Restriction map for
pFN21AB5271
(Circular) (Six-base) MAPSORT of: pFN21AB5271.seq Check: 6068 from: 1 to: 5956
pFN21AB5271 5956 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3052 3052
Size: 5956
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3154 3299 1937
Size: 1217 145 4594
Fragments arranged by size:
4594 1217 145
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2290 2684 4019 5403 5671 1632
Size: 658 394 1335 1384 268 1917
Fragments arranged by size:
1917 1384 1335 658 394 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2859 1184
Size: 1675 4281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2457 2489 2457
Size: 32 5924
AlwNI CAG_nnn'CTG
Cuts at: 3699 5271 3699
Size: 1572 4384
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 2213 2693 2808 2908 3030 3265 3491 3643
Size: 720 480 115 100 122 235 226 152
Cuts at: 3643 5752 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 720 480 235 226 152 122
115 100
AseI AT'TA_AT
Cuts at: 160 1032 2403 4510 160
Size: 872 1371 2107 1606
Fragments arranged by size:
2107 1606 1371 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 3281 1870
Size: 73 1338 4545
Fragments arranged by size:
4545 1338 73
AvrII C'CTAG_G
Cuts at: 2500 2500
Size: 5956
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3277 4732 618
Size: 325 217 627 1490 1455 1842
Fragments arranged by size:
1842 1490 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3275 1391
Size: 1884 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2048 928
Size: 971 149 4836
Fragments arranged by size:
4836 971 149
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4147 4181 1903
Size: 34 50 34 2126 34 3678
Fragments arranged by size:
3678 2126 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4567 136
Size: 107 122 71 631 605 2895 1525
Fragments arranged by size:
2895 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 4017 5173 1790
Size: 2227 1156 2573
Fragments arranged by size:
2573 2227 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2465 3687 4645
Size: 416 53 83 399 24 1048 1222 958
Cuts at: 4645 442
Size: 1753
Fragments arranged by size:
1753 1222 1048 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2333 2740 2333
Size: 407 5549
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2115 4616 1239
Size: 876 2501 2579
Fragments arranged by size:
2579 2501 876
Bpu10I CC'TnA_GC
Cuts at: 2151 2151
Size: 5956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 4946 5244 4081
Size: 865 298 4793
Fragments arranged by size:
4793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2253 4619 882
Size: 617 754 2366 2219
Fragments arranged by size:
2366 2219 754 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3763 4145 4780 275
Size: 53 83 186 936 2230 382 635 1451
Fragments arranged by size:
2230 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2611 4389 4787 5061 5208 5619 2611
Size: 1778 398 274 147 411 2948
Fragments arranged by size:
2948 1778 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5731
Fragments arranged by size:
5731 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5956
BseYI C'CCAG_C
Cuts at: 2141 5159 5655 2141
Size: 3018 496 2442
Fragments arranged by size:
3018 2442 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5383
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2052 3406 4167 5195 1540
Size: 275 186 51 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186 51
BsiHKAI G_wGCw'C
Cuts at: 2494 4017 4102 5173 2494
Size: 1523 85 1071 3277
Fragments arranged by size:
3277 1523 1071 85
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2494 3275 4017 4102 5173 1391
Size: 399 704 781 742 85 1071 2174
Fragments arranged by size:
2174 1071 781 742 704 399 85
BspEI T'CCGG_A
Cuts at: 2611 5619 2611
Size: 3008 2948
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3052 3311 844
Size: 2208 259 3489
Fragments arranged by size:
3489 2208 259
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 2031 3843 1394
Size: 429 188 20 1812 3507
Fragments arranged by size:
3507 1812 429 188 20
BsrDI GCAATG_nn'
Cuts at: 65 1248 4451 4625 65
Size: 1183 3203 174 1396
Fragments arranged by size:
3203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4600 4787 1700
Size: 110 2790 187 2869
Fragments arranged by size:
2869 2790 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 2322 4016 5028 2322
Size: 1694 1012 3250
Fragments arranged by size:
3250 1694 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 3028 4760 3028
Size: 1732 4224
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstYI r'GATC_y
Cuts at: 1972 3286 3653 4038 4055 5507 5593 5605 5951
Size: 1314 367 385 17 1452 86 12 346
Cuts at: 5951 1972
Size: 1977
Fragments arranged by size:
1977 1452 1314 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3155 3155
Size: 5956
BtgI C'CryG_G
Cuts at: 513 1065 2412 513
Size: 552 1347 4057
Fragments arranged by size:
4057 1347 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 3472 4266 4286 3472
Size: 794 20 5142
Fragments arranged by size:
5142 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3403 4292 5921
Size: 54 1425 50 275 18 1573 889 1629
Cuts at: 5921 8
Size: 43
Fragments arranged by size:
1629 1573 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2391 2520 2826 3227 3886 1353
Size: 1038 129 306 401 659 3423
Fragments arranged by size:
3423 1038 659 401 306 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4543 4917 5063 387
Size: 1064 138 2954 374 146 1280
Fragments arranged by size:
2954 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2290 2684 4019 4616 5403 5671
Size: 393 483 175 394 1335 597 787 268
Cuts at: 5671 1239
Size: 1524
Fragments arranged by size:
1524 1335 787 597 483 394 393 268
175
EcoNI CCTnn'n_nnAGG
Cuts at: 2066 2066
Size: 5956
EcoO109I rG'GnC_Cy
Cuts at: 2020 2020
Size: 5956
EcoRI G'AATT_C
Cuts at: 2213 3030 3265 3643 2213
Size: 817 235 378 4526
Fragments arranged by size:
4526 817 378 235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3122 3122
Size: 5956
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 5103 1678
Size: 3425 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2305 2336 2337 2368 2664
Size: 32 135 32 733 31 1 31 296
Cuts at: 2664 2696 4597 4629 4671 4703 1373
Size: 32 1901 32 42 32 2626
Fragments arranged by size:
2626 1901 733 296 135 42 32 32
32 32 32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2560 2743 3300 3546 669
Size: 988 281 622 183 557 246 3079
Fragments arranged by size:
3079 988 622 557 281 246 183
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2508 5070 5254 1305
Size: 1203 2562 184 2007
Fragments arranged by size:
2562 2007 1203 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4134
Fragments arranged by size:
4134 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2933 3914 4273 4432 518
Size: 648 1767 981 359 159 2042
Fragments arranged by size:
2042 1767 981 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3372 4049 5197 5442 1606
Size: 1766 677 1148 245 2120
Fragments arranged by size:
2120 1766 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2412 513
Size: 552 1347 4057
Fragments arranged by size:
4057 1347 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 3314 4859 1132
Size: 97 2085 1545 2229
Fragments arranged by size:
2229 2085 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2756 1108
Size: 1648 4308
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2048 1613
Size: 435 5521
PsiI TTA'TAA
Cuts at: 3138 3526 3138
Size: 388 5568
PstI C_TGCA'G
Cuts at: 830 3308 830
Size: 2478 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SapI GCTCTTCn'nnn_
Cuts at: 2391 2520 2826 2391
Size: 129 306 5521
Fragments arranged by size:
5521 306 129
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SfcI C'TryA_G
Cuts at: 826 1045 3304 4439 5120 5311 826
Size: 219 2259 1135 681 191 1471
Fragments arranged by size:
2259 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4060 4961 5223 820
Size: 197 926 2117 901 262 1553
Fragments arranged by size:
2117 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 2339 2701 2856 3134 3880 5
Size: 47 2287 362 155 278 746 2081
Fragments arranged by size:
2287 2081 746 362 278 155 47
StuI AGG'CCT
Cuts at: 1517 2072 1517
Size: 555 5401
StyI C'CwwG_G
Cuts at: 513 1065 2412 2500 2779 3395 513
Size: 552 1347 88 279 616 3074
Fragments arranged by size:
3074 1347 616 552 279 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3983 4000 4153 4338 4783 325
Size: 1255 2403 17 153 185 445 1498
Fragments arranged by size:
2403 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2552 4202 5914
Size: 274 80 33 51 1443 575 1650 1712
Cuts at: 5914 96
Size: 138
Fragments arranged by size:
1712 1650 1443 575 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5916 5948 5916
Size: 32 5924
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2854 2854
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 2404 4085 2404
Size: 1681 4275
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstXI Bsu36I DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI