Restriction map for
pFN21AB5332
(Circular) (Six-base) MAPSORT of: pFN21AB5332.seq Check: 9400 from: 1 to: 5530
pFN21AB5332 5530 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3340 278
Size: 53 83 186 936 1804 2468
Fragments arranged by size:
2468 1804 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2873 1937
Size: 936 4594
Acc65I G'GTAC_C
Cuts at: 2851 2851
Size: 5530
AclI AA'CG_TT
Cuts at: 1563 3657 4030 1563
Size: 2094 373 3063
Fragments arranged by size:
3063 2094 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3593 4977 5245 1632
Size: 1961 1384 268 1917
Fragments arranged by size:
1961 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5333
AflIII A'CryG_T
Cuts at: 1203 2270 2344 4429 5406 1203
Size: 1067 74 2085 977 1327
Fragments arranged by size:
2085 1327 1067 977 74
AgeI A'CCGG_T
Cuts at: 4361 4361
Size: 5530
AhdI GACnn_n'nnGTC
Cuts at: 4259 4259
Size: 5530
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5530
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5530
AlwNI CAG_nnn'CTG
Cuts at: 2479 3273 4845 2479
Size: 794 1572 3164
Fragments arranged by size:
3164 1572 794
ApaLI G'TGCA_C
Cuts at: 3587 4743 3587
Size: 1156 4374
ApoI r'AATT_y
Cuts at: 1493 2189 2839 3065 3217 5326 1493
Size: 696 650 226 152 2109 1697
Fragments arranged by size:
2109 1697 696 650 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2213 4084 160
Size: 872 1181 1871 1606
Fragments arranged by size:
1871 1606 1181 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
AvaI C'yCGr_G
Cuts at: 1870 1943 2259 2288 2355 2379 2603 2855 1870
Size: 73 316 29 67 24 224 252 4545
Fragments arranged by size:
4545 316 252 224 73 67 29 24
BamHI G'GATC_C
Cuts at: 2860 3227 2860
Size: 367 5163
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2851 4306 618
Size: 325 217 627 1064 1455 1842
Fragments arranged by size:
1842 1455 1064 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2046 2849 1391
Size: 655 803 4072
Fragments arranged by size:
4072 803 655
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2107 2337 928
Size: 971 208 230 4121
Fragments arranged by size:
4121 971 230 208
BbvCI CC'TCA_GC
Cuts at: 2124 2124
Size: 5530
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2240 2274 3721 3755 1903
Size: 34 50 34 219 34 1447 34 3678
Fragments arranged by size:
3678 1447 219 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3422 4632 3422
Size: 1210 4320
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5530
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4141 136
Size: 107 122 71 631 605 2469 1525
Fragments arranged by size:
2469 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5525 5525
Size: 5530
BlpI GC'TnA_GC
Cuts at: 2947 2947
Size: 5530
Bme1580I G_kGCm'C
Cuts at: 1790 3591 4747 1790
Size: 1801 1156 2573
Fragments arranged by size:
2573 1801 1156
BmgBI CAC'GTC
Cuts at: 1318 2352 1318
Size: 1034 4496
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2158 3261 4219
Size: 416 53 83 399 24 741 1103 958
Cuts at: 4219 442
Size: 1753
Fragments arranged by size:
1753 1103 958 741 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4190 1239
Size: 2951 2579
Bpu10I CC'TnA_GC
Cuts at: 2124 2124
Size: 5530
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3655 4520 4818 3655
Size: 865 298 4367
Fragments arranged by size:
4367 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2352 4193 882
Size: 617 853 1841 2219
Fragments arranged by size:
2219 1841 853 617
BsaAI yAC'GTr
Cuts at: 493 1204 2431 3321 493
Size: 711 1227 890 2702
Fragments arranged by size:
2702 1227 890 711
BsaBI GATnn'nnATC
Cuts at: 5524 5524
Size: 5530
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3337 3719 4354 275
Size: 53 83 186 936 1804 382 635 1451
Fragments arranged by size:
1804 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2389 3963 4361 4635 4782 5193 2389
Size: 1574 398 274 147 411 2726
Fragments arranged by size:
2726 1574 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2351 2381 1178
Size: 30 165 30 948 30 4327
Fragments arranged by size:
4327 948 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 1186
Size: 1105 4425
BseYI C'CCAG_C
Cuts at: 4733 5229 4733
Size: 496 5034
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2251 1163
Size: 573 515 4442
Fragments arranged by size:
4442 573 515
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2980 3741 4769 1540
Size: 275 186 979 761 1028 2301
Fragments arranged by size:
2301 1028 979 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2297 3591 3676 4747 2046
Size: 251 1294 85 1071 2829
Fragments arranged by size:
2829 1294 1071 251 85
BsiWI C'GTAC_G
Cuts at: 2010 2010
Size: 5530
BsmI GAATG_Cn'
Cuts at: 3041 3134 3041
Size: 93 5437
BsmBI CGTCTCn'nnnn_
Cuts at: 2358 2358
Size: 5530
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2297 2849 3591 3676 4747 1391
Size: 399 256 251 552 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 552 399 256 251 85
BspEI T'CCGG_A
Cuts at: 5193 5193
Size: 5530
BspHI T'CATG_A
Cuts at: 2182 3419 5149 2182
Size: 1237 1730 2563
Fragments arranged by size:
2563 1730 1237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2885 844
Size: 2041 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3417 1394
Size: 429 1594 3507
Fragments arranged by size:
3507 1594 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2193 2332 4025 4199 65
Size: 1183 945 139 1693 174 1396
Fragments arranged by size:
1693 1396 1183 945 174 139
BsrFI r'CCGG_y
Cuts at: 1700 1810 4174 4361 1700
Size: 110 2364 187 2869
Fragments arranged by size:
2869 2364 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5530
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5530
BssSI C'ACGA_G
Cuts at: 2798 3590 4602 2798
Size: 792 1012 3726
Fragments arranged by size:
3726 1012 792
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5530
BstBI TT'CG_AA
Cuts at: 4334 4334
Size: 5530
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5530
BstYI r'GATC_y
Cuts at: 1972 2802 2860 3227 3612 3629 5081 5167 5179
Size: 830 58 367 385 17 1452 86 12
Cuts at: 5179 5525 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 830 385 367 346 86 58
17 12
Bsu36I CC'TnA_GG
Cuts at: 2579 2579
Size: 5530
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4978
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2104 487
Size: 683 56 878 3913
Fragments arranged by size:
3913 878 683 56
BtsI GCAGTG_nn'
Cuts at: 3046 3840 3860 3046
Size: 794 20 4716
Fragments arranged by size:
4716 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5530
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5495
DraI TTT'AAA
Cuts at: 2834 2834
Size: 5530
DraIII CAC_nnn'GTG
Cuts at: 2590 2590
Size: 5530
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2057 4537 809
Size: 507 741 2480 1802
Fragments arranged by size:
2480 1802 741 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2612 2977
Size: 54 1425 50 275 18 264 518 365
Cuts at: 2977 3866 5495 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 518 365 275 264 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2977 1537
Size: 275 1165 4090
Fragments arranged by size:
4090 1165 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3460 1353
Size: 2107 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4117 4491 4637 387
Size: 1064 138 2528 374 146 1280
Fragments arranged by size:
2528 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2044 2044
Size: 5530
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3593 4190 4977 5245 1239
Size: 393 1961 597 787 268 1524
Fragments arranged by size:
1961 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2541 2541
Size: 5530
EcoRI G'AATT_C
Cuts at: 2189 2839 3217 2189
Size: 650 378 4502
Fragments arranged by size:
4502 650 378
FspI TGC'GCA
Cuts at: 4036 4036
Size: 5530
HaeII r_GCGC'y
Cuts at: 1678 4677 1678
Size: 2999 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2349 2351 2381 2383 2633
Size: 32 135 32 777 2 30 2 250
Cuts at: 2633 2665 4171 4203 4245 4277 1373
Size: 32 1506 32 42 32 2626
Fragments arranged by size:
2626 1506 777 250 135 42 32 32
32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2874 3120 669
Size: 988 281 936 246 3079
Fragments arranged by size:
3079 988 936 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5530
HpaI GTT'AAC
Cuts at: 3120 3120
Size: 5530
KpnI G_GTAC'C
Cuts at: 2855 2855
Size: 5530
MfeI C'AATT_G
Cuts at: 3129 3129
Size: 5530
MluI A'CGCG_T
Cuts at: 5406 5406
Size: 5530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2394 4644 4828 1305
Size: 1089 2250 184 2007
Fragments arranged by size:
2250 2007 1089 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 2614 10
Size: 54 1425 343 264 518 2926
Fragments arranged by size:
2926 1425 518 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 3488 3847 4006 518
Size: 648 1224 1098 359 159 2042
Fragments arranged by size:
2042 1224 1098 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2946 3623 4771 5016 1606
Size: 1340 677 1148 245 2120
Fragments arranged by size:
2120 1340 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4978
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5530
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5530
NotI GC'GGCC_GC
Cuts at: 2977 2977
Size: 5530
NspI r_CATG'y
Cuts at: 1132 1229 2274 2348 2525 2888 4433 1132
Size: 97 1045 74 177 363 1545 2229
Fragments arranged by size:
2229 1545 1045 363 177 97 74
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5530
PciI A'CATG_T
Cuts at: 2270 2344 4429 2270
Size: 74 2085 3371
Fragments arranged by size:
3371 2085 74
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2148 1108
Size: 1040 4490
PfoI T'CCnGG_A
Cuts at: 2399 2399
Size: 5530
PmeI GTTT'AAAC
Cuts at: 2834 2834
Size: 5530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 5112 5144 3599
Size: 32 1481 32 3985
Fragments arranged by size:
3985 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5530
PsiI TTA'TAA
Cuts at: 3100 3100
Size: 5530
PstI C_TGCA'G
Cuts at: 830 2253 2545 2882 830
Size: 1423 292 337 3478
Fragments arranged by size:
3478 1423 337 292
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5530
SacI G_AGCT'C
Cuts at: 2046 2046
Size: 5530
SalI G'TCGA_C
Cuts at: 1936 2872 1936
Size: 936 4594
SbfI CC_TGCA'GG
Cuts at: 2882 2882
Size: 5530
ScaI AGT'ACT
Cuts at: 2255 2627 3778 5490 2255
Size: 372 1151 1712 2295
Fragments arranged by size:
2295 1712 1151 372
SfcI C'TryA_G
Cuts at: 826 1045 2249 2541 2878 4013 4694 4885 826
Size: 219 1204 292 337 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 681 337 292 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5530
SmaI CCC'GGG
Cuts at: 1872 2381 2857 1872
Size: 509 476 4545
Fragments arranged by size:
4545 509 476
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2355 3634 4535 4797 820
Size: 197 926 412 1279 901 262 1553
Fragments arranged by size:
1553 1279 926 901 412 262 197
SnaBI TAC'GTA
Cuts at: 493 3321 493
Size: 2828 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5530
SphI G_CATG'C
Cuts at: 1132 2525 2888 1132
Size: 1393 363 3774
Fragments arranged by size:
3774 1393 363
SspI AAT'ATT
Cuts at: 5 52 3454 5
Size: 47 3402 2081
Fragments arranged by size:
3402 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5530
StyI C'CwwG_G
Cuts at: 513 1065 2969 513
Size: 552 1904 3074
Fragments arranged by size:
3074 1904 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3557 3574 3727 3912 4357 325
Size: 1255 1977 17 153 185 445 1498
Fragments arranged by size:
1977 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 2625 3776
Size: 274 80 33 51 1443 276 372 1151
Cuts at: 3776 5488 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1151 372 276 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5490 5522 5490
Size: 32 5498
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5530
XbaI T'CTAG_A
Cuts at: 2866 2866
Size: 5530
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2428 2428
Size: 5530
XhoI C'TCGA_G
Cuts at: 1943 2355 1943
Size: 412 5118
XmaI C'CCGG_G
Cuts at: 1870 2379 2855 1870
Size: 509 476 4545
Fragments arranged by size:
4545 509 476
XmnI GAAnn'nnTTC
Cuts at: 3659 3659
Size: 5530
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3338 276
Size: 53 83 186 936 1804 2468
Fragments arranged by size:
2468 1804 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BstXI BstZ17I EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI