Restriction map for
pFN21AB5300
(Circular) (Six-base) MAPSORT of: pFN21AB5300.seq Check: 781 from: 1 to: 5956
pFN21AB5300 5956 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2073 3299 1937
Size: 136 1226 4594
Fragments arranged by size:
4594 1226 136
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4019 5403 5671 1632
Size: 2387 1384 268 1917
Fragments arranged by size:
2387 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2738 820
Size: 197 1721 4038
Fragments arranged by size:
4038 1721 197
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 2812 4685 2812
Size: 1873 4083
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 2270
Size: 32 5924
AlwNI CAG_nnn'CTG
Cuts at: 2249 2342 3699 5271 2249
Size: 93 1357 1572 2934
Fragments arranged by size:
2934 1572 1357 93
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 2147 3265 3491 3643 5752 1493
Size: 654 1118 226 152 2109 1697
Fragments arranged by size:
2109 1697 1118 654 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 2991 3051 3281 1870
Size: 73 1048 60 230 4545
Fragments arranged by size:
4545 1048 230 73 60
AvrII C'CTAG_G
Cuts at: 2194 2194
Size: 5956
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3194 3277 4732 618
Size: 325 217 627 1407 83 1455 1842
Fragments arranged by size:
1842 1455 1407 627 325 217 83
BanII G_rGCy'C
Cuts at: 1391 2521 2615 3210 3275 1391
Size: 1130 94 595 65 4072
Fragments arranged by size:
4072 1130 595 94 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4985
BbvCI CC'TCA_GC
Cuts at: 2979 2979
Size: 5956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4147 4181 1903
Size: 34 2210 34 3678
Fragments arranged by size:
3678 2210 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2196 2912 4567
Size: 107 122 71 631 605 524 716 1655
Cuts at: 4567 136
Size: 1525
Fragments arranged by size:
1655 1525 716 631 605 524 122 107
71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 2997 3373 2997
Size: 376 5580
Bme1580I G_kGCm'C
Cuts at: 1790 2217 4017 5173 1790
Size: 427 1800 1156 2573
Fragments arranged by size:
2573 1800 1156 427
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3195 3687 4645
Size: 416 53 83 399 24 1778 492 958
Cuts at: 4645 442
Size: 1753
Fragments arranged by size:
1778 1753 958 492 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 4616 1239
Size: 1142 2235 2579
Fragments arranged by size:
2579 2235 1142
Bpu10I CC'TnA_GC
Cuts at: 2979 2979
Size: 5956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 4946 5244 4081
Size: 865 298 4793
Fragments arranged by size:
4793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4619 882
Size: 617 3120 2219
Fragments arranged by size:
3120 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3763 4145 4780 275
Size: 53 83 186 936 2230 382 635 1451
Fragments arranged by size:
2230 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2216 2246 2637 2667 1178
Size: 30 165 30 813 30 391 30 4467
Fragments arranged by size:
4467 813 391 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5956
BseYI C'CCAG_C
Cuts at: 2269 2335 2342 5159 5655 2269
Size: 66 7 2817 496 2570
Fragments arranged by size:
2817 2570 496 66 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5383
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2748 3185 3210 4017 4102 5173 2748
Size: 437 25 807 85 1071 3531
Fragments arranged by size:
3531 1071 807 437 85 25
BsmI GAATG_Cn'
Cuts at: 2893 3467 3560 2893
Size: 574 93 5289
Fragments arranged by size:
5289 574 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2521 2615 2748 3185 3210 3275
Size: 399 427 304 94 133 437 25 65
Cuts at: 3275 4017 4102 5173 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 437 427 399 304 133
94 85 65 25
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2935 3311 844
Size: 2091 376 3489
Fragments arranged by size:
3489 2091 376
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4451 4625 65
Size: 1183 3203 174 1396
Fragments arranged by size:
3203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2199 4600 4787 1700
Size: 110 389 2401 187 2869
Fragments arranged by size:
2869 2401 389 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 4016 5028 4016
Size: 1012 4944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstYI r'GATC_y
Cuts at: 1972 2664 3286 3653 4038 4055 5507 5593 5605
Size: 692 622 367 385 17 1452 86 12
Cuts at: 5605 5951 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 692 622 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2529 513
Size: 552 1464 3940
Fragments arranged by size:
3940 1464 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 2505 2865 3129 3472 4266 4286 2505
Size: 360 264 343 794 20 4175
Fragments arranged by size:
4175 794 360 343 264 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2631 2899 3403
Size: 54 1425 50 275 18 801 268 504
Cuts at: 3403 4292 5921 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 801 504 275 268 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2778 3886 1353
Size: 1425 1108 3423
Fragments arranged by size:
3423 1425 1108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2658 4543 4917 5063 387
Size: 1064 138 1069 1885 374 146 1280
Fragments arranged by size:
1885 1280 1069 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3208 3208
Size: 5956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 4019 4616 5403 5671 1239
Size: 393 749 1638 597 787 268 1524
Fragments arranged by size:
1638 1524 787 749 597 393 268
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2863 2251
Size: 612 5344
FseI GG_CCGG'CC
Cuts at: 2203 2203
Size: 5956
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 5103 1678
Size: 3425 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2216 2248 2798 2830 4597
Size: 32 135 32 644 32 550 32 1767
Cuts at: 4597 4629 4671 4703 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1767 644 550 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2074 2857 3300 3546 669
Size: 988 281 136 783 443 246 3079
Fragments arranged by size:
3079 988 783 443 281 246 136
HindIII A'AGCT_T
Cuts at: 748 2448 3042 748
Size: 1700 594 3662
Fragments arranged by size:
3662 1700 594
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2633 2901 10
Size: 54 1425 343 801 268 3065
Fragments arranged by size:
3065 1425 801 343 268 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3914 4273 4432 518
Size: 648 2748 359 159 2042
Fragments arranged by size:
2748 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2339 3372 4049 5197 5442 1606
Size: 733 1033 677 1148 245 2120
Fragments arranged by size:
2120 1148 1033 733 677 245
NaeI GCC'GGC
Cuts at: 2201 2201
Size: 5956
NcoI C'CATG_G
Cuts at: 513 1065 2529 513
Size: 552 1464 3940
Fragments arranged by size:
3940 1464 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NgoMIV G'CCGG_C
Cuts at: 2199 2199
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 3314 4859 1132
Size: 97 2085 1545 2229
Fragments arranged by size:
2229 2085 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2342 1108
Size: 1234 4722
PfoI T'CCnGG_A
Cuts at: 3210 3210
Size: 5956
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3049 3081 4025 4057 5538 5570 3049
Size: 32 944 32 1481 32 3435
Fragments arranged by size:
3435 1481 944 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2952 1613
Size: 1339 4617
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PstI C_TGCA'G
Cuts at: 830 3308 830
Size: 2478 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
PvuII CAG'CTG
Cuts at: 2339 2339
Size: 5956
SacI G_AGCT'C
Cuts at: 3210 3210
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 2072 3298 1936
Size: 136 1226 4594
Fragments arranged by size:
4594 1226 136
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 2231 4204 5916 2231
Size: 1973 1712 2271
Fragments arranged by size:
2271 1973 1712
SfcI C'TryA_G
Cuts at: 826 1045 2250 3304 4439 5120 5311 826
Size: 219 1205 1054 1135 681 191 1471
Fragments arranged by size:
1471 1205 1135 1054 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3053 3283 1872
Size: 1181 230 4545
Fragments arranged by size:
4545 1181 230
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2738 4060 4961 5223 820
Size: 197 926 795 1322 901 262 1553
Fragments arranged by size:
1553 1322 926 901 795 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3253 3880 5
Size: 47 3201 627 2081
Fragments arranged by size:
3201 2081 627 47
StuI AGG'CCT
Cuts at: 1517 2603 1517
Size: 1086 4870
StyI C'CwwG_G
Cuts at: 513 1065 2194 2529 2604 3395 513
Size: 552 1129 335 75 791 3074
Fragments arranged by size:
3074 1129 791 552 335 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3983 4000 4153 4338 4783 325
Size: 1255 2403 17 153 185 445 1498
Fragments arranged by size:
2403 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2229 4202 5914
Size: 274 80 33 51 1443 252 1973 1712
Cuts at: 5914 96
Size: 138
Fragments arranged by size:
1973 1712 1443 274 252 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5916 5948 5916
Size: 32 5924
Tth111I GACn'n_nGTC
Cuts at: 1316 2693 2861 1316
Size: 1377 168 4411
Fragments arranged by size:
4411 1377 168
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3067 3182 3067
Size: 115 5841
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3051 3281 1870
Size: 1181 230 4545
Fragments arranged by size:
4545 1181 230
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI