Restriction map for
pFN21AB5275
(Circular) (Six-base) MAPSORT of: pFN21AB5275.seq Check: 2870 from: 1 to: 6112
pFN21AB5275 6112 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3922 278
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3207 3455 1937
Size: 1270 248 4594
Fragments arranged by size:
4594 1270 248
Acc65I G'GTAC_C
Cuts at: 3433 3433
Size: 6112
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3280 4175 5559 5827 1632
Size: 1648 895 1384 268 1917
Fragments arranged by size:
1917 1648 1384 895 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 2451 5011 5988 1203
Size: 1248 2560 977 1327
Fragments arranged by size:
2560 1327 1248 977
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2669 1184
Size: 1485 4627
AlwNI CAG_nnn'CTG
Cuts at: 2656 3140 3855 5427 2656
Size: 484 715 1572 3341
Fragments arranged by size:
3341 1572 715 484
ApaI G_GGCC'C
Cuts at: 2035 2212 3410 2035
Size: 177 1198 4737
Fragments arranged by size:
4737 1198 177
ApaLI G'TGCA_C
Cuts at: 4169 5325 4169
Size: 1156 4956
ApoI r'AATT_y
Cuts at: 1493 3421 3647 3799 5908 1493
Size: 1928 226 152 2109 1697
Fragments arranged by size:
2109 1928 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 2079 2721 3361 3437 1870
Size: 73 136 642 640 76 4545
Fragments arranged by size:
4545 642 640 136 76 73
BamHI G'GATC_C
Cuts at: 3442 3809 3442
Size: 367 5745
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2083 2441 2645 3433 4888
Size: 325 217 627 296 358 204 788 1455
Cuts at: 4888 618
Size: 1842
Fragments arranged by size:
1842 1455 788 627 358 325 296 217
204
BanII G_rGCy'C
Cuts at: 1391 2035 2212 3410 3431 1391
Size: 644 177 1198 21 4072
Fragments arranged by size:
4072 1198 644 177 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5141
BbvCI CC'TCA_GC
Cuts at: 2747 2747
Size: 6112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4303 4337 1903
Size: 34 2366 34 3678
Fragments arranged by size:
3678 2366 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4004 5214 4004
Size: 1210 4902
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2030 2619 2643
Size: 107 122 71 631 605 358 589 24
Cuts at: 2643 4723 136
Size: 2080 1525
Fragments arranged by size:
2080 1525 631 605 589 358 122 107
71 24
BglII A'GATC_T
Cuts at: 6107 6107
Size: 6112
BlpI GC'TnA_GC
Cuts at: 2861 3529 2861
Size: 668 5444
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2212 2291 2648 2877 3200 3410 4173
Size: 245 177 79 357 229 323 210 763
Cuts at: 4173 5329 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 763 357 323 245 229 210
177 79
BmgBI CAC'GTC
Cuts at: 1318 2452 2716 2884 1318
Size: 1134 264 168 4546
Fragments arranged by size:
4546 1134 264 168
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2230 3843 4801
Size: 416 53 83 399 24 813 1613 958
Cuts at: 4801 442
Size: 1753
Fragments arranged by size:
1753 1613 958 813 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2963 3119 4772 1239
Size: 980 744 156 1653 2579
Fragments arranged by size:
2579 1653 980 744 156
Bpu10I CC'TnA_GC
Cuts at: 2747 2747
Size: 6112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4237 5102 5400 4237
Size: 865 298 4949
Fragments arranged by size:
4949 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2182 4775 882
Size: 617 683 2593 2219
Fragments arranged by size:
2593 2219 683 617
BsaAI yAC'GTr
Cuts at: 493 1204 3903 493
Size: 711 2699 2702
Fragments arranged by size:
2702 2699 711
BsaBI GATnn'nnATC
Cuts at: 6106 6106
Size: 6112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3919 4301 4936 275
Size: 53 83 186 936 2386 382 635 1451
Fragments arranged by size:
2386 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4545 4943 5217 5364 5775 4545
Size: 398 274 147 411 4882
Fragments arranged by size:
4882 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 2223 2253 1178
Size: 30 165 30 687 30 103 30 5037
Fragments arranged by size:
5037 687 165 103 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2098 2282 2357 2358 2735 1186
Size: 912 184 75 1 377 4563
Fragments arranged by size:
4563 912 377 184 75 1
BseYI C'CCAG_C
Cuts at: 2211 2361 2641 2649 2925 3227 3403 5315 5811
Size: 150 280 8 276 302 176 1912 496
Cuts at: 5811 2211
Size: 2512
Fragments arranged by size:
2512 1912 496 302 280 276 176 150
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2722 1163
Size: 573 986 4553
Fragments arranged by size:
4553 986 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3562 4323 5351 1540
Size: 275 186 1561 761 1028 2301
Fragments arranged by size:
2301 1561 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2349 4173 4258 5329 2349
Size: 1824 85 1071 3132
Fragments arranged by size:
3132 1824 1071 85
BsmI GAATG_Cn'
Cuts at: 3623 3716 3623
Size: 93 6019
BsmBI CGTCTCn'nnnn_
Cuts at: 2496 2722 2496
Size: 226 5886
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2212 2291 2349 2648 2877 3200
Size: 399 245 177 79 58 299 229 323
Cuts at: 3200 3410 3431 4173 4258 5329 1391
Size: 210 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 323 299 245 229
210 177 85 79 58 21
BspEI T'CCGG_A
Cuts at: 5775 5775
Size: 6112
BspHI T'CATG_A
Cuts at: 4001 5731 4001
Size: 1730 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2361 3336 3467 844
Size: 1517 975 131 3489
Fragments arranged by size:
3489 1517 975 131
BsrBI CCG'CTC
Cuts at: 1394 1823 3999 1394
Size: 429 2176 3507
Fragments arranged by size:
3507 2176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4607 4781 65
Size: 1183 3359 174 1396
Fragments arranged by size:
3359 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2145 4756 4943 1700
Size: 110 335 2611 187 2869
Fragments arranged by size:
2869 2611 335 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 4172 5184 4172
Size: 1012 5100
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2353 794
Size: 1559 4553
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 2986 1184
Size: 1802 4310
BstYI r'GATC_y
Cuts at: 1972 2847 3093 3442 3809 4194 4211 5663 5749
Size: 875 246 349 367 385 17 1452 86
Cuts at: 5749 5761 6107 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 875 385 367 349 346 246
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2265 3258 2265
Size: 993 5119
BtgI C'CryG_G
Cuts at: 513 1065 2877 513
Size: 552 1812 3748
Fragments arranged by size:
3748 1812 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5373
Fragments arranged by size:
5373 683 56
BtsI GCAGTG_nn'
Cuts at: 2233 2705 2961 3628 4422 4442 2233
Size: 472 256 667 794 20 3903
Fragments arranged by size:
3903 794 667 472 256 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6077
DraI TTT'AAA
Cuts at: 3416 3416
Size: 6112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2402 5119 809
Size: 507 1086 2717 1802
Fragments arranged by size:
2717 1802 1086 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2958 3559 4448
Size: 54 1425 50 275 18 1128 601 889
Cuts at: 4448 6077 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1128 889 601 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3559 1537
Size: 275 1747 4090
Fragments arranged by size:
4090 1747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4042 1353
Size: 2689 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4699 5073 5219 387
Size: 1064 138 3110 374 146 1280
Fragments arranged by size:
3110 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2963 3119 3280 4175 4772 5559
Size: 393 587 744 156 161 895 597 787
Cuts at: 5559 5827 1239
Size: 268 1524
Fragments arranged by size:
1524 895 787 744 597 587 393 268
161 156
EcoNI CCTnn'n_nnAGG
Cuts at: 2330 2330
Size: 6112
EcoO109I rG'GnC_Cy
Cuts at: 2031 3254 3407 2031
Size: 1223 153 4736
Fragments arranged by size:
4736 1223 153
EcoRI G'AATT_C
Cuts at: 3421 3799 3421
Size: 378 5734
FspI TGC'GCA
Cuts at: 3332 4618 3332
Size: 1286 4826
HaeII r_GCGC'y
Cuts at: 1678 2709 2868 5259 1678
Size: 1031 159 2391 2531
Fragments arranged by size:
2531 2391 1031 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4753 4785 4827 4859 1373
Size: 32 135 32 3181 32 42 32 2626
Fragments arranged by size:
3181 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3456 3702 669
Size: 988 281 1518 246 3079
Fragments arranged by size:
3079 1518 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6112
HpaI GTT'AAC
Cuts at: 3702 3702
Size: 6112
KpnI G_GTAC'C
Cuts at: 3437 3437
Size: 6112
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5226 5410 1305
Size: 3921 184 2007
Fragments arranged by size:
3921 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2960 10
Size: 54 1425 343 1128 3162
Fragments arranged by size:
3162 1425 1128 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2163 2183 2391 2450 4070 4429 4588
Size: 648 997 20 208 59 1620 359 159
Cuts at: 4588 518
Size: 2042
Fragments arranged by size:
2042 1620 997 648 359 208 159 59
20
MspA1I CmG'CkG
Cuts at: 1606 2311 2653 2860 2956 2983 3528 4205 5353
Size: 705 342 207 96 27 545 677 1148
Cuts at: 5353 5598 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 705 677 545 342 245 207
96 27
NaeI GCC'GGC
Cuts at: 2147 2147
Size: 6112
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5560
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NgoMIV G'CCGG_C
Cuts at: 2145 2145
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6112
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 2950 3206 3470 5015 1132
Size: 97 1721 256 264 1545 2229
Fragments arranged by size:
2229 1721 1545 264 256 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6112
PciI A'CATG_T
Cuts at: 5011 5011
Size: 6112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2656 1108
Size: 1548 4564
PfoI T'CCnGG_A
Cuts at: 2196 3096 2196
Size: 900 5212
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3254 3254
Size: 6112
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6112
PsiI TTA'TAA
Cuts at: 3682 3682
Size: 6112
PspOMI G'GGCC_C
Cuts at: 2031 2208 3406 2031
Size: 177 1198 4737
Fragments arranged by size:
4737 1198 177
PstI C_TGCA'G
Cuts at: 830 2262 2316 2424 2859 3464 830
Size: 1432 54 108 435 605 3478
Fragments arranged by size:
3478 1432 605 435 108 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
PvuII CAG'CTG
Cuts at: 2653 2860 2983 2653
Size: 207 123 5782
Fragments arranged by size:
5782 207 123
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SfcI C'TryA_G
Cuts at: 826 1045 2258 2312 2420 2855 3460 4595 5276
Size: 219 1213 54 108 435 605 1135 681
Cuts at: 5276 5467 826
Size: 191 1471
Fragments arranged by size:
1471 1213 1135 681 605 435 219 191
108 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 2081 2723 3439 1872
Size: 209 642 716 4545
Fragments arranged by size:
4545 716 642 209
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4216 5117 5379 820
Size: 197 926 2273 901 262 1553
Fragments arranged by size:
2273 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 3470 1132
Size: 2338 3774
SspI AAT'ATT
Cuts at: 5 52 4036 5
Size: 47 3984 2081
Fragments arranged by size:
3984 2081 47
StuI AGG'CCT
Cuts at: 1517 3396 1517
Size: 1879 4233
StyI C'CwwG_G
Cuts at: 513 1065 3551 513
Size: 552 2486 3074
Fragments arranged by size:
3074 2486 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2403 2606 2663 2774 4139 4156 4309
Size: 1255 823 203 57 111 1365 17 153
Cuts at: 4309 4494 4939 325
Size: 185 445 1498
Fragments arranged by size:
1498 1365 1255 823 445 203 185 153
111 57 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4358 6070 96
Size: 274 80 33 51 1443 2381 1712 138
Fragments arranged by size:
2381 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2103 2135 2223 2255 3033 3065 6072 6104 2103
Size: 32 88 32 778 32 3007 32 2111
Fragments arranged by size:
3007 2111 778 88 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6112
XbaI T'CTAG_A
Cuts at: 3448 3448
Size: 6112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2980 2980
Size: 6112
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 2079 2721 3437 1870
Size: 209 642 716 4545
Fragments arranged by size:
4545 716 642 209
XmnI GAAnn'nnTTC
Cuts at: 4241 4241
Size: 6112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3920 276
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI