Restriction map for
pFN21AB5291
(Circular) (Six-base) MAPSORT of: pFN21AB5291.seq Check: 6209 from: 1 to: 5212
pFN21AB5291 5212 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3022 278
Size: 53 83 186 936 1486 2468
Fragments arranged by size:
2468 1486 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2283 2555 1937
Size: 346 272 4594
Fragments arranged by size:
4594 346 272
Acc65I G'GTAC_C
Cuts at: 2533 2533
Size: 5212
AclI AA'CG_TT
Cuts at: 1563 3339 3712 1563
Size: 1776 373 3063
Fragments arranged by size:
3063 1776 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2516 3275 4659 4927 1632
Size: 884 759 1384 268 1917
Fragments arranged by size:
1917 1384 884 759 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5015
AflIII A'CryG_T
Cuts at: 1203 4111 5088 1203
Size: 2908 977 1327
Fragments arranged by size:
2908 1327 977
AgeI A'CCGG_T
Cuts at: 4043 4043
Size: 5212
AhdI GACnn_n'nnGTC
Cuts at: 3941 3941
Size: 5212
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5212
AlwNI CAG_nnn'CTG
Cuts at: 2019 2955 4527 2019
Size: 936 1572 2704
Fragments arranged by size:
2704 1572 936
ApaI G_GGCC'C
Cuts at: 2211 2211
Size: 5212
ApaLI G'TGCA_C
Cuts at: 3269 4425 3269
Size: 1156 4056
ApoI r'AATT_y
Cuts at: 1493 2034 2521 2747 2899 5008 1493
Size: 541 487 226 152 2109 1697
Fragments arranged by size:
2109 1697 541 487 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3766 160
Size: 872 2734 1606
Fragments arranged by size:
2734 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5212
AvaI C'yCGr_G
Cuts at: 1870 1943 2180 2537 1870
Size: 73 237 357 4545
Fragments arranged by size:
4545 357 237 73
BamHI G'GATC_C
Cuts at: 2542 2909 2542
Size: 367 4845
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2022 2533 3988 618
Size: 325 217 627 221 14 511 1455 1842
Fragments arranged by size:
1842 1455 627 511 325 221 217 14
BanII G_rGCy'C
Cuts at: 1391 2211 2531 1391
Size: 820 320 4072
Fragments arranged by size:
4072 820 320
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3403 3437 1903
Size: 34 1466 34 3678
Fragments arranged by size:
3678 1466 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3104 4314 3104
Size: 1210 4002
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5212
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3823 136
Size: 107 122 71 631 605 2151 1525
Fragments arranged by size:
2151 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5207 5207
Size: 5212
BlpI GC'TnA_GC
Cuts at: 2629 2629
Size: 5212
Bme1580I G_kGCm'C
Cuts at: 1790 2027 2211 3273 4429 1790
Size: 237 184 1062 1156 2573
Fragments arranged by size:
2573 1156 1062 237 184
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5212
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2943 3901 442
Size: 416 53 83 399 24 1526 958 1753
Fragments arranged by size:
1753 1526 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5212
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2309 3872 1239
Size: 1070 1563 2579
Fragments arranged by size:
2579 1563 1070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3337 4202 4500 3337
Size: 865 298 4049
Fragments arranged by size:
4049 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3875 882
Size: 617 2376 2219
Fragments arranged by size:
2376 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3003 493
Size: 711 1799 2702
Fragments arranged by size:
2702 1799 711
BsaBI GATnn'nnATC
Cuts at: 5206 5206
Size: 5212
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3019 3401 4036 275
Size: 53 83 186 936 1486 382 635 1451
Fragments arranged by size:
1486 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3645 4043 4317 4464 4875 3645
Size: 398 274 147 411 3982
Fragments arranged by size:
3982 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4987
Fragments arranged by size:
4987 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 1186
Size: 1021 4191
BseYI C'CCAG_C
Cuts at: 2026 4415 4911 2026
Size: 2389 496 2327
Fragments arranged by size:
2389 2327 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2475 1163
Size: 573 739 3900
Fragments arranged by size:
3900 739 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2662 3423 4451 1540
Size: 275 186 661 761 1028 2301
Fragments arranged by size:
2301 1028 761 661 275 186
BsiHKAI G_wGCw'C
Cuts at: 3273 3358 4429 3273
Size: 85 1071 4056
Fragments arranged by size:
4056 1071 85
BsmI GAATG_Cn'
Cuts at: 2723 2816 2723
Size: 93 5119
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2211 2531 3273 3358 4429 1391
Size: 399 237 184 320 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 320 237 184 85
BspEI T'CCGG_A
Cuts at: 4875 4875
Size: 5212
BspHI T'CATG_A
Cuts at: 3101 4831 3101
Size: 1730 3482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2258 2567 844
Size: 1414 309 3489
Fragments arranged by size:
3489 1414 309
BsrBI CCG'CTC
Cuts at: 1394 1823 3099 1394
Size: 429 1276 3507
Fragments arranged by size:
3507 1276 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2082 3707 3881 65
Size: 1183 834 1625 174 1396
Fragments arranged by size:
1625 1396 1183 834 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3856 4043 1700
Size: 110 2046 187 2869
Fragments arranged by size:
2869 2046 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5212
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5212
BssSI C'ACGA_G
Cuts at: 3272 4284 3272
Size: 1012 4200
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5212
BstBI TT'CG_AA
Cuts at: 4016 4016
Size: 5212
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5212
BstXI CCAn_nnnn'nTGG
Cuts at: 2218 2257 2218
Size: 39 5173
BstYI r'GATC_y
Cuts at: 1972 2150 2542 2909 3294 3311 4763 4849 4861
Size: 178 392 367 385 17 1452 86 12
Cuts at: 4861 5207 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 392 385 367 346 178 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2211 513
Size: 552 939 207 3514
Fragments arranged by size:
3514 939 552 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4473
Fragments arranged by size:
4473 683 56
BtsI GCAGTG_nn'
Cuts at: 2728 3522 3542 2728
Size: 794 20 4398
Fragments arranged by size:
4398 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5177
DraI TTT'AAA
Cuts at: 2516 2516
Size: 5212
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4219 809
Size: 507 2903 1802
Fragments arranged by size:
2903 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2659 3548 5177
Size: 54 1425 50 275 18 829 889 1629
Cuts at: 5177 8
Size: 43
Fragments arranged by size:
1629 1425 889 829 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2659 1537
Size: 275 847 4090
Fragments arranged by size:
4090 847 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2077 3142 1353
Size: 724 1065 3423
Fragments arranged by size:
3423 1065 724
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3799 4173 4319 387
Size: 1064 138 2210 374 146 1280
Fragments arranged by size:
2210 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2309 2516 3275 3872 4659 4927 1239
Size: 393 677 207 759 597 787 268 1524
Fragments arranged by size:
1524 787 759 677 597 393 268 207
EcoO109I rG'GnC_Cy
Cuts at: 2207 2207
Size: 5212
EcoRI G'AATT_C
Cuts at: 2521 2899 2521
Size: 378 4834
FspI TGC'GCA
Cuts at: 3718 3718
Size: 5212
HaeII r_GCGC'y
Cuts at: 1678 4359 1678
Size: 2681 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2344 2376 2432 2464 3853
Size: 32 135 32 772 32 56 32 1389
Cuts at: 3853 3885 3927 3959 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1389 772 135 56 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2284 2556 2802 669
Size: 988 281 346 272 246 3079
Fragments arranged by size:
3079 988 346 281 272 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5212
HpaI GTT'AAC
Cuts at: 2802 2802
Size: 5212
KpnI G_GTAC'C
Cuts at: 2537 2537
Size: 5212
MfeI C'AATT_G
Cuts at: 2811 2811
Size: 5212
MluI A'CGCG_T
Cuts at: 5088 5088
Size: 5212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4326 4510 1305
Size: 3021 184 2007
Fragments arranged by size:
3021 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3390
Fragments arranged by size:
3390 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 2216 3170 3529 3688 518
Size: 648 948 102 954 359 159 2042
Fragments arranged by size:
2042 954 948 648 359 159 102
MspA1I CmG'CkG
Cuts at: 1606 2628 3305 4453 4698 1606
Size: 1022 677 1148 245 2120
Fragments arranged by size:
2120 1148 1022 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2211 513
Size: 552 939 207 3514
Fragments arranged by size:
3514 939 552 207
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5212
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5212
NotI GC'GGCC_GC
Cuts at: 2659 2659
Size: 5212
NspI r_CATG'y
Cuts at: 1132 1229 2408 2570 4115 1132
Size: 97 1179 162 1545 2229
Fragments arranged by size:
2229 1545 1179 162 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5212
PciI A'CATG_T
Cuts at: 4111 4111
Size: 5212
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2019 1108
Size: 911 4301
PfoI T'CCnGG_A
Cuts at: 2443 2443
Size: 5212
PmeI GTTT'AAAC
Cuts at: 2516 2516
Size: 5212
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 4794 4826 3281
Size: 32 1481 32 3667
Fragments arranged by size:
3667 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5212
PsiI TTA'TAA
Cuts at: 2474 2782 2474
Size: 308 4904
PspOMI G'GGCC_C
Cuts at: 2207 2207
Size: 5212
PstI C_TGCA'G
Cuts at: 830 2564 830
Size: 1734 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5212
SalI G'TCGA_C
Cuts at: 1936 2282 2554 1936
Size: 346 272 4594
Fragments arranged by size:
4594 346 272
SapI GCTCTTCn'nnn_
Cuts at: 2077 2077
Size: 5212
SbfI CC_TGCA'GG
Cuts at: 2564 2564
Size: 5212
ScaI AGT'ACT
Cuts at: 3460 5172 3460
Size: 1712 3500
SexAI A'CCwGG_T
Cuts at: 2018 2018
Size: 5212
SfcI C'TryA_G
Cuts at: 826 1045 2560 3695 4376 4567 826
Size: 219 1515 1135 681 191 1471
Fragments arranged by size:
1515 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5212
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5212
SmaI CCC'GGG
Cuts at: 1872 2539 1872
Size: 667 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3316 4217 4479 820
Size: 197 926 1373 901 262 1553
Fragments arranged by size:
1553 1373 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3003 493
Size: 2510 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5212
SphI G_CATG'C
Cuts at: 1132 2570 1132
Size: 1438 3774
SspI AAT'ATT
Cuts at: 5 52 3136 5
Size: 47 3084 2081
Fragments arranged by size:
3084 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5212
StyI C'CwwG_G
Cuts at: 513 1065 2004 2211 2651 513
Size: 552 939 207 440 3074
Fragments arranged by size:
3074 939 552 440 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 3239 3256 3409 3594 4039 325
Size: 1255 446 1213 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1213 446 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3458 5170 96
Size: 274 80 33 51 1443 1481 1712 138
Fragments arranged by size:
1712 1481 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5172 5204 5172
Size: 32 5180
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5212
XbaI T'CTAG_A
Cuts at: 2233 2548 2233
Size: 315 4897
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5212
XmaI C'CCGG_G
Cuts at: 1870 2537 1870
Size: 667 4545
XmnI GAAnn'nnTTC
Cuts at: 2434 3341 2434
Size: 907 4305
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3020 276
Size: 53 83 186 936 1486 2468
Fragments arranged by size:
2468 1486 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI XcmI