Restriction map for
pFN21AB5319
(Circular) (Six-base) MAPSORT of: pFN21AB5319.seq Check: 4434 from: 1 to: 5983
pFN21AB5319 5983 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2712 3326 1937
Size: 775 614 4594
Fragments arranged by size:
4594 775 614
Acc65I G'GTAC_C
Cuts at: 3304 3304
Size: 5983
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2147 2279 4046 5430 5698 1632
Size: 515 132 1767 1384 268 1917
Fragments arranged by size:
1917 1767 1384 515 268 132
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5786
AflIII A'CryG_T
Cuts at: 1203 4882 5859 1203
Size: 3679 977 1327
Fragments arranged by size:
3679 1327 977
AgeI A'CCGG_T
Cuts at: 4814 4814
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 4712 4712
Size: 5983
AleI CACnn'nnGTG
Cuts at: 1166 2765 1166
Size: 1599 4384
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2966 1184
Size: 1782 4201
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2871 2903 2871
Size: 32 5951
AlwNI CAG_nnn'CTG
Cuts at: 3226 3726 5298 3226
Size: 500 1572 3911
Fragments arranged by size:
3911 1572 500
ApaLI G'TGCA_C
Cuts at: 4040 5196 4040
Size: 1156 4827
ApoI r'AATT_y
Cuts at: 1493 2845 3030 3047 3292 3518 3670 5779 1493
Size: 1352 185 17 245 226 152 2109 1697
Fragments arranged by size:
2109 1697 1352 245 226 185 152 17
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 3308 1870
Size: 73 1365 4545
Fragments arranged by size:
4545 1365 73
BamHI G'GATC_C
Cuts at: 3313 3680 3313
Size: 367 5616
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 3304 4759 618
Size: 325 217 627 354 1163 1455 1842
Fragments arranged by size:
1842 1455 1163 627 354 325 217
BanII G_rGCy'C
Cuts at: 1391 2724 3302 1391
Size: 1333 578 4072
Fragments arranged by size:
4072 1333 578
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5012
BbvCI CC'TCA_GC
Cuts at: 2448 2448
Size: 5983
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4174 4208 1903
Size: 34 2237 34 3678
Fragments arranged by size:
3678 2237 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5085 3875
Size: 1210 4773
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4594 136
Size: 107 122 71 631 605 2922 1525
Fragments arranged by size:
2922 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5978 5978
Size: 5983
BlpI GC'TnA_GC
Cuts at: 3400 3400
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 1790 4044 5200 1790
Size: 2254 1156 2573
Fragments arranged by size:
2573 2254 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3714 4672 442
Size: 416 53 83 399 24 2297 958 1753
Fragments arranged by size:
2297 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3203 4643 1239
Size: 1964 1440 2579
Fragments arranged by size:
2579 1964 1440
Bpu10I CC'TnA_GC
Cuts at: 2448 2448
Size: 5983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2188 4108 4973 5271 2188
Size: 1920 865 298 2900
Fragments arranged by size:
2900 1920 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4646 882
Size: 617 3147 2219
Fragments arranged by size:
3147 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3125 3774 493
Size: 711 1921 649 2702
Fragments arranged by size:
2702 1921 711 649
BsaBI GATnn'nnATC
Cuts at: 5977 5977
Size: 5983
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3790 4172 4807 275
Size: 53 83 186 936 2257 382 635 1451
Fragments arranged by size:
2257 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2241 2841 4416 4814 5088 5235 5646 2241
Size: 600 1575 398 274 147 411 2578
Fragments arranged by size:
2578 1575 600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2898 2928 1178
Size: 30 165 30 1495 30 4233
Fragments arranged by size:
4233 1495 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2746 1186
Size: 1560 4423
BseYI C'CCAG_C
Cuts at: 3144 5186 5682 3144
Size: 2042 496 3445
Fragments arranged by size:
3445 2042 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3191 1163
Size: 573 1455 3955
Fragments arranged by size:
3955 1455 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3433 4194 5222 1540
Size: 275 186 1432 761 1028 2301
Fragments arranged by size:
2301 1432 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2705 2724 4044 4129 5200 2705
Size: 19 1320 85 1071 3488
Fragments arranged by size:
3488 1320 1071 85 19
BsmI GAATG_Cn'
Cuts at: 2330 2930 3494 3587 2330
Size: 600 564 93 4726
Fragments arranged by size:
4726 600 564 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2705 2724 3302 4044 4129 5200 1391
Size: 399 915 19 578 742 85 1071 2174
Fragments arranged by size:
2174 1071 915 742 578 399 85 19
BspEI T'CCGG_A
Cuts at: 5646 5646
Size: 5983
BspHI T'CATG_A
Cuts at: 3872 5602 3872
Size: 1730 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3338 844
Size: 2494 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2211 3870 1394
Size: 429 388 1659 3507
Fragments arranged by size:
3507 1659 429 388
BsrDI GCAATG_nn'
Cuts at: 65 1248 4478 4652 65
Size: 1183 3230 174 1396
Fragments arranged by size:
3230 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4627 4814 1700
Size: 110 2817 187 2869
Fragments arranged by size:
2869 2817 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5983
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 4043 5055 4043
Size: 1012 4971
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3186 794
Size: 2392 3591
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5983
BstXI CCAn_nnnn'nTGG
Cuts at: 2697 2697
Size: 5983
BstYI r'GATC_y
Cuts at: 1972 3006 3313 3680 4065 4082 5534 5620 5632
Size: 1034 307 367 385 17 1452 86 12
Cuts at: 5632 5978 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1034 385 367 346 307 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2145 3079 513
Size: 552 1080 934 3417
Fragments arranged by size:
3417 1080 934 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2088 487
Size: 683 56 862 4382
Fragments arranged by size:
4382 862 683 56
BtsI GCAGTG_nn'
Cuts at: 3499 4293 4313 3499
Size: 794 20 5169
Fragments arranged by size:
5169 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5948
DraI TTT'AAA
Cuts at: 3287 3287
Size: 5983
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4990 809
Size: 507 3674 1802
Fragments arranged by size:
3674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3430 4319 5948
Size: 54 1425 50 275 18 1600 889 1629
Cuts at: 5948 8
Size: 43
Fragments arranged by size:
1629 1600 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3430 1537
Size: 275 1618 4090
Fragments arranged by size:
4090 1618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 3913 1353
Size: 689 1871 3423
Fragments arranged by size:
3423 1871 689
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4570 4944 5090 387
Size: 1064 138 2981 374 146 1280
Fragments arranged by size:
2981 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2722 2722
Size: 5983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2279 3203 4046 4643 5430 5698
Size: 393 515 132 924 843 597 787 268
Cuts at: 5698 1239
Size: 1524
Fragments arranged by size:
1524 924 843 787 597 515 393 268
132
EcoRI G'AATT_C
Cuts at: 2845 3292 3670 2845
Size: 447 378 5158
Fragments arranged by size:
5158 447 378
FspI TGC'GCA
Cuts at: 4489 4489
Size: 5983
HaeII r_GCGC'y
Cuts at: 1678 2284 5130 1678
Size: 606 2846 2531
Fragments arranged by size:
2846 2531 606
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4624 4656 4698 4730 1373
Size: 32 135 32 3052 32 42 32 2626
Fragments arranged by size:
3052 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2839 3327 3573 669
Size: 988 281 901 488 246 3079
Fragments arranged by size:
3079 988 901 488 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5983
HpaI GTT'AAC
Cuts at: 2839 3573 2839
Size: 734 5249
KpnI G_GTAC'C
Cuts at: 3308 3308
Size: 5983
MfeI C'AATT_G
Cuts at: 3582 3582
Size: 5983
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2820 2884 2939 5097 5281 1305
Size: 1515 64 55 2158 184 2007
Fragments arranged by size:
2158 2007 1515 184 64 55
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4161
Fragments arranged by size:
4161 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2323 2765 2948 3941 4300 4459
Size: 648 843 314 442 183 993 359 159
Cuts at: 4459 518
Size: 2042
Fragments arranged by size:
2042 993 843 648 442 359 314 183
159
MspA1I CmG'CkG
Cuts at: 1606 2042 3399 4076 5224 5469 1606
Size: 436 1357 677 1148 245 2120
Fragments arranged by size:
2120 1357 1148 677 436 245
NcoI C'CATG_G
Cuts at: 513 1065 2145 3079 513
Size: 552 1080 934 3417
Fragments arranged by size:
3417 1080 934 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5983
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 2503 3341 4886 1132
Size: 97 1274 838 1545 2229
Fragments arranged by size:
2229 1545 1274 838 97
PasI CC'CwG_GG
Cuts at: 1348 2177 1348
Size: 829 5154
PciI A'CATG_T
Cuts at: 4882 4882
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3226 1108
Size: 2118 3865
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4052 4084 5565 5597 4052
Size: 32 1481 32 4438
Fragments arranged by size:
4438 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5983
PsiI TTA'TAA
Cuts at: 3553 3553
Size: 5983
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2954 2986 2954
Size: 32 5951
PstI C_TGCA'G
Cuts at: 830 3335 830
Size: 2505 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
PvuII CAG'CTG
Cuts at: 2042 2042
Size: 5983
SacI G_AGCT'C
Cuts at: 2724 2724
Size: 5983
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SbfI CC_TGCA'GG
Cuts at: 3335 3335
Size: 5983
ScaI AGT'ACT
Cuts at: 4231 5943 4231
Size: 1712 4271
SfcI C'TryA_G
Cuts at: 826 1045 3331 4466 5147 5338 826
Size: 219 2286 1135 681 191 1471
Fragments arranged by size:
2286 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5983
SmaI CCC'GGG
Cuts at: 1872 3310 1872
Size: 1438 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2167 4087 4988 5250 820
Size: 197 926 224 1920 901 262 1553
Fragments arranged by size:
1920 1553 926 901 262 224 197
SnaBI TAC'GTA
Cuts at: 493 3125 3774 493
Size: 2632 649 2702
Fragments arranged by size:
2702 2632 649
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5983
SphI G_CATG'C
Cuts at: 1132 3341 1132
Size: 2209 3774
SspI AAT'ATT
Cuts at: 5 52 3907 5
Size: 47 3855 2081
Fragments arranged by size:
3855 2081 47
StuI AGG'CCT
Cuts at: 1517 2165 1517
Size: 648 5335
StyI C'CwwG_G
Cuts at: 513 1065 2145 2818 3079 3422 513
Size: 552 1080 673 261 343 3074
Fragments arranged by size:
3074 1080 673 552 343 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2049 2726 3006 4010 4027 4180 4365
Size: 1255 469 677 280 1004 17 153 185
Cuts at: 4365 4810 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1004 677 469 445 280 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2404 3116 4229
Size: 274 80 33 51 1443 427 712 1113
Cuts at: 4229 5941 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1113 712 427 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2898 2930 5943 5975 2898
Size: 32 3013 32 2906
Fragments arranged by size:
3013 2906 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5983
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2152 2767 2152
Size: 615 5368
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 3308 1870
Size: 1438 4545
XmnI GAAnn'nnTTC
Cuts at: 2810 4112 2810
Size: 1302 4681
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI