Restriction map for
pFN21AB5348
(Circular) (Six-base) MAPSORT of: pFN21AB5348.seq Check: 6265 from: 1 to: 5875
pFN21AB5348 5875 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2281 3218 1937
Size: 344 937 4594
Fragments arranged by size:
4594 937 344
Acc65I G'GTAC_C
Cuts at: 3069 3196 3069
Size: 127 5748
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2490 3938 5322 5590 1632
Size: 858 1448 1384 268 1917
Fragments arranged by size:
1917 1448 1384 858 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 4774 5751 1203
Size: 3571 977 1327
Fragments arranged by size:
3571 1327 977
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 4604 4604
Size: 5875
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5875
AlwNI CAG_nnn'CTG
Cuts at: 3618 5190 3618
Size: 1572 4303
ApaI G_GGCC'C
Cuts at: 2904 2904
Size: 5875
ApaLI G'TGCA_C
Cuts at: 3932 5088 3932
Size: 1156 4719
ApoI r'AATT_y
Cuts at: 1493 2413 3184 3410 3562 5671 1493
Size: 920 771 226 152 2109 1697
Fragments arranged by size:
2109 1697 920 771 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 3200 1870
Size: 73 1257 4545
Fragments arranged by size:
4545 1257 73
BamHI G'GATC_C
Cuts at: 3205 3572 3205
Size: 367 5508
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3069 3196 4651 618
Size: 325 217 627 1282 127 1455 1842
Fragments arranged by size:
1842 1455 1282 627 325 217 127
BanII G_rGCy'C
Cuts at: 1391 2548 2904 3194 1391
Size: 1157 356 290 4072
Fragments arranged by size:
4072 1157 356 290
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4904
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4066 4100 1903
Size: 34 2129 34 3678
Fragments arranged by size:
3678 2129 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3767 4977 3767
Size: 1210 4665
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4486 136
Size: 107 122 71 631 605 2814 1525
Fragments arranged by size:
2814 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2495 5870 2495
Size: 3375 2500
BlpI GC'TnA_GC
Cuts at: 3292 3292
Size: 5875
Bme1580I G_kGCm'C
Cuts at: 1790 2904 3936 5092 1790
Size: 1114 1032 1156 2573
Fragments arranged by size:
2573 1156 1114 1032
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2441 3606 4564
Size: 416 53 83 399 24 1024 1165 958
Cuts at: 4564 442
Size: 1753
Fragments arranged by size:
1753 1165 1024 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3153 4535 1239
Size: 1914 1382 2579
Fragments arranged by size:
2579 1914 1382
Bpu10I CC'TnA_GC
Cuts at: 2190 2190
Size: 5875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2971 2995 4000 4865 5163 2971
Size: 24 1005 865 298 3683
Fragments arranged by size:
3683 1005 865 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4538 882
Size: 617 3039 2219
Fragments arranged by size:
3039 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2558 2759 2879 2954 3083 3666 493
Size: 711 1354 201 120 75 129 583 2702
Fragments arranged by size:
2702 1354 711 583 201 129 120 75
BsaBI GATnn'nnATC
Cuts at: 2766 5869 2766
Size: 3103 2772
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3682 4064 4699 275
Size: 53 83 186 936 2149 382 635 1451
Fragments arranged by size:
2149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4308 4706 4980 5127 5538 4308
Size: 398 274 147 411 4645
Fragments arranged by size:
4645 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5650
Fragments arranged by size:
5650 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2358 2394 2538 1186
Size: 1172 36 144 4523
Fragments arranged by size:
4523 1172 144 36
BseYI C'CCAG_C
Cuts at: 2179 5078 5574 2179
Size: 2899 496 2480
Fragments arranged by size:
2899 2480 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2484 1163
Size: 573 748 4554
Fragments arranged by size:
4554 748 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3325 4086 5114 1540
Size: 275 186 1324 761 1028 2301
Fragments arranged by size:
2301 1324 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2548 2887 3115 3936 4021 5092 2548
Size: 339 228 821 85 1071 3331
Fragments arranged by size:
3331 1071 821 339 228 85
BsmI GAATG_Cn'
Cuts at: 3386 3479 3386
Size: 93 5782
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2548 2887 2904 3115 3194 3936 4021
Size: 399 758 339 17 211 79 742 85
Cuts at: 4021 5092 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 758 742 399 339 211 85
79 17
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 3764 5494 3764
Size: 1730 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3230 844
Size: 2386 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2599 3762 1394
Size: 429 776 1163 3507
Fragments arranged by size:
3507 1163 776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4370 4544 65
Size: 1183 3122 174 1396
Fragments arranged by size:
3122 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4519 4706 1700
Size: 110 2709 187 2869
Fragments arranged by size:
2869 2709 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 2376 2688 2718 2895 3935 4947 2376
Size: 312 30 177 1040 1012 3304
Fragments arranged by size:
3304 1040 1012 312 177 30
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5875
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5875
BstXI CCAn_nnnn'nTGG
Cuts at: 2245 2245
Size: 5875
BstYI r'GATC_y
Cuts at: 1972 2495 3205 3572 3957 3974 5426 5512 5524
Size: 523 710 367 385 17 1452 86 12
Cuts at: 5524 5870 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 710 523 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3106 513
Size: 552 939 1102 3282
Fragments arranged by size:
3282 1102 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5136
Fragments arranged by size:
5136 683 56
BtsI GCAGTG_nn'
Cuts at: 2533 3391 4185 4205 2533
Size: 858 794 20 4203
Fragments arranged by size:
4203 858 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5840
DraI TTT'AAA
Cuts at: 3179 3179
Size: 5875
DraIII CAC_nnn'GTG
Cuts at: 2882 2882
Size: 5875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4882 809
Size: 507 3566 1802
Fragments arranged by size:
3566 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3322 4211 5840
Size: 54 1425 50 275 18 1492 889 1629
Cuts at: 5840 8
Size: 43
Fragments arranged by size:
1629 1492 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3322 1537
Size: 275 1510 4090
Fragments arranged by size:
4090 1510 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2039 2335 2536 2557 3805 1353
Size: 686 296 201 21 1248 3423
Fragments arranged by size:
3423 1248 686 296 201 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4462 4836 4982 387
Size: 1064 138 2873 374 146 1280
Fragments arranged by size:
2873 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2546 2546
Size: 5875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2490 3153 3938 4535 5322 5590 1239
Size: 393 858 663 785 597 787 268 1524
Fragments arranged by size:
1524 858 787 785 663 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2322 2376 2322
Size: 54 5821
EcoO109I rG'GnC_Cy
Cuts at: 2315 2366 2480 2900 2901 2315
Size: 51 114 420 1 5289
Fragments arranged by size:
5289 420 114 51 1
EcoRI G'AATT_C
Cuts at: 3184 3562 3184
Size: 378 5497
FspI TGC'GCA
Cuts at: 4381 4381
Size: 5875
HaeII r_GCGC'y
Cuts at: 1678 5022 1678
Size: 3344 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2756 2788 2969 2993 3001
Size: 32 135 32 1184 32 181 24 8
Cuts at: 3001 3025 4516 4548 4590 4622 1373
Size: 24 1491 32 42 32 2626
Fragments arranged by size:
2626 1491 1184 181 135 42 32 32
32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5875
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KpnI G_GTAC'C
Cuts at: 3073 3200 3073
Size: 127 5748
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4989 5173 1305
Size: 3684 184 2007
Fragments arranged by size:
3684 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4053
Fragments arranged by size:
4053 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 2391 2832 3833 4192 4351 518
Size: 648 1077 148 441 1001 359 159 2042
Fragments arranged by size:
2042 1077 1001 648 441 359 159 148
MspA1I CmG'CkG
Cuts at: 1606 3108 3291 3968 5116 5361 1606
Size: 1502 183 677 1148 245 2120
Fragments arranged by size:
2120 1502 1148 677 245 183
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4384
Fragments arranged by size:
4384 939 552
NdeI CA'TA_TG
Cuts at: 387 2857 387
Size: 2470 3405
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 3233 4778 1132
Size: 97 2004 1545 2229
Fragments arranged by size:
2229 2004 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2474 2915 1108
Size: 1366 441 4068
Fragments arranged by size:
4068 1366 441
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PmlI CAC'GTG
Cuts at: 2558 2759 2954 3083 2558
Size: 201 195 129 5350
Fragments arranged by size:
5350 201 195 129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2315 2366 2480 2315
Size: 51 114 5710
Fragments arranged by size:
5710 114 51
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5875
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PspOMI G'GGCC_C
Cuts at: 2900 2900
Size: 5875
PstI C_TGCA'G
Cuts at: 830 3227 830
Size: 2397 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
SacI G_AGCT'C
Cuts at: 2548 2548
Size: 5875
SacII CC_GC'GG
Cuts at: 3109 3109
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SapI GCTCTTCn'nnn_
Cuts at: 2039 2536 2039
Size: 497 5378
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 2115 4123 5835 2115
Size: 2008 1712 2155
Fragments arranged by size:
2155 2008 1712
SfcI C'TryA_G
Cuts at: 826 1045 2845 3057 3091 3223 4358 5039 5230
Size: 219 1800 212 34 132 1135 681 191
Cuts at: 5230 826
Size: 1471
Fragments arranged by size:
1800 1471 1135 681 219 212 191 132
34
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 3202 1872
Size: 1330 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2986 3010 3979 4880 5142 820
Size: 197 926 1043 24 969 901 262 1553
Fragments arranged by size:
1553 1043 969 926 901 262 197 24
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 2512 152
Size: 2360 3515
SphI G_CATG'C
Cuts at: 1132 3233 1132
Size: 2101 3774
SspI AAT'ATT
Cuts at: 5 52 3799 5
Size: 47 3747 2081
Fragments arranged by size:
3747 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5875
StyI C'CwwG_G
Cuts at: 513 1065 2004 3314 513
Size: 552 939 1310 3074
Fragments arranged by size:
3074 1310 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3902 3919 4072 4257 4702 325
Size: 1255 2322 17 153 185 445 1498
Fragments arranged by size:
2322 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2113 4121 5833
Size: 274 80 33 51 1443 136 2008 1712
Cuts at: 5833 96
Size: 138
Fragments arranged by size:
2008 1712 1443 274 138 136 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2091 2123 5835 5867 2091
Size: 32 3712 32 2099
Fragments arranged by size:
3712 2099 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5875
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5875
XmaI C'CCGG_G
Cuts at: 1870 3200 1870
Size: 1330 4545
XmnI GAAnn'nnTTC
Cuts at: 4004 4004
Size: 5875
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspXI PsrI PvuII RsrII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI