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 2333 2421 2449 2799 3070 3296 3448 5557
Size: 840 88 28 350 271 226 152 2109
Cuts at: 5557 1493
Size: 1697
Fragments arranged by size:
2109 1697 840 350 271 226 152 88
28
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 2084 3086 1870
Size: 73 141 1002 4545
Fragments arranged by size:
4545 1002 141 73
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3082 4537 618
Size: 325 217 627 1295 1455 1842
Fragments arranged by size:
1842 1455 1295 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2154 3080 1391
Size: 763 926 4072
Fragments arranged by size:
4072 926 763
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4790
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 2497 1548
Size: 949 4812
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4372 136
Size: 107 122 71 631 605 2700 1525
Fragments arranged by size:
2700 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2869 5756 2869
Size: 2887 2874
BlpI GC'TnA_GC
Cuts at: 2546 3178 2546
Size: 632 5129
Bme1580I G_kGCm'C
Cuts at: 1790 2379 3822 4978 1790
Size: 589 1443 1156 2573
Fragments arranged by size:
2573 1443 1156 589
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3031 3492 4450
Size: 416 53 83 399 24 1614 461 958
Cuts at: 4450 442
Size: 1753
Fragments arranged by size:
1753 1614 958 461 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2513 2620 4421 1239
Size: 1274 107 1801 2579
Fragments arranged by size:
2579 1801 1274 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2038 2766 3886 4751 5049 2038
Size: 728 1120 865 298 2750
Fragments arranged by size:
2750 1120 865 728 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2136 4424 882
Size: 617 637 2288 2219
Fragments arranged by size:
2288 2219 637 617
BsaAI yAC'GTr
Cuts at: 493 1204 3552 493
Size: 711 2348 2702
Fragments arranged by size:
2702 2348 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3568 3950 4585 275
Size: 53 83 186 936 2035 382 635 1451
Fragments arranged by size:
2035 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4194 4592 4866 5013 5424 4194
Size: 398 274 147 411 4531
Fragments arranged by size:
4531 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2606 2636 1178
Size: 30 165 30 1203 30 4303
Fragments arranged by size:
4303 1203 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2138 2144 2702 2930 1186
Size: 949 3 6 558 228 4017
Fragments arranged by size:
4017 949 558 228 6 3
BseYI C'CCAG_C
Cuts at: 2643 4964 5460 2643
Size: 2321 496 2944
Fragments arranged by size:
2944 2321 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5188
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2154 3822 3907 4978 2154
Size: 1668 85 1071 2937
Fragments arranged by size:
2937 1668 1071 85
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
BsmBI CGTCTCn'nnnn_
Cuts at: 2093 2626 2093
Size: 533 5228
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2379 3080 3822 3907 4978 1391
Size: 399 364 225 701 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 701 399 364 225 85
BspEI T'CCGG_A
Cuts at: 5424 5424
Size: 5761
BspHI T'CATG_A
Cuts at: 3650 5380 3650
Size: 1730 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3116 844
Size: 2272 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3648 1394
Size: 429 1825 3507
Fragments arranged by size:
3507 1825 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2704 4256 4430 65
Size: 1183 1456 1552 174 1396
Fragments arranged by size:
1552 1456 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 4405 4592 1700
Size: 110 234 2361 187 2869
Fragments arranged by size:
2869 2361 234 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2204 2716 2896 794
Size: 1410 512 180 3659
Fragments arranged by size:
3659 1410 512 180
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 2869 3091 3458 3843 3860 5312 5398 5410
Size: 897 222 367 385 17 1452 86 12
Cuts at: 5410 5756 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 897 385 367 346 222 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2381 2511 513
Size: 552 1316 130 3763
Fragments arranged by size:
3763 1316 552 130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5022
Fragments arranged by size:
5022 683 56
BtsI GCAGTG_nn'
Cuts at: 3277 4071 4091 3277
Size: 794 20 4947
Fragments arranged by size:
4947 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3208 4097 5726
Size: 54 1425 50 275 18 1378 889 1629
Cuts at: 5726 8
Size: 43
Fragments arranged by size:
1629 1425 1378 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2337 2687 2928 3691 1353
Size: 984 350 241 763 3423
Fragments arranged by size:
3423 984 763 350 241
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4348 4722 4868 387
Size: 1064 138 2759 374 146 1280
Fragments arranged by size:
2759 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2152 2152
Size: 5761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2513 2620 3824 4421 5208 5476 1239
Size: 393 881 107 1204 597 787 268 1524
Fragments arranged by size:
1524 1204 881 787 597 393 268 107
EcoNI CCTnn'n_nnAGG
Cuts at: 2159 2861 2159
Size: 702 5059
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 4908 1678
Size: 3230 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 2550 2582 4402
Size: 32 135 32 506 32 440 32 1820
Cuts at: 4402 4434 4476 4508 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1820 506 440 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2389 3105 3351 669
Size: 988 281 451 716 246 3079
Fragments arranged by size:
3079 988 716 451 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KpnI G_GTAC'C
Cuts at: 3086 3086
Size: 5761
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4875 5059 1305
Size: 3570 184 2007
Fragments arranged by size:
3570 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3939
Fragments arranged by size:
3939 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3719 4078 4237 518
Size: 648 2553 359 159 2042
Fragments arranged by size:
2553 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2372 2438 2722 3177 3854 5002 5247 1606
Size: 766 66 284 455 677 1148 245 2120
Fragments arranged by size:
2120 1148 766 677 455 284 245 66
NcoI C'CATG_G
Cuts at: 513 1065 2381 513
Size: 552 1316 3893
Fragments arranged by size:
3893 1316 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 3119 4664 1132
Size: 97 1890 1545 2229
Fragments arranged by size:
2229 1890 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5761
PfoI T'CCnGG_A
Cuts at: 2529 2529
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2597 2629 2597
Size: 32 5729
PstI C_TGCA'G
Cuts at: 830 2133 2947 2967 3113 830
Size: 1303 814 20 146 3478
Fragments arranged by size:
3478 1303 814 146 20
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2372 2438 2372
Size: 66 5695
SacI G_AGCT'C
Cuts at: 2154 2154
Size: 5761
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SapI GCTCTTCn'nnn_
Cuts at: 2337 2687 2337
Size: 350 5411
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SfcI C'TryA_G
Cuts at: 826 1045 2129 2943 2963 3109 4244 4925 5116
Size: 219 1084 814 20 146 1135 681 191
Cuts at: 5116 826
Size: 1471
Fragments arranged by size:
1471 1135 1084 814 681 219 191 146
20
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 3088 1872
Size: 1216 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2017 2781 3865 4766 5028 820
Size: 197 926 74 764 1084 901 262 1553
Fragments arranged by size:
1553 1084 926 901 764 262 197 74
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 2893 152
Size: 2741 3020
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5761
StyI C'CwwG_G
Cuts at: 513 1065 2381 3200 513
Size: 552 1316 819 3074
Fragments arranged by size:
3074 1316 819 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3788 3805 3958 4143 4588 325
Size: 1255 2208 17 153 185 445 1498
Fragments arranged by size:
2208 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4007 5719 96
Size: 274 80 33 51 1443 2030 1712 138
Fragments arranged by size:
2030 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5721 5753 5721
Size: 32 5729
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 3086 1870
Size: 1216 4545
XmnI GAAnn'nnTTC
Cuts at: 2409 3890 2409
Size: 1481 4280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI