Restriction map for
pFN21AB5318
(Circular) (Six-base) MAPSORT of: pFN21AB5318.seq Check: 9961 from: 1 to: 5980
pFN21AB5318 5980 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2718 2718
Size: 5980
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3323 1937
Size: 1386 4594
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2105 2175 2190 4043 5427 5695 1632
Size: 473 70 15 1853 1384 268 1917
Fragments arranged by size:
1917 1853 1384 473 268 70 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 2773 4709 2773
Size: 1936 4044
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5980
AlwNI CAG_nnn'CTG
Cuts at: 3182 3723 5295 3182
Size: 541 1572 3867
Fragments arranged by size:
3867 1572 541
ApaLI G'TGCA_C
Cuts at: 4037 5193 4037
Size: 1156 4824
ApoI r'AATT_y
Cuts at: 1493 2312 2557 2563 2858 2958 3289 3515 3667
Size: 819 245 6 295 100 331 226 152
Cuts at: 3667 5776 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 819 331 295 245 226 152
100 6
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 2247 3305 1870
Size: 73 304 1058 4545
Fragments arranged by size:
4545 1058 304 73
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2706 3301 4756 618
Size: 325 217 627 919 595 1455 1842
Fragments arranged by size:
1842 1455 919 627 595 325 217
BanII G_rGCy'C
Cuts at: 1391 3299 1391
Size: 1908 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5009
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4171 4205 1903
Size: 34 2234 34 3678
Fragments arranged by size:
3678 2234 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3872 5082 3872
Size: 1210 4770
BclI T'GATC_A
Cuts at: 1548 2746 1548
Size: 1198 4782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4591 136
Size: 107 122 71 631 605 2919 1525
Fragments arranged by size:
2919 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5975 5975
Size: 5980
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 4041 5197 1790
Size: 2251 1156 2573
Fragments arranged by size:
2573 2251 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2124 3711 4669
Size: 416 53 83 399 24 707 1587 958
Cuts at: 4669 442
Size: 1753
Fragments arranged by size:
1753 1587 958 707 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3075 3284 4640 1239
Size: 1836 209 1356 2579
Fragments arranged by size:
2579 1836 1356 209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4105 4970 5268 4105
Size: 865 298 4817
Fragments arranged by size:
4817 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2552 2982 4643 882
Size: 617 1053 430 1661 2219
Fragments arranged by size:
2219 1661 1053 617 430
BsaAI yAC'GTr
Cuts at: 493 1204 3771 493
Size: 711 2567 2702
Fragments arranged by size:
2702 2567 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5755
Fragments arranged by size:
5755 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 1186
Size: 868 5112
BseYI C'CCAG_C
Cuts at: 2203 5183 5679 2203
Size: 2980 496 2504
Fragments arranged by size:
2980 2504 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2699 1163
Size: 573 963 4444
Fragments arranged by size:
4444 963 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2392 4041 4126 5197 2392
Size: 1649 85 1071 3175
Fragments arranged by size:
3175 1649 1071 85
BsmI GAATG_Cn'
Cuts at: 3491 3584 3491
Size: 93 5887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2392 3299 4041 4126 5197 1391
Size: 399 602 907 742 85 1071 2174
Fragments arranged by size:
2174 1071 907 742 602 399 85
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2718 3335 844
Size: 1874 617 3489
Fragments arranged by size:
3489 1874 617
BsrBI CCG'CTC
Cuts at: 1394 1823 3867 1394
Size: 429 2044 3507
Fragments arranged by size:
3507 2044 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2418 4475 4649 65
Size: 1183 1170 2057 174 1396
Fragments arranged by size:
2057 1396 1183 1170 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2873 4624 4811 1700
Size: 110 1063 1751 187 2869
Fragments arranged by size:
2869 1751 1063 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 2717 4040 5052 2717
Size: 1323 1012 3645
Fragments arranged by size:
3645 1323 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2713 3074 794
Size: 1919 361 3700
Fragments arranged by size:
3700 1919 361
BstBI TT'CG_AA
Cuts at: 2940 4784 2940
Size: 1844 4136
BstEII G'GTnAC_C
Cuts at: 1184 2525 1184
Size: 1341 4639
BstYI r'GATC_y
Cuts at: 1972 2732 2905 3275 3310 3677 4062 4079 5531
Size: 760 173 370 35 367 385 17 1452
Cuts at: 5531 5617 5629 5975 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 760 385 370 367 346 173
86 35 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5241
Fragments arranged by size:
5241 683 56
BtsI GCAGTG_nn'
Cuts at: 2817 3120 3496 4290 4310 2817
Size: 303 376 794 20 4487
Fragments arranged by size:
4487 794 376 303 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DraIII CAC_nnn'GTG
Cuts at: 2806 2806
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2215 4987 809
Size: 507 899 2772 1802
Fragments arranged by size:
2772 1802 899 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2674 3427 4316
Size: 54 1425 50 275 18 844 753 889
Cuts at: 4316 5945 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 844 753 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2124 3231 3910 1353
Size: 771 1107 679 3423
Fragments arranged by size:
3423 1107 771 679
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4567 4941 5087 387
Size: 1064 138 2978 374 146 1280
Fragments arranged by size:
2978 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2175 2190 3075 3284 4043 4640
Size: 393 473 70 15 885 209 759 597
Cuts at: 4640 5427 5695 1239
Size: 787 268 1524
Fragments arranged by size:
1524 885 787 759 597 473 393 268
209 70 15
EcoRI G'AATT_C
Cuts at: 2557 3289 3667 2557
Size: 732 378 4870
Fragments arranged by size:
4870 732 378
FspI TGC'GCA
Cuts at: 2814 4486 2814
Size: 1672 4308
FspAI rTGC'GCAy
Cuts at: 2814 2814
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 5127 1678
Size: 3449 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2101 2133 2560 2592 2759
Size: 32 135 32 529 32 427 32 167
Cuts at: 2759 2791 3224 3256 4621 4653 4695 4727 1373
Size: 32 433 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1365 529 433 427 167 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2830 3324 3570 669
Size: 988 281 892 494 246 3079
Fragments arranged by size:
3079 988 892 494 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5094 5278 1305
Size: 3789 184 2007
Fragments arranged by size:
3789 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2676 10
Size: 54 1425 343 844 3314
Fragments arranged by size:
3314 1425 844 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2952 3938 4297 4456 518
Size: 648 1786 986 359 159 2042
Fragments arranged by size:
2042 1786 986 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2684 3396 4073 5221 5466 1606
Size: 1078 712 677 1148 245 2120
Fragments arranged by size:
2120 1148 1078 712 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 2401 3338 4883 1132
Size: 97 1172 937 1545 2229
Fragments arranged by size:
2229 1545 1172 937 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5980
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5980
PfoI T'CCnGG_A
Cuts at: 2552 2552
Size: 5980
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3550 3550
Size: 5980
PspXI vC'TCGA_Gb
Cuts at: 2247 2247
Size: 5980
PstI C_TGCA'G
Cuts at: 830 3332 830
Size: 2502 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
PvuII CAG'CTG
Cuts at: 2684 2684
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SapI GCTCTTCn'nnn_
Cuts at: 2124 2124
Size: 5980
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SexAI A'CCwGG_T
Cuts at: 2100 2771 2100
Size: 671 5309
SfcI C'TryA_G
Cuts at: 826 1045 2851 3328 4463 5144 5335 826
Size: 219 1806 477 1135 681 191 1471
Fragments arranged by size:
1806 1471 1135 681 477 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 3307 1872
Size: 1435 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2247 4084 4985 5247 820
Size: 197 926 304 1837 901 262 1553
Fragments arranged by size:
1837 1553 926 901 304 262 197
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 3223 3904 5
Size: 47 3171 681 2081
Fragments arranged by size:
3171 2081 681 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2004 3161 3419 513
Size: 552 939 1157 258 3074
Fragments arranged by size:
3074 1157 939 552 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4007 4024 4177 4362 4807 325
Size: 1255 2427 17 153 185 445 1498
Fragments arranged by size:
2427 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2251 4226 5938
Size: 274 80 33 51 1443 274 1975 1712
Cuts at: 5938 96
Size: 138
Fragments arranged by size:
1975 1712 1443 274 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5940 5972 5940
Size: 32 5948
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 2247 1943
Size: 304 5676
XmaI C'CCGG_G
Cuts at: 1870 3305 1870
Size: 1435 4545
XmnI GAAnn'nnTTC
Cuts at: 2862 2994 4109 2862
Size: 132 1115 4733
Fragments arranged by size:
4733 1115 132
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI XcmI