Restriction map for
pFN21AB5399
(Circular) (Six-base) MAPSORT of: pFN21AB5399.seq Check: 3756 from: 1 to: 5278
pFN21AB5399 5278 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3088 278
Size: 53 83 186 936 1552 2468
Fragments arranged by size:
2468 1552 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2457 2621 1937
Size: 520 164 4594
Fragments arranged by size:
4594 520 164
Acc65I G'GTAC_C
Cuts at: 2599 2599
Size: 5278
AclI AA'CG_TT
Cuts at: 1563 3405 3778 1563
Size: 1842 373 3063
Fragments arranged by size:
3063 1842 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2055 3341 4725 4993 1632
Size: 423 1286 1384 268 1917
Fragments arranged by size:
1917 1384 1286 423 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5081
AflIII A'CryG_T
Cuts at: 1203 4177 5154 1203
Size: 2974 977 1327
Fragments arranged by size:
2974 1327 977
AgeI A'CCGG_T
Cuts at: 4109 4109
Size: 5278
AhdI GACnn_n'nnGTC
Cuts at: 4007 4007
Size: 5278
AleI CACnn'nnGTG
Cuts at: 1166 2357 1166
Size: 1191 4087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5278
AlwNI CAG_nnn'CTG
Cuts at: 2305 3021 4593 2305
Size: 716 1572 2990
Fragments arranged by size:
2990 1572 716
ApaLI G'TGCA_C
Cuts at: 3335 4491 3335
Size: 1156 4122
ApoI r'AATT_y
Cuts at: 1493 2144 2513 2587 2813 2965 5074 1493
Size: 651 369 74 226 152 2109 1697
Fragments arranged by size:
2109 1697 651 369 226 152 74
AseI AT'TA_AT
Cuts at: 160 1032 3832 160
Size: 872 2800 1606
Fragments arranged by size:
2800 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5278
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 2355 2603 1870
Size: 73 195 217 248 4545
Fragments arranged by size:
4545 248 217 195 73
BamHI G'GATC_C
Cuts at: 2608 2975 2608
Size: 367 4911
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2237 2599 4054 618
Size: 325 217 627 450 362 1455 1842
Fragments arranged by size:
1842 1455 627 450 362 325 217
BanII G_rGCy'C
Cuts at: 1391 2597 1391
Size: 1206 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4307
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3469 3503 1903
Size: 34 1532 34 3678
Fragments arranged by size:
3678 1532 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3170 4380 3170
Size: 1210 4068
BclI T'GATC_A
Cuts at: 1548 2409 1548
Size: 861 4417
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3889 136
Size: 107 122 71 631 605 2217 1525
Fragments arranged by size:
2217 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5273 5273
Size: 5278
BlpI GC'TnA_GC
Cuts at: 2695 2695
Size: 5278
Bme1580I G_kGCm'C
Cuts at: 1790 3339 4495 1790
Size: 1549 1156 2573
Fragments arranged by size:
2573 1549 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5278
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2320 3009 3967
Size: 416 53 83 399 24 903 689 958
Cuts at: 3967 442
Size: 1753
Fragments arranged by size:
1753 958 903 689 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5278
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2266 2390 3938 1239
Size: 1027 124 1548 2579
Fragments arranged by size:
2579 1548 1027 124
Bpu10I CC'TnA_GC
Cuts at: 2445 2445
Size: 5278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2069 2093 3403 4268 4566 2069
Size: 24 1310 865 298 2781
Fragments arranged by size:
2781 1310 865 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3941 882
Size: 617 2442 2219
Fragments arranged by size:
2442 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3069 493
Size: 711 1865 2702
Fragments arranged by size:
2702 1865 711
BsaBI GATnn'nnATC
Cuts at: 5272 5272
Size: 5278
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2184 2441 3085 3467
Size: 53 83 186 936 651 257 644 382
Cuts at: 3467 4102 275
Size: 635 1451
Fragments arranged by size:
1451 936 651 644 635 382 257 186
83 53
BsaWI w'CCGG_w
Cuts at: 3711 4109 4383 4530 4941 3711
Size: 398 274 147 411 4048
Fragments arranged by size:
4048 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5053
Fragments arranged by size:
5053 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5278
BseYI C'CCAG_C
Cuts at: 2199 4481 4977 2199
Size: 2282 496 2500
Fragments arranged by size:
2500 2282 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4705
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2728 3489 4517 1540
Size: 275 186 727 761 1028 2301
Fragments arranged by size:
2301 1028 761 727 275 186
BsiHKAI G_wGCw'C
Cuts at: 2063 3339 3424 4495 2063
Size: 1276 85 1071 2846
Fragments arranged by size:
2846 1276 1071 85
BsmI GAATG_Cn'
Cuts at: 2789 2882 2789
Size: 93 5185
BsmBI CGTCTCn'nnnn_
Cuts at: 2175 2175
Size: 5278
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2597 3339 3424 4495 1391
Size: 399 273 534 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 534 399 273 85
BspEI T'CCGG_A
Cuts at: 4941 4941
Size: 5278
BspHI T'CATG_A
Cuts at: 2412 3167 4897 2412
Size: 755 1730 2793
Fragments arranged by size:
2793 1730 755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2633 844
Size: 1789 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3165 1394
Size: 429 1342 3507
Fragments arranged by size:
3507 1342 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3773 3947 65
Size: 1183 2525 174 1396
Fragments arranged by size:
2525 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3922 4109 1700
Size: 110 2112 187 2869
Fragments arranged by size:
2869 2112 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5278
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5278
BssSI C'ACGA_G
Cuts at: 3338 4350 3338
Size: 1012 4266
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5278
BstBI TT'CG_AA
Cuts at: 4082 4082
Size: 5278
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5278
BstYI r'GATC_y
Cuts at: 1972 2608 2975 3360 3377 4829 4915 4927 5273
Size: 636 367 385 17 1452 86 12 346
Cuts at: 5273 1972
Size: 1977
Fragments arranged by size:
1977 1452 636 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3787
Fragments arranged by size:
3787 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4539
Fragments arranged by size:
4539 683 56
BtsI GCAGTG_nn'
Cuts at: 2096 2567 2794 3588 3608 2096
Size: 471 227 794 20 3766
Fragments arranged by size:
3766 794 471 227 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5278
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5243
DraI TTT'AAA
Cuts at: 2582 2582
Size: 5278
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4285 809
Size: 507 2969 1802
Fragments arranged by size:
2969 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2725 3614 5243
Size: 54 1425 50 275 18 895 889 1629
Cuts at: 5243 8
Size: 43
Fragments arranged by size:
1629 1425 895 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2725 1537
Size: 275 913 4090
Fragments arranged by size:
4090 913 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 3208 1353
Size: 698 1157 3423
Fragments arranged by size:
3423 1157 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3865 4239 4385 387
Size: 1064 138 2276 374 146 1280
Fragments arranged by size:
2276 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2266 2390 3341 3938 4725 4993
Size: 393 423 211 124 951 597 787 268
Cuts at: 4993 1239
Size: 1524
Fragments arranged by size:
1524 951 787 597 423 393 268 211
124
EcoO109I rG'GnC_Cy
Cuts at: 2047 2047
Size: 5278
EcoRI G'AATT_C
Cuts at: 2513 2587 2965 2513
Size: 74 378 4826
Fragments arranged by size:
4826 378 74
FspI TGC'GCA
Cuts at: 3784 3784
Size: 5278
HaeII r_GCGC'y
Cuts at: 1678 4425 1678
Size: 2747 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2119 2151 2244 2276 3919
Size: 32 135 32 547 32 93 32 1643
Cuts at: 3919 3951 3993 4025 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1643 547 135 93 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2622 2868 669
Size: 988 281 684 246 3079
Fragments arranged by size:
3079 988 684 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5278
HpaI GTT'AAC
Cuts at: 2868 2868
Size: 5278
KpnI G_GTAC'C
Cuts at: 2603 2603
Size: 5278
MfeI C'AATT_G
Cuts at: 2877 2877
Size: 5278
MluI A'CGCG_T
Cuts at: 5154 5154
Size: 5278
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4392 4576 1305
Size: 3087 184 2007
Fragments arranged by size:
3087 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3456
Fragments arranged by size:
3456 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 3236 3595 3754 518
Size: 648 1191 879 359 159 2042
Fragments arranged by size:
2042 1191 879 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2694 3371 4519 4764 1606
Size: 1088 677 1148 245 2120
Fragments arranged by size:
2120 1148 1088 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3787
Fragments arranged by size:
3787 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5278
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5278
NotI GC'GGCC_GC
Cuts at: 2725 2725
Size: 5278
NspI r_CATG'y
Cuts at: 1132 1229 2207 2636 4181 1132
Size: 97 978 429 1545 2229
Fragments arranged by size:
2229 1545 978 429 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5278
PciI A'CATG_T
Cuts at: 4177 4177
Size: 5278
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5278
PmeI GTTT'AAAC
Cuts at: 2582 2582
Size: 5278
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3347 3379 4860 4892 3347
Size: 32 1481 32 3733
Fragments arranged by size:
3733 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2047
Size: 5278
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5278
PsiI TTA'TAA
Cuts at: 2848 2848
Size: 5278
PstI C_TGCA'G
Cuts at: 830 2562 2630 830
Size: 1732 68 3478
Fragments arranged by size:
3478 1732 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5278
SalI G'TCGA_C
Cuts at: 1936 2620 1936
Size: 684 4594
SapI GCTCTTCn'nnn_
Cuts at: 2051 2051
Size: 5278
SbfI CC_TGCA'GG
Cuts at: 2630 2630
Size: 5278
ScaI AGT'ACT
Cuts at: 3526 5238 3526
Size: 1712 3566
SexAI A'CCwGG_T
Cuts at: 2049 2049
Size: 5278
SfcI C'TryA_G
Cuts at: 826 1045 2558 2626 3761 4442 4633 826
Size: 219 1513 68 1135 681 191 1471
Fragments arranged by size:
1513 1471 1135 681 219 191 68
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5278
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5278
SmaI CCC'GGG
Cuts at: 1872 2605 1872
Size: 733 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2084 2108 2138 3382 4283 4545
Size: 197 926 141 24 30 1244 901 262
Cuts at: 4545 820
Size: 1553
Fragments arranged by size:
1553 1244 926 901 262 197 141 30
24
SnaBI TAC'GTA
Cuts at: 493 3069 493
Size: 2576 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5278
SphI G_CATG'C
Cuts at: 1132 2207 2636 1132
Size: 1075 429 3774
Fragments arranged by size:
3774 1075 429
SspI AAT'ATT
Cuts at: 5 52 3202 5
Size: 47 3150 2081
Fragments arranged by size:
3150 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5278
StyI C'CwwG_G
Cuts at: 513 1065 2004 2717 513
Size: 552 939 713 3074
Fragments arranged by size:
3074 939 713 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3305 3322 3475 3660 4105 325
Size: 1255 1725 17 153 185 445 1498
Fragments arranged by size:
1725 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3524 5236 96
Size: 274 80 33 51 1443 1547 1712 138
Fragments arranged by size:
1712 1547 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5238 5270 5238
Size: 32 5246
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5278
XbaI T'CTAG_A
Cuts at: 2614 2614
Size: 5278
XhoI C'TCGA_G
Cuts at: 1943 2138 1943
Size: 195 5083
XmaI C'CCGG_G
Cuts at: 1870 2603 1870
Size: 733 4545
XmnI GAAnn'nnTTC
Cuts at: 3407 3407
Size: 5278
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3086 276
Size: 53 83 186 936 1552 2468
Fragments arranged by size:
2468 1552 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI XcmI