Restriction map for
pFN21AB5337
(Circular) (Six-base) MAPSORT of: pFN21AB5337.seq Check: 5807 from: 1 to: 5479
pFN21AB5337 5479 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2403 2403
Size: 5479
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3289 278
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2822 1937
Size: 885 4594
Acc65I G'GTAC_C
Cuts at: 2800 2800
Size: 5479
AclI AA'CG_TT
Cuts at: 1563 3606 3979 1563
Size: 2043 373 3063
Fragments arranged by size:
3063 2043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2371 3542 4926 5194 1632
Size: 739 1171 1384 268 1917
Fragments arranged by size:
1917 1384 1171 739 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5282
AflIII A'CryG_T
Cuts at: 1203 4378 5355 1203
Size: 3175 977 1327
Fragments arranged by size:
3175 1327 977
AgeI A'CCGG_T
Cuts at: 4310 4310
Size: 5479
AhdI GACnn_n'nnGTC
Cuts at: 4208 4208
Size: 5479
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2435 1184
Size: 1251 4228
AlwNI CAG_nnn'CTG
Cuts at: 2394 3222 4794 2394
Size: 828 1572 3079
Fragments arranged by size:
3079 1572 828
ApaI G_GGCC'C
Cuts at: 2052 2052
Size: 5479
ApaLI G'TGCA_C
Cuts at: 3536 4692 3536
Size: 1156 4323
ApoI r'AATT_y
Cuts at: 1493 2439 2788 3014 3166 5275 1493
Size: 946 349 226 152 2109 1697
Fragments arranged by size:
2109 1697 946 349 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4033 160
Size: 872 3001 1606
Fragments arranged by size:
3001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2230 2253 2490 2804 1870
Size: 73 108 179 23 237 314 4545
Fragments arranged by size:
4545 314 237 179 108 73 23
BamHI G'GATC_C
Cuts at: 2809 3176 2809
Size: 367 5112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2415 2485 2800 4255 618
Size: 325 217 627 628 70 315 1455 1842
Fragments arranged by size:
1842 1455 628 627 325 315 217 70
BanII G_rGCy'C
Cuts at: 1391 2052 2522 2798 1391
Size: 661 470 276 4072
Fragments arranged by size:
4072 661 470 276
BbeI G_GCGC'C
Cuts at: 2489 2489
Size: 5479
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2705 928
Size: 971 806 3702
Fragments arranged by size:
3702 971 806
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3670 3704 1903
Size: 34 50 34 1649 34 3678
Fragments arranged by size:
3678 1649 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3371 4581 3371
Size: 1210 4269
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4090 136
Size: 107 122 71 631 605 2418 1525
Fragments arranged by size:
2418 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5474 5474
Size: 5479
BlpI GC'TnA_GC
Cuts at: 2896 2896
Size: 5479
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2404 2514 3540 4696 1790
Size: 262 352 110 1026 1156 2573
Fragments arranged by size:
2573 1156 1026 352 262 110
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5479
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3210 4168 442
Size: 416 53 83 399 24 1793 958 1753
Fragments arranged by size:
1793 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2111 1056
Size: 1055 4424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2570 2747 4139 1239
Size: 1331 177 1392 2579
Fragments arranged by size:
2579 1392 1331 177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3604 4469 4767 3604
Size: 865 298 4316
Fragments arranged by size:
4316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2439 4142 882
Size: 617 940 1703 2219
Fragments arranged by size:
2219 1703 940 617
BsaAI yAC'GTr
Cuts at: 493 1204 3270 493
Size: 711 2066 2702
Fragments arranged by size:
2702 2066 711
BsaBI GATnn'nnATC
Cuts at: 5473 5473
Size: 5479
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2486 3286 3668 4303
Size: 53 83 186 936 953 800 382 635
Cuts at: 4303 275
Size: 1451
Fragments arranged by size:
1451 953 936 800 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2448 2694 3912 4310 4584 4731 5142 2448
Size: 246 1218 398 274 147 411 2785
Fragments arranged by size:
2785 1218 411 398 274 246 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2443 2473 1178
Size: 30 165 30 1040 30 4184
Fragments arranged by size:
4184 1040 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2153 1186
Size: 928 39 4512
Fragments arranged by size:
4512 928 39
BseYI C'CCAG_C
Cuts at: 2653 4682 5178 2653
Size: 2029 496 2954
Fragments arranged by size:
2954 2029 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 2522 1163
Size: 573 522 264 4120
Fragments arranged by size:
4120 573 522 264
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2620 2929 3690 4718 1540
Size: 275 186 619 309 761 1028 2301
Fragments arranged by size:
2301 1028 761 619 309 275 186
BsiHKAI G_wGCw'C
Cuts at: 2456 3540 3625 4696 2456
Size: 1084 85 1071 3239
Fragments arranged by size:
3239 1084 1071 85
BsmI GAATG_Cn'
Cuts at: 2990 3083 2990
Size: 93 5386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2404 2456 2514 2522 2798 3540
Size: 399 262 352 52 58 8 276 742
Cuts at: 3540 3625 4696 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 352 276 262 85
58 52 8
BspEI T'CCGG_A
Cuts at: 5142 5142
Size: 5479
BspHI T'CATG_A
Cuts at: 3368 5098 3368
Size: 1730 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2403 2834 844
Size: 1559 431 3489
Fragments arranged by size:
3489 1559 431
BsrBI CCG'CTC
Cuts at: 1394 1823 2554 3366 1394
Size: 429 731 812 3507
Fragments arranged by size:
3507 812 731 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3974 4148 65
Size: 1183 2726 174 1396
Fragments arranged by size:
2726 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4123 4310 1700
Size: 110 2313 187 2869
Fragments arranged by size:
2869 2313 187 110
BsrGI T'GTAC_A
Cuts at: 96 2211 96
Size: 2115 3364
BssHII G'CGCG_C
Cuts at: 1925 2111 1925
Size: 186 5293
BssSI C'ACGA_G
Cuts at: 2021 2582 3539 4551 2021
Size: 561 957 1012 2949
Fragments arranged by size:
2949 1012 957 561
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5479
BstBI TT'CG_AA
Cuts at: 4283 4283
Size: 5479
BstEII G'GTnAC_C
Cuts at: 1184 2366 1184
Size: 1182 4297
BstYI r'GATC_y
Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474
Size: 837 367 385 17 1452 86 12 346
Cuts at: 5474 1972
Size: 1977
Fragments arranged by size:
1977 1452 837 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2127 513
Size: 552 1062 3865
Fragments arranged by size:
3865 1062 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4740
Fragments arranged by size:
4740 683 56
BtsI GCAGTG_nn'
Cuts at: 2080 2651 2995 3789 3809 2080
Size: 571 344 794 20 3750
Fragments arranged by size:
3750 794 571 344 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5444
DraI TTT'AAA
Cuts at: 2783 2783
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4486 809
Size: 507 3170 1802
Fragments arranged by size:
3170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2040 2559
Size: 54 1425 50 275 18 195 15 519
Cuts at: 2559 2926 3815 5444 8
Size: 367 889 1629 43
Fragments arranged by size:
1629 1425 889 519 367 275 195 54
50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2926 1537
Size: 275 1114 4090
Fragments arranged by size:
4090 1114 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3409 1353
Size: 2056 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2330 4066 4440 4586 387
Size: 1064 138 741 1736 374 146 1280
Fragments arranged by size:
1736 1280 1064 741 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2371 2570 2747 3542 4139 4926 5194
Size: 393 739 199 177 795 597 787 268
Cuts at: 5194 1239
Size: 1524
Fragments arranged by size:
1524 795 787 739 597 393 268 199
177
EcoO109I rG'GnC_Cy
Cuts at: 2587 2722 2587
Size: 135 5344
EcoRI G'AATT_C
Cuts at: 2788 3166 2788
Size: 378 5101
FspI TGC'GCA
Cuts at: 3985 3985
Size: 5479
HaeII r_GCGC'y
Cuts at: 1678 2489 2549 4626 1678
Size: 811 60 2077 2531
Fragments arranged by size:
2531 2077 811 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4120 4152 4194 4226 1373
Size: 32 135 32 2548 32 42 32 2626
Fragments arranged by size:
2626 2548 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2823 3069 669
Size: 988 281 885 246 3079
Fragments arranged by size:
3079 988 885 281 246
HindIII A'AGCT_T
Cuts at: 748 2711 748
Size: 1963 3516
HpaI GTT'AAC
Cuts at: 3069 3069
Size: 5479
KasI G'GCGC_C
Cuts at: 2485 2485
Size: 5479
KpnI G_GTAC'C
Cuts at: 2804 2804
Size: 5479
MfeI C'AATT_G
Cuts at: 3078 3078
Size: 5479
MluI A'CGCG_T
Cuts at: 5355 5355
Size: 5479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4593 4777 1305
Size: 3288 184 2007
Fragments arranged by size:
3288 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3657
Fragments arranged by size:
3657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2282 3437 3796 3955 518
Size: 648 1116 1155 359 159 2042
Fragments arranged by size:
2042 1155 1116 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2758 2895 3572 4720 4965 1606
Size: 1152 137 677 1148 245 2120
Fragments arranged by size:
2120 1152 1148 677 245 137
NarI GG'CG_CC
Cuts at: 2486 2486
Size: 5479
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4927
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5479
NheI G'CTAG_C
Cuts at: 1052 2107 1052
Size: 1055 4424
NotI GC'GGCC_GC
Cuts at: 2926 2926
Size: 5479
NspI r_CATG'y
Cuts at: 1132 1229 2837 4382 1132
Size: 97 1608 1545 2229
Fragments arranged by size:
2229 1608 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2185 2515 1348
Size: 837 330 4312
Fragments arranged by size:
4312 837 330
PciI A'CATG_T
Cuts at: 4378 4378
Size: 5479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2758 1108
Size: 1650 3829
PfoI T'CCnGG_A
Cuts at: 2331 2331
Size: 5479
PmeI GTTT'AAAC
Cuts at: 2783 2783
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 5061 5093 3548
Size: 32 1481 32 3934
Fragments arranged by size:
3934 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2587 2587
Size: 5479
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5479
PsiI TTA'TAA
Cuts at: 3049 3049
Size: 5479
PspOMI G'GGCC_C
Cuts at: 2048 2048
Size: 5479
PstI C_TGCA'G
Cuts at: 830 2346 2424 2831 830
Size: 1516 78 407 3478
Fragments arranged by size:
3478 1516 407 78
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5479
PvuII CAG'CTG
Cuts at: 2758 2758
Size: 5479
SalI G'TCGA_C
Cuts at: 1936 2821 1936
Size: 885 4594
SbfI CC_TGCA'GG
Cuts at: 2831 2831
Size: 5479
ScaI AGT'ACT
Cuts at: 3727 5439 3727
Size: 1712 3767
SfcI C'TryA_G
Cuts at: 826 1045 2342 2395 2420 2827 3962 4643 4834
Size: 219 1297 53 25 407 1135 681 191
Cuts at: 4834 826
Size: 1471
Fragments arranged by size:
1471 1297 1135 681 407 219 191 53
25
SfoI GGC'GCC
Cuts at: 2487 2487
Size: 5479
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5479
SmaI CCC'GGG
Cuts at: 1872 2053 2492 2806 1872
Size: 181 439 314 4545
Fragments arranged by size:
4545 439 314 181
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3583 4484 4746 820
Size: 197 926 1640 901 262 1553
Fragments arranged by size:
1640 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3270 493
Size: 2777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5479
SphI G_CATG'C
Cuts at: 1132 2837 1132
Size: 1705 3774
SrfI GCCC'GGGC
Cuts at: 2053 2053
Size: 5479
SspI AAT'ATT
Cuts at: 5 52 3403 5
Size: 47 3351 2081
Fragments arranged by size:
3351 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5479
StyI C'CwwG_G
Cuts at: 513 1065 2918 513
Size: 552 1853 3074
Fragments arranged by size:
3074 1853 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2594 2667 3506 3523 3676 3861 4306
Size: 1255 1014 73 839 17 153 185 445
Cuts at: 4306 325
Size: 1498
Fragments arranged by size:
1498 1255 1014 839 445 185 153 73
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 3725 5437
Size: 274 80 33 51 1443 234 1514 1712
Cuts at: 5437 96
Size: 138
Fragments arranged by size:
1712 1514 1443 274 234 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2443 2475 5439 5471 2443
Size: 32 2964 32 2451
Fragments arranged by size:
2964 2451 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5479
XbaI T'CTAG_A
Cuts at: 2815 2815
Size: 5479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2182 2182
Size: 5479
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5479
XmaI C'CCGG_G
Cuts at: 1870 2051 2490 2804 1870
Size: 181 439 314 4545
Fragments arranged by size:
4545 439 314 181
XmnI GAAnn'nnTTC
Cuts at: 2429 3608 2429
Size: 1179 4300
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3287 276
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SwaI