Restriction map for
pFN21AB5360
(Circular) (Six-base) MAPSORT of: pFN21AB5360.seq Check: 4256 from: 1 to: 5809
pFN21AB5360 5809 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3619 278
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3152 1937
Size: 1215 4594
Acc65I G'GTAC_C
Cuts at: 3130 3130
Size: 5809
AclI AA'CG_TT
Cuts at: 1563 3936 4309 1563
Size: 2373 373 3063
Fragments arranged by size:
3063 2373 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2790 3872 5256 5524 1632
Size: 1158 1082 1384 268 1917
Fragments arranged by size:
1917 1384 1158 1082 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5612
AflIII A'CryG_T
Cuts at: 1203 4708 5685 1203
Size: 3505 977 1327
Fragments arranged by size:
3505 1327 977
AgeI A'CCGG_T
Cuts at: 4640 4640
Size: 5809
AhdI GACnn_n'nnGTC
Cuts at: 4538 4538
Size: 5809
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5809
AlwNI CAG_nnn'CTG
Cuts at: 2901 3552 5124 2901
Size: 651 1572 3586
Fragments arranged by size:
3586 1572 651
ApaLI G'TGCA_C
Cuts at: 3866 5022 3866
Size: 1156 4653
ApoI r'AATT_y
Cuts at: 1493 2612 3032 3118 3344 3496 5605 1493
Size: 1119 420 86 226 152 2109 1697
Fragments arranged by size:
2109 1697 1119 420 226 152 86
AseI AT'TA_AT
Cuts at: 160 1032 4363 160
Size: 872 3331 1606
Fragments arranged by size:
3331 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
AvaI C'yCGr_G
Cuts at: 1870 1943 3134 1870
Size: 73 1191 4545
Fragments arranged by size:
4545 1191 73
BamHI G'GATC_C
Cuts at: 3139 3506 3139
Size: 367 5442
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2861 3130 4585 618
Size: 325 217 627 1074 269 1455 1842
Fragments arranged by size:
1842 1455 1074 627 325 269 217
BanII G_rGCy'C
Cuts at: 1391 3128 1391
Size: 1737 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4838
BbvCI CC'TCA_GC
Cuts at: 2348 2348
Size: 5809
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4000 4034 1903
Size: 34 2063 34 3678
Fragments arranged by size:
3678 2063 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2467 3701 4911 2467
Size: 1234 1210 3365
Fragments arranged by size:
3365 1234 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5809
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4420 136
Size: 107 122 71 631 605 2748 1525
Fragments arranged by size:
2748 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5804 5804
Size: 5809
BlpI GC'TnA_GC
Cuts at: 3226 3226
Size: 5809
Bme1580I G_kGCm'C
Cuts at: 1790 3870 5026 1790
Size: 2080 1156 2573
Fragments arranged by size:
2573 2080 1156
BmgBI CAC'GTC
Cuts at: 1318 2536 1318
Size: 1218 4591
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2271 2451 3540
Size: 416 53 83 399 24 854 180 1089
Cuts at: 3540 4498 442
Size: 958 1753
Fragments arranged by size:
1753 1089 958 854 416 399 180 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2305 4469 1239
Size: 1066 2164 2579
Fragments arranged by size:
2579 2164 1066
Bpu10I CC'TnA_GC
Cuts at: 2348 2348
Size: 5809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3934 4799 5097 3934
Size: 865 298 4646
Fragments arranged by size:
4646 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4472 882
Size: 617 2973 2219
Fragments arranged by size:
2973 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3600 493
Size: 711 2396 2702
Fragments arranged by size:
2702 2396 711
BsaBI GATnn'nnATC
Cuts at: 2369 5803 2369
Size: 3434 2375
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3616 3998 4633 275
Size: 53 83 186 936 2083 382 635 1451
Fragments arranged by size:
2083 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4242 4640 4914 5061 5472 4242
Size: 398 274 147 411 4579
Fragments arranged by size:
4579 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2098 2128 2794 2824 1178
Size: 30 165 30 695 30 666 30 4163
Fragments arranged by size:
4163 695 666 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2802 1186
Size: 1616 4193
BseYI C'CCAG_C
Cuts at: 2208 2247 2448 5012 5508 2208
Size: 39 201 2564 496 2509
Fragments arranged by size:
2564 2509 496 201 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5236
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2483 3259 4020 5048 1540
Size: 275 186 482 776 761 1028 2301
Fragments arranged by size:
2301 1028 776 761 482 275 186
BsiHKAI G_wGCw'C
Cuts at: 3870 3955 5026 3870
Size: 85 1071 4653
Fragments arranged by size:
4653 1071 85
BsmI GAATG_Cn'
Cuts at: 3320 3413 3320
Size: 93 5716
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3128 3870 3955 5026 1391
Size: 399 1338 742 85 1071 2174
Fragments arranged by size:
2174 1338 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5472 5472
Size: 5809
BspHI T'CATG_A
Cuts at: 3698 5428 3698
Size: 1730 4079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3164 844
Size: 2320 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3696 1394
Size: 429 1873 3507
Fragments arranged by size:
3507 1873 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4304 4478 65
Size: 1183 3056 174 1396
Fragments arranged by size:
3056 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4453 4640 1700
Size: 110 2643 187 2869
Fragments arranged by size:
2869 2643 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5809
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5809
BssSI C'ACGA_G
Cuts at: 3869 4881 3869
Size: 1012 4797
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5809
BstBI TT'CG_AA
Cuts at: 4613 4613
Size: 5809
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5809
BstXI CCAn_nnnn'nTGG
Cuts at: 2554 2554
Size: 5809
BstYI r'GATC_y
Cuts at: 1972 2879 3139 3506 3891 3908 5360 5446 5458
Size: 907 260 367 385 17 1452 86 12
Cuts at: 5458 5804 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 907 385 367 346 260 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2026 2814 2026
Size: 788 5021
BtgI C'CryG_G
Cuts at: 513 1065 2547 513
Size: 552 1482 3775
Fragments arranged by size:
3775 1482 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5070
Fragments arranged by size:
5070 683 56
BtsI GCAGTG_nn'
Cuts at: 2068 3325 4119 4139 2068
Size: 1257 794 20 3738
Fragments arranged by size:
3738 1257 794 20
ClaI AT'CG_AT
Cuts at: 1554 2785 1554
Size: 1231 4578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2139 2174 529
Size: 35 1575 35 4164
Fragments arranged by size:
4164 1575 35 35
DraI TTT'AAA
Cuts at: 2463 2668 2776 3113 2463
Size: 205 108 337 5159
Fragments arranged by size:
5159 337 205 108
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4816 809
Size: 507 3500 1802
Fragments arranged by size:
3500 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3256 4145 5774
Size: 54 1425 50 275 18 1426 889 1629
Cuts at: 5774 8
Size: 43
Fragments arranged by size:
1629 1426 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3256 1537
Size: 275 1444 4090
Fragments arranged by size:
4090 1444 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3078 3739 1353
Size: 1725 661 3423
Fragments arranged by size:
3423 1725 661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4396 4770 4916 387
Size: 1064 138 2807 374 146 1280
Fragments arranged by size:
2807 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2305 2790 3872 4469 5256 5524 1239
Size: 393 673 485 1082 597 787 268 1524
Fragments arranged by size:
1524 1082 787 673 597 485 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2344 2575 2344
Size: 231 5578
EcoRI G'AATT_C
Cuts at: 2612 3118 3496 2612
Size: 506 378 4925
Fragments arranged by size:
4925 506 378
FspI TGC'GCA
Cuts at: 4315 4315
Size: 5809
HaeII r_GCGC'y
Cuts at: 1678 4956 1678
Size: 3278 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2401 2433 2465 2497 2572
Size: 32 135 32 829 32 32 32 75
Cuts at: 2572 2604 2614 2646 4450 4482 4524 4556 1373
Size: 32 10 32 1804 32 42 32 2626
Fragments arranged by size:
2626 1804 829 135 75 42 32 32
32 32 32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3153 3399 669
Size: 988 281 1215 246 3079
Fragments arranged by size:
3079 1215 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5809
HpaI GTT'AAC
Cuts at: 3399 3399
Size: 5809
KpnI G_GTAC'C
Cuts at: 3134 3134
Size: 5809
MfeI C'AATT_G
Cuts at: 3408 3408
Size: 5809
MluI A'CGCG_T
Cuts at: 5685 5685
Size: 5809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4923 5107 1305
Size: 3618 184 2007
Fragments arranged by size:
3618 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3987
Fragments arranged by size:
3987 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 2652 3767 4126 4285 518
Size: 648 1386 100 1115 359 159 2042
Fragments arranged by size:
2042 1386 1115 648 359 159 100
MspA1I CmG'CkG
Cuts at: 1606 2430 3225 3902 5050 5295 1606
Size: 824 795 677 1148 245 2120
Fragments arranged by size:
2120 1148 824 795 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2547 513
Size: 552 1482 3775
Fragments arranged by size:
3775 1482 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5809
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5809
NotI GC'GGCC_GC
Cuts at: 3256 3256
Size: 5809
NspI r_CATG'y
Cuts at: 1132 1229 2056 3167 4712 1132
Size: 97 827 1111 1545 2229
Fragments arranged by size:
2229 1545 1111 827 97
PasI CC'CwG_GG
Cuts at: 1348 2701 1348
Size: 1353 4456
PciI A'CATG_T
Cuts at: 4708 4708
Size: 5809
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2340 2406 1108
Size: 1232 66 4511
Fragments arranged by size:
4511 1232 66
PmeI GTTT'AAAC
Cuts at: 3113 3113
Size: 5809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 5391 5423 3878
Size: 32 1481 32 4264
Fragments arranged by size:
4264 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2344 2344
Size: 5809
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5809
PsiI TTA'TAA
Cuts at: 3039 3379 3039
Size: 340 5469
PstI C_TGCA'G
Cuts at: 830 2569 3161 830
Size: 1739 592 3478
Fragments arranged by size:
3478 1739 592
PvuI CG_AT'CG
Cuts at: 2001 2483 2001
Size: 482 5327
PvuII CAG'CTG
Cuts at: 2430 2430
Size: 5809
SalI G'TCGA_C
Cuts at: 1936 3151 1936
Size: 1215 4594
SapI GCTCTTCn'nnn_
Cuts at: 3078 3078
Size: 5809
SbfI CC_TGCA'GG
Cuts at: 3161 3161
Size: 5809
ScaI AGT'ACT
Cuts at: 4057 5769 4057
Size: 1712 4097
SfcI C'TryA_G
Cuts at: 826 1045 2070 2425 2565 3157 4292 4973 5164
Size: 219 1025 355 140 592 1135 681 191
Cuts at: 5164 826
Size: 1471
Fragments arranged by size:
1471 1135 1025 681 592 355 219 191
140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5809
SmaI CCC'GGG
Cuts at: 1872 3136 1872
Size: 1264 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3913 4814 5076 820
Size: 197 926 1970 901 262 1553
Fragments arranged by size:
1970 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3600 493
Size: 3107 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5809
SphI G_CATG'C
Cuts at: 1132 3167 1132
Size: 2035 3774
SspI AAT'ATT
Cuts at: 5 52 3733 5
Size: 47 3681 2081
Fragments arranged by size:
3681 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5809
StyI C'CwwG_G
Cuts at: 513 1065 2547 3248 513
Size: 552 1482 701 3074
Fragments arranged by size:
3074 1482 701 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3836 3853 4006 4191 4636 325
Size: 1255 2256 17 153 185 445 1498
Fragments arranged by size:
2256 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4055 5767 96
Size: 274 80 33 51 1443 2078 1712 138
Fragments arranged by size:
2078 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2794 2826 5769 5801 2794
Size: 32 2943 32 2802
Fragments arranged by size:
2943 2802 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5809
XbaI T'CTAG_A
Cuts at: 3145 3145
Size: 5809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2554 2855 2554
Size: 301 5508
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5809
XmaI C'CCGG_G
Cuts at: 1870 3134 1870
Size: 1264 4545
XmnI GAAnn'nnTTC
Cuts at: 3938 3938
Size: 5809
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3617 276
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI