Restriction map for
pFN21AB5403
(Circular) (Six-base) MAPSORT of: pFN21AB5403.seq Check: 7727 from: 1 to: 7444
pFN21AB5403 7444 bp
With 182 enzymes: SgfI *
October 20, 2009 10:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4035 4035
Size: 7444
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5254 278
Size: 53 83 186 936 3718 2468
Fragments arranged by size:
3718 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4059 4787 1937
Size: 2122 728 4594
Fragments arranged by size:
4594 2122 728
Acc65I G'GTAC_C
Cuts at: 3750 4765 3750
Size: 1015 6429
AclI AA'CG_TT
Cuts at: 1563 5571 5944 1563
Size: 4008 373 3063
Fragments arranged by size:
4008 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2532 5507 6891 7159 1632
Size: 900 2975 1384 268 1917
Fragments arranged by size:
2975 1917 1384 900 268
AfeI AGC'GCT
Cuts at: 3467 3467
Size: 7444
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7247
AflIII A'CryG_T
Cuts at: 1203 3701 4210 4431 6343 7320 1203
Size: 2498 509 221 1912 977 1327
Fragments arranged by size:
2498 1912 1327 977 509 221
AgeI A'CCGG_T
Cuts at: 2024 6275 2024
Size: 4251 3193
AhdI GACnn_n'nnGTC
Cuts at: 2038 3913 6173 2038
Size: 1875 2260 3309
Fragments arranged by size:
3309 2260 1875
AleI CACnn'nnGTG
Cuts at: 1166 3842 1166
Size: 2676 4768
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3530 3860 1184
Size: 2346 330 4768
Fragments arranged by size:
4768 2346 330
AlwNI CAG_nnn'CTG
Cuts at: 3316 3952 4180 4567 5187 6759 3316
Size: 636 228 387 620 1572 4001
Fragments arranged by size:
4001 1572 636 620 387 228
ApaLI G'TGCA_C
Cuts at: 4457 5501 6657 4457
Size: 1044 1156 5244
Fragments arranged by size:
5244 1156 1044
ApoI r'AATT_y
Cuts at: 1493 3365 4753 4979 5131 7240 1493
Size: 1872 1388 226 152 2109 1697
Fragments arranged by size:
2109 1872 1697 1388 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4485 5998 160
Size: 872 3453 1513 1606
Fragments arranged by size:
3453 1606 1513 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
AvaI C'yCGr_G
Cuts at: 1870 1943 2492 4313 4769 1870
Size: 73 549 1821 456 4545
Fragments arranged by size:
4545 1821 549 456 73
BamHI G'GATC_C
Cuts at: 3693 4774 5141 3693
Size: 1081 367 5996
Fragments arranged by size:
5996 1081 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2782 3750 4005 4640 4765
Size: 325 217 627 995 968 255 635 125
Cuts at: 4765 6220 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 995 968 635 627 325 255
217 125
BanII G_rGCy'C
Cuts at: 1391 2070 2259 3590 4551 4763 1391
Size: 679 189 1331 961 212 4072
Fragments arranged by size:
4072 1331 961 679 212 189
BbeI G_GCGC'C
Cuts at: 4009 4009
Size: 7444
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2697 4259 928
Size: 971 798 1562 4113
Fragments arranged by size:
4113 1562 971 798
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2483 2517 5635 5669 1903
Size: 34 546 34 3118 34 3678
Fragments arranged by size:
3678 3118 546 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5336 6546 5336
Size: 1210 6234
BclI T'GATC_A
Cuts at: 1548 4131 4479 1548
Size: 2583 348 4513
Fragments arranged by size:
4513 2583 348
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4229 4710 6055
Size: 107 122 71 631 605 2557 481 1345
Cuts at: 6055 136
Size: 1525
Fragments arranged by size:
2557 1525 1345 631 605 481 122 107
71
BglII A'GATC_T
Cuts at: 7439 7439
Size: 7444
BlpI GC'TnA_GC
Cuts at: 2117 4861 2117
Size: 2744 4700
Bme1580I G_kGCm'C
Cuts at: 1790 3488 4461 4643 5505 6661 1790
Size: 1698 973 182 862 1156 2573
Fragments arranged by size:
2573 1698 1156 973 862 182
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7444
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2704 5175 6133
Size: 416 53 83 399 24 1287 2471 958
Cuts at: 6133 442
Size: 1753
Fragments arranged by size:
2471 1753 1287 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7444
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3607 3607
Size: 7444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3494 4061 4070 4103 6104 1239
Size: 2255 567 9 33 2001 2579
Fragments arranged by size:
2579 2255 2001 567 33 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2749 5569 6434 6732 2749
Size: 2820 865 298 3461
Fragments arranged by size:
3461 2820 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4656 6107 882
Size: 617 3157 1451 2219
Fragments arranged by size:
3157 2219 1451 617
BsaAI yAC'GTr
Cuts at: 493 1204 3412 5235 493
Size: 711 2208 1823 2702
Fragments arranged by size:
2702 2208 1823 711
BsaBI GATnn'nnATC
Cuts at: 3233 7438 3233
Size: 4205 3239
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3111 4006 5251 5633
Size: 53 83 186 936 1578 895 1245 382
Cuts at: 5633 6268 275
Size: 635 1451
Fragments arranged by size:
1578 1451 1245 936 895 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2024 2196 3690 5877 6275 6549 6696 7107 2024
Size: 172 1494 2187 398 274 147 411 2361
Fragments arranged by size:
2361 2187 1494 411 398 274 172 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3096 3126 3383 3413 3641
Size: 30 165 30 1693 30 257 30 228
Cuts at: 3641 3671 4409 4439 1178
Size: 30 738 30 4183
Fragments arranged by size:
4183 1693 738 257 228 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2192 2543 2747 2891 2894 2897 3491
Size: 892 114 351 204 144 3 3 594
Cuts at: 3491 3509 3580 4505 1186
Size: 18 71 925 4125
Fragments arranged by size:
4125 925 892 594 351 204 144 114
71 18 3 3
BseYI C'CCAG_C
Cuts at: 3850 4183 4557 4591 6647 7143 3850
Size: 333 374 34 2056 496 4151
Fragments arranged by size:
4151 2056 496 374 333 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3281 3482 3512 3722 3839 3866 4013
Size: 573 1545 201 30 210 117 27 147
Cuts at: 4013 1163
Size: 4594
Fragments arranged by size:
4594 1545 573 210 201 147 117 30
27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4894 5655 6683 1540
Size: 275 186 2893 761 1028 2301
Fragments arranged by size:
2893 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2259 2423 3420 4461 5505 5590 6661 2070
Size: 189 164 997 1041 1044 85 1071 2853
Fragments arranged by size:
2853 1071 1044 1041 997 189 164 85
BsmI GAATG_Cn'
Cuts at: 4955 5048 4955
Size: 93 7351
BsmBI CGTCTCn'nnnn_
Cuts at: 4412 4412
Size: 7444
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2259 2423 3420 3488 3590 4461
Size: 399 280 189 164 997 68 102 871
Cuts at: 4461 4551 4643 4763 5505 5590 6661 1391
Size: 90 92 120 742 85 1071 2174
Fragments arranged by size:
2174 1071 997 871 742 399 280 189
164 120 102 92 90 85 68
BspEI T'CCGG_A
Cuts at: 2196 7107 2196
Size: 4911 2533
BspHI T'CATG_A
Cuts at: 3774 5333 7063 3774
Size: 1559 1730 4155
Fragments arranged by size:
4155 1730 1559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3303 3522 4035 4799 844
Size: 2459 219 513 764 3489
Fragments arranged by size:
3489 2459 764 513 219
BsrBI CCG'CTC
Cuts at: 1394 1823 4003 5331 1394
Size: 429 2180 1328 3507
Fragments arranged by size:
3507 2180 1328 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5939 6113 65
Size: 1183 4691 174 1396
Fragments arranged by size:
4691 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2024 6088 6275 1700
Size: 110 214 4064 187 2869
Fragments arranged by size:
4064 2869 214 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7444
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7444
BssSI C'ACGA_G
Cuts at: 3021 5504 6516 3021
Size: 2483 1012 3949
Fragments arranged by size:
3949 2483 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7444
BstBI TT'CG_AA
Cuts at: 6248 6248
Size: 7444
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7444
BstXI CCAn_nnnn'nTGG
Cuts at: 2242 2869 3195 3920 4081 4240 4414 2242
Size: 627 326 725 161 159 174 5272
Fragments arranged by size:
5272 725 627 326 174 161 159
BstYI r'GATC_y
Cuts at: 1972 2436 3009 3693 3822 4086 4350 4444 4774
Size: 464 573 684 129 264 264 94 330
Cuts at: 4774 5141 5526 5543 6995 7081 7093 7439 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 684 573 464 385 367 346
330 264 264 129 94 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3582 3582
Size: 7444
BtgI C'CryG_G
Cuts at: 513 1065 3325 3840 4705 513
Size: 552 2260 515 865 3252
Fragments arranged by size:
3252 2260 865 552 515
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3124 4128 487
Size: 683 56 1898 1004 3803
Fragments arranged by size:
3803 1898 1004 683 56
BtsI GCAGTG_nn'
Cuts at: 2161 3314 4399 4551 4960 5754 5774 2161
Size: 1153 1085 152 409 794 20 3831
Fragments arranged by size:
3831 1153 1085 794 409 152 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7444
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7409
DraI TTT'AAA
Cuts at: 4748 4748
Size: 7444
DraIII CAC_nnn'GTG
Cuts at: 2238 3320 2238
Size: 1082 6362
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6451 809
Size: 507 5135 1802
Fragments arranged by size:
5135 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3922 4098 4581
Size: 54 1425 50 275 18 2092 176 483
Cuts at: 4581 4891 5780 7409 8
Size: 310 889 1629 43
Fragments arranged by size:
2092 1629 1425 889 483 310 275 176
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4891 1537
Size: 275 3079 4090
Fragments arranged by size:
4090 3079 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2241 2551 3276 4353 4385 5374 1353
Size: 888 310 725 1077 32 989 3423
Fragments arranged by size:
3423 1077 989 888 725 310 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2937 3857 6031 6405 6551 387
Size: 1064 138 1348 920 2174 374 146 1280
Fragments arranged by size:
2174 1348 1280 1064 920 374 146 138
EcoICRI GAG'CTC
Cuts at: 2068 2257 2068
Size: 189 7255
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2532 3494 4061 4070 4103 5507 6104
Size: 393 900 962 567 9 33 1404 597
Cuts at: 6104 6891 7159 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1404 962 900 787 597 567 393
268 33 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2085 2873 3104 3881 2085
Size: 788 231 777 5648
Fragments arranged by size:
5648 788 777 231
EcoO109I rG'GnC_Cy
Cuts at: 3949 4015 4187 4303 4371 3949
Size: 66 172 116 68 7022
Fragments arranged by size:
7022 172 116 68 66
EcoRI G'AATT_C
Cuts at: 4753 5131 4753
Size: 378 7066
EcoRV GAT'ATC
Cuts at: 3235 3235
Size: 7444
FspI TGC'GCA
Cuts at: 3521 3962 5950 3521
Size: 441 1988 5015
Fragments arranged by size:
5015 1988 441
HaeII r_GCGC'y
Cuts at: 1678 2869 3469 4009 6591 1678
Size: 1191 600 540 2582 2531
Fragments arranged by size:
2582 2531 1191 600 540
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2030 2056 2062 2447
Size: 32 135 32 452 6 26 6 385
Cuts at: 2447 2479 2651 2683 2711 2743 2843 2875 3096
Size: 32 172 32 28 32 100 32 221
Cuts at: 3096 3128 3383 3415 3899 3931 4116 4148 4409
Size: 32 255 32 484 32 185 32 261
Cuts at: 4409 4441 6085 6117 6159 6191 1373
Size: 32 1644 32 42 32 2626
Fragments arranged by size:
2626 1644 484 452 385 261 255 221
185 172 135 100 42 32 32 32
32 32 32 32 32 32 32 32
32 32 28 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4788 5034 669
Size: 988 281 2850 246 3079
Fragments arranged by size:
3079 2850 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7444
HpaI GTT'AAC
Cuts at: 5034 5034
Size: 7444
KasI G'GCGC_C
Cuts at: 4005 4005
Size: 7444
KpnI G_GTAC'C
Cuts at: 3754 4769 3754
Size: 1015 6429
MfeI C'AATT_G
Cuts at: 5043 5043
Size: 7444
MluI A'CGCG_T
Cuts at: 7320 7320
Size: 7444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2065 2748 3244 6558 6742 1305
Size: 760 683 496 3314 184 2007
Fragments arranged by size:
3314 2007 760 683 496 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5622
Fragments arranged by size:
5622 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3773 3842 4238 4412 5402 5761 5920
Size: 648 2607 69 396 174 990 359 159
Cuts at: 5920 518
Size: 2042
Fragments arranged by size:
2607 2042 990 648 396 359 174 159
69
MspA1I CmG'CkG
Cuts at: 1606 3089 3571 3850 4156 4579 4707 4860 5537
Size: 1483 482 279 306 423 128 153 677
Cuts at: 5537 6685 6930 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1483 1148 677 482 423 306 279
245 153 128
NarI GG'CG_CC
Cuts at: 4006 4006
Size: 7444
NcoI C'CATG_G
Cuts at: 513 1065 3840 513
Size: 552 2775 4117
Fragments arranged by size:
4117 2775 552
NdeI CA'TA_TG
Cuts at: 387 2272 387
Size: 1885 5559
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7444
NotI GC'GGCC_GC
Cuts at: 4891 4891
Size: 7444
NspI r_CATG'y
Cuts at: 1132 1229 3705 3806 4214 4435 4702 4802 6347
Size: 97 2476 101 408 221 267 100 1545
Cuts at: 6347 1132
Size: 2229
Fragments arranged by size:
2476 2229 1545 408 267 221 101 100
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7444
PciI A'CATG_T
Cuts at: 3701 4210 4431 6343 3701
Size: 509 221 1912 4802
Fragments arranged by size:
4802 1912 509 221
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7444
PfoI T'CCnGG_A
Cuts at: 4080 4353 4080
Size: 273 7171
PmeI GTTT'AAAC
Cuts at: 4748 4748
Size: 7444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5513 5545 7026 7058 5513
Size: 32 1481 32 5899
Fragments arranged by size:
5899 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4015 4187 4303 4371 4015
Size: 172 116 68 7088
Fragments arranged by size:
7088 172 116 68
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7444
PsiI TTA'TAA
Cuts at: 5014 5014
Size: 7444
PstI C_TGCA'G
Cuts at: 830 3972 4578 4796 830
Size: 3142 606 218 3478
Fragments arranged by size:
3478 3142 606 218
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7444
PvuII CAG'CTG
Cuts at: 3571 3850 4156 4579 3571
Size: 279 306 423 6436
Fragments arranged by size:
6436 423 306 279
SacI G_AGCT'C
Cuts at: 2070 2259 2070
Size: 189 7255
SacII CC_GC'GG
Cuts at: 4708 4708
Size: 7444
SalI G'TCGA_C
Cuts at: 1936 4786 1936
Size: 2850 4594
SanDI GG'GwC_CC
Cuts at: 4187 4187
Size: 7444
SapI GCTCTTCn'nnn_
Cuts at: 2551 2551
Size: 7444
SbfI CC_TGCA'GG
Cuts at: 3972 4796 3972
Size: 824 6620
ScaI AGT'ACT
Cuts at: 5692 7404 5692
Size: 1712 5732
SexAI A'CCwGG_T
Cuts at: 4062 4062
Size: 7444
SfcI C'TryA_G
Cuts at: 826 1045 3014 3968 4387 4574 4792 5927 6608
Size: 219 1969 954 419 187 218 1135 681
Cuts at: 6608 6799 826
Size: 191 1471
Fragments arranged by size:
1969 1471 1135 954 681 419 219 218
191 187
SfoI GGC'GCC
Cuts at: 4007 4007
Size: 7444
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7444
SmaI CCC'GGG
Cuts at: 1872 4771 1872
Size: 2899 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2764 5548 6449 6711 820
Size: 197 926 821 2784 901 262 1553
Fragments arranged by size:
2784 1553 926 901 821 262 197
SnaBI TAC'GTA
Cuts at: 493 5235 493
Size: 4742 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7444
SphI G_CATG'C
Cuts at: 1132 3806 4802 1132
Size: 2674 996 3774
Fragments arranged by size:
3774 2674 996
SspI AAT'ATT
Cuts at: 5 52 3274 5368 5
Size: 47 3222 2094 2081
Fragments arranged by size:
3222 2094 2081 47
StuI AGG'CCT
Cuts at: 1517 3876 1517
Size: 2359 5085
StyI C'CwwG_G
Cuts at: 513 1065 2160 2409 2961 3675 3840 3871 3882
Size: 552 1095 249 552 714 165 31 11
Cuts at: 3882 3930 4227 4883 513
Size: 48 297 656 3074
Fragments arranged by size:
3074 1095 714 656 552 552 297 249
165 48 31 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2800 2993 4232 5471 5488 5641 5826
Size: 1255 1220 193 1239 1239 17 153 185
Cuts at: 5826 6271 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1239 1239 1220 445 193 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3663 5690 7402
Size: 274 80 33 51 1443 1686 2027 1712
Cuts at: 7402 96
Size: 138
Fragments arranged by size:
2027 1712 1686 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3306 3338 3641 3673 7404 7436 3306
Size: 32 303 32 3731 32 3314
Fragments arranged by size:
3731 3314 303 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2573 4325 4423 1316
Size: 1257 1752 98 4337
Fragments arranged by size:
4337 1752 1257 98
XbaI T'CTAG_A
Cuts at: 4780 4780
Size: 7444
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2290 3847 3937 2290
Size: 1557 90 5797
Fragments arranged by size:
5797 1557 90
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7444
XmaI C'CCGG_G
Cuts at: 1870 4769 1870
Size: 2899 4545
XmnI GAAnn'nnTTC
Cuts at: 5573 5573
Size: 7444
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5252 276
Size: 53 83 186 936 3718 2468
Fragments arranged by size:
3718 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SfiI
SrfI SwaI