Restriction map for
pFN21AB5335
(Circular) (Six-base) MAPSORT of: pFN21AB5335.seq Check: 2218 from: 1 to: 6664
pFN21AB5335 6664 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4474 278
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4007 1937
Size: 2070 4594
Acc65I G'GTAC_C
Cuts at: 3985 3985
Size: 6664
AclI AA'CG_TT
Cuts at: 1563 4791 5164 1563
Size: 3228 373 3063
Fragments arranged by size:
3228 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2653 2737 2905 2989 3073 3157 3241 3409
Size: 1021 84 168 84 84 84 84 168
Cuts at: 3409 3493 3829 4727 6111 6379 1632
Size: 84 336 898 1384 268 1917
Fragments arranged by size:
1917 1384 1021 898 336 268 168 168
84 84 84 84 84 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6467
AflIII A'CryG_T
Cuts at: 1203 2727 2895 2979 3651 3830 5563 6540 1203
Size: 1524 168 84 672 179 1733 977 1327
Fragments arranged by size:
1733 1524 1327 977 672 179 168 84
AgeI A'CCGG_T
Cuts at: 5495 5495
Size: 6664
AhdI GACnn_n'nnGTC
Cuts at: 5393 5393
Size: 6664
AleI CACnn'nnGTG
Cuts at: 1166 2880 1166
Size: 1714 4950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6664
AlwNI CAG_nnn'CTG
Cuts at: 3075 4407 5979 3075
Size: 1332 1572 3760
Fragments arranged by size:
3760 1572 1332
ApaI G_GGCC'C
Cuts at: 2372 2372
Size: 6664
ApaLI G'TGCA_C
Cuts at: 4721 5877 4721
Size: 1156 5508
ApoI r'AATT_y
Cuts at: 1493 2127 2279 3265 3973 4199 4351 6460 1493
Size: 634 152 986 708 226 152 2109 1697
Fragments arranged by size:
2109 1697 986 708 634 226 152 152
AseI AT'TA_AT
Cuts at: 160 1032 5218 160
Size: 872 4186 1606
Fragments arranged by size:
4186 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
AvaI C'yCGr_G
Cuts at: 1870 1943 2794 3634 3989 1870
Size: 73 851 840 355 4545
Fragments arranged by size:
4545 851 840 355 73
BamHI G'GATC_C
Cuts at: 2362 3994 4361 2362
Size: 1632 367 4665
Fragments arranged by size:
4665 1632 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2450 3985 5440 618
Size: 325 217 627 663 1535 1455 1842
Fragments arranged by size:
1842 1535 1455 663 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2063 2238 2372 3715 3983 1391
Size: 672 175 134 1343 268 4072
Fragments arranged by size:
4072 1343 672 268 175 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3062 3884 928
Size: 971 1163 822 3708
Fragments arranged by size:
3708 1163 971 822
BbvCI CC'TCA_GC
Cuts at: 3863 3863
Size: 6664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4855 4889 1903
Size: 34 2918 34 3678
Fragments arranged by size:
3678 2918 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2697 4556 5766 2697
Size: 1859 1210 3595
Fragments arranged by size:
3595 1859 1210
BclI T'GATC_A
Cuts at: 1548 2422 1548
Size: 874 5790
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5275 136
Size: 107 122 71 631 605 3603 1525
Fragments arranged by size:
3603 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6659 6659
Size: 6664
BlpI GC'TnA_GC
Cuts at: 2057 4081 2057
Size: 2024 4640
Bme1580I G_kGCm'C
Cuts at: 1790 2372 3252 4725 5881 1790
Size: 582 880 1473 1156 2573
Fragments arranged by size:
2573 1473 1156 880 582
BmgBI CAC'GTC
Cuts at: 1318 3831 3885 1318
Size: 2513 54 4097
Fragments arranged by size:
4097 2513 54
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2132 3309 3680
Size: 416 53 83 399 24 715 1177 371
Cuts at: 3680 4395 5353 442
Size: 715 958 1753
Fragments arranged by size:
1753 1177 958 715 715 416 399 371
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6664
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2082 2082
Size: 6664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2183 2203 2254 2437 2485 5324 1239
Size: 878 66 20 51 183 48 2839 2579
Fragments arranged by size:
2839 2579 878 183 66 51 48 20
Bpu10I CC'TnA_GC
Cuts at: 3863 3921 3863
Size: 58 6606
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3332 3836 4789 5654 5952 3332
Size: 504 953 865 298 4044
Fragments arranged by size:
4044 953 865 504 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2122 2171 2404 3614 5327 882
Size: 617 623 49 233 1210 1713 2219
Fragments arranged by size:
2219 1713 1210 623 617 233 49
BsaAI yAC'GTr
Cuts at: 493 1204 2728 2980 4455 493
Size: 711 1524 252 1475 2702
Fragments arranged by size:
2702 1524 1475 711 252
BsaBI GATnn'nnATC
Cuts at: 6658 6658
Size: 6664
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4471 4853 5488 275
Size: 53 83 186 936 2938 382 635 1451
Fragments arranged by size:
2938 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2841 2879 5097 5495 5769 5916 6327 2841
Size: 38 2218 398 274 147 411 3178
Fragments arranged by size:
3178 2218 411 398 274 147 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3095 3125 3296 3326 3548
Size: 30 165 30 1692 30 171 30 222
Cuts at: 3548 3578 1178
Size: 30 4264
Fragments arranged by size:
4264 1692 222 171 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3065 1186
Size: 1879 4785
BseYI C'CCAG_C
Cuts at: 2157 2569 5867 6363 2157
Size: 412 3298 496 2458
Fragments arranged by size:
3298 2458 496 412
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2358 2836 2920 3088 3676 3760 3929
Size: 573 622 478 84 168 588 84 169
Cuts at: 3929 3951 1163
Size: 22 3876
Fragments arranged by size:
3876 622 588 573 478 169 168 84
84 22
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4114 4875 5903 1540
Size: 275 186 2113 761 1028 2301
Fragments arranged by size:
2301 2113 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2238 4725 4810 5881 2238
Size: 2487 85 1071 3021
Fragments arranged by size:
3021 2487 1071 85
BsmI GAATG_Cn'
Cuts at: 2009 4175 4268 2009
Size: 2166 93 4405
Fragments arranged by size:
4405 2166 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2238 2372 3252 3715 3983 4725
Size: 399 273 175 134 880 463 268 742
Cuts at: 4725 4810 5881 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 880 742 463 399 273 268
175 134 85
BspEI T'CCGG_A
Cuts at: 6327 6327
Size: 6664
BspHI T'CATG_A
Cuts at: 4553 6283 4553
Size: 1730 4934
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2694 4019 844
Size: 1850 1325 3489
Fragments arranged by size:
3489 1850 1325
BsrBI CCG'CTC
Cuts at: 1394 1823 4551 1394
Size: 429 2728 3507
Fragments arranged by size:
3507 2728 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5159 5333 65
Size: 1183 3911 174 1396
Fragments arranged by size:
3911 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2206 3605 5308 5495 1700
Size: 110 396 1399 1703 187 2869
Fragments arranged by size:
2869 1703 1399 396 187 110
BsrGI T'GTAC_A
Cuts at: 96 2079 96
Size: 1983 4681
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6664
BssSI C'ACGA_G
Cuts at: 4724 5736 4724
Size: 1012 5652
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2296 794
Size: 1502 5162
BstBI TT'CG_AA
Cuts at: 5468 5468
Size: 6664
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6664
BstYI r'GATC_y
Cuts at: 1972 2221 2319 2362 3994 4361 4746 4763 6215
Size: 249 98 43 1632 367 385 17 1452
Cuts at: 6215 6301 6313 6659 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1632 1452 385 367 346 249 98
86 43 17 12
Bsu36I CC'TnA_GG
Cuts at: 2439 2589 2439
Size: 150 6514
BtgI C'CryG_G
Cuts at: 513 1065 2004 3480 513
Size: 552 939 1476 3697
Fragments arranged by size:
3697 1476 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3181 487
Size: 683 56 1955 3970
Fragments arranged by size:
3970 1955 683 56
BtsI GCAGTG_nn'
Cuts at: 2908 3076 3668 4180 4974 4994 2908
Size: 168 592 512 794 20 4578
Fragments arranged by size:
4578 794 592 512 168 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3142 3177 3394 3429 3814 3849 529
Size: 35 2578 35 217 35 385 35 3344
Fragments arranged by size:
3344 2578 385 217 35 35 35 35
DraI TTT'AAA
Cuts at: 3968 3968
Size: 6664
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5671 809
Size: 507 4355 1802
Fragments arranged by size:
4355 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3415 3751 4111
Size: 54 1425 50 275 18 1585 336 360
Cuts at: 4111 5000 6629 8
Size: 889 1629 43
Fragments arranged by size:
1629 1585 1425 889 360 336 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4111 1537
Size: 275 2299 4090
Fragments arranged by size:
4090 2299 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2606 2759 3717 4594 1353
Size: 1253 153 958 877 3423
Fragments arranged by size:
3423 1253 958 877 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3304 5251 5625 5771 387
Size: 1064 138 1715 1947 374 146 1280
Fragments arranged by size:
1947 1715 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2236 2236
Size: 6664
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2183 2203 2254 2437 2485 2653
Size: 393 485 66 20 51 183 48 168
Cuts at: 2653 2737 2905 2989 3073 3157 3241 3409 3493
Size: 84 168 84 84 84 84 168 84
Cuts at: 3493 3829 4727 5324 6111 6379 1239
Size: 336 898 597 787 268 1524
Fragments arranged by size:
1524 898 787 597 485 393 336 268
183 168 168 168 84 84 84 84
84 84 66 51 48 20
EcoO109I rG'GnC_Cy
Cuts at: 2435 2435
Size: 6664
EcoRI G'AATT_C
Cuts at: 3265 3973 4351 3265
Size: 708 378 5578
Fragments arranged by size:
5578 708 378
FspI TGC'GCA
Cuts at: 5170 5170
Size: 6664
HaeII r_GCGC'y
Cuts at: 1678 5811 1678
Size: 4133 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5305 5337 5379 5411 1373
Size: 32 135 32 3733 32 42 32 2626
Fragments arranged by size:
3733 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2644 2850 3196 4008 4254 669
Size: 988 281 706 206 346 812 246 3079
Fragments arranged by size:
3079 988 812 706 346 281 246 206
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6664
HpaI GTT'AAC
Cuts at: 4254 4254
Size: 6664
KpnI G_GTAC'C
Cuts at: 3989 3989
Size: 6664
MfeI C'AATT_G
Cuts at: 2808 4263 2808
Size: 1455 5209
MluI A'CGCG_T
Cuts at: 6540 6540
Size: 6664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3462 5778 5962 1305
Size: 2157 2316 184 2007
Fragments arranged by size:
2316 2157 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3753 10
Size: 54 1425 343 1921 2921
Fragments arranged by size:
2921 1921 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2332 2880 3820 4622 4981 5140 518
Size: 648 1166 548 940 802 359 159 2042
Fragments arranged by size:
2042 1166 940 802 648 548 359 159
MspA1I CmG'CkG
Cuts at: 1606 2161 2701 3020 4080 4757 5905 6150 1606
Size: 555 540 319 1060 677 1148 245 2120
Fragments arranged by size:
2120 1148 1060 677 555 540 319 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3480 513
Size: 552 939 1476 3697
Fragments arranged by size:
3697 1476 939 552
NdeI CA'TA_TG
Cuts at: 387 3817 387
Size: 3430 3234
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6664
NotI GC'GGCC_GC
Cuts at: 4111 4111
Size: 6664
NspI r_CATG'y
Cuts at: 1132 1229 2297 2331 2899 3655 4022 5567 1132
Size: 97 1068 34 568 756 367 1545 2229
Fragments arranged by size:
2229 1545 1068 756 568 367 97 34
PasI CC'CwG_GG
Cuts at: 1348 2372 1348
Size: 1024 5640
PciI A'CATG_T
Cuts at: 2895 3651 5563 2895
Size: 756 1912 3996
Fragments arranged by size:
3996 1912 756
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6664
PmeI GTTT'AAAC
Cuts at: 3968 3968
Size: 6664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 4733 4765 6246 6278 3365
Size: 32 1336 32 1481 32 3751
Fragments arranged by size:
3751 1481 1336 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2435 2435
Size: 6664
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6664
PsiI TTA'TAA
Cuts at: 4234 4234
Size: 6664
PspOMI G'GGCC_C
Cuts at: 2368 2368
Size: 6664
PspXI vC'TCGA_Gb
Cuts at: 3634 3634
Size: 6664
PstI C_TGCA'G
Cuts at: 830 4016 830
Size: 3186 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6664
PvuII CAG'CTG
Cuts at: 2161 3020 2161
Size: 859 5805
SacI G_AGCT'C
Cuts at: 2238 2238
Size: 6664
SalI G'TCGA_C
Cuts at: 1936 4006 1936
Size: 2070 4594
SapI GCTCTTCn'nnn_
Cuts at: 2759 2759
Size: 6664
SbfI CC_TGCA'GG
Cuts at: 4016 4016
Size: 6664
ScaI AGT'ACT
Cuts at: 4912 6624 4912
Size: 1712 4952
SexAI A'CCwGG_T
Cuts at: 2112 2112
Size: 6664
SfcI C'TryA_G
Cuts at: 826 1045 2104 4012 5147 5828 6019 826
Size: 219 1059 1908 1135 681 191 1471
Fragments arranged by size:
1908 1471 1135 1059 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6664
SmaI CCC'GGG
Cuts at: 1872 3991 1872
Size: 2119 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3347 3634 3851 4768 5669 5931
Size: 197 926 1404 287 217 917 901 262
Cuts at: 5931 820
Size: 1553
Fragments arranged by size:
1553 1404 926 917 901 287 262 217
197
SnaBI TAC'GTA
Cuts at: 493 4455 493
Size: 3962 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6664
SphI G_CATG'C
Cuts at: 1132 4022 1132
Size: 2890 3774
SspI AAT'ATT
Cuts at: 5 52 4588 5
Size: 47 4536 2081
Fragments arranged by size:
4536 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6664
StyI C'CwwG_G
Cuts at: 513 1065 2004 3480 3754 4103 513
Size: 552 939 1476 274 349 3074
Fragments arranged by size:
3074 1476 939 552 349 274
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2051 2171 4691 4708 4861 5046 5491
Size: 1255 471 120 2520 17 153 185 445
Cuts at: 5491 325
Size: 1498
Fragments arranged by size:
2520 1498 1255 471 445 185 153 120
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2079 4910 6622
Size: 274 80 33 51 1443 102 2831 1712
Cuts at: 6622 96
Size: 138
Fragments arranged by size:
2831 1712 1443 274 138 102 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6624 6656 6624
Size: 32 6632
Tth111I GACn'n_nGTC
Cuts at: 1316 3883 1316
Size: 2567 4097
XbaI T'CTAG_A
Cuts at: 4000 4000
Size: 6664
XhoI C'TCGA_G
Cuts at: 1943 3634 1943
Size: 1691 4973
XmaI C'CCGG_G
Cuts at: 1870 3989 1870
Size: 2119 4545
XmnI GAAnn'nnTTC
Cuts at: 2750 4793 2750
Size: 2043 4621
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4472 276
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PsrI RsrII SacII SanDI SfiI
SfoI SrfI SwaI XcmI