Restriction map for
pFN21AB5305
(Circular) (Six-base) MAPSORT of: pFN21AB5305.seq Check: 34 from: 1 to: 6268
pFN21AB5305 6268 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3032 3299 3032
Size: 267 6001
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4078 278
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3327 3611 1937
Size: 1390 284 4594
Fragments arranged by size:
4594 1390 284
Acc65I G'GTAC_C
Cuts at: 3589 3589
Size: 6268
AclI AA'CG_TT
Cuts at: 1563 4395 4768 1563
Size: 2832 373 3063
Fragments arranged by size:
3063 2832 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2985 3476 3585 4331 5715 5983 1632
Size: 1353 491 109 746 1384 268 1917
Fragments arranged by size:
1917 1384 1353 746 491 268 109
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6071
AflIII A'CryG_T
Cuts at: 1203 5167 6144 1203
Size: 3964 977 1327
Fragments arranged by size:
3964 1327 977
AgeI A'CCGG_T
Cuts at: 5099 5099
Size: 6268
AhdI GACnn_n'nnGTC
Cuts at: 4997 4997
Size: 6268
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6268
AlwNI CAG_nnn'CTG
Cuts at: 4011 5583 4011
Size: 1572 4696
ApaLI G'TGCA_C
Cuts at: 4325 5481 4325
Size: 1156 5112
ApoI r'AATT_y
Cuts at: 1493 3241 3577 3803 3955 6064 1493
Size: 1748 336 226 152 2109 1697
Fragments arranged by size:
2109 1748 1697 336 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2196 4822 160
Size: 872 1164 2626 1606
Fragments arranged by size:
2626 1606 1164 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
AvaI C'yCGr_G
Cuts at: 1870 1943 3593 1870
Size: 73 1650 4545
Fragments arranged by size:
4545 1650 73
BamHI G'GATC_C
Cuts at: 3598 3965 3598
Size: 367 5901
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3017 3404 3589 5044 618
Size: 325 217 627 1230 387 185 1455 1842
Fragments arranged by size:
1842 1455 1230 627 387 325 217 185
BanII G_rGCy'C
Cuts at: 1391 2290 3587 1391
Size: 899 1297 4072
Fragments arranged by size:
4072 1297 899
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3464 928
Size: 971 1565 3732
Fragments arranged by size:
3732 1565 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2045 2079 4459 4493 1903
Size: 34 108 34 2380 34 3678
Fragments arranged by size:
3678 2380 108 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4160 5370 4160
Size: 1210 5058
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6268
BfrBI ATG'CAT
Cuts at: 3161 3161
Size: 6268
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3477 4879 136
Size: 107 122 71 631 605 1805 1402 1525
Fragments arranged by size:
1805 1525 1402 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3426 6263 3426
Size: 2837 3431
BlpI GC'TnA_GC
Cuts at: 3685 3685
Size: 6268
Bme1580I G_kGCm'C
Cuts at: 1790 3020 4329 5485 1790
Size: 1230 1309 1156 2573
Fragments arranged by size:
2573 1309 1230 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6268
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3156 3999 4957
Size: 416 53 83 399 24 1739 843 958
Cuts at: 4957 442
Size: 1753
Fragments arranged by size:
1753 1739 958 843 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2229 4928 1239
Size: 990 2699 2579
Fragments arranged by size:
2699 2579 990
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3048 4393 5258 5556 3048
Size: 1345 865 298 3760
Fragments arranged by size:
3760 1345 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4931 882
Size: 617 3432 2219
Fragments arranged by size:
3432 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4059 493
Size: 711 2855 2702
Fragments arranged by size:
2855 2702 711
BsaBI GATnn'nnATC
Cuts at: 6262 6262
Size: 6268
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4075 4457 5092 275
Size: 53 83 186 936 2542 382 635 1451
Fragments arranged by size:
2542 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2068 2655 4701 5099 5373 5520 5931 2068
Size: 587 2046 398 274 147 411 2405
Fragments arranged by size:
2405 2046 587 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2823 2853 1178
Size: 30 165 30 1420 30 4593
Fragments arranged by size:
4593 1420 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3083 1186
Size: 1897 4371
BseYI C'CCAG_C
Cuts at: 5471 5967 5471
Size: 496 5772
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2627 1163
Size: 573 891 4804
Fragments arranged by size:
4804 891 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2073 3718 4479 5507 1540
Size: 275 186 72 1645 761 1028 2301
Fragments arranged by size:
2301 1645 1028 761 275 186 72
BsiHKAI G_wGCw'C
Cuts at: 4329 4414 5485 4329
Size: 85 1071 5112
Fragments arranged by size:
5112 1071 85
BsmI GAATG_Cn'
Cuts at: 3779 3872 3779
Size: 93 6175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2290 3020 3587 4329 4414 5485 1391
Size: 399 500 730 567 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 730 567 500 399 85
BspEI T'CCGG_A
Cuts at: 5931 5931
Size: 6268
BspHI T'CATG_A
Cuts at: 2503 4157 5887 2503
Size: 1654 1730 2884
Fragments arranged by size:
2884 1730 1654
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3032 3299 3623 844
Size: 2188 267 324 3489
Fragments arranged by size:
3489 2188 324 267
BsrBI CCG'CTC
Cuts at: 1394 1823 4155 1394
Size: 429 2332 3507
Fragments arranged by size:
3507 2332 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2574 3111 3264 4763 4937 65
Size: 1183 1326 537 153 1499 174 1396
Fragments arranged by size:
1499 1396 1326 1183 537 174 153
BsrFI r'CCGG_y
Cuts at: 1700 1810 3272 4912 5099 1700
Size: 110 1462 1640 187 2869
Fragments arranged by size:
2869 1640 1462 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6268
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6268
BssSI C'ACGA_G
Cuts at: 4328 5340 4328
Size: 1012 5256
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2641 3024 794
Size: 1847 383 4038
Fragments arranged by size:
4038 1847 383
BstBI TT'CG_AA
Cuts at: 5072 5072
Size: 6268
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6268
BstXI CCAn_nnnn'nTGG
Cuts at: 2737 3128 2737
Size: 391 5877
BstYI r'GATC_y
Cuts at: 1972 3072 3426 3598 3965 4350 4367 5819 5905
Size: 1100 354 172 367 385 17 1452 86
Cuts at: 5905 5917 6263 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1100 385 367 354 346 172
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 3382 513
Size: 552 939 24 1354 3399
Fragments arranged by size:
3399 1354 939 552 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5529
Fragments arranged by size:
5529 683 56
BtsI GCAGTG_nn'
Cuts at: 3283 3784 4578 4598 3283
Size: 501 794 20 4953
Fragments arranged by size:
4953 794 501 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6233
DraI TTT'AAA
Cuts at: 3572 3572
Size: 6268
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2321 5275 809
Size: 507 1005 2954 1802
Fragments arranged by size:
2954 1802 1005 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2739 3469
Size: 54 1425 50 275 18 300 609 730
Cuts at: 3469 3715 4604 6233 8
Size: 246 889 1629 43
Fragments arranged by size:
1629 1425 889 730 609 300 275 246
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3715 1537
Size: 275 1903 4090
Fragments arranged by size:
4090 1903 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2029 2103 2223 2383 2475 2577 2811 4198
Size: 676 74 120 160 92 102 234 1387
Cuts at: 4198 1353
Size: 3423
Fragments arranged by size:
3423 1387 676 234 160 120 102 92
74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4855 5229 5375 387
Size: 1064 138 3266 374 146 1280
Fragments arranged by size:
3266 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2229 2985 3476 3585 4331 4928 5715
Size: 393 597 756 491 109 746 597 787
Cuts at: 5715 5983 1239
Size: 268 1524
Fragments arranged by size:
1524 787 756 746 597 597 491 393
268 109
EcoNI CCTnn'n_nnAGG
Cuts at: 3080 3080
Size: 6268
EcoO109I rG'GnC_Cy
Cuts at: 2375 2622 3178 3304 3543 2375
Size: 247 556 126 239 5100
Fragments arranged by size:
5100 556 247 239 126
EcoRI G'AATT_C
Cuts at: 3577 3955 3577
Size: 378 5890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2183 2351 2183
Size: 168 6100
FspI TGC'GCA
Cuts at: 4774 4774
Size: 6268
HaeII r_GCGC'y
Cuts at: 1678 3046 5415 1678
Size: 1368 2369 2531
Fragments arranged by size:
2531 2369 1368
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2110 2142 2447 2479 3064
Size: 32 135 32 538 32 305 32 585
Cuts at: 3064 3096 3183 3215 4909 4941 4983 5015 1373
Size: 32 87 32 1694 32 42 32 2626
Fragments arranged by size:
2626 1694 585 538 305 135 87 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2884 3612 3858 669
Size: 988 281 946 728 246 3079
Fragments arranged by size:
3079 988 946 728 281 246
HindIII A'AGCT_T
Cuts at: 748 2328 2918 748
Size: 1580 590 4098
Fragments arranged by size:
4098 1580 590
HpaI GTT'AAC
Cuts at: 3858 3858
Size: 6268
KpnI G_GTAC'C
Cuts at: 3593 3593
Size: 6268
MfeI C'AATT_G
Cuts at: 2835 3867 2835
Size: 1032 5236
MluI A'CGCG_T
Cuts at: 6144 6144
Size: 6268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2732 3334 5382 5566 1305
Size: 1427 602 2048 184 2007
Fragments arranged by size:
2048 2007 1427 602 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2132 2741 3471 10
Size: 54 1425 343 300 609 730 2807
Fragments arranged by size:
2807 1425 730 609 343 300 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3101 3126 4226 4585 4744 518
Size: 648 1935 25 1100 359 159 2042
Fragments arranged by size:
2042 1935 1100 648 359 159 25
MspA1I CmG'CkG
Cuts at: 1606 3316 3454 3684 4361 5509 5754 1606
Size: 1710 138 230 677 1148 245 2120
Fragments arranged by size:
2120 1710 1148 677 245 230 138
NcoI C'CATG_G
Cuts at: 513 1065 2004 3382 513
Size: 552 939 1378 3399
Fragments arranged by size:
3399 1378 939 552
NdeI CA'TA_TG
Cuts at: 387 3535 387
Size: 3148 3120
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6268
NotI GC'GGCC_GC
Cuts at: 3715 3715
Size: 6268
NruI TCG'CGA
Cuts at: 2017 2017
Size: 6268
NsiI A_TGCA'T
Cuts at: 3163 3163
Size: 6268
NspI r_CATG'y
Cuts at: 1132 1229 3626 5171 1132
Size: 97 2397 1545 2229
Fragments arranged by size:
2397 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2562 1348
Size: 1214 5054
PciI A'CATG_T
Cuts at: 5167 5167
Size: 6268
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3097 3429 1108
Size: 1989 332 3947
Fragments arranged by size:
3947 1989 332
PmeI GTTT'AAAC
Cuts at: 3572 3572
Size: 6268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4337 4369 5850 5882 4337
Size: 32 1481 32 4723
Fragments arranged by size:
4723 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2375 2622 3178 3304 3543 2375
Size: 247 556 126 239 5100
Fragments arranged by size:
5100 556 247 239 126
PshAI GACnn'nnGTC
Cuts at: 1613 3362 1613
Size: 1749 4519
PsiI TTA'TAA
Cuts at: 3838 3838
Size: 6268
PstI C_TGCA'G
Cuts at: 830 3620 830
Size: 2790 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6268
SalI G'TCGA_C
Cuts at: 1936 3610 1936
Size: 1674 4594
SapI GCTCTTCn'nnn_
Cuts at: 2223 2223
Size: 6268
SbfI CC_TGCA'GG
Cuts at: 3620 3620
Size: 6268
ScaI AGT'ACT
Cuts at: 4516 6228 4516
Size: 1712 4556
SexAI A'CCwGG_T
Cuts at: 2495 2495
Size: 6268
SfcI C'TryA_G
Cuts at: 826 1045 3616 4751 5432 5623 826
Size: 219 2571 1135 681 191 1471
Fragments arranged by size:
2571 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6268
SmaI CCC'GGG
Cuts at: 1872 3595 1872
Size: 1723 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3063 4372 5273 5535 820
Size: 197 926 1120 1309 901 262 1553
Fragments arranged by size:
1553 1309 1120 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4059 493
Size: 3566 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6268
SphI G_CATG'C
Cuts at: 1132 3626 1132
Size: 2494 3774
SspI AAT'ATT
Cuts at: 5 52 2194 4192 5
Size: 47 2142 1998 2081
Fragments arranged by size:
2142 2081 1998 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6268
StyI C'CwwG_G
Cuts at: 513 1065 2004 2343 2865 3382 3707 513
Size: 552 939 339 522 517 325 3074
Fragments arranged by size:
3074 939 552 522 517 339 325
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3089 3453 4295 4312 4465 4650 5095
Size: 1255 1509 364 842 17 153 185 445
Cuts at: 5095 325
Size: 1498
Fragments arranged by size:
1509 1498 1255 842 445 364 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4514 6226 96
Size: 274 80 33 51 1443 2537 1712 138
Fragments arranged by size:
2537 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6228 6260 6228
Size: 32 6236
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6268
XbaI T'CTAG_A
Cuts at: 3604 3604
Size: 6268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2863 2863
Size: 6268
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6268
XmaI C'CCGG_G
Cuts at: 1870 3593 1870
Size: 1723 4545
XmnI GAAnn'nnTTC
Cuts at: 4397 4397
Size: 6268
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4076 276
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI