Restriction map for
pFN21AB5408
(Circular) (Six-base) MAPSORT of: pFN21AB5408.seq Check: 5924 from: 1 to: 5956
pFN21AB5408 5956 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2067 3299 1937
Size: 130 1232 4594
Fragments arranged by size:
4594 1232 130
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 4019 5403 5671 1632
Size: 413 1974 1384 268 1917
Fragments arranged by size:
1974 1917 1384 413 268
AflII C'TTAA_G
Cuts at: 820 1017 2654 820
Size: 197 1637 4122
Fragments arranged by size:
4122 1637 197
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2099 1184
Size: 915 5041
AlwNI CAG_nnn'CTG
Cuts at: 2281 2966 3699 5271 2281
Size: 685 733 1572 2966
Fragments arranged by size:
2966 1572 733 685
ApaLI G'TGCA_C
Cuts at: 2138 4013 5169 2138
Size: 1875 1156 2925
Fragments arranged by size:
2925 1875 1156
ApoI r'AATT_y
Cuts at: 1493 2431 2808 3265 3491 3643 5752 1493
Size: 938 377 457 226 152 2109 1697
Fragments arranged by size:
2109 1697 938 457 377 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2163 4510 160
Size: 872 1131 2347 1606
Fragments arranged by size:
2347 1606 1131 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 3281 1870
Size: 73 1338 4545
Fragments arranged by size:
4545 1338 73
AvrII C'CTAG_G
Cuts at: 2947 2947
Size: 5956
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2013 2020 2108 2551 2939
Size: 325 217 627 226 7 88 443 388
Cuts at: 2939 3277 4732 618
Size: 338 1455 1842
Fragments arranged by size:
1842 1455 627 443 388 338 325 226
217 88 7
BanII G_rGCy'C
Cuts at: 1391 2041 3087 3275 1391
Size: 650 1046 188 4072
Fragments arranged by size:
4072 1046 650 188
BbeI G_GCGC'C
Cuts at: 2017 2024 2112 2017
Size: 7 88 5861
Fragments arranged by size:
5861 88 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2610 928
Size: 971 711 4274
Fragments arranged by size:
4274 971 711
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2330 2364 4147 4181 1903
Size: 34 393 34 1783 34 3678
Fragments arranged by size:
3678 1783 393 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3160 4567 136
Size: 107 122 71 631 605 1488 1407 1525
Fragments arranged by size:
1525 1488 1407 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 2142 2556 2960 4017 5173 1790
Size: 352 414 404 1057 1156 2573
Fragments arranged by size:
2573 1156 1057 414 404 352
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2088 3687 4645
Size: 416 53 83 399 24 671 1599 958
Cuts at: 4645 442
Size: 1753
Fragments arranged by size:
1753 1599 958 671 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2538 2582 2594 2984 3215 4616 1239
Size: 1299 44 12 390 231 1401 2579
Fragments arranged by size:
2579 1401 1299 390 231 44 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2194 4081 4946 5244 2194
Size: 1887 865 298 2906
Fragments arranged by size:
2906 1887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4619 882
Size: 617 3120 2219
Fragments arranged by size:
3120 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3016 3747 493
Size: 711 1812 731 2702
Fragments arranged by size:
2702 1812 731 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2014 2021 2109 3763
Size: 53 83 186 936 481 7 88 1654
Cuts at: 3763 4145 4780 275
Size: 382 635 1451
Fragments arranged by size:
1654 1451 936 635 481 382 186 88
83 53 7
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2444 2474 1178
Size: 30 165 30 1041 30 4660
Fragments arranged by size:
4660 1041 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2118 1186
Size: 932 5024
BseYI C'CCAG_C
Cuts at: 2072 2371 2400 2555 5159 5655 2072
Size: 299 29 155 2604 496 2373
Fragments arranged by size:
2604 2373 496 299 155 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 2465 2681 1163
Size: 573 438 291 216 4438
Fragments arranged by size:
4438 573 438 291 216
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2142 3087 4017 4102 5173 2142
Size: 945 930 85 1071 2925
Fragments arranged by size:
2925 1071 945 930 85
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
BsmBI CGTCTCn'nnnn_
Cuts at: 2917 2917
Size: 5956
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2142 2556 2960 3087 3275 4017
Size: 399 251 101 414 404 127 188 742
Cuts at: 4017 4102 5173 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 414 404 399 251 188
127 101 85
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2745 2755 3311 844
Size: 1901 10 556 3489
Fragments arranged by size:
3489 1901 556 10
BsrBI CCG'CTC
Cuts at: 1394 1823 2393 3843 1394
Size: 429 570 1450 3507
Fragments arranged by size:
3507 1450 570 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4451 4625 65
Size: 1183 3203 174 1396
Fragments arranged by size:
3203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2010 2327 4600 4787 1700
Size: 110 200 317 2273 187 2869
Fragments arranged by size:
2869 2273 317 200 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 2405 4016 5028 2405
Size: 1611 1012 3333
Fragments arranged by size:
3333 1611 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2146 2443 3151 794
Size: 1352 297 708 3599
Fragments arranged by size:
3599 1352 708 297
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 2635 1184
Size: 1451 4505
BstXI CCAn_nnnn'nTGG
Cuts at: 2227 2783 2227
Size: 556 5400
BstYI r'GATC_y
Cuts at: 1972 3286 3653 4038 4055 5507 5593 5605 5951
Size: 1314 367 385 17 1452 86 12 346
Cuts at: 5951 1972
Size: 1977
Fragments arranged by size:
1977 1452 1314 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2640 3028 513
Size: 552 939 636 388 3441
Fragments arranged by size:
3441 939 636 552 388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2074 487
Size: 683 56 848 4369
Fragments arranged by size:
4369 848 683 56
BtsI GCAGTG_nn'
Cuts at: 2468 3472 4266 4286 2468
Size: 1004 794 20 4138
Fragments arranged by size:
4138 1004 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2878 2913 529
Size: 35 2314 35 3572
Fragments arranged by size:
3572 2314 35 35
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2899 3403 4292
Size: 54 1425 50 275 18 1069 504 889
Cuts at: 4292 5921 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1069 889 504 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2801 3886 1353
Size: 1448 1085 3423
Fragments arranged by size:
3423 1448 1085
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2115 3178 4543 4917 5063 387
Size: 1064 138 526 1063 1365 374 146 1280
Fragments arranged by size:
1365 1280 1064 1063 526 374 146 138
EcoICRI GAG'CTC
Cuts at: 3085 3085
Size: 5956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2538 2582 2594 2984 3215 4019
Size: 393 413 493 44 12 390 231 804
Cuts at: 4019 4616 5403 5671 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 804 787 597 493 413 393 390
268 231 44 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2564 2564
Size: 5956
EcoO109I rG'GnC_Cy
Cuts at: 2345 2618 2650 3093 2345
Size: 273 32 443 5208
Fragments arranged by size:
5208 443 273 32
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 2017 2024 2112 2173 2824 5103 1678
Size: 339 7 88 61 651 2279 2531
Fragments arranged by size:
2531 2279 651 339 88 61 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2476 4597 4629 4671
Size: 32 135 32 872 32 2121 32 42
Cuts at: 4671 4703 1373
Size: 32 2626
Fragments arranged by size:
2626 2121 872 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2068 3300 3546 669
Size: 988 281 130 1232 246 3079
Fragments arranged by size:
3079 1232 988 281 246 130
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KasI G'GCGC_C
Cuts at: 2013 2020 2108 2013
Size: 7 88 5861
Fragments arranged by size:
5861 88 7
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2892 5070 5254 1305
Size: 1587 2178 184 2007
Fragments arranged by size:
2178 2007 1587 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2901 10
Size: 54 1425 343 1069 3065
Fragments arranged by size:
3065 1425 1069 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2196 3038 3914 4273 4432 518
Size: 648 1030 842 876 359 159 2042
Fragments arranged by size:
2042 1030 876 842 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2683 2695 3142 3372 4049 5197 5442 1606
Size: 1077 12 447 230 677 1148 245 2120
Fragments arranged by size:
2120 1148 1077 677 447 245 230 12
NaeI GCC'GGC
Cuts at: 2012 2012
Size: 5956
NarI GG'CG_CC
Cuts at: 2014 2021 2109 2014
Size: 7 88 5861
Fragments arranged by size:
5861 88 7
NcoI C'CATG_G
Cuts at: 513 1065 2004 3028 513
Size: 552 939 1024 3441
Fragments arranged by size:
3441 1024 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NgoMIV G'CCGG_C
Cuts at: 2010 2010
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 3314 4859 1132
Size: 97 2085 1545 2229
Fragments arranged by size:
2229 2085 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5956
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2650 3093 2650
Size: 443 5513
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PstI C_TGCA'G
Cuts at: 830 2151 2257 2475 2756 3308 830
Size: 1321 106 218 281 552 3478
Fragments arranged by size:
3478 1321 552 281 218 106
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
PvuII CAG'CTG
Cuts at: 2683 2695 3142 2683
Size: 12 447 5497
Fragments arranged by size:
5497 447 12
SacI G_AGCT'C
Cuts at: 3087 3087
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 2066 3298 1936
Size: 130 1232 4594
Fragments arranged by size:
4594 1232 130
SanDI GG'GwC_CC
Cuts at: 3093 3093
Size: 5956
SbfI CC_TGCA'GG
Cuts at: 2756 3308 2756
Size: 552 5404
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SfcI C'TryA_G
Cuts at: 826 1045 2147 2253 2471 2752 3304 4439 5120
Size: 219 1102 106 218 281 552 1135 681
Cuts at: 5120 5311 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1102 681 552 281 219 218
191 106
SfoI GGC'GCC
Cuts at: 2015 2022 2110 2015
Size: 7 88 5861
Fragments arranged by size:
5861 88 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2173 2654 4060 4961 5223 820
Size: 197 926 230 481 1406 901 262 1553
Fragments arranged by size:
1553 1406 926 901 481 262 230 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 2660 2750 2952 1517
Size: 1143 90 202 4521
Fragments arranged by size:
4521 1143 202 90
StyI C'CwwG_G
Cuts at: 513 1065 2004 2334 2947 3028 3088 3395 513
Size: 552 939 330 613 81 60 307 3074
Fragments arranged by size:
3074 939 613 552 330 307 81 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3983 4000 4153 4338 4783 325
Size: 1255 2403 17 153 185 445 1498
Fragments arranged by size:
2403 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4202 5914 96
Size: 274 80 33 51 1443 2225 1712 138
Fragments arranged by size:
2225 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5916 5948 5916
Size: 32 5924
Tth111I GACn'n_nGTC
Cuts at: 1316 2890 1316
Size: 1574 4382
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2224 2224
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI
SwaI