Restriction map for
pFN21AB5288
(Circular) (Six-base) MAPSORT of: pFN21AB5288.seq Check: 4836 from: 1 to: 6355
pFN21AB5288 6355 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2242 2242
Size: 6355
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4165 278
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3698 1937
Size: 1761 4594
Acc65I G'GTAC_C
Cuts at: 2906 3676 2906
Size: 770 5585
AclI AA'CG_TT
Cuts at: 1563 4482 4855 1563
Size: 2919 373 3063
Fragments arranged by size:
3063 2919 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3137 4418 5802 6070 1632
Size: 1505 1281 1384 268 1917
Fragments arranged by size:
1917 1505 1384 1281 268
AfeI AGC'GCT
Cuts at: 2306 2306
Size: 6355
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6158
AflIII A'CryG_T
Cuts at: 1203 5254 6231 1203
Size: 4051 977 1327
Fragments arranged by size:
4051 1327 977
AgeI A'CCGG_T
Cuts at: 5186 5186
Size: 6355
AhdI GACnn_n'nnGTC
Cuts at: 3162 5084 3162
Size: 1922 4433
AleI CACnn'nnGTG
Cuts at: 1166 2356 1166
Size: 1190 5165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6355
AlwNI CAG_nnn'CTG
Cuts at: 2170 2257 3014 4098 5670 2170
Size: 87 757 1084 1572 2855
Fragments arranged by size:
2855 1572 1084 757 87
ApaI G_GGCC'C
Cuts at: 2581 2581
Size: 6355
ApaLI G'TGCA_C
Cuts at: 4412 5568 4412
Size: 1156 5199
ApoI r'AATT_y
Cuts at: 1493 3664 3890 4042 6151 1493
Size: 2171 226 152 2109 1697
Fragments arranged by size:
2171 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4909 160
Size: 872 3877 1606
Fragments arranged by size:
3877 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
AvaI C'yCGr_G
Cuts at: 1870 1943 3057 3680 1870
Size: 73 1114 623 4545
Fragments arranged by size:
4545 1114 623 73
AvrII C'CTAG_G
Cuts at: 3554 3554
Size: 6355
BamHI G'GATC_C
Cuts at: 3685 4052 3685
Size: 367 5988
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2906 3676 5131 618
Size: 325 217 627 1119 770 1455 1842
Fragments arranged by size:
1842 1455 1119 770 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2581 3562 3674 1391
Size: 1190 981 112 4072
Fragments arranged by size:
4072 1190 981 112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2270 928
Size: 971 371 5013
Fragments arranged by size:
5013 971 371
BbvCI CC'TCA_GC
Cuts at: 2213 2514 3585 2213
Size: 301 1071 4983
Fragments arranged by size:
4983 1071 301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4546 4580 1903
Size: 34 50 34 2525 34 3678
Fragments arranged by size:
3678 2525 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4247 5457 4247
Size: 1210 5145
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6355
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3467 4966 136
Size: 107 122 71 631 605 1795 1499 1525
Fragments arranged by size:
1795 1525 1499 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6350 6350
Size: 6355
BlpI GC'TnA_GC
Cuts at: 2301 2871 3772 2301
Size: 570 901 4884
Fragments arranged by size:
4884 901 570
Bme1580I G_kGCm'C
Cuts at: 1790 2581 2950 4416 5572 1790
Size: 791 369 1466 1156 2573
Fragments arranged by size:
2573 1466 1156 791 369
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6355
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2705 2953 3024
Size: 416 53 83 399 24 1288 248 71
Cuts at: 3024 4086 5044 442
Size: 1062 958 1753
Fragments arranged by size:
1753 1288 1062 958 416 399 248 83
71 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2093 2192 3413 3626 5015 1239
Size: 809 45 99 1221 213 1389 2579
Fragments arranged by size:
2579 1389 1221 809 213 99 45
Bpu10I CC'TnA_GC
Cuts at: 2213 2514 3107 3585 2213
Size: 301 593 478 4983
Fragments arranged by size:
4983 593 478 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4480 5345 5643 4480
Size: 865 298 5192
Fragments arranged by size:
5192 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3502 5018 882
Size: 617 2003 1516 2219
Fragments arranged by size:
2219 2003 1516 617
BsaAI yAC'GTr
Cuts at: 493 1204 4146 493
Size: 711 2942 2702
Fragments arranged by size:
2942 2702 711
BsaBI GATnn'nnATC
Cuts at: 6349 6349
Size: 6355
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4162 4544 5179 275
Size: 53 83 186 936 2629 382 635 1451
Fragments arranged by size:
2629 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2112 2343 4788 5186 5460 5607 6018 2112
Size: 231 2445 398 274 147 411 2449
Fragments arranged by size:
2449 2445 411 398 274 231 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3182 3212 3253 3283 1178
Size: 30 165 30 1779 30 41 30 4250
Fragments arranged by size:
4250 1779 165 41 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2626 2831 1186
Size: 1440 205 4710
Fragments arranged by size:
4710 1440 205
BseYI C'CCAG_C
Cuts at: 2154 2257 2403 2428 2672 2927 3372 3472 5558
Size: 103 146 25 244 255 445 100 2086
Cuts at: 5558 6054 2154
Size: 496 2455
Fragments arranged by size:
2455 2086 496 445 255 244 146 103
100 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2939 1163
Size: 573 1203 4579
Fragments arranged by size:
4579 1203 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2323 3805 4566 5594 1540
Size: 275 186 322 1482 761 1028 2301
Fragments arranged by size:
2301 1482 1028 761 322 275 186
BsiHKAI G_wGCw'C
Cuts at: 2508 4416 4501 5572 2508
Size: 1908 85 1071 3291
Fragments arranged by size:
3291 1908 1071 85
BsmI GAATG_Cn'
Cuts at: 3866 3959 3866
Size: 93 6262
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2508 2581 2950 3562 3674 4416 4501
Size: 399 718 73 369 612 112 742 85
Cuts at: 4501 5572 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 718 612 399 369 112
85 73
BspEI T'CCGG_A
Cuts at: 2112 2343 6018 2112
Size: 231 3675 2449
Fragments arranged by size:
3675 2449 231
BspHI T'CATG_A
Cuts at: 2812 3249 4244 5974 2812
Size: 437 995 1730 3193
Fragments arranged by size:
3193 1730 995 437
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2242 3710 844
Size: 1398 1468 3489
Fragments arranged by size:
3489 1468 1398
BsrBI CCG'CTC
Cuts at: 1394 1823 2634 4242 1394
Size: 429 811 1608 3507
Fragments arranged by size:
3507 1608 811 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2506 3423 4850 5024 65
Size: 1183 1258 917 1427 174 1396
Fragments arranged by size:
1427 1396 1258 1183 917 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2271 2991 4999 5186 1700
Size: 110 461 720 2008 187 2869
Fragments arranged by size:
2869 2008 720 461 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6355
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6355
BssSI C'ACGA_G
Cuts at: 4415 5427 4415
Size: 1012 5343
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6355
BstBI TT'CG_AA
Cuts at: 5159 5159
Size: 6355
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6355
BstXI CCAn_nnnn'nTGG
Cuts at: 2358 2358
Size: 6355
BstYI r'GATC_y
Cuts at: 1972 2109 3685 4052 4437 4454 5906 5992 6004
Size: 137 1576 367 385 17 1452 86 12
Cuts at: 6004 6350 1972
Size: 346 1977
Fragments arranged by size:
1977 1576 1452 385 367 346 137 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2638 2912 3000 2638
Size: 274 88 5993
Fragments arranged by size:
5993 274 88
BtgI C'CryG_G
Cuts at: 513 1065 2317 3596 513
Size: 552 1252 1279 3272
Fragments arranged by size:
3272 1279 1252 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2643 3331 487
Size: 683 56 1417 688 3511
Fragments arranged by size:
3511 1417 688 683 56
BtsI GCAGTG_nn'
Cuts at: 2775 2895 3173 3239 3871 4665 4685 2775
Size: 120 278 66 632 794 20 4445
Fragments arranged by size:
4445 794 632 278 120 66 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6355
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2695 2730 529
Size: 35 2131 35 4154
Fragments arranged by size:
4154 2131 35 35
DraI TTT'AAA
Cuts at: 3659 3659
Size: 6355
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5362 809
Size: 507 4046 1802
Fragments arranged by size:
4046 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2320 3802 4691
Size: 54 1425 50 275 18 490 1482 889
Cuts at: 4691 6320 8
Size: 1629 43
Fragments arranged by size:
1629 1482 1425 889 490 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2320 3802 1537
Size: 275 508 1482 4090
Fragments arranged by size:
4090 1482 508 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 2565 2987 3015 4285 1353
Size: 1160 52 422 28 1270 3423
Fragments arranged by size:
3423 1270 1160 422 52 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4942 5316 5462 387
Size: 1064 138 3353 374 146 1280
Fragments arranged by size:
3353 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2093 2192 3137 3413 3626 4418
Size: 393 416 45 99 945 276 213 792
Cuts at: 4418 5015 5802 6070 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 945 792 787 597 416 393 276
268 213 99 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2641 2641
Size: 6355
EcoO109I rG'GnC_Cy
Cuts at: 2032 2127 2472 2556 2577 2578 3306 2032
Size: 95 345 84 21 1 728 5081
Fragments arranged by size:
5081 728 345 95 84 21 1
EcoRI G'AATT_C
Cuts at: 3664 4042 3664
Size: 378 5977
FspI TGC'GCA
Cuts at: 4861 4861
Size: 6355
HaeII r_GCGC'y
Cuts at: 1678 2308 5502 1678
Size: 630 3194 2531
Fragments arranged by size:
3194 2531 630
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2007 2039 3148 3178 3180
Size: 32 135 32 435 32 1109 30 2
Cuts at: 3180 3210 4996 5028 5070 5102 1373
Size: 30 1786 32 42 32 2626
Fragments arranged by size:
2626 1786 1109 435 135 42 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3046 3699 3945 669
Size: 988 281 1108 653 246 3079
Fragments arranged by size:
3079 1108 988 653 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6355
HpaI GTT'AAC
Cuts at: 3945 3945
Size: 6355
KpnI G_GTAC'C
Cuts at: 2910 3680 2910
Size: 770 5585
MfeI C'AATT_G
Cuts at: 3954 3954
Size: 6355
MluI A'CGCG_T
Cuts at: 6231 6231
Size: 6355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5469 5653 1305
Size: 4164 184 2007
Fragments arranged by size:
4164 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4533
Fragments arranged by size:
4533 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2356 2453 4313 4672 4831 518
Size: 648 1190 97 1860 359 159 2042
Fragments arranged by size:
2042 1860 1190 648 359 159 97
MspA1I CmG'CkG
Cuts at: 1606 2236 2300 2319 2403 2428 3028 3337 3353
Size: 630 64 19 84 25 600 309 16
Cuts at: 3353 3376 3771 4448 5596 5841 1606
Size: 23 395 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 630 600 395 309 245
84 64 25 23 19 16
NaeI GCC'GGC
Cuts at: 2273 2273
Size: 6355
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5803
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6355
NgoMIV G'CCGG_C
Cuts at: 2271 2271
Size: 6355
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6355
NotI GC'GGCC_GC
Cuts at: 3802 3802
Size: 6355
NspI r_CATG'y
Cuts at: 1132 1229 3713 5258 1132
Size: 97 2484 1545 2229
Fragments arranged by size:
2484 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2626 3501 1348
Size: 1278 875 4202
Fragments arranged by size:
4202 1278 875
PciI A'CATG_T
Cuts at: 5254 5254
Size: 6355
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2717 2740 3292 3322 1108
Size: 1609 23 552 30 4141
Fragments arranged by size:
4141 1609 552 30 23
PfoI T'CCnGG_A
Cuts at: 2025 2442 2025
Size: 417 5938
PmeI GTTT'AAAC
Cuts at: 3659 3659
Size: 6355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4424 4456 5937 5969 4424
Size: 32 1481 32 4810
Fragments arranged by size:
4810 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2032 2472 3306 2032
Size: 440 834 5081
Fragments arranged by size:
5081 834 440
PshAI GACnn'nnGTC
Cuts at: 1613 3059 1613
Size: 1446 4909
PsiI TTA'TAA
Cuts at: 3925 3925
Size: 6355
PspOMI G'GGCC_C
Cuts at: 2577 2577
Size: 6355
PspXI vC'TCGA_Gb
Cuts at: 3057 3057
Size: 6355
PstI C_TGCA'G
Cuts at: 830 2235 2427 3180 3617 3707 830
Size: 1405 192 753 437 90 3478
Fragments arranged by size:
3478 1405 753 437 192 90
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6355
PvuII CAG'CTG
Cuts at: 2428 2428
Size: 6355
SacII CC_GC'GG
Cuts at: 2320 2320
Size: 6355
SalI G'TCGA_C
Cuts at: 1936 3697 1936
Size: 1761 4594
SapI GCTCTTCn'nnn_
Cuts at: 2513 3015 2513
Size: 502 5853
SbfI CC_TGCA'GG
Cuts at: 3707 3707
Size: 6355
ScaI AGT'ACT
Cuts at: 4603 6315 4603
Size: 1712 4643
SfcI C'TryA_G
Cuts at: 826 1045 2231 2423 3176 3613 3703 4838 5519
Size: 219 1186 192 753 437 90 1135 681
Cuts at: 5519 5710 826
Size: 191 1471
Fragments arranged by size:
1471 1186 1135 753 681 437 219 192
191 90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6355
SmaI CCC'GGG
Cuts at: 1872 3682 1872
Size: 1810 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3057 4459 5360 5622 820
Size: 197 926 1114 1402 901 262 1553
Fragments arranged by size:
1553 1402 1114 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4146 493
Size: 3653 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6355
SphI G_CATG'C
Cuts at: 1132 3713 1132
Size: 2581 3774
SspI AAT'ATT
Cuts at: 5 52 2375 4279 5
Size: 47 2323 1904 2081
Fragments arranged by size:
2323 2081 1904 47
StuI AGG'CCT
Cuts at: 1517 3609 1517
Size: 2092 4263
StyI C'CwwG_G
Cuts at: 513 1065 3554 3794 513
Size: 552 2489 240 3074
Fragments arranged by size:
3074 2489 552 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4382 4399 4552 4737 5182 325
Size: 1255 2802 17 153 185 445 1498
Fragments arranged by size:
2802 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4601 6313 96
Size: 274 80 33 51 1443 2624 1712 138
Fragments arranged by size:
2624 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3182 3214 6315 6347 3182
Size: 32 3101 32 3190
Fragments arranged by size:
3190 3101 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6355
XbaI T'CTAG_A
Cuts at: 3691 3691
Size: 6355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2758 3480 2758
Size: 722 5633
XhoI C'TCGA_G
Cuts at: 1943 3057 1943
Size: 1114 5241
XmaI C'CCGG_G
Cuts at: 1870 3680 1870
Size: 1810 4545
XmnI GAAnn'nnTTC
Cuts at: 3520 4484 3520
Size: 964 5391
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4163 276
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PsrI RsrII SacI
SanDI SexAI SfiI SfoI SrfI SwaI