Restriction map for
pFN21AB5347
(Circular) (Six-base) MAPSORT of: pFN21AB5347.seq Check: 2142 from: 1 to: 6010
pFN21AB5347 6010 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2260 2260
Size: 6010
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2529 3820 278
Size: 53 83 186 936 993 1291 2468
Fragments arranged by size:
2468 1291 993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2022 3353 1937
Size: 85 1331 4594
Fragments arranged by size:
4594 1331 85
Acc65I G'GTAC_C
Cuts at: 3246 3331 3246
Size: 85 5925
AclI AA'CG_TT
Cuts at: 1563 4137 4510 1563
Size: 2574 373 3063
Fragments arranged by size:
3063 2574 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2057 3245 4073 5457 5725 1632
Size: 425 1188 828 1384 268 1917
Fragments arranged by size:
1917 1384 1188 828 425 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5813
AflIII A'CryG_T
Cuts at: 1203 2082 2226 4909 5886 1203
Size: 879 144 2683 977 1327
Fragments arranged by size:
2683 1327 977 879 144
AgeI A'CCGG_T
Cuts at: 4841 4841
Size: 6010
AhdI GACnn_n'nnGTC
Cuts at: 4739 4739
Size: 6010
AleI CACnn'nnGTG
Cuts at: 1166 2760 1166
Size: 1594 4416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2411 2624 3230 1184
Size: 1227 213 606 3964
Fragments arranged by size:
3964 1227 606 213
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2735 2767 2735
Size: 32 5978
AlwNI CAG_nnn'CTG
Cuts at: 3256 3753 5325 3256
Size: 497 1572 3941
Fragments arranged by size:
3941 1572 497
ApaI G_GGCC'C
Cuts at: 2443 3115 2443
Size: 672 5338
ApaLI G'TGCA_C
Cuts at: 4067 5223 4067
Size: 1156 4854
ApoI r'AATT_y
Cuts at: 1493 3319 3545 3697 5806 1493
Size: 1826 226 152 2109 1697
Fragments arranged by size:
2109 1826 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4564 160
Size: 872 3532 1606
Fragments arranged by size:
3532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
AvaI C'yCGr_G
Cuts at: 1870 1943 2653 2663 3047 3085 3335 1870
Size: 73 710 10 384 38 250 4545
Fragments arranged by size:
4545 710 384 250 73 38 10
BamHI G'GATC_C
Cuts at: 3340 3707 3340
Size: 367 5643
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3246 3331 4786 618
Size: 325 217 627 1459 85 1455 1842
Fragments arranged by size:
1842 1459 1455 627 325 217 85
BanII G_rGCy'C
Cuts at: 1391 2355 2443 2630 2832 3115 3329 1391
Size: 964 88 187 202 283 214 4072
Fragments arranged by size:
4072 964 283 214 202 187 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2638 3150 928
Size: 971 739 512 3788
Fragments arranged by size:
3788 971 739 512
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2602 2636 4201 4235 1903
Size: 34 665 34 1565 34 3678
Fragments arranged by size:
3678 1565 665 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3902 5112 3902
Size: 1210 4800
BclI T'GATC_A
Cuts at: 1548 2922 1548
Size: 1374 4636
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4621 136
Size: 107 122 71 631 605 2949 1525
Fragments arranged by size:
2949 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6005 6005
Size: 6010
BlpI GC'TnA_GC
Cuts at: 3427 3427
Size: 6010
Bme1580I G_kGCm'C
Cuts at: 1790 2443 2991 3115 4071 5227 1790
Size: 653 548 124 956 1156 2573
Fragments arranged by size:
2573 1156 956 653 548 124
BmgBI CAC'GTC
Cuts at: 1318 2077 2857 1318
Size: 759 780 4471
Fragments arranged by size:
4471 780 759
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3741 4699 442
Size: 416 53 83 399 24 2324 958 1753
Fragments arranged by size:
2324 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6010
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2666 2914 2666
Size: 248 5762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2318 2759 4670 1239
Size: 1079 441 1911 2579
Fragments arranged by size:
2579 1911 1079 441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4135 5000 5298 4135
Size: 865 298 4847
Fragments arranged by size:
4847 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4673 882
Size: 617 3174 2219
Fragments arranged by size:
3174 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2026 3801 493
Size: 711 822 1775 2702
Fragments arranged by size:
2702 1775 822 711
BsaBI GATnn'nnATC
Cuts at: 2873 6004 2873
Size: 3131 2879
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2526 3817 4199 4834
Size: 53 83 186 936 993 1291 382 635
Cuts at: 4834 275
Size: 1451
Fragments arranged by size:
1451 1291 993 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3300 4443 4841 5115 5262 5673 3300
Size: 1143 398 274 147 411 3637
Fragments arranged by size:
3637 1143 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5785
Fragments arranged by size:
5785 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2144 2315 2318 1186
Size: 955 3 171 3 4878
Fragments arranged by size:
4878 955 171 3 3
BseYI C'CCAG_C
Cuts at: 2575 5213 5709 2575
Size: 2638 496 2876
Fragments arranged by size:
2876 2638 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2288 1163
Size: 573 552 4885
Fragments arranged by size:
4885 573 552
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3460 4221 5249 1540
Size: 275 186 1459 761 1028 2301
Fragments arranged by size:
2301 1459 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2052 2100 2355 2832 4071 4156 5227 2052
Size: 48 255 477 1239 85 1071 2835
Fragments arranged by size:
2835 1239 1071 477 255 85 48
BsmI GAATG_Cn'
Cuts at: 3521 3614 3521
Size: 93 5917
BsmBI CGTCTCn'nnnn_
Cuts at: 2849 2849
Size: 6010
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2100 2355 2443 2630 2832 2991
Size: 399 262 48 255 88 187 202 159
Cuts at: 2991 3115 3329 4071 4156 5227 1391
Size: 124 214 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 262 255 214 202
187 159 124 88 85 48
BspEI T'CCGG_A
Cuts at: 3300 5673 3300
Size: 2373 3637
BspHI T'CATG_A
Cuts at: 3899 5629 3899
Size: 1730 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2260 3365 844
Size: 1416 1105 3489
Fragments arranged by size:
3489 1416 1105
BsrBI CCG'CTC
Cuts at: 1394 1823 3897 1394
Size: 429 2074 3507
Fragments arranged by size:
3507 2074 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2608 4505 4679 65
Size: 1183 1360 1897 174 1396
Fragments arranged by size:
1897 1396 1360 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4654 4841 1700
Size: 110 2844 187 2869
Fragments arranged by size:
2869 2844 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6010
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6010
BssSI C'ACGA_G
Cuts at: 4070 5082 4070
Size: 1012 4998
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2104 794
Size: 1310 4700
BstBI TT'CG_AA
Cuts at: 4814 4814
Size: 6010
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6010
BstXI CCAn_nnnn'nTGG
Cuts at: 2116 2968 2116
Size: 852 5158
BstYI r'GATC_y
Cuts at: 1972 2391 2973 3183 3340 3707 4092 4109 5561
Size: 419 582 210 157 367 385 17 1452
Cuts at: 5561 5647 5659 6005 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 582 419 385 367 346 210
157 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2068 2158 2444 2713 2730 513
Size: 552 1003 90 286 269 17 3793
Fragments arranged by size:
3793 1003 552 286 269 90 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5271
Fragments arranged by size:
5271 683 56
BtsI GCAGTG_nn'
Cuts at: 3526 4320 4340 3526
Size: 794 20 5196
Fragments arranged by size:
5196 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5975
DraI TTT'AAA
Cuts at: 3314 3314
Size: 6010
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5017 809
Size: 507 3701 1802
Fragments arranged by size:
3701 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2447 3457 4346
Size: 54 1425 50 275 18 617 1010 889
Cuts at: 4346 5975 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1010 889 617 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3457 1537
Size: 275 1645 4090
Fragments arranged by size:
4090 1645 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3233 3940 1353
Size: 1880 707 3423
Fragments arranged by size:
3423 1880 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 1999 4597 4971 5117 387
Size: 1064 138 410 2598 374 146 1280
Fragments arranged by size:
2598 1280 1064 410 374 146 138
EcoICRI GAG'CTC
Cuts at: 2353 2830 2353
Size: 477 5533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2318 2759 3245 4073 4670 5457
Size: 393 425 261 441 486 828 597 787
Cuts at: 5457 5725 1239
Size: 268 1524
Fragments arranged by size:
1524 828 787 597 486 441 425 393
268 261
EcoNI CCTnn'n_nnAGG
Cuts at: 2258 2258
Size: 6010
EcoO109I rG'GnC_Cy
Cuts at: 2439 2440 2692 3043 3253 2439
Size: 1 252 351 210 5196
Fragments arranged by size:
5196 351 252 210 1
EcoRI G'AATT_C
Cuts at: 3319 3697 3319
Size: 378 5632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 6010
FspI TGC'GCA
Cuts at: 2612 4516 2612
Size: 1904 4106
HaeII r_GCGC'y
Cuts at: 1678 2019 2595 5157 1678
Size: 341 576 2562 2531
Fragments arranged by size:
2562 2531 576 341
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2819 2851 2987 3019 4651
Size: 32 135 32 1247 32 136 32 1632
Cuts at: 4651 4683 4725 4757 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1632 1247 136 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2542 2955 3354 3600 669
Size: 988 281 604 413 399 246 3079
Fragments arranged by size:
3079 988 604 413 399 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6010
HpaI GTT'AAC
Cuts at: 3600 3600
Size: 6010
KpnI G_GTAC'C
Cuts at: 3250 3335 3250
Size: 85 5925
MfeI C'AATT_G
Cuts at: 3609 3609
Size: 6010
MluI A'CGCG_T
Cuts at: 2226 5886 2226
Size: 3660 2350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5124 5308 1305
Size: 3819 184 2007
Fragments arranged by size:
3819 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4188
Fragments arranged by size:
4188 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 2636 2760 2984 3212 3968 4327
Size: 648 948 522 124 224 228 756 359
Cuts at: 4327 4486 518
Size: 159 2042
Fragments arranged by size:
2042 948 756 648 522 359 228 224
159 124
MspA1I CmG'CkG
Cuts at: 1606 2446 2715 3426 4103 5251 5496 1606
Size: 840 269 711 677 1148 245 2120
Fragments arranged by size:
2120 1148 840 711 677 269 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5458
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6010
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6010
NotI GC'GGCC_GC
Cuts at: 3457 3457
Size: 6010
NspI r_CATG'y
Cuts at: 1132 1229 2086 2662 3368 4913 1132
Size: 97 857 576 706 1545 2229
Fragments arranged by size:
2229 1545 857 706 576 97
PasI CC'CwG_GG
Cuts at: 1348 3261 1348
Size: 1913 4097
PciI A'CATG_T
Cuts at: 2082 4909 2082
Size: 2827 3183
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2428 1108
Size: 1320 4690
PfoI T'CCnGG_A
Cuts at: 2313 2313
Size: 6010
PmeI GTTT'AAAC
Cuts at: 3314 3314
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 5592 5624 4079
Size: 32 1481 32 4465
Fragments arranged by size:
4465 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2692 3043 2692
Size: 351 5659
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6010
PsiI TTA'TAA
Cuts at: 3580 3580
Size: 6010
PspOMI G'GGCC_C
Cuts at: 2439 3111 2439
Size: 672 5338
PstI C_TGCA'G
Cuts at: 830 2400 3362 830
Size: 1570 962 3478
Fragments arranged by size:
3478 1570 962
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6010
RsrII CG'GwC_CG
Cuts at: 2484 2484
Size: 6010
SacI G_AGCT'C
Cuts at: 2355 2832 2355
Size: 477 5533
SacII CC_GC'GG
Cuts at: 2447 2716 2447
Size: 269 5741
SalI G'TCGA_C
Cuts at: 1936 3352 1936
Size: 1416 4594
SanDI GG'GwC_CC
Cuts at: 3043 3043
Size: 6010
SbfI CC_TGCA'GG
Cuts at: 2400 3362 2400
Size: 962 5048
ScaI AGT'ACT
Cuts at: 2285 4258 5970 2285
Size: 1973 1712 2325
Fragments arranged by size:
2325 1973 1712
SfcI C'TryA_G
Cuts at: 826 1045 2396 3358 4493 5174 5365 826
Size: 219 1351 962 1135 681 191 1471
Fragments arranged by size:
1471 1351 1135 962 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6010
SmaI CCC'GGG
Cuts at: 1872 3087 3337 1872
Size: 1215 250 4545
Fragments arranged by size:
4545 1215 250
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4114 5015 5277 820
Size: 197 926 2171 901 262 1553
Fragments arranged by size:
2171 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3801 493
Size: 3308 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6010
SphI G_CATG'C
Cuts at: 1132 2662 3368 1132
Size: 1530 706 3774
Fragments arranged by size:
3774 1530 706
SspI AAT'ATT
Cuts at: 5 52 3934 5
Size: 47 3882 2081
Fragments arranged by size:
3882 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6010
StyI C'CwwG_G
Cuts at: 513 1065 3449 513
Size: 552 2384 3074
Fragments arranged by size:
3074 2384 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2867 3022 3253 4037 4054 4207 4392
Size: 1255 1287 155 231 784 17 153 185
Cuts at: 4392 4837 325
Size: 445 1498
Fragments arranged by size:
1498 1287 1255 784 445 231 185 155
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 2403 2916
Size: 274 80 33 51 1443 306 120 513
Cuts at: 2916 4256 5968 96
Size: 1340 1712 138
Fragments arranged by size:
1712 1443 1340 513 306 274 138 120
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2053 2085 5970 6002 2053
Size: 32 3885 32 2061
Fragments arranged by size:
3885 2061 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2690 1316
Size: 1374 4636
XbaI T'CTAG_A
Cuts at: 3346 3346
Size: 6010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2568 2568
Size: 6010
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6010
XmaI C'CCGG_G
Cuts at: 1870 3085 3335 1870
Size: 1215 250 4545
Fragments arranged by size:
4545 1215 250
XmnI GAAnn'nnTTC
Cuts at: 4139 4139
Size: 6010
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2527 3818 276
Size: 53 83 186 936 993 1291 2468
Fragments arranged by size:
2468 1291 993 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI PvuII SapI SexAI SfiI SfoI SrfI SwaI