Restriction map for
pFN21AB5298
(Circular) (Six-base) MAPSORT of: pFN21AB5298.seq Check: 4971 from: 1 to: 6607
pFN21AB5298 6607 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2671 3365 2671
Size: 694 5913
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4417 278
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2469 2592 3057 3950 1937
Size: 532 123 465 893 4594
Fragments arranged by size:
4594 893 532 465 123
Acc65I G'GTAC_C
Cuts at: 3928 3928
Size: 6607
AclI AA'CG_TT
Cuts at: 1563 4734 5107 1563
Size: 3171 373 3063
Fragments arranged by size:
3171 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2212 2378 4670 6054 6322 1632
Size: 580 166 2292 1384 268 1917
Fragments arranged by size:
2292 1917 1384 580 268 166
AfeI AGC'GCT
Cuts at: 3257 3257
Size: 6607
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6410
AflIII A'CryG_T
Cuts at: 1203 5506 6483 1203
Size: 4303 977 1327
Fragments arranged by size:
4303 1327 977
AgeI A'CCGG_T
Cuts at: 5438 5438
Size: 6607
AhdI GACnn_n'nnGTC
Cuts at: 5336 5336
Size: 6607
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6607
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3015 3056 3641 1184
Size: 1831 41 585 4150
Fragments arranged by size:
4150 1831 585 41
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2006 2038 2006
Size: 32 6575
AlwNI CAG_nnn'CTG
Cuts at: 2110 2773 3157 4350 5922 2110
Size: 663 384 1193 1572 2795
Fragments arranged by size:
2795 1572 1193 663 384
ApaI G_GGCC'C
Cuts at: 2656 3735 2656
Size: 1079 5528
ApaLI G'TGCA_C
Cuts at: 3536 3692 4664 5820 3536
Size: 156 972 1156 4323
Fragments arranged by size:
4323 1156 972 156
ApoI r'AATT_y
Cuts at: 1493 3916 4142 4294 6403 1493
Size: 2423 226 152 2109 1697
Fragments arranged by size:
2423 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3451 3451
Size: 6607
AseI AT'TA_AT
Cuts at: 160 1032 5161 160
Size: 872 4129 1606
Fragments arranged by size:
4129 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
AvaI C'yCGr_G
Cuts at: 1870 1943 3932 1870
Size: 73 1989 4545
Fragments arranged by size:
4545 1989 73
BamHI G'GATC_C
Cuts at: 3937 4304 3937
Size: 367 6240
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3928 5383 618
Size: 325 217 627 2141 1455 1842
Fragments arranged by size:
2141 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2349 2656 2715 2870 3735 3926 1391
Size: 958 307 59 155 865 191 4072
Fragments arranged by size:
4072 958 865 307 191 155 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5636
BbvCI CC'TCA_GC
Cuts at: 2603 2757 2945 2603
Size: 154 188 6265
Fragments arranged by size:
6265 188 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2167 2201 4798 4832 1903
Size: 34 230 34 2597 34 3678
Fragments arranged by size:
3678 2597 230 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3515 4499 5709 3515
Size: 984 1210 4413
Fragments arranged by size:
4413 1210 984
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6607
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3008 3448 3684
Size: 107 122 71 631 605 1336 440 236
Cuts at: 3684 5218 136
Size: 1534 1525
Fragments arranged by size:
1534 1525 1336 631 605 440 236 122
107 71
BglII A'GATC_T
Cuts at: 2697 6602 2697
Size: 3905 2702
BlpI GC'TnA_GC
Cuts at: 2348 4024 2348
Size: 1676 4931
Bme1580I G_kGCm'C
Cuts at: 1790 2656 3540 3606 3696 3735 4668 5824 1790
Size: 866 884 66 90 39 933 1156 2573
Fragments arranged by size:
2573 1156 933 884 866 90 66 39
BmgBI CAC'GTC
Cuts at: 1318 3535 3564 1318
Size: 2217 29 4361
Fragments arranged by size:
4361 2217 29
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2987 3886 4338
Size: 416 53 83 399 24 1570 899 452
Cuts at: 4338 5296 442
Size: 958 1753
Fragments arranged by size:
1753 1570 958 899 452 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2639 2696 3133 3356 3880 5267 1239
Size: 1124 276 57 437 223 524 1387 2579
Fragments arranged by size:
2579 1387 1124 524 437 276 223 57
Bpu10I CC'TnA_GC
Cuts at: 2354 2603 2757 2945 2354
Size: 249 154 188 6016
Fragments arranged by size:
6016 249 188 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2515 2747 3284 3821 4732 5597 5895 2515
Size: 232 537 537 911 865 298 3227
Fragments arranged by size:
3227 911 865 537 537 298 232
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2627 2629 5270 882
Size: 617 1128 2 2641 2219
Fragments arranged by size:
2641 2219 1128 617 2
BsaAI yAC'GTr
Cuts at: 493 1204 3289 3691 4398 493
Size: 711 2085 402 707 2702
Fragments arranged by size:
2702 2085 711 707 402
BsaBI GATnn'nnATC
Cuts at: 3350 6601 3350
Size: 3251 3356
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4414 4796 5431 275
Size: 53 83 186 936 2881 382 635 1451
Fragments arranged by size:
2881 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2835 5040 5438 5712 5859 6270 2835
Size: 2205 398 274 147 411 3172
Fragments arranged by size:
3172 2205 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2006 2036 2870 2900 1178
Size: 30 165 30 603 30 834 30 4885
Fragments arranged by size:
4885 834 603 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2591 3467 3896 1186
Size: 841 564 876 429 3897
Fragments arranged by size:
3897 876 841 564 429
BseYI C'CCAG_C
Cuts at: 2028 2243 2792 2855 3599 3720 3735 5810 6306
Size: 215 549 63 744 121 15 2075 496
Cuts at: 6306 2028
Size: 2329
Fragments arranged by size:
2329 2075 744 549 496 215 121 63
15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2031 2690 2705 3544 1163
Size: 573 295 659 15 839 4226
Fragments arranged by size:
4226 839 659 573 295 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2888 3364 4057 4818 5846 1540
Size: 275 186 887 476 693 761 1028 2301
Fragments arranged by size:
2301 1028 887 761 693 476 275 186
BsiHKAI G_wGCw'C
Cuts at: 2349 3045 3171 3540 3696 3777 4668 4753 5824
Size: 696 126 369 156 81 891 85 1071
Cuts at: 5824 2349
Size: 3132
Fragments arranged by size:
3132 1071 891 696 369 156 126 85
81
BsmI GAATG_Cn'
Cuts at: 2919 3250 4118 4211 2919
Size: 331 868 93 5315
Fragments arranged by size:
5315 868 331 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2349 2656 2715 2870 3045 3171 3540
Size: 399 559 307 59 155 175 126 369
Cuts at: 3540 3606 3696 3735 3777 3926 4668 4753 5824
Size: 66 90 39 42 149 742 85 1071
Cuts at: 5824 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 559 399 369 307 175
155 149 126 90 85 66 59 42
39
BspEI T'CCGG_A
Cuts at: 6270 6270
Size: 6607
BspHI T'CATG_A
Cuts at: 4496 6226 4496
Size: 1730 4877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2671 3189 3365 3962 844
Size: 1827 518 176 597 3489
Fragments arranged by size:
3489 1827 597 518 176
BsrBI CCG'CTC
Cuts at: 1394 1823 3660 3789 4494 1394
Size: 429 1837 129 705 3507
Fragments arranged by size:
3507 1837 705 429 129
BsrDI GCAATG_nn'
Cuts at: 65 1248 2365 3618 5102 5276 65
Size: 1183 1117 1253 1484 174 1396
Fragments arranged by size:
1484 1396 1253 1183 1117 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2472 5251 5438 1700
Size: 110 662 2779 187 2869
Fragments arranged by size:
2869 2779 662 187 110
BsrGI T'GTAC_A
Cuts at: 96 2362 96
Size: 2266 4341
BssHII G'CGCG_C
Cuts at: 1925 3451 1925
Size: 1526 5081
BssSI C'ACGA_G
Cuts at: 2708 4667 5679 2708
Size: 1959 1012 3636
Fragments arranged by size:
3636 1959 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2773 794
Size: 1979 4628
BstBI TT'CG_AA
Cuts at: 5411 5411
Size: 6607
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6607
BstYI r'GATC_y
Cuts at: 1972 2397 2697 3147 3639 3937 4304 4689 4706
Size: 425 300 450 492 298 367 385 17
Cuts at: 4706 6158 6244 6256 6602 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 492 450 425 385 367 346
300 298 86 17 12
BstZ17I GTA'TAC
Cuts at: 3058 3058
Size: 6607
BtgI C'CryG_G
Cuts at: 513 1065 2004 2284 3125 3670 3811 513
Size: 552 939 280 841 545 141 3309
Fragments arranged by size:
3309 939 841 552 545 280 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2180 3019 487
Size: 683 56 954 839 4075
Fragments arranged by size:
4075 954 839 683 56
BtsI GCAGTG_nn'
Cuts at: 2065 2997 3394 4123 4917 4937 2065
Size: 932 397 729 794 20 3735
Fragments arranged by size:
3735 932 794 729 397 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6607
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6572
DraI TTT'AAA
Cuts at: 3911 3911
Size: 6607
DraIII CAC_nnn'GTG
Cuts at: 2071 2290 3544 2071
Size: 219 1254 5134
Fragments arranged by size:
5134 1254 219
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3080 5614 809
Size: 507 1764 2534 1802
Fragments arranged by size:
2534 1802 1764 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2814 3361 3655
Size: 54 1425 50 275 18 984 547 294
Cuts at: 3655 4054 4943 6572 8
Size: 399 889 1629 43
Fragments arranged by size:
1629 1425 984 889 547 399 294 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3361 4054 1537
Size: 275 1549 693 4090
Fragments arranged by size:
4090 1549 693 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2156 2265 2445 4537 1353
Size: 803 109 180 2092 3423
Fragments arranged by size:
3423 2092 803 180 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5194 5568 5714 387
Size: 1064 138 3605 374 146 1280
Fragments arranged by size:
3605 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2347 2347
Size: 6607
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2363 2378 2639 2696 3133 3356
Size: 393 580 151 15 261 57 437 223
Cuts at: 3356 3880 4670 5267 6054 6322 1239
Size: 524 790 597 787 268 1524
Fragments arranged by size:
1524 790 787 597 580 524 437 393
268 261 223 151 57 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2678 3161 2678
Size: 483 6124
EcoO109I rG'GnC_Cy
Cuts at: 2275 2652 2653 2761 3731 3732 3816 2275
Size: 377 1 108 970 1 84 5066
Fragments arranged by size:
5066 970 377 108 84 1 1
EcoRI G'AATT_C
Cuts at: 3916 4294 3916
Size: 378 6229
FspI TGC'GCA
Cuts at: 5113 5113
Size: 6607
HaeII r_GCGC'y
Cuts at: 1678 2823 3259 5754 1678
Size: 1145 436 2495 2531
Fragments arranged by size:
2531 2495 1145 436
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2684 2716 2870 2902 3836
Size: 32 135 32 1112 32 154 32 934
Cuts at: 3836 3868 5248 5280 5322 5354 1373
Size: 32 1380 32 42 32 2626
Fragments arranged by size:
2626 1380 1112 934 154 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3951 4197 669
Size: 988 281 2013 246 3079
Fragments arranged by size:
3079 2013 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6607
HpaI GTT'AAC
Cuts at: 4197 4197
Size: 6607
KpnI G_GTAC'C
Cuts at: 3932 3932
Size: 6607
MfeI C'AATT_G
Cuts at: 4206 4206
Size: 6607
MluI A'CGCG_T
Cuts at: 6483 6483
Size: 6607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2868 3048 5721 5905 1305
Size: 1563 180 2673 184 2007
Fragments arranged by size:
2673 2007 1563 184 180
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2816 10
Size: 54 1425 343 984 3801
Fragments arranged by size:
3801 1425 984 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3038 3623 4565 4924 5083 518
Size: 648 1872 585 942 359 159 2042
Fragments arranged by size:
2042 1872 942 648 585 359 159
MspA1I CmG'CkG
Cuts at: 1606 2142 2746 2770 3138 3558 3672 3739 3813
Size: 536 604 24 368 420 114 67 74
Cuts at: 3813 4023 4700 5848 6093 1606
Size: 210 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 604 536 420 368 245
210 114 74 67 24
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5116
Fragments arranged by size:
5116 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6607
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6607
NotI GC'GGCC_GC
Cuts at: 3361 4054 3361
Size: 693 5914
NspI r_CATG'y
Cuts at: 1132 1229 3022 3965 5510 1132
Size: 97 1793 943 1545 2229
Fragments arranged by size:
2229 1793 1545 943 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6607
PciI A'CATG_T
Cuts at: 5506 5506
Size: 6607
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3279 1108
Size: 2171 4436
PfoI T'CCnGG_A
Cuts at: 2133 2133
Size: 6607
PmeI GTTT'AAAC
Cuts at: 3911 3911
Size: 6607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2006 2038 4676 4708 6189 6221 2006
Size: 32 2638 32 1481 32 2392
Fragments arranged by size:
2638 2392 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2275 2761 3816 2275
Size: 486 1055 5066
Fragments arranged by size:
5066 1055 486
PshAI GACnn'nnGTC
Cuts at: 1613 3574 1613
Size: 1961 4646
PsiI TTA'TAA
Cuts at: 4177 4177
Size: 6607
PspOMI G'GGCC_C
Cuts at: 2652 3731 2652
Size: 1079 5528
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2066 2098 2066
Size: 32 6575
PstI C_TGCA'G
Cuts at: 830 2769 2865 3162 3959 830
Size: 1939 96 297 797 3478
Fragments arranged by size:
3478 1939 797 297 96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6607
PvuII CAG'CTG
Cuts at: 3558 3739 3558
Size: 181 6426
SacI G_AGCT'C
Cuts at: 2349 2349
Size: 6607
SacII CC_GC'GG
Cuts at: 3673 3814 3673
Size: 141 6466
SalI G'TCGA_C
Cuts at: 1936 3949 1936
Size: 2013 4594
SanDI GG'GwC_CC
Cuts at: 3816 3816
Size: 6607
SbfI CC_TGCA'GG
Cuts at: 3959 3959
Size: 6607
ScaI AGT'ACT
Cuts at: 4855 6567 4855
Size: 1712 4895
SexAI A'CCwGG_T
Cuts at: 3540 3540
Size: 6607
SfcI C'TryA_G
Cuts at: 826 1045 2765 2849 2861 3158 3553 3955 5090
Size: 219 1720 84 12 297 395 402 1135
Cuts at: 5090 5771 5962 826
Size: 681 191 1471
Fragments arranged by size:
1720 1471 1135 681 402 395 297 219
191 84 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6607
SmaI CCC'GGG
Cuts at: 1872 3934 1872
Size: 2062 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2494 2726 3299 3800 4711 5612
Size: 197 926 551 232 573 501 911 901
Cuts at: 5612 5874 820
Size: 262 1553
Fragments arranged by size:
1553 926 911 901 573 551 501 262
232 197
SnaBI TAC'GTA
Cuts at: 493 4398 493
Size: 3905 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6607
SphI G_CATG'C
Cuts at: 1132 3965 1132
Size: 2833 3774
SspI AAT'ATT
Cuts at: 5 52 4531 5
Size: 47 4479 2081
Fragments arranged by size:
4479 2081 47
StuI AGG'CCT
Cuts at: 1517 3759 1517
Size: 2242 4365
StyI C'CwwG_G
Cuts at: 513 1065 2004 3085 4046 513
Size: 552 939 1081 961 3074
Fragments arranged by size:
3074 1081 961 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2042 2076 2431 2651 4634 4651 4804
Size: 1255 462 34 355 220 1983 17 153
Cuts at: 4804 4989 5434 325
Size: 185 445 1498
Fragments arranged by size:
1983 1498 1255 462 445 355 220 185
153 34 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2362 4853 6565
Size: 274 80 33 51 1443 385 2491 1712
Cuts at: 6565 96
Size: 138
Fragments arranged by size:
2491 1712 1443 385 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2276 2308 2882 2914 6567 6599 2276
Size: 32 574 32 3653 32 2284
Fragments arranged by size:
3653 2284 574 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6607
XbaI T'CTAG_A
Cuts at: 3943 3943
Size: 6607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3757 3757
Size: 6607
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6607
XmaI C'CCGG_G
Cuts at: 1870 3932 1870
Size: 2062 4545
XmnI GAAnn'nnTTC
Cuts at: 2993 4736 2993
Size: 1743 4864
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4415 276
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI
Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI RsrII SapI
SfiI SfoI SrfI SwaI