Restriction map for
pFN21AB5406
(Circular) (Six-base) MAPSORT of: pFN21AB5406.seq Check: 8469 from: 1 to: 5698
pFN21AB5406 5698 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2161 2161
Size: 5698
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2090 3041 1937
Size: 153 951 4594
Fragments arranged by size:
4594 951 153
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2007 2543 3761 5145 5413 1632
Size: 375 536 1218 1384 268 1917
Fragments arranged by size:
1917 1384 1218 536 375 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 4597 5574 1203
Size: 3394 977 1327
Fragments arranged by size:
3394 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AlwNI CAG_nnn'CTG
Cuts at: 2968 3441 5013 2968
Size: 473 1572 3653
Fragments arranged by size:
3653 1572 473
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 3007 3233 3385 5494 1493
Size: 1514 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2438 4252 160
Size: 872 1406 1814 1606
Fragments arranged by size:
1814 1606 1406 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 3023 1870
Size: 73 1080 4545
Fragments arranged by size:
4545 1080 73
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2600 3019 4474 618
Size: 325 217 627 813 419 1455 1842
Fragments arranged by size:
1842 1455 813 627 419 325 217
BanII G_rGCy'C
Cuts at: 1391 3017 1391
Size: 1626 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4727
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4309 136
Size: 107 122 71 631 605 2637 1525
Fragments arranged by size:
2637 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2319 5693 2319
Size: 3374 2324
BlpI GC'TnA_GC
Cuts at: 3115 3115
Size: 5698
Bme1580I G_kGCm'C
Cuts at: 1790 3759 4915 1790
Size: 1969 1156 2573
Fragments arranged by size:
2573 1969 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3429 4387 442
Size: 416 53 83 399 24 2012 958 1753
Fragments arranged by size:
2012 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 2879 4358 1239
Size: 1115 525 1479 2579
Fragments arranged by size:
2579 1479 1115 525
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2043 2819 3823 4688 4986 2043
Size: 776 1004 865 298 2755
Fragments arranged by size:
2755 1004 865 776 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2057 2855 4361 882
Size: 617 558 798 1506 2219
Fragments arranged by size:
2219 1506 798 617 558
BsaAI yAC'GTr
Cuts at: 493 1204 3489 493
Size: 711 2285 2702
Fragments arranged by size:
2702 2285 711
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3505 3887 4522 275
Size: 53 83 186 936 1972 382 635 1451
Fragments arranged by size:
1972 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4131 4529 4803 4950 5361 4131
Size: 398 274 147 411 4468
Fragments arranged by size:
4468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2711 2741 1178
Size: 30 165 30 1308 30 4135
Fragments arranged by size:
4135 1308 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5698
BseYI C'CCAG_C
Cuts at: 2035 4901 5397 2035
Size: 2866 496 2336
Fragments arranged by size:
2866 2336 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2273 1163
Size: 573 537 4588
Fragments arranged by size:
4588 573 537
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2976 3759 3844 4915 2976
Size: 783 85 1071 3759
Fragments arranged by size:
3759 1071 783 85
BsmI GAATG_Cn'
Cuts at: 2517 3209 3302 2517
Size: 692 93 4913
Fragments arranged by size:
4913 692 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 2003
Size: 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2976 3017 3759 3844 4915 1391
Size: 399 1186 41 742 85 1071 2174
Fragments arranged by size:
2174 1186 1071 742 399 85 41
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 2100 3587 5317 2100
Size: 1487 1730 2481
Fragments arranged by size:
2481 1730 1487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2161 2853 3053 844
Size: 1317 692 200 3489
Fragments arranged by size:
3489 1317 692 200
BsrBI CCG'CTC
Cuts at: 1394 1823 3585 1394
Size: 429 1762 3507
Fragments arranged by size:
3507 1762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4193 4367 65
Size: 1183 2945 174 1396
Fragments arranged by size:
2945 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4342 4529 1700
Size: 110 2532 187 2869
Fragments arranged by size:
2869 2532 187 110
BsrGI T'GTAC_A
Cuts at: 96 2496 2665 96
Size: 2400 169 3129
Fragments arranged by size:
3129 2400 169
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5698
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstXI CCAn_nnnn'nTGG
Cuts at: 2581 2581
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 2319 2451 3028 3395 3780 3797 5249 5335
Size: 347 132 577 367 385 17 1452 86
Cuts at: 5335 5347 5693 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 577 385 367 347 346 132
86 17 12
BstZ17I GTA'TAC
Cuts at: 2091 2091
Size: 5698
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4207
Fragments arranged by size:
4207 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2096 487
Size: 683 56 870 4089
Fragments arranged by size:
4089 870 683 56
BtsI GCAGTG_nn'
Cuts at: 3214 4008 4028 3214
Size: 794 20 4884
Fragments arranged by size:
4884 794 20
ClaI AT'CG_AT
Cuts at: 1554 2809 1554
Size: 1255 4443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 2383 3002 2383
Size: 619 5079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3145 4034 5663
Size: 54 1425 50 275 18 1315 889 1629
Cuts at: 5663 8
Size: 43
Fragments arranged by size:
1629 1425 1315 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2437 2580 3628 1353
Size: 1084 143 1048 3423
Fragments arranged by size:
3423 1084 1048 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4285 4659 4805 387
Size: 1064 138 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2354 2543 2879 3761 4358 5145
Size: 393 375 347 189 336 882 597 787
Cuts at: 5145 5413 1239
Size: 268 1524
Fragments arranged by size:
1524 882 787 597 393 375 347 336
268 189
EcoO109I rG'GnC_Cy
Cuts at: 2965 2965
Size: 5698
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 4845 1678
Size: 3167 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2414 2446 2465 2497 2555
Size: 32 135 32 842 32 19 32 58
Cuts at: 2555 2587 2711 2743 4339 4371 4413 4445 1373
Size: 32 124 32 1596 32 42 32 2626
Fragments arranged by size:
2626 1596 842 135 124 58 42 32
32 32 32 32 32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3288 669
Size: 988 281 1104 246 3079
Fragments arranged by size:
3079 1104 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2181 748
Size: 1433 4265
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2100 4812 4996 1305
Size: 795 2712 184 2007
Fragments arranged by size:
2712 2007 795 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3876
Fragments arranged by size:
3876 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3656 4015 4174 518
Size: 648 2490 359 159 2042
Fragments arranged by size:
2490 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 3114 3791 4939 5184 1606
Size: 433 1075 677 1148 245 2120
Fragments arranged by size:
2120 1148 1075 677 433 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4207
Fragments arranged by size:
4207 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 2260 3056 4601 1132
Size: 97 1031 796 1545 2229
Fragments arranged by size:
2229 1545 1031 796 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5698
PfoI T'CCnGG_A
Cuts at: 2370 2370
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PstI C_TGCA'G
Cuts at: 830 2172 2964 3050 830
Size: 1342 792 86 3478
Fragments arranged by size:
3478 1342 792 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
PvuII CAG'CTG
Cuts at: 2039 2039
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SapI GCTCTTCn'nnn_
Cuts at: 2580 2580
Size: 5698
SbfI CC_TGCA'GG
Cuts at: 2172 2964 3050 2172
Size: 792 86 4820
Fragments arranged by size:
4820 792 86
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SfcI C'TryA_G
Cuts at: 826 1045 2168 2591 2960 3046 4181 4862 5053
Size: 219 1123 423 369 86 1135 681 191
Cuts at: 5053 826
Size: 1471
Fragments arranged by size:
1471 1135 1123 681 423 369 219 191
86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2058 2834 3802 4703 4965 820
Size: 197 926 115 776 968 901 262 1553
Fragments arranged by size:
1553 968 926 901 776 262 197 115
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 2260 3056 1132
Size: 1128 796 3774
Fragments arranged by size:
3774 1128 796
SspI AAT'ATT
Cuts at: 5 52 2614 3622 5
Size: 47 2562 1008 2081
Fragments arranged by size:
2562 2081 1008 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2004 2310 3137 513
Size: 552 939 306 827 3074
Fragments arranged by size:
3074 939 827 552 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2522 3725 3742 3895 4080 4525 325
Size: 1255 942 1203 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1203 942 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2496 2665 3944
Size: 274 80 33 51 1443 519 169 1279
Cuts at: 3944 5656 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1279 519 274 169 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5658 5690 5658
Size: 32 5666
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2617 2617
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI