Restriction map for
pFN21AB5364
(Circular) (Six-base) MAPSORT of: pFN21AB5364.seq Check: 6692 from: 1 to: 5890
pFN21AB5364 5890 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2278 2278
Size: 5890
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3700 278
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3111 3233 1937
Size: 1174 122 4594
Fragments arranged by size:
4594 1174 122
Acc65I G'GTAC_C
Cuts at: 3211 3211
Size: 5890
AclI AA'CG_TT
Cuts at: 1563 2680 4017 4390 1563
Size: 1117 1337 373 3063
Fragments arranged by size:
3063 1337 1117 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2103 2264 3953 5337 5605 1632
Size: 471 161 1689 1384 268 1917
Fragments arranged by size:
1917 1689 1384 471 268 161
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5693
AflIII A'CryG_T
Cuts at: 1203 4789 5766 1203
Size: 3586 977 1327
Fragments arranged by size:
3586 1327 977
AgeI A'CCGG_T
Cuts at: 4721 4721
Size: 5890
AhdI GACnn_n'nnGTC
Cuts at: 4619 4619
Size: 5890
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5890
AlwNI CAG_nnn'CTG
Cuts at: 3633 5205 3633
Size: 1572 4318
ApaLI G'TGCA_C
Cuts at: 3947 5103 3947
Size: 1156 4734
ApoI r'AATT_y
Cuts at: 1493 2236 2566 2806 3199 3425 3577 5686 1493
Size: 743 330 240 393 226 152 2109 1697
Fragments arranged by size:
2109 1697 743 393 330 240 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4444 160
Size: 872 3412 1606
Fragments arranged by size:
3412 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
AvaI C'yCGr_G
Cuts at: 1870 1943 3215 1870
Size: 73 1272 4545
Fragments arranged by size:
4545 1272 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2517 2550 2517
Size: 33 5857
BamHI G'GATC_C
Cuts at: 3220 3587 3220
Size: 367 5523
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2212 3211 4666 618
Size: 325 217 627 425 999 1455 1842
Fragments arranged by size:
1842 1455 999 627 425 325 217
BanII G_rGCy'C
Cuts at: 1391 3209 1391
Size: 1818 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2457 2590 928
Size: 971 558 133 4228
Fragments arranged by size:
4228 971 558 133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4081 4115 1903
Size: 34 50 34 2060 34 3678
Fragments arranged by size:
3678 2060 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3782 4992 3782
Size: 1210 4680
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5890
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2319 4501 136
Size: 107 122 71 631 605 647 2182 1525
Fragments arranged by size:
2182 1525 647 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5885 5885
Size: 5890
BlpI GC'TnA_GC
Cuts at: 2931 3307 2931
Size: 376 5514
Bme1580I G_kGCm'C
Cuts at: 1790 3951 5107 1790
Size: 2161 1156 2573
Fragments arranged by size:
2573 2161 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5890
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3621 4579 442
Size: 416 53 83 399 24 2204 958 1753
Fragments arranged by size:
2204 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5890
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 5890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4550 1239
Size: 3311 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4015 4880 5178 4015
Size: 865 298 4727
Fragments arranged by size:
4727 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4553 882
Size: 617 3054 2219
Fragments arranged by size:
3054 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3681 493
Size: 711 2477 2702
Fragments arranged by size:
2702 2477 711
BsaBI GATnn'nnATC
Cuts at: 5884 5884
Size: 5890
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3697 4079 4714 275
Size: 53 83 186 936 2164 382 635 1451
Fragments arranged by size:
2164 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4323 4721 4995 5142 5553 4323
Size: 398 274 147 411 4660
Fragments arranged by size:
4660 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2098 2128 1178
Size: 30 165 30 695 30 4940
Fragments arranged by size:
4940 695 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5890
BseYI C'CCAG_C
Cuts at: 2836 5093 5589 2836
Size: 2257 496 3137
Fragments arranged by size:
3137 2257 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2160 1163
Size: 573 424 4893
Fragments arranged by size:
4893 573 424
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3340 4101 5129 1540
Size: 275 186 1339 761 1028 2301
Fragments arranged by size:
2301 1339 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3951 4036 5107 3951
Size: 85 1071 4734
Fragments arranged by size:
4734 1071 85
BsmI GAATG_Cn'
Cuts at: 3401 3494 3401
Size: 93 5797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3209 3951 4036 5107 1391
Size: 399 1419 742 85 1071 2174
Fragments arranged by size:
2174 1419 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5553 5553
Size: 5890
BspHI T'CATG_A
Cuts at: 3779 5509 3779
Size: 1730 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2278 3165 3245 844
Size: 1434 887 80 3489
Fragments arranged by size:
3489 1434 887 80
BsrBI CCG'CTC
Cuts at: 1394 1823 3777 1394
Size: 429 1954 3507
Fragments arranged by size:
3507 1954 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4385 4559 65
Size: 1183 3137 174 1396
Fragments arranged by size:
3137 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4534 4721 1700
Size: 110 2724 187 2869
Fragments arranged by size:
2869 2724 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5890
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5890
BssSI C'ACGA_G
Cuts at: 2889 3950 4962 2889
Size: 1061 1012 3817
Fragments arranged by size:
3817 1061 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5890
BstBI TT'CG_AA
Cuts at: 3147 4694 3147
Size: 1547 4343
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5890
BstXI CCAn_nnnn'nTGG
Cuts at: 2830 2830
Size: 5890
BstYI r'GATC_y
Cuts at: 1972 3220 3587 3972 3989 5441 5527 5539 5885
Size: 1248 367 385 17 1452 86 12 346
Cuts at: 5885 1972
Size: 1977
Fragments arranged by size:
1977 1452 1248 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3112 3112
Size: 5890
BtgI C'CryG_G
Cuts at: 513 1065 2081 2147 513
Size: 552 1016 66 4256
Fragments arranged by size:
4256 1016 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5151
Fragments arranged by size:
5151 683 56
BtsI GCAGTG_nn'
Cuts at: 3406 4200 4220 3406
Size: 794 20 5076
Fragments arranged by size:
5076 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5855
DraI TTT'AAA
Cuts at: 3194 3194
Size: 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2661 4897 809
Size: 507 1345 2236 1802
Fragments arranged by size:
2236 1802 1345 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3337 4226 5855
Size: 54 1425 50 275 18 1507 889 1629
Cuts at: 5855 8
Size: 43
Fragments arranged by size:
1629 1507 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3337 1537
Size: 275 1525 4090
Fragments arranged by size:
4090 1525 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2337 3820 1353
Size: 984 1483 3423
Fragments arranged by size:
3423 1483 984
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4477 4851 4997 387
Size: 1064 138 2888 374 146 1280
Fragments arranged by size:
2888 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2103 2264 3953 4550 5337 5605 1239
Size: 393 471 161 1689 597 787 268 1524
Fragments arranged by size:
1689 1524 787 597 471 393 268 161
EcoNI CCTnn'n_nnAGG
Cuts at: 2118 3180 2118
Size: 1062 4828
EcoRI G'AATT_C
Cuts at: 2236 3199 3577 2236
Size: 963 378 4549
Fragments arranged by size:
4549 963 378
FspI TGC'GCA
Cuts at: 4396 4396
Size: 5890
HaeII r_GCGC'y
Cuts at: 1678 5037 1678
Size: 3359 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3062 3094 4531 4563 4605
Size: 32 135 32 1490 32 1437 32 42
Cuts at: 4605 4637 1373
Size: 32 2626
Fragments arranged by size:
2626 1490 1437 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2637 3081 3234 3480 669
Size: 988 281 699 444 153 246 3079
Fragments arranged by size:
3079 988 699 444 281 246 153
HindIII A'AGCT_T
Cuts at: 748 2611 748
Size: 1863 4027
HpaI GTT'AAC
Cuts at: 2637 3480 2637
Size: 843 5047
KpnI G_GTAC'C
Cuts at: 3215 3215
Size: 5890
MfeI C'AATT_G
Cuts at: 3489 3489
Size: 5890
MluI A'CGCG_T
Cuts at: 5766 5766
Size: 5890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2954 5004 5188 1305
Size: 1649 2050 184 2007
Fragments arranged by size:
2050 2007 1649 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4068
Fragments arranged by size:
4068 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3848 4207 4366 518
Size: 648 2682 359 159 2042
Fragments arranged by size:
2682 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2055 2366 3306 3983 5131 5376 1606
Size: 449 311 940 677 1148 245 2120
Fragments arranged by size:
2120 1148 940 677 449 311 245
NcoI C'CATG_G
Cuts at: 513 1065 2147 513
Size: 552 1082 4256
Fragments arranged by size:
4256 1082 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5890
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5890
NotI GC'GGCC_GC
Cuts at: 3337 3337
Size: 5890
NspI r_CATG'y
Cuts at: 1132 1229 2635 3248 4793 1132
Size: 97 1406 613 1545 2229
Fragments arranged by size:
2229 1545 1406 613 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5890
PciI A'CATG_T
Cuts at: 4789 4789
Size: 5890
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5890
PmeI GTTT'AAAC
Cuts at: 3194 3194
Size: 5890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2127 2159 3959 3991 5472 5504 2127
Size: 32 1800 32 1481 32 2513
Fragments arranged by size:
2513 1800 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5890
PsiI TTA'TAA
Cuts at: 3460 3460
Size: 5890
PstI C_TGCA'G
Cuts at: 830 2099 3242 830
Size: 1269 1143 3478
Fragments arranged by size:
3478 1269 1143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5890
PvuII CAG'CTG
Cuts at: 2055 2055
Size: 5890
SalI G'TCGA_C
Cuts at: 1936 3232 1936
Size: 1296 4594
SbfI CC_TGCA'GG
Cuts at: 3242 3242
Size: 5890
ScaI AGT'ACT
Cuts at: 4138 5850 4138
Size: 1712 4178
SfcI C'TryA_G
Cuts at: 826 1045 2095 3238 4373 5054 5245 826
Size: 219 1050 1143 1135 681 191 1471
Fragments arranged by size:
1471 1143 1135 1050 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5890
SmaI CCC'GGG
Cuts at: 1872 3217 1872
Size: 1345 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3994 4895 5157 820
Size: 197 926 2051 901 262 1553
Fragments arranged by size:
2051 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3681 493
Size: 3188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5890
SphI G_CATG'C
Cuts at: 1132 3248 1132
Size: 2116 3774
SspI AAT'ATT
Cuts at: 5 52 2407 3814 5
Size: 47 2355 1407 2081
Fragments arranged by size:
2355 2081 1407 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5890
StyI C'CwwG_G
Cuts at: 513 1065 2147 3329 513
Size: 552 1082 1182 3074
Fragments arranged by size:
3074 1182 1082 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2496 3917 3934 4087 4272 4717 325
Size: 1255 916 1421 17 153 185 445 1498
Fragments arranged by size:
1498 1421 1255 916 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2432 4136 5848
Size: 274 80 33 51 1443 455 1704 1712
Cuts at: 5848 96
Size: 138
Fragments arranged by size:
1712 1704 1443 455 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5850 5882 5850
Size: 32 5858
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5890
XbaI T'CTAG_A
Cuts at: 2713 3226 2713
Size: 513 5377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5890
XmaI C'CCGG_G
Cuts at: 1870 3215 1870
Size: 1345 4545
XmnI GAAnn'nnTTC
Cuts at: 2721 4019 2721
Size: 1298 4592
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3698 276
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI