Restriction map for
pFN21AB5378
(Circular) (Six-base) MAPSORT of: pFN21AB5378.seq Check: 1270 from: 1 to: 6922
pFN21AB5378 6922 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3034 3034
Size: 6922
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4732 278
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4265 1937
Size: 2328 4594
Acc65I G'GTAC_C
Cuts at: 4243 4243
Size: 6922
AclI AA'CG_TT
Cuts at: 1563 5049 5422 1563
Size: 3486 373 3063
Fragments arranged by size:
3486 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2063 2487 2673 2847 3273 3831 4985 6369
Size: 431 424 186 174 426 558 1154 1384
Cuts at: 6369 6637 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1154 558 431 426 424 268
186 174
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6725
AflIII A'CryG_T
Cuts at: 1203 2139 2217 5821 6798 1203
Size: 936 78 3604 977 1327
Fragments arranged by size:
3604 1327 977 936 78
AgeI A'CCGG_T
Cuts at: 5753 5753
Size: 6922
AhdI GACnn_n'nnGTC
Cuts at: 5651 5651
Size: 6922
AleI CACnn'nnGTG
Cuts at: 1166 3077 3203 3692 1166
Size: 1911 126 489 4396
Fragments arranged by size:
4396 1911 489 126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6922
AlwNI CAG_nnn'CTG
Cuts at: 3271 4665 6237 3271
Size: 1394 1572 3956
Fragments arranged by size:
3956 1572 1394
ApaLI G'TGCA_C
Cuts at: 2221 4979 6135 2221
Size: 2758 1156 3008
Fragments arranged by size:
3008 2758 1156
ApoI r'AATT_y
Cuts at: 1493 2559 3351 3956 4231 4457 4609 6718 1493
Size: 1066 792 605 275 226 152 2109 1697
Fragments arranged by size:
2109 1697 1066 792 605 275 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5476 160
Size: 872 4444 1606
Fragments arranged by size:
4444 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
AvaI C'yCGr_G
Cuts at: 1870 1943 4247 1870
Size: 73 2304 4545
Fragments arranged by size:
4545 2304 73
BamHI G'GATC_C
Cuts at: 2518 3643 4252 4619 2518
Size: 1125 609 367 4821
Fragments arranged by size:
4821 1125 609 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2477 3694 4243 5698 618
Size: 325 217 627 690 1217 549 1455 1842
Fragments arranged by size:
1842 1455 1217 690 627 549 325 217
BanII G_rGCy'C
Cuts at: 1391 2880 2979 3856 4241 1391
Size: 1489 99 877 385 4072
Fragments arranged by size:
4072 1489 877 385 99
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3170 928
Size: 971 1271 4680
Fragments arranged by size:
4680 1271 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3051 3085 5113 5147 1903
Size: 34 50 34 1030 34 2028 34 3678
Fragments arranged by size:
3678 2028 1030 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4814 6024 4814
Size: 1210 5712
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6922
BfrBI ATG'CAT
Cuts at: 2446 2446
Size: 6922
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3719 5533 136
Size: 107 122 71 631 605 2047 1814 1525
Fragments arranged by size:
2047 1814 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2245 2910 4125 6917 2245
Size: 665 1215 2792 2250
Fragments arranged by size:
2792 2250 1215 665
BlpI GC'TnA_GC
Cuts at: 3911 4339 3911
Size: 428 6494
Bme1580I G_kGCm'C
Cuts at: 1790 2225 2482 4983 6139 1790
Size: 435 257 2501 1156 2573
Fragments arranged by size:
2573 2501 1156 435 257
BmgBI CAC'GTC
Cuts at: 1318 2029 1318
Size: 711 6211
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3727 4653 5611
Size: 416 53 83 399 24 2310 926 958
Cuts at: 5611 442
Size: 1753
Fragments arranged by size:
2310 1753 958 926 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2852 1056
Size: 1796 5126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3870 3959 5582 1239
Size: 2631 89 1623 2579
Fragments arranged by size:
2631 2579 1623 89
Bpu10I CC'TnA_GC
Cuts at: 4059 4059
Size: 6922
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2112 2563 2720 5047 5912 6210 2112
Size: 451 157 2327 865 298 2824
Fragments arranged by size:
2824 2327 865 451 298 157
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2553 3216 3554 4035 5585 882
Size: 617 1054 663 338 481 1550 2219
Fragments arranged by size:
2219 1550 1054 663 617 481 338
BsaAI yAC'GTr
Cuts at: 493 1204 2809 4713 493
Size: 711 1605 1904 2702
Fragments arranged by size:
2702 1904 1605 711
BsaBI GATnn'nnATC
Cuts at: 6916 6916
Size: 6922
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4729 5111 5746 275
Size: 53 83 186 936 3196 382 635 1451
Fragments arranged by size:
3196 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2091 5355 5753 6027 6174 6585 2091
Size: 3264 398 274 147 411 2428
Fragments arranged by size:
3264 2428 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2033 2215 2245 2317
Size: 30 165 30 600 30 182 30 72
Cuts at: 2317 2347 2568 2598 2996 3026 3822 3852 1178
Size: 30 221 30 398 30 796 30 4248
Fragments arranged by size:
4248 796 600 398 221 182 165 72
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3068 3557 1186
Size: 1882 489 4551
Fragments arranged by size:
4551 1882 489
BseYI C'CCAG_C
Cuts at: 3172 3669 4025 6125 6621 3172
Size: 497 356 2100 496 3473
Fragments arranged by size:
3473 2100 497 496 356
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2375 3055 3059 3230 1163
Size: 573 639 680 4 171 4855
Fragments arranged by size:
4855 680 639 573 171 4
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4372 5133 6161 1540
Size: 275 186 2371 761 1028 2301
Fragments arranged by size:
2371 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2225 2880 3192 4060 4983 5068 6139 2225
Size: 655 312 868 923 85 1071 3008
Fragments arranged by size:
3008 1071 923 868 655 312 85
BsmI GAATG_Cn'
Cuts at: 4433 4526 4433
Size: 93 6829
BsmBI CGTCTCn'nnnn_
Cuts at: 4151 4151
Size: 6922
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2225 2482 2880 2979 3192 3856 4060
Size: 399 435 257 398 99 213 664 204
Cuts at: 4060 4241 4983 5068 6139 1391
Size: 181 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 664 435 399 398 257
213 204 181 99 85
BspEI T'CCGG_A
Cuts at: 6585 6585
Size: 6922
BspHI T'CATG_A
Cuts at: 4811 6541 4811
Size: 1730 5192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3034 3475 4277 844
Size: 2190 441 802 3489
Fragments arranged by size:
3489 2190 802 441
BsrBI CCG'CTC
Cuts at: 1394 1823 4809 1394
Size: 429 2986 3507
Fragments arranged by size:
3507 2986 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2598 3123 4020 5417 5591 65
Size: 1183 1350 525 897 1397 174 1396
Fragments arranged by size:
1397 1396 1350 1183 897 525 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5566 5753 1700
Size: 110 3756 187 2869
Fragments arranged by size:
3756 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2214 96
Size: 2118 4804
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6922
BssSI C'ACGA_G
Cuts at: 4982 5994 4982
Size: 1012 5910
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2283 3046 3101 3940 794
Size: 1489 763 55 839 3776
Fragments arranged by size:
3776 1489 839 763 55
BstBI TT'CG_AA
Cuts at: 5726 5726
Size: 6922
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6922
BstXI CCAn_nnnn'nTGG
Cuts at: 2302 3205 2302
Size: 903 6019
BstYI r'GATC_y
Cuts at: 1972 2245 2518 2910 3643 4125 4252 4619 5004
Size: 273 273 392 733 482 127 367 385
Cuts at: 5004 5021 6473 6559 6571 6917 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 733 482 392 385 367 346
273 273 127 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2339 2339
Size: 6922
BtgI C'CryG_G
Cuts at: 513 1065 3340 4037 513
Size: 552 2275 697 3398
Fragments arranged by size:
3398 2275 697 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3994 487
Size: 683 56 2768 3415
Fragments arranged by size:
3415 2768 683 56
BtsI GCAGTG_nn'
Cuts at: 2695 3435 4438 5232 5252 2695
Size: 740 1003 794 20 4365
Fragments arranged by size:
4365 1003 794 740 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6922
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6887
DraI TTT'AAA
Cuts at: 4226 4226
Size: 6922
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5929 809
Size: 507 4613 1802
Fragments arranged by size:
4613 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 4034 4369
Size: 54 1425 50 275 18 429 1775 335
Cuts at: 4369 5258 6887 8
Size: 889 1629 43
Fragments arranged by size:
1775 1629 1425 889 429 335 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4369 1537
Size: 275 2557 4090
Fragments arranged by size:
4090 2557 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3279 3557 3807 3990 4852 1353
Size: 1926 278 250 183 862 3423
Fragments arranged by size:
3423 1926 862 278 250 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5509 5883 6029 387
Size: 1064 138 3920 374 146 1280
Fragments arranged by size:
3920 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2878 2878
Size: 6922
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2487 2673 2847 3273 3831 3870
Size: 393 431 424 186 174 426 558 39
Cuts at: 3870 3959 4985 5582 6369 6637 1239
Size: 89 1026 597 787 268 1524
Fragments arranged by size:
1524 1026 787 597 558 431 426 424
393 268 186 174 89 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2410 2410
Size: 6922
EcoO109I rG'GnC_Cy
Cuts at: 2840 3268 3797 2840
Size: 428 529 5965
Fragments arranged by size:
5965 529 428
EcoRI G'AATT_C
Cuts at: 3351 3956 4231 4609 3351
Size: 605 275 378 5664
Fragments arranged by size:
5664 605 378 275
FspI TGC'GCA
Cuts at: 5428 5428
Size: 6922
HaeII r_GCGC'y
Cuts at: 1678 6069 1678
Size: 4391 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2409 2434 2441 2466 2568
Size: 32 135 32 837 25 7 25 102
Cuts at: 2568 2600 5563 5595 5637 5669 1373
Size: 32 2963 32 42 32 2626
Fragments arranged by size:
2963 2626 837 135 102 42 32 32
32 32 32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 4266 4512 669
Size: 988 281 2328 246 3079
Fragments arranged by size:
3079 2328 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6922
HpaI GTT'AAC
Cuts at: 4512 4512
Size: 6922
KpnI G_GTAC'C
Cuts at: 4247 4247
Size: 6922
MfeI C'AATT_G
Cuts at: 2523 4203 4521 2523
Size: 1680 318 4924
Fragments arranged by size:
4924 1680 318
MluI A'CGCG_T
Cuts at: 6798 6798
Size: 6922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2703 3381 6036 6220 1305
Size: 1398 678 2655 184 2007
Fragments arranged by size:
2655 2007 1398 678 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 10
Size: 54 1425 343 429 4671
Fragments arranged by size:
4671 1425 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3077 3203 3692 4013 4880 5239 5398
Size: 648 1911 126 489 321 867 359 159
Cuts at: 5398 518
Size: 2042
Fragments arranged by size:
2042 1911 867 648 489 359 321 159
126
MspA1I CmG'CkG
Cuts at: 1606 3416 4338 5015 6163 6408 1606
Size: 1810 922 677 1148 245 2120
Fragments arranged by size:
2120 1810 1148 922 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3340 4037 513
Size: 552 2275 697 3398
Fragments arranged by size:
3398 2275 697 552
NdeI CA'TA_TG
Cuts at: 387 2227 387
Size: 1840 5082
NheI G'CTAG_C
Cuts at: 1052 2848 1052
Size: 1796 5126
NotI GC'GGCC_GC
Cuts at: 4369 4369
Size: 6922
NsiI A_TGCA'T
Cuts at: 2448 2448
Size: 6922
NspI r_CATG'y
Cuts at: 1132 1229 2143 2221 2332 3487 4280 5825 1132
Size: 97 914 78 111 1155 793 1545 2229
Fragments arranged by size:
2229 1545 1155 914 793 111 97 78
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6922
PciI A'CATG_T
Cuts at: 2139 2217 5821 2139
Size: 78 3604 3240
Fragments arranged by size:
3604 3240 78
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3704 1108
Size: 2596 4326
PmeI GTTT'AAAC
Cuts at: 4226 4226
Size: 6922
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4991 5023 6504 6536 4991
Size: 32 1481 32 5377
Fragments arranged by size:
5377 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2840 3797 2840
Size: 957 5965
PshAI GACnn'nnGTC
Cuts at: 1613 3546 1613
Size: 1933 4989
PsiI TTA'TAA
Cuts at: 4492 4492
Size: 6922
PstI C_TGCA'G
Cuts at: 830 4274 830
Size: 3444 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6922
PvuII CAG'CTG
Cuts at: 3416 3416
Size: 6922
SacI G_AGCT'C
Cuts at: 2880 2880
Size: 6922
SalI G'TCGA_C
Cuts at: 1936 4264 1936
Size: 2328 4594
SapI GCTCTTCn'nnn_
Cuts at: 3990 3990
Size: 6922
SbfI CC_TGCA'GG
Cuts at: 4274 4274
Size: 6922
ScaI AGT'ACT
Cuts at: 5170 6882 5170
Size: 1712 5210
SexAI A'CCwGG_T
Cuts at: 2926 3225 2926
Size: 299 6623
SfcI C'TryA_G
Cuts at: 826 1045 2181 2993 4270 5405 6086 6277 826
Size: 219 1136 812 1277 1135 681 191 1471
Fragments arranged by size:
1471 1277 1136 1135 812 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6922
SmaI CCC'GGG
Cuts at: 1872 4249 1872
Size: 2377 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2127 2542 2735 5026 5927 6189
Size: 197 926 184 415 193 2291 901 262
Cuts at: 6189 820
Size: 1553
Fragments arranged by size:
2291 1553 926 901 415 262 197 193
184
SnaBI TAC'GTA
Cuts at: 493 4713 493
Size: 4220 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6922
SphI G_CATG'C
Cuts at: 1132 4280 1132
Size: 3148 3774
SspI AAT'ATT
Cuts at: 5 52 4846 5
Size: 47 4794 2081
Fragments arranged by size:
4794 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6922
StyI C'CwwG_G
Cuts at: 513 1065 2014 2428 3340 3944 4037 4361 513
Size: 552 949 414 912 604 93 324 3074
Fragments arranged by size:
3074 949 912 604 552 414 324 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3558 4949 4966 5119 5304 5749 325
Size: 1255 1978 1391 17 153 185 445 1498
Fragments arranged by size:
1978 1498 1391 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2214 3248 5168
Size: 274 80 33 51 1443 237 1034 1920
Cuts at: 5168 6880 96
Size: 1712 138
Fragments arranged by size:
1920 1712 1443 1034 274 237 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2215 2247 2335 2367 2996 3028 6882 6914 2215
Size: 32 88 32 629 32 3854 32 2223
Fragments arranged by size:
3854 2223 629 88 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6922
XbaI T'CTAG_A
Cuts at: 2551 4258 2551
Size: 1707 5215
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6922
XmaI C'CCGG_G
Cuts at: 1870 4247 1870
Size: 2377 4545
XmnI GAAnn'nnTTC
Cuts at: 3351 3408 5051 3351
Size: 57 1643 5222
Fragments arranged by size:
5222 1643 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4730 276
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI XcmI