Restriction map for
pFN21AB5383
(Circular) (Six-base) MAPSORT of: pFN21AB5383.seq Check: 524 from: 1 to: 6322
pFN21AB5383 6322 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4132 278
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3665 1937
Size: 1728 4594
Acc65I G'GTAC_C
Cuts at: 3643 3643
Size: 6322
AclI AA'CG_TT
Cuts at: 1563 4449 4822 1563
Size: 2886 373 3063
Fragments arranged by size:
3063 2886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2092 2360 4385 5769 6037 1632
Size: 460 268 2025 1384 268 1917
Fragments arranged by size:
2025 1917 1384 460 268 268
AfeI AGC'GCT
Cuts at: 3212 3212
Size: 6322
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6125
AflIII A'CryG_T
Cuts at: 1203 2454 5221 6198 1203
Size: 1251 2767 977 1327
Fragments arranged by size:
2767 1327 1251 977
AgeI A'CCGG_T
Cuts at: 5153 5153
Size: 6322
AhdI GACnn_n'nnGTC
Cuts at: 5051 5051
Size: 6322
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6322
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3513 3545 3513
Size: 32 6290
AlwNI CAG_nnn'CTG
Cuts at: 3538 4065 5637 3538
Size: 527 1572 4223
Fragments arranged by size:
4223 1572 527
ApaLI G'TGCA_C
Cuts at: 4379 5535 4379
Size: 1156 5166
ApoI r'AATT_y
Cuts at: 1493 2707 2857 3005 3631 3857 4009 6118 1493
Size: 1214 150 148 626 226 152 2109 1697
Fragments arranged by size:
2109 1697 1214 626 226 152 150 148
AseI AT'TA_AT
Cuts at: 160 1032 3483 4876 160
Size: 872 2451 1393 1606
Fragments arranged by size:
2451 1606 1393 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
AvaI C'yCGr_G
Cuts at: 1870 1943 2009 3618 3647 1870
Size: 73 66 1609 29 4545
Fragments arranged by size:
4545 1609 73 66 29
BamHI G'GATC_C
Cuts at: 3652 4019 3652
Size: 367 5955
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2069 2318 3643 5098
Size: 325 217 627 233 49 249 1325 1455
Cuts at: 5098 618
Size: 1842
Fragments arranged by size:
1842 1455 1325 627 325 249 233 217
49
BanII G_rGCy'C
Cuts at: 1391 3265 3641 1391
Size: 1874 376 4072
Fragments arranged by size:
4072 1874 376
BbeI G_GCGC'C
Cuts at: 2073 2073
Size: 6322
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3003 928
Size: 971 1104 4247
Fragments arranged by size:
4247 1104 971
BbvCI CC'TCA_GC
Cuts at: 3090 3090
Size: 6322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4513 4547 1903
Size: 34 50 34 2492 34 3678
Fragments arranged by size:
3678 2492 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4214 5424 4214
Size: 1210 5112
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6322
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4933 136
Size: 107 122 71 631 605 3261 1525
Fragments arranged by size:
3261 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2614 6317 2614
Size: 3703 2619
BlpI GC'TnA_GC
Cuts at: 3207 3739 3207
Size: 532 5790
Bme1580I G_kGCm'C
Cuts at: 1790 4383 5539 1790
Size: 2593 1156 2573
Fragments arranged by size:
2593 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6322
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4053 5011 442
Size: 416 53 83 399 24 2636 958 1753
Fragments arranged by size:
2636 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3270 4982 1239
Size: 2031 1712 2579
Fragments arranged by size:
2579 2031 1712
Bpu10I CC'TnA_GC
Cuts at: 3090 3090
Size: 6322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2280 3110 4447 5312 5610 2280
Size: 830 1337 865 298 2992
Fragments arranged by size:
2992 1337 865 830 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4985 882
Size: 617 3486 2219
Fragments arranged by size:
3486 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4113 493
Size: 711 2909 2702
Fragments arranged by size:
2909 2702 711
BsaBI GATnn'nnATC
Cuts at: 6316 6316
Size: 6322
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 4129 4511 5146
Size: 53 83 186 936 537 2059 382 635
Cuts at: 5146 275
Size: 1451
Fragments arranged by size:
2059 1451 936 635 537 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4755 5153 5427 5574 5985 4755
Size: 398 274 147 411 5092
Fragments arranged by size:
5092 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6097
Fragments arranged by size:
6097 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3107 1186
Size: 1921 4401
BseYI C'CCAG_C
Cuts at: 2593 3070 5525 6021 2593
Size: 477 2455 496 2894
Fragments arranged by size:
2894 2455 496 477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5749
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2059 3772 4533 5561 1540
Size: 275 186 58 1713 761 1028 2301
Fragments arranged by size:
2301 1713 1028 761 275 186 58
BsiHKAI G_wGCw'C
Cuts at: 3286 3559 4383 4468 5539 3286
Size: 273 824 85 1071 4069
Fragments arranged by size:
4069 1071 824 273 85
BsmI GAATG_Cn'
Cuts at: 2277 3588 3833 3926 2277
Size: 1311 245 93 4673
Fragments arranged by size:
4673 1311 245 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3265 3286 3559 3641 4383 4468 5539
Size: 399 1475 21 273 82 742 85 1071
Cuts at: 5539 1391
Size: 2174
Fragments arranged by size:
2174 1475 1071 742 399 273 85 82
21
BspEI T'CCGG_A
Cuts at: 5985 5985
Size: 6322
BspHI T'CATG_A
Cuts at: 3220 4211 5941 3220
Size: 991 1730 3601
Fragments arranged by size:
3601 1730 991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3677 844
Size: 2833 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2031 4209 1394
Size: 429 208 2178 3507
Fragments arranged by size:
3507 2178 429 208
BsrDI GCAATG_nn'
Cuts at: 65 1248 2337 4817 4991 65
Size: 1183 1089 2480 174 1396
Fragments arranged by size:
2480 1396 1183 1089 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 3410 3450 4966 5153 1700
Size: 110 241 1359 40 1516 187 2869
Fragments arranged by size:
2869 1516 1359 241 187 110 40
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6322
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6322
BssSI C'ACGA_G
Cuts at: 4382 5394 4382
Size: 1012 5310
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6322
BstBI TT'CG_AA
Cuts at: 5126 5126
Size: 6322
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6322
BstXI CCAn_nnnn'nTGG
Cuts at: 2275 3514 2275
Size: 1239 5083
BstYI r'GATC_y
Cuts at: 1972 2100 2614 3652 4019 4404 4421 5873 5959
Size: 128 514 1038 367 385 17 1452 86
Cuts at: 5959 5971 6317 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1038 514 385 367 346 128
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2059 513
Size: 552 994 4776
Fragments arranged by size:
4776 994 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5583
Fragments arranged by size:
5583 683 56
BtsI GCAGTG_nn'
Cuts at: 2580 3838 4632 4652 2580
Size: 1258 794 20 4250
Fragments arranged by size:
4250 1258 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6322
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6287
DraI TTT'AAA
Cuts at: 2856 3105 3626 2856
Size: 249 521 5552
Fragments arranged by size:
5552 521 249
DraIII CAC_nnn'GTG
Cuts at: 3335 3335
Size: 6322
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2455 5329 809
Size: 507 1139 2874 1802
Fragments arranged by size:
2874 1802 1139 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2056 3769 4658
Size: 54 1425 50 275 18 226 1713 889
Cuts at: 4658 6287 8
Size: 1629 43
Fragments arranged by size:
1713 1629 1425 889 275 226 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2056 3769 1537
Size: 275 244 1713 4090
Fragments arranged by size:
4090 1713 275 244
EarI CTCTTCn'nnn_
Cuts at: 1353 2832 3251 4252 1353
Size: 1479 419 1001 3423
Fragments arranged by size:
3423 1479 1001 419
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2110 4909 5283 5429 387
Size: 1064 138 521 2799 374 146 1280
Fragments arranged by size:
2799 1280 1064 521 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2092 2360 3270 4385 4982 5769 6037
Size: 393 460 268 910 1115 597 787 268
Cuts at: 6037 1239
Size: 1524
Fragments arranged by size:
1524 1115 910 787 597 460 393 268
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2429 2429
Size: 6322
EcoO109I rG'GnC_Cy
Cuts at: 2422 2422
Size: 6322
EcoRI G'AATT_C
Cuts at: 3005 3631 4009 3005
Size: 626 378 5318
Fragments arranged by size:
5318 626 378
FspI TGC'GCA
Cuts at: 4828 4828
Size: 6322
HaeII r_GCGC'y
Cuts at: 1678 2073 3214 5469 1678
Size: 395 1141 2255 2531
Fragments arranged by size:
2531 2255 1141 395
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2877 2909 4963 4995 5037
Size: 32 135 32 1305 32 2054 32 42
Cuts at: 5037 5069 1373
Size: 32 2626
Fragments arranged by size:
2626 2054 1305 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3666 3912 669
Size: 988 281 1728 246 3079
Fragments arranged by size:
3079 1728 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2369 2642 748
Size: 1621 273 4428
Fragments arranged by size:
4428 1621 273
HpaI GTT'AAC
Cuts at: 3912 3912
Size: 6322
KasI G'GCGC_C
Cuts at: 2069 2069
Size: 6322
KpnI G_GTAC'C
Cuts at: 3647 3647
Size: 6322
MfeI C'AATT_G
Cuts at: 2655 3021 3165 3921 2655
Size: 366 144 756 5056
Fragments arranged by size:
5056 756 366 144
MluI A'CGCG_T
Cuts at: 6198 6198
Size: 6322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2417 3531 5436 5620 1305
Size: 1112 1114 1905 184 2007
Fragments arranged by size:
2007 1905 1114 1112 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4500
Fragments arranged by size:
4500 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4280 4639 4798 518
Size: 648 3114 359 159 2042
Fragments arranged by size:
3114 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2061 3738 4415 5563 5808 1606
Size: 455 1677 677 1148 245 2120
Fragments arranged by size:
2120 1677 1148 677 455 245
NaeI GCC'GGC
Cuts at: 2053 3412 2053
Size: 1359 4963
NarI GG'CG_CC
Cuts at: 2070 2070
Size: 6322
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5770
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6322
NgoMIV G'CCGG_C
Cuts at: 2051 3410 2051
Size: 1359 4963
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6322
NotI GC'GGCC_GC
Cuts at: 2056 3769 2056
Size: 1713 4609
NspI r_CATG'y
Cuts at: 1132 1229 2458 3070 3458 3680 5225 1132
Size: 97 1229 612 388 222 1545 2229
Fragments arranged by size:
2229 1545 1229 612 388 222 97
PasI CC'CwG_GG
Cuts at: 1348 3460 1348
Size: 2112 4210
PciI A'CATG_T
Cuts at: 2454 5221 2454
Size: 2767 3555
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6322
PmeI GTTT'AAAC
Cuts at: 3626 3626
Size: 6322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4391 4423 5904 5936 4391
Size: 32 1481 32 4777
Fragments arranged by size:
4777 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6322
PsiI TTA'TAA
Cuts at: 3892 3892
Size: 6322
PspXI vC'TCGA_Gb
Cuts at: 3618 3618
Size: 6322
PstI C_TGCA'G
Cuts at: 830 3478 3674 830
Size: 2648 196 3478
Fragments arranged by size:
3478 2648 196
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6322
SacII CC_GC'GG
Cuts at: 2062 2062
Size: 6322
SalI G'TCGA_C
Cuts at: 1936 3664 1936
Size: 1728 4594
SbfI CC_TGCA'GG
Cuts at: 3674 3674
Size: 6322
ScaI AGT'ACT
Cuts at: 4570 6282 4570
Size: 1712 4610
SfcI C'TryA_G
Cuts at: 826 1045 3429 3474 3670 4805 5486 5677 826
Size: 219 2384 45 196 1135 681 191 1471
Fragments arranged by size:
2384 1471 1135 681 219 196 191 45
SfoI GGC'GCC
Cuts at: 2071 2071
Size: 6322
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6322
SmaI CCC'GGG
Cuts at: 1872 3649 1872
Size: 1777 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2295 3125 3618 4426 5327 5589
Size: 197 926 352 830 493 808 901 262
Cuts at: 5589 820
Size: 1553
Fragments arranged by size:
1553 926 901 830 808 493 352 262
197
SnaBI TAC'GTA
Cuts at: 493 4113 493
Size: 3620 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6322
SphI G_CATG'C
Cuts at: 1132 3458 3680 1132
Size: 2326 222 3774
Fragments arranged by size:
3774 2326 222
SspI AAT'ATT
Cuts at: 5 52 3320 4246 5
Size: 47 3268 926 2081
Fragments arranged by size:
3268 2081 926 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6322
StyI C'CwwG_G
Cuts at: 513 1065 2158 3761 513
Size: 552 1093 1603 3074
Fragments arranged by size:
3074 1603 1093 552
SwaI ATTT'AAAT
Cuts at: 3105 3105
Size: 6322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2708 2838 4349 4366 4519 4704 5149
Size: 1255 1128 130 1511 17 153 185 445
Cuts at: 5149 325
Size: 1498
Fragments arranged by size:
1511 1498 1255 1128 445 185 153 130
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4568 6280 96
Size: 274 80 33 51 1443 2591 1712 138
Fragments arranged by size:
2591 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6282 6314 6282
Size: 32 6290
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6322
XbaI T'CTAG_A
Cuts at: 3658 3658
Size: 6322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2275 2779 2275
Size: 504 5818
XhoI C'TCGA_G
Cuts at: 1943 3618 1943
Size: 1675 4647
XmaI C'CCGG_G
Cuts at: 1870 3647 1870
Size: 1777 4545
XmnI GAAnn'nnTTC
Cuts at: 3579 4451 3579
Size: 872 5450
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4130 276
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI