Restriction map for
pFN21AB5266
(Circular) (Six-base) MAPSORT of: pFN21AB5266.seq Check: 4696 from: 1 to: 5755
pFN21AB5266 5755 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2039 2039
Size: 5755
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2053 2421 2949 2991 3018 3818 5202 5470
Size: 421 368 528 42 27 800 1384 268
Cuts at: 5470 1632
Size: 1917
Fragments arranged by size:
1917 1384 800 528 421 368 268 42
27
AfeI AGC'GCT
Cuts at: 2107 2107
Size: 5755
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AlwNI CAG_nnn'CTG
Cuts at: 2237 3041 3498 5070 2237
Size: 804 457 1572 2922
Fragments arranged by size:
2922 1572 804 457
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 2545 2711 3013 3064 3290 3442 5551 1493
Size: 1052 166 302 51 226 152 2109 1697
Fragments arranged by size:
2109 1697 1052 302 226 166 152 51
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 3080 1870
Size: 73 1137 4545
Fragments arranged by size:
4545 1137 73
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3076 4531 618
Size: 325 217 627 1289 1455 1842
Fragments arranged by size:
1842 1455 1289 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3074 1391
Size: 1683 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4784
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3946 3980 1903
Size: 34 2009 34 3678
Fragments arranged by size:
3678 2009 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4366 136
Size: 107 122 71 631 605 2694 1525
Fragments arranged by size:
2694 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 3816 4972 1790
Size: 2026 1156 2573
Fragments arranged by size:
2573 2026 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4415 1239
Size: 3176 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3880 4745 5043 3880
Size: 865 298 4592
Fragments arranged by size:
4592 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 3562 3944 4579
Size: 53 83 186 936 488 1541 382 635
Cuts at: 4579 275
Size: 1451
Fragments arranged by size:
1541 1451 936 635 488 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5530
Fragments arranged by size:
5530 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3002 1186
Size: 1816 3939
BseYI C'CCAG_C
Cuts at: 4958 5454 4958
Size: 496 5259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2479 2655 1163
Size: 573 743 176 4263
Fragments arranged by size:
4263 743 573 176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3816 3901 4972 3816
Size: 85 1071 4599
Fragments arranged by size:
4599 1071 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3074 3816 3901 4972 1391
Size: 399 1284 742 85 1071 2174
Fragments arranged by size:
2174 1284 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2039 3110 844
Size: 1195 1071 3489
Fragments arranged by size:
3489 1195 1071
BsrBI CCG'CTC
Cuts at: 1394 1823 2026 3642 1394
Size: 429 203 1616 3507
Fragments arranged by size:
3507 1616 429 203
BsrDI GCAATG_nn'
Cuts at: 65 1248 2016 4250 4424 65
Size: 1183 768 2234 174 1396
Fragments arranged by size:
2234 1396 1183 768 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4399 4586 1700
Size: 110 2589 187 2869
Fragments arranged by size:
2869 2589 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 3085 3452 3837 3854 5306 5392 5404 5750
Size: 1113 367 385 17 1452 86 12 346
Cuts at: 5750 1972
Size: 1977
Fragments arranged by size:
1977 1452 1113 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2412 2583 2412
Size: 171 5584
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 513
Size: 552 939 48 4216
Fragments arranged by size:
4216 939 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 2467 3271 4065 4085 2467
Size: 804 794 20 4137
Fragments arranged by size:
4137 804 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 2740 3059 2740
Size: 319 5436
DraIII CAC_nnn'GTG
Cuts at: 2815 2815
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2799 4762 809
Size: 507 1483 1963 1802
Fragments arranged by size:
1963 1802 1483 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2038 3202 4091
Size: 54 1425 50 275 18 208 1164 889
Cuts at: 4091 5720 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1164 889 275 208 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2700 2979 3685 1353
Size: 1347 279 706 3423
Fragments arranged by size:
3423 1347 706 279
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2421 2949 2991 3018 3818 4415
Size: 393 421 368 528 42 27 800 597
Cuts at: 4415 5202 5470 1239
Size: 787 268 1524
Fragments arranged by size:
1524 800 787 597 528 421 393 368
268 42 27
EcoRI G'AATT_C
Cuts at: 3013 3064 3442 3013
Size: 51 378 5326
Fragments arranged by size:
5326 378 51
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2732 2812 2732
Size: 80 5675
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 2109 4902 1678
Size: 431 2793 2531
Fragments arranged by size:
2793 2531 431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2281 2287 2313 2809
Size: 32 135 32 683 26 6 26 496
Cuts at: 2809 2841 4396 4428 4470 4502 1373
Size: 32 1555 32 42 32 2626
Fragments arranged by size:
2626 1555 683 496 135 42 32 32
32 32 32 26 26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2352 3099 3345 669
Size: 988 281 414 747 246 3079
Fragments arranged by size:
3079 988 747 414 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 2352 3345 2352
Size: 993 4762
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4869 5053 1305
Size: 3564 184 2007
Fragments arranged by size:
3564 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2040 10
Size: 54 1425 343 208 3725
Fragments arranged by size:
3725 1425 343 208 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2181 3713 4072 4231 518
Size: 648 843 172 1532 359 159 2042
Fragments arranged by size:
2042 1532 843 648 359 172 159
MspA1I CmG'CkG
Cuts at: 1606 2237 3171 3848 4996 5241 1606
Size: 631 934 677 1148 245 2120
Fragments arranged by size:
2120 1148 934 677 631 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4264
Fragments arranged by size:
4264 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 3113 4658 1132
Size: 97 1884 1545 2229
Fragments arranged by size:
2229 1884 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2259 2963 1108
Size: 1151 704 3900
Fragments arranged by size:
3900 1151 704
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 2603 2748 2760 3325 2603
Size: 145 12 565 5033
Fragments arranged by size:
5033 565 145 12
PstI C_TGCA'G
Cuts at: 830 2242 3107 830
Size: 1412 865 3478
Fragments arranged by size:
3478 1412 865
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
PvuII CAG'CTG
Cuts at: 2237 2237
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2229 2238 3103 4238 4919 5110 826
Size: 219 1184 9 865 1135 681 191 1471
Fragments arranged by size:
1471 1184 1135 865 681 219 191 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3859 4760 5022 820
Size: 197 926 1916 901 262 1553
Fragments arranged by size:
1916 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2004 3194 513
Size: 552 939 1190 3074
Fragments arranged by size:
3074 1190 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3782 3799 3952 4137 4582 325
Size: 1255 2202 17 153 185 445 1498
Fragments arranged by size:
2202 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4001 5713 96
Size: 274 80 33 51 1443 2024 1712 138
Fragments arranged by size:
2024 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5715 5747 5715
Size: 32 5723
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 2577 3884 2577
Size: 1307 4448
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI