Restriction map for
pFN21AB5366
(Circular) (Six-base) MAPSORT of: pFN21AB5366.seq Check: 9789 from: 1 to: 5338
pFN21AB5366 5338 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3148 278
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2681 1937
Size: 744 4594
Acc65I G'GTAC_C
Cuts at: 2199 2659 2199
Size: 460 4878
AclI AA'CG_TT
Cuts at: 1563 3465 3838 1563
Size: 1902 373 3063
Fragments arranged by size:
3063 1902 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2215 2375 2448 3401 4785 5053 1632
Size: 583 160 73 953 1384 268 1917
Fragments arranged by size:
1917 1384 953 583 268 160 73
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5141
AflIII A'CryG_T
Cuts at: 1203 4237 5214 1203
Size: 3034 977 1327
Fragments arranged by size:
3034 1327 977
AgeI A'CCGG_T
Cuts at: 2461 4169 2461
Size: 1708 3630
AhdI GACnn_n'nnGTC
Cuts at: 4067 4067
Size: 5338
AleI CACnn'nnGTG
Cuts at: 1166 2210 1166
Size: 1044 4294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5338
AlwNI CAG_nnn'CTG
Cuts at: 2353 3081 4653 2353
Size: 728 1572 3038
Fragments arranged by size:
3038 1572 728
ApaLI G'TGCA_C
Cuts at: 3395 4551 3395
Size: 1156 4182
ApoI r'AATT_y
Cuts at: 1493 2527 2647 2873 3025 5134 1493
Size: 1034 120 226 152 2109 1697
Fragments arranged by size:
2109 1697 1034 226 152 120
AscI GG'CGCG_CC
Cuts at: 2132 2132
Size: 5338
AseI AT'TA_AT
Cuts at: 160 1032 3892 160
Size: 872 2860 1606
Fragments arranged by size:
2860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
AvaI C'yCGr_G
Cuts at: 1870 1943 2502 2663 1870
Size: 73 559 161 4545
Fragments arranged by size:
4545 559 161 73
BamHI G'GATC_C
Cuts at: 2299 2668 3035 2299
Size: 369 367 4602
Fragments arranged by size:
4602 369 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2199 2659 4114 618
Size: 325 217 627 221 191 460 1455 1842
Fragments arranged by size:
1842 1455 627 460 325 221 217 191
BanII G_rGCy'C
Cuts at: 1391 2247 2657 1391
Size: 856 410 4072
Fragments arranged by size:
4072 856 410
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 5338
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3529 3563 1903
Size: 34 1592 34 3678
Fragments arranged by size:
3678 1592 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4440 3230
Size: 1210 4128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2022 3949 136
Size: 107 122 71 631 605 350 1927 1525
Fragments arranged by size:
1927 1525 631 605 350 122 107 71
BglII A'GATC_T
Cuts at: 5333 5333
Size: 5338
BlpI GC'TnA_GC
Cuts at: 2755 2755
Size: 5338
Bme1580I G_kGCm'C
Cuts at: 1790 3399 4555 1790
Size: 1609 1156 2573
Fragments arranged by size:
2573 1609 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3069 4027 442
Size: 416 53 83 399 24 1652 958 1753
Fragments arranged by size:
1753 1652 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5338
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 3998 1239
Size: 1019 1740 2579
Fragments arranged by size:
2579 1740 1019
Bpu10I CC'TnA_GC
Cuts at: 2246 2246
Size: 5338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3463 4328 4626 3463
Size: 865 298 4175
Fragments arranged by size:
4175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4001 882
Size: 617 2502 2219
Fragments arranged by size:
2502 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2076 3129 493
Size: 711 872 1053 2702
Fragments arranged by size:
2702 1053 872 711
BsaBI GATnn'nnATC
Cuts at: 5332 5332
Size: 5338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3145 3527 4162
Size: 53 83 186 936 476 1136 382 635
Cuts at: 4162 275
Size: 1451
Fragments arranged by size:
1451 1136 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2456 2461 2475 3771 4169 4443 4590 5001 2456
Size: 5 14 1296 398 274 147 411 2793
Fragments arranged by size:
2793 1296 411 398 274 147 14 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5113
Fragments arranged by size:
5113 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2320 1186
Size: 1069 65 4204
Fragments arranged by size:
4204 1069 65
BseYI C'CCAG_C
Cuts at: 2012 2448 4541 5037 2012
Size: 436 2093 496 2313
Fragments arranged by size:
2313 2093 496 436
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2366 1163
Size: 573 630 4135
Fragments arranged by size:
4135 630 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2224 2788 3549 4577 1540
Size: 275 186 223 564 761 1028 2301
Fragments arranged by size:
2301 1028 761 564 275 223 186
BsiHKAI G_wGCw'C
Cuts at: 2247 3399 3484 4555 2247
Size: 1152 85 1071 3030
Fragments arranged by size:
3030 1152 1071 85
BsmI GAATG_Cn'
Cuts at: 2259 2849 2942 2259
Size: 590 93 4655
Fragments arranged by size:
4655 590 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 2657 3399 3484 4555 1391
Size: 399 457 410 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 457 410 399 85
BspEI T'CCGG_A
Cuts at: 2475 5001 2475
Size: 2526 2812
BspHI T'CATG_A
Cuts at: 3227 4957 3227
Size: 1730 3608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2693 844
Size: 1849 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2367 3225 1394
Size: 429 544 858 3507
Fragments arranged by size:
3507 858 544 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3833 4007 65
Size: 1183 2585 174 1396
Fragments arranged by size:
2585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2089 2219 2255 2461 3982 4169 1700
Size: 110 279 130 36 206 1521 187 2869
Fragments arranged by size:
2869 1521 279 206 187 130 110 36
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5338
BssHII G'CGCG_C
Cuts at: 1925 2132 2215 1925
Size: 207 83 5048
Fragments arranged by size:
5048 207 83
BssSI C'ACGA_G
Cuts at: 3398 4410 3398
Size: 1012 4326
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5338
BstBI TT'CG_AA
Cuts at: 4142 4142
Size: 5338
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5338
BstYI r'GATC_y
Cuts at: 1972 2100 2299 2668 3035 3420 3437 4889 4975
Size: 128 199 369 367 385 17 1452 86
Cuts at: 4975 4987 5333 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 369 367 346 199 128
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2190 2224 2322 513
Size: 552 939 186 34 98 3529
Fragments arranged by size:
3529 939 552 186 98 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2125 487
Size: 683 56 899 3700
Fragments arranged by size:
3700 899 683 56
BtsI GCAGTG_nn'
Cuts at: 2854 3648 3668 2854
Size: 794 20 4524
Fragments arranged by size:
4524 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5303
DraI TTT'AAA
Cuts at: 2642 2642
Size: 5338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4345 809
Size: 507 3029 1802
Fragments arranged by size:
3029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2221 2253
Size: 54 1425 50 275 18 240 151 32
Cuts at: 2253 2442 2785 3674 5303 8
Size: 189 343 889 1629 43
Fragments arranged by size:
1629 1425 889 343 275 240 189 151
54 50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 2221 2785 1537
Size: 275 409 564 4090
Fragments arranged by size:
4090 564 409 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2387 2514 2582 3268 1353
Size: 1034 127 68 686 3423
Fragments arranged by size:
3423 1034 686 127 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2326 3925 4299 4445 387
Size: 1064 138 737 1599 374 146 1280
Fragments arranged by size:
1599 1280 1064 737 374 146 138
EcoICRI GAG'CTC
Cuts at: 2245 2245
Size: 5338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2215 2258 2375 2448 3401 3998 4785
Size: 393 583 43 117 73 953 597 787
Cuts at: 4785 5053 1239
Size: 268 1524
Fragments arranged by size:
1524 953 787 597 583 393 268 117
73 43
EcoO109I rG'GnC_Cy
Cuts at: 2162 2162
Size: 5338
EcoRI G'AATT_C
Cuts at: 2647 3025 2647
Size: 378 4960
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 5338
FspI TGC'GCA
Cuts at: 3844 3844
Size: 5338
HaeII r_GCGC'y
Cuts at: 1678 2012 4485 1678
Size: 334 2473 2531
Fragments arranged by size:
2531 2473 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3979 4011 4053 4085 1373
Size: 32 135 32 2407 32 42 32 2626
Fragments arranged by size:
2626 2407 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2682 2928 669
Size: 988 281 744 246 3079
Fragments arranged by size:
3079 988 744 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5338
HpaI GTT'AAC
Cuts at: 2928 2928
Size: 5338
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 5338
KpnI G_GTAC'C
Cuts at: 2203 2663 2203
Size: 460 4878
MfeI C'AATT_G
Cuts at: 2937 2937
Size: 5338
MluI A'CGCG_T
Cuts at: 5214 5214
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4452 4636 1305
Size: 3147 184 2007
Fragments arranged by size:
3147 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2072 2444 10
Size: 54 1425 343 240 372 2904
Fragments arranged by size:
2904 1425 372 343 240 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 2294 3296 3655 3814 518
Size: 648 1044 84 1002 359 159 2042
Fragments arranged by size:
2042 1044 1002 648 359 159 84
MspA1I CmG'CkG
Cuts at: 1606 2062 2226 2350 2452 2754 3431 4579 4824
Size: 456 164 124 102 302 677 1148 245
Cuts at: 4824 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 456 302 245 164 124
102
NaeI GCC'GGC
Cuts at: 2091 2221 2257 2091
Size: 130 36 5172
Fragments arranged by size:
5172 130 36
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 5338
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3847
Fragments arranged by size:
3847 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5338
NgoMIV G'CCGG_C
Cuts at: 2089 2219 2255 2089
Size: 130 36 5172
Fragments arranged by size:
5172 130 36
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5338
NotI GC'GGCC_GC
Cuts at: 2785 2785
Size: 5338
NspI r_CATG'y
Cuts at: 1132 1229 2696 4241 1132
Size: 97 1467 1545 2229
Fragments arranged by size:
2229 1545 1467 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5338
PciI A'CATG_T
Cuts at: 4237 4237
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5338
PmeI GTTT'AAAC
Cuts at: 2642 2642
Size: 5338
PmlI CAC'GTG
Cuts at: 2076 2076
Size: 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 4920 4952 3407
Size: 32 1481 32 3793
Fragments arranged by size:
3793 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2162 2162
Size: 5338
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5338
PsiI TTA'TAA
Cuts at: 2908 2908
Size: 5338
PspXI vC'TCGA_Gb
Cuts at: 2502 2502
Size: 5338
PstI C_TGCA'G
Cuts at: 830 2337 2690 830
Size: 1507 353 3478
Fragments arranged by size:
3478 1507 353
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5338
PvuII CAG'CTG
Cuts at: 2452 2452
Size: 5338
RsrII CG'GwC_CG
Cuts at: 2459 2459
Size: 5338
SacI G_AGCT'C
Cuts at: 2247 2247
Size: 5338
SacII CC_GC'GG
Cuts at: 2227 2227
Size: 5338
SalI G'TCGA_C
Cuts at: 1936 2680 1936
Size: 744 4594
SanDI GG'GwC_CC
Cuts at: 2162 2162
Size: 5338
SapI GCTCTTCn'nnn_
Cuts at: 2582 2582
Size: 5338
SbfI CC_TGCA'GG
Cuts at: 2337 2690 2337
Size: 353 4985
ScaI AGT'ACT
Cuts at: 3586 5298 3586
Size: 1712 3626
SfcI C'TryA_G
Cuts at: 826 1045 2333 2686 3821 4502 4693 826
Size: 219 1288 353 1135 681 191 1471
Fragments arranged by size:
1471 1288 1135 681 353 219 191
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 5338
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5338
SmaI CCC'GGG
Cuts at: 1872 2665 1872
Size: 793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2502 3442 4343 4605 820
Size: 197 926 559 940 901 262 1553
Fragments arranged by size:
1553 940 926 901 559 262 197
SnaBI TAC'GTA
Cuts at: 493 3129 493
Size: 2636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5338
SphI G_CATG'C
Cuts at: 1132 2696 1132
Size: 1564 3774
SspI AAT'ATT
Cuts at: 5 52 3262 5
Size: 47 3210 2081
Fragments arranged by size:
3210 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5338
StyI C'CwwG_G
Cuts at: 513 1065 2004 2127 2601 2777 513
Size: 552 939 123 474 176 3074
Fragments arranged by size:
3074 939 552 474 176 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2462 3365 3382 3535 3720 4165 325
Size: 1255 882 903 17 153 185 445 1498
Fragments arranged by size:
1498 1255 903 882 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3584 5296 96
Size: 274 80 33 51 1443 1607 1712 138
Fragments arranged by size:
1712 1607 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5298 5330 5298
Size: 32 5306
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5338
XbaI T'CTAG_A
Cuts at: 2674 2674
Size: 5338
XhoI C'TCGA_G
Cuts at: 1943 2502 1943
Size: 559 4779
XmaI C'CCGG_G
Cuts at: 1870 2663 1870
Size: 793 4545
XmnI GAAnn'nnTTC
Cuts at: 2528 3467 2528
Size: 939 4399
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3146 276
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI NruI NsiI PacI PfoI PspOMI PsrI
SexAI SfiI SrfI SwaI XcmI