Restriction map for
pFN21AB5272
(Circular) (Six-base) MAPSORT of: pFN21AB5272.seq Check: 5342 from: 1 to: 5449
pFN21AB5272 5449 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3259 278
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2792 1937
Size: 855 4594
Acc65I G'GTAC_C
Cuts at: 2770 2770
Size: 5449
AclI AA'CG_TT
Cuts at: 1563 3576 3949 1563
Size: 2013 373 3063
Fragments arranged by size:
3063 2013 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2507 3512 4896 5164 1632
Size: 875 1005 1384 268 1917
Fragments arranged by size:
1917 1384 1005 875 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5252
AflIII A'CryG_T
Cuts at: 1203 4348 5325 1203
Size: 3145 977 1327
Fragments arranged by size:
3145 1327 977
AgeI A'CCGG_T
Cuts at: 4280 4280
Size: 5449
AhdI GACnn_n'nnGTC
Cuts at: 4178 4178
Size: 5449
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5449
AlwNI CAG_nnn'CTG
Cuts at: 2053 2728 3192 4764 2053
Size: 675 464 1572 2738
Fragments arranged by size:
2738 1572 675 464
ApaI G_GGCC'C
Cuts at: 2128 2128
Size: 5449
ApaLI G'TGCA_C
Cuts at: 3506 4662 3506
Size: 1156 4293
ApoI r'AATT_y
Cuts at: 1493 2758 2984 3136 5245 1493
Size: 1265 226 152 2109 1697
Fragments arranged by size:
2109 1697 1265 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4003 160
Size: 872 2971 1606
Fragments arranged by size:
2971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
AvaI C'yCGr_G
Cuts at: 1870 1943 2127 2774 1870
Size: 73 184 647 4545
Fragments arranged by size:
4545 647 184 73
BamHI G'GATC_C
Cuts at: 2169 2779 3146 2169
Size: 610 367 4472
Fragments arranged by size:
4472 610 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2770 4225 618
Size: 325 217 627 983 1455 1842
Fragments arranged by size:
1842 1455 983 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2128 2768 1391
Size: 737 640 4072
Fragments arranged by size:
4072 737 640
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4478
BbvCI CC'TCA_GC
Cuts at: 2139 2145 2139
Size: 6 5443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3640 3674 1903
Size: 34 1703 34 3678
Fragments arranged by size:
3678 1703 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3341 4551 3341
Size: 1210 4239
BclI T'GATC_A
Cuts at: 1548 2406 1548
Size: 858 4591
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4060 136
Size: 107 122 71 631 605 2388 1525
Fragments arranged by size:
2388 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5444 5444
Size: 5449
BlpI GC'TnA_GC
Cuts at: 2088 2349 2445 2866 2088
Size: 261 96 421 4671
Fragments arranged by size:
4671 421 261 96
Bme1580I G_kGCm'C
Cuts at: 1790 2128 3510 4666 1790
Size: 338 1382 1156 2573
Fragments arranged by size:
2573 1382 1156 338
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5449
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3180 4138 442
Size: 416 53 83 399 24 1763 958 1753
Fragments arranged by size:
1763 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5449
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2170 2554 2170
Size: 384 5065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2309 2495 4109 1239
Size: 842 228 186 1614 2579
Fragments arranged by size:
2579 1614 842 228 186
Bpu10I CC'TnA_GC
Cuts at: 2139 2145 2202 2523 2139
Size: 6 57 321 5065
Fragments arranged by size:
5065 321 57 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2492 2537 2576 3574 4439 4737 2492
Size: 45 39 998 865 298 3204
Fragments arranged by size:
3204 998 865 298 45 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2597 4112 882
Size: 617 1098 1515 2219
Fragments arranged by size:
2219 1515 1098 617
BsaAI yAC'GTr
Cuts at: 493 1204 3240 493
Size: 711 2036 2702
Fragments arranged by size:
2702 2036 711
BsaBI GATnn'nnATC
Cuts at: 5443 5443
Size: 5449
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3256 3638 4273 275
Size: 53 83 186 936 1723 382 635 1451
Fragments arranged by size:
1723 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3882 4280 4554 4701 5112 3882
Size: 398 274 147 411 4219
Fragments arranged by size:
4219 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5224
Fragments arranged by size:
5224 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2492 2495 2705 1186
Size: 907 399 3 210 3930
Fragments arranged by size:
3930 907 399 210 3
BseYI C'CCAG_C
Cuts at: 2009 4652 5148 2009
Size: 2643 496 2310
Fragments arranged by size:
2643 2310 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4876
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2899 3660 4688 1540
Size: 275 186 898 761 1028 2301
Fragments arranged by size:
2301 1028 898 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2203 2697 3510 3595 4666 2203
Size: 494 813 85 1071 2986
Fragments arranged by size:
2986 1071 813 494 85
BsmI GAATG_Cn'
Cuts at: 2960 3053 2960
Size: 93 5356
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 2203 2697 2768 3510 3595 4666
Size: 399 338 75 494 71 742 85 1071
Cuts at: 4666 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 494 399 338 85 75
71
BspEI T'CCGG_A
Cuts at: 5112 5112
Size: 5449
BspHI T'CATG_A
Cuts at: 3338 5068 3338
Size: 1730 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2804 844
Size: 1960 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3336 1394
Size: 429 1513 3507
Fragments arranged by size:
3507 1513 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2355 3944 4118 65
Size: 1183 1107 1589 174 1396
Fragments arranged by size:
1589 1396 1183 1107 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4093 4280 1700
Size: 110 2283 187 2869
Fragments arranged by size:
2869 2283 187 110
BsrGI T'GTAC_A
Cuts at: 96 2702 96
Size: 2606 2843
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5449
BssSI C'ACGA_G
Cuts at: 3509 4521 3509
Size: 1012 4437
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2072 794
Size: 1278 4171
BstBI TT'CG_AA
Cuts at: 4253 4253
Size: 5449
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5449
BstYI r'GATC_y
Cuts at: 1972 2043 2169 2779 3146 3531 3548 5000 5086
Size: 71 126 610 367 385 17 1452 86
Cuts at: 5086 5098 5444 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 610 385 367 346 126 86
71 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3958
Fragments arranged by size:
3958 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4710
Fragments arranged by size:
4710 683 56
BtsI GCAGTG_nn'
Cuts at: 2217 2965 3759 3779 2217
Size: 748 794 20 3887
Fragments arranged by size:
3887 794 748 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5414
DraI TTT'AAA
Cuts at: 2753 2753
Size: 5449
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4456 809
Size: 507 3140 1802
Fragments arranged by size:
3140 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 2896 3785
Size: 54 1425 50 275 18 384 682 889
Cuts at: 3785 5414 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 682 384 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2896 1537
Size: 275 1084 4090
Fragments arranged by size:
4090 1084 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2181 2358 2379 3379 1353
Size: 828 177 21 1000 3423
Fragments arranged by size:
3423 1000 828 177 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2543 4036 4410 4556 387
Size: 1064 138 954 1493 374 146 1280
Fragments arranged by size:
1493 1280 1064 954 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2309 2495 2507 3512 4109 4896
Size: 393 449 228 186 12 1005 597 787
Cuts at: 4896 5164 1239
Size: 268 1524
Fragments arranged by size:
1524 1005 787 597 449 393 268 228
186 12
EcoRI G'AATT_C
Cuts at: 2758 3136 2758
Size: 378 5071
FspI TGC'GCA
Cuts at: 3955 3955
Size: 5449
HaeII r_GCGC'y
Cuts at: 1678 2095 2212 4596 1678
Size: 417 117 2384 2531
Fragments arranged by size:
2531 2384 417 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4090 4122 4164 4196 1373
Size: 32 135 32 2518 32 42 32 2626
Fragments arranged by size:
2626 2518 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2793 3039 669
Size: 988 281 855 246 3079
Fragments arranged by size:
3079 988 855 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5449
HpaI GTT'AAC
Cuts at: 3039 3039
Size: 5449
KpnI G_GTAC'C
Cuts at: 2774 2774
Size: 5449
MfeI C'AATT_G
Cuts at: 2652 3048 2652
Size: 396 5053
MluI A'CGCG_T
Cuts at: 5325 5325
Size: 5449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2359 4563 4747 1305
Size: 1054 2204 184 2007
Fragments arranged by size:
2204 2007 1054 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2216 10
Size: 54 1425 343 384 3243
Fragments arranged by size:
3243 1425 384 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3407 3766 3925 518
Size: 648 2241 359 159 2042
Fragments arranged by size:
2241 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2176 2865 3542 4690 4935 1606
Size: 570 689 677 1148 245 2120
Fragments arranged by size:
2120 1148 689 677 570 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3958
Fragments arranged by size:
3958 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5449
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5449
NotI GC'GGCC_GC
Cuts at: 2896 2896
Size: 5449
NspI r_CATG'y
Cuts at: 1132 1229 2807 4352 1132
Size: 97 1578 1545 2229
Fragments arranged by size:
2229 1578 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5449
PciI A'CATG_T
Cuts at: 4348 4348
Size: 5449
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5449
PfoI T'CCnGG_A
Cuts at: 2325 2325
Size: 5449
PmeI GTTT'AAAC
Cuts at: 2753 2753
Size: 5449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 5031 5063 3518
Size: 32 1481 32 3904
Fragments arranged by size:
3904 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5449
PsiI TTA'TAA
Cuts at: 3019 3019
Size: 5449
PspOMI G'GGCC_C
Cuts at: 2124 2124
Size: 5449
PstI C_TGCA'G
Cuts at: 830 2058 2801 830
Size: 1228 743 3478
Fragments arranged by size:
3478 1228 743
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5449
PvuII CAG'CTG
Cuts at: 2176 2176
Size: 5449
SalI G'TCGA_C
Cuts at: 1936 2791 1936
Size: 855 4594
SapI GCTCTTCn'nnn_
Cuts at: 2181 2181
Size: 5449
SbfI CC_TGCA'GG
Cuts at: 2801 2801
Size: 5449
ScaI AGT'ACT
Cuts at: 2537 3697 5409 2537
Size: 1160 1712 2577
Fragments arranged by size:
2577 1712 1160
SfcI C'TryA_G
Cuts at: 826 1045 2054 2797 3932 4613 4804 826
Size: 219 1009 743 1135 681 191 1471
Fragments arranged by size:
1471 1135 1009 743 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5449
SmaI CCC'GGG
Cuts at: 1872 2129 2776 1872
Size: 257 647 4545
Fragments arranged by size:
4545 647 257
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2507 2516 2591 3553 4454 4716
Size: 197 926 564 9 75 962 901 262
Cuts at: 4716 820
Size: 1553
Fragments arranged by size:
1553 962 926 901 564 262 197 75
9
SnaBI TAC'GTA
Cuts at: 493 3240 493
Size: 2747 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5449
SphI G_CATG'C
Cuts at: 1132 2807 1132
Size: 1675 3774
SspI AAT'ATT
Cuts at: 5 52 3373 5
Size: 47 3321 2081
Fragments arranged by size:
3321 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5449
StyI C'CwwG_G
Cuts at: 513 1065 2004 2888 513
Size: 552 939 884 3074
Fragments arranged by size:
3074 939 884 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3476 3493 3646 3831 4276 325
Size: 1255 1896 17 153 185 445 1498
Fragments arranged by size:
1896 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2535 2702 3695
Size: 274 80 33 51 1443 558 167 993
Cuts at: 3695 5407 96
Size: 1712 138
Fragments arranged by size:
1712 1443 993 558 274 167 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5409 5441 5409
Size: 32 5417
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5449
XbaI T'CTAG_A
Cuts at: 2785 2785
Size: 5449
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5449
XmaI C'CCGG_G
Cuts at: 1870 2127 2774 1870
Size: 257 647 4545
Fragments arranged by size:
4545 647 257
XmnI GAAnn'nnTTC
Cuts at: 3578 3578
Size: 5449
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3257 276
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI