Restriction map for
pFN21AB5261
(Circular) (Six-base) MAPSORT of: pFN21AB5261.seq Check: 627 from: 1 to: 5311
pFN21AB5261 5311 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2516 2516
Size: 5311
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3121 278
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2412 2469 2654 1937
Size: 475 57 185 4594
Fragments arranged by size:
4594 475 185 57
Acc65I G'GTAC_C
Cuts at: 2316 2325 2632 2316
Size: 9 307 4995
Fragments arranged by size:
4995 307 9
AclI AA'CG_TT
Cuts at: 1563 3438 3811 1563
Size: 1875 373 3063
Fragments arranged by size:
3063 1875 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2355 3374 4758 5026 1632
Size: 723 1019 1384 268 1917
Fragments arranged by size:
1917 1384 1019 723 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5114
AflIII A'CryG_T
Cuts at: 1203 4210 5187 1203
Size: 3007 977 1327
Fragments arranged by size:
3007 1327 977
AgeI A'CCGG_T
Cuts at: 4142 4142
Size: 5311
AhdI GACnn_n'nnGTC
Cuts at: 4040 4040
Size: 5311
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5311
AlwNI CAG_nnn'CTG
Cuts at: 3054 4626 3054
Size: 1572 3739
ApaI G_GGCC'C
Cuts at: 2588 2588
Size: 5311
ApaLI G'TGCA_C
Cuts at: 3368 4524 3368
Size: 1156 4155
ApoI r'AATT_y
Cuts at: 1493 2620 2846 2998 5107 1493
Size: 1127 226 152 2109 1697
Fragments arranged by size:
2109 1697 1127 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3865 160
Size: 872 2833 1606
Fragments arranged by size:
2833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
AvaI C'yCGr_G
Cuts at: 1870 1943 2059 2516 2636 1870
Size: 73 116 457 120 4545
Fragments arranged by size:
4545 457 120 116 73
BamHI G'GATC_C
Cuts at: 2641 3008 2641
Size: 367 4944
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2073 2136 2316 2325
Size: 325 217 627 267 19 63 180 9
Cuts at: 2325 2567 2632 4087 618
Size: 242 65 1455 1842
Fragments arranged by size:
1842 1455 627 325 267 242 217 180
65 63 19 9
BanII G_rGCy'C
Cuts at: 1391 2588 2630 1391
Size: 1197 42 4072
Fragments arranged by size:
4072 1197 42
BbeI G_GCGC'C
Cuts at: 2058 2077 2140 2571 2058
Size: 19 63 431 4798
Fragments arranged by size:
4798 431 63 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3502 3536 1903
Size: 34 50 34 1481 34 3678
Fragments arranged by size:
3678 1481 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3203 4413 3203
Size: 1210 4101
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2063 3922 136
Size: 107 122 71 631 605 391 1859 1525
Fragments arranged by size:
1859 1525 631 605 391 122 107 71
BglII A'GATC_T
Cuts at: 5306 5306
Size: 5311
BlpI GC'TnA_GC
Cuts at: 2728 2728
Size: 5311
Bme1580I G_kGCm'C
Cuts at: 1790 2588 3372 4528 1790
Size: 798 784 1156 2573
Fragments arranged by size:
2573 1156 798 784
BmgBI CAC'GTC
Cuts at: 1318 2389 1318
Size: 1071 4240
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3042 4000 442
Size: 416 53 83 399 24 1625 958 1753
Fragments arranged by size:
1753 1625 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2533 3971 1239
Size: 902 392 1438 2579
Fragments arranged by size:
2579 1438 902 392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3436 4301 4599 3436
Size: 865 298 4148
Fragments arranged by size:
4148 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3974 882
Size: 617 2475 2219
Fragments arranged by size:
2475 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3102 493
Size: 711 1898 2702
Fragments arranged by size:
2702 1898 711
BsaBI GATnn'nnATC
Cuts at: 5305 5305
Size: 5311
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 2074 2137 2568
Size: 53 83 186 936 522 19 63 431
Cuts at: 2568 3118 3500 4135 275
Size: 550 382 635 1451
Fragments arranged by size:
1451 936 635 550 522 431 382 186
83 63 53 19
BsaWI w'CCGG_w
Cuts at: 3744 4142 4416 4563 4974 3744
Size: 398 274 147 411 4081
Fragments arranged by size:
4081 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 2282 2312 1178
Size: 30 165 30 711 30 138 30 4177
Fragments arranged by size:
4177 711 165 138 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2396 1186
Size: 940 270 4101
Fragments arranged by size:
4101 940 270
BseYI C'CCAG_C
Cuts at: 2043 2182 2581 4514 5010 2043
Size: 139 399 1933 496 2344
Fragments arranged by size:
2344 1933 496 399 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2488 1163
Size: 573 752 3986
Fragments arranged by size:
3986 752 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2087 2108 2148 2242 2255 2461
Size: 275 186 86 21 40 94 13 206
Cuts at: 2461 2761 3522 4550 1540
Size: 300 761 1028 2301
Fragments arranged by size:
2301 1028 761 300 275 206 186 94
86 40 21 13
BsiHKAI G_wGCw'C
Cuts at: 2157 3372 3457 4528 2157
Size: 1215 85 1071 2940
Fragments arranged by size:
2940 1215 1071 85
BsmI GAATG_Cn'
Cuts at: 2822 2915 2822
Size: 93 5218
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2588 2630 3372 3457 4528 1391
Size: 399 367 431 42 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 431 399 367 85 42
BspEI T'CCGG_A
Cuts at: 4974 4974
Size: 5311
BspHI T'CATG_A
Cuts at: 3200 4930 3200
Size: 1730 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2274 2337 2516 2666 844
Size: 1430 63 179 150 3489
Fragments arranged by size:
3489 1430 179 150 63
BsrBI CCG'CTC
Cuts at: 1394 1823 3198 1394
Size: 429 1375 3507
Fragments arranged by size:
3507 1375 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3806 3980 65
Size: 1183 2558 174 1396
Fragments arranged by size:
2558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 2103 2147 2453 3955 4142 1700
Size: 110 205 88 44 306 1502 187 2869
Fragments arranged by size:
2869 1502 306 205 187 110 88 44
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5311
BssHII G'CGCG_C
Cuts at: 1925 2201 1925
Size: 276 5035
BssSI C'ACGA_G
Cuts at: 3371 4383 3371
Size: 1012 4299
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5311
BstBI TT'CG_AA
Cuts at: 4115 4115
Size: 5311
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5311
BstXI CCAn_nnnn'nTGG
Cuts at: 2497 2497
Size: 5311
BstYI r'GATC_y
Cuts at: 1972 2641 3008 3393 3410 4862 4948 4960 5306
Size: 669 367 385 17 1452 86 12 346
Cuts at: 5306 1972
Size: 1977
Fragments arranged by size:
1977 1452 669 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2049 2087 2496 513
Size: 552 984 38 409 3328
Fragments arranged by size:
3328 984 552 409 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4572
Fragments arranged by size:
4572 683 56
BtsI GCAGTG_nn'
Cuts at: 2827 3621 3641 2827
Size: 794 20 4497
Fragments arranged by size:
4497 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5276
DraI TTT'AAA
Cuts at: 2615 2615
Size: 5311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4318 809
Size: 507 3002 1802
Fragments arranged by size:
3002 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2084 2105 2145
Size: 54 1425 50 275 18 254 21 40
Cuts at: 2145 2252 2458 2758 3647 5276 8
Size: 107 206 300 889 1629 43
Fragments arranged by size:
1629 1425 889 300 275 254 206 107
54 50 43 40 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2084 2105 2145 2252 2458 2758 1537
Size: 275 272 21 40 107 206 300 4090
Fragments arranged by size:
4090 300 275 272 206 107 40 21
EarI CTCTTCn'nnn_
Cuts at: 1353 3241 1353
Size: 1888 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3898 4272 4418 387
Size: 1064 138 2309 374 146 1280
Fragments arranged by size:
2309 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2355 2533 3374 3971 4758 5026
Size: 393 509 214 178 841 597 787 268
Cuts at: 5026 1239
Size: 1524
Fragments arranged by size:
1524 841 787 597 509 393 268 214
178
EcoO109I rG'GnC_Cy
Cuts at: 2585 2585
Size: 5311
EcoRI G'AATT_C
Cuts at: 2620 2998 2620
Size: 378 4933
FspI TGC'GCA
Cuts at: 2270 3817 2270
Size: 1547 3764
HaeII r_GCGC'y
Cuts at: 1678 2033 2058 2077 2140 2406 2571 4458 1678
Size: 355 25 19 63 266 165 1887 2531
Fragments arranged by size:
2531 1887 355 266 165 63 25 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3952 3984 4026 4058 1373
Size: 32 135 32 2380 32 42 32 2626
Fragments arranged by size:
2626 2380 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2655 2901 669
Size: 988 281 717 246 3079
Fragments arranged by size:
3079 988 717 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5311
HpaI GTT'AAC
Cuts at: 2901 2901
Size: 5311
KasI G'GCGC_C
Cuts at: 2054 2073 2136 2567 2054
Size: 19 63 431 4798
Fragments arranged by size:
4798 431 63 19
KpnI G_GTAC'C
Cuts at: 2320 2329 2636 2320
Size: 9 307 4995
Fragments arranged by size:
4995 307 9
MfeI C'AATT_G
Cuts at: 2910 2910
Size: 5311
MluI A'CGCG_T
Cuts at: 5187 5187
Size: 5311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4425 4609 1305
Size: 3120 184 2007
Fragments arranged by size:
3120 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3489
Fragments arranged by size:
3489 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 3269 3628 3787 518
Size: 648 1329 774 359 159 2042
Fragments arranged by size:
2042 1329 774 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2089 2128 2182 2603 2727 3404 4552
Size: 445 38 39 54 421 124 677 1148
Cuts at: 4552 4797 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 445 421 245 124 54
39 38
NaeI GCC'GGC
Cuts at: 2017 2149 2455 2017
Size: 132 306 4873
Fragments arranged by size:
4873 306 132
NarI GG'CG_CC
Cuts at: 2055 2074 2137 2568 2055
Size: 19 63 431 4798
Fragments arranged by size:
4798 431 63 19
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4759
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5311
NgoMIV G'CCGG_C
Cuts at: 2015 2147 2453 2015
Size: 132 306 4873
Fragments arranged by size:
4873 306 132
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5311
NotI GC'GGCC_GC
Cuts at: 2084 2758 2084
Size: 674 4637
NspI r_CATG'y
Cuts at: 1132 1229 2419 2669 4214 1132
Size: 97 1190 250 1545 2229
Fragments arranged by size:
2229 1545 1190 250 97
PasI CC'CwG_GG
Cuts at: 1348 2283 1348
Size: 935 4376
PciI A'CATG_T
Cuts at: 4210 4210
Size: 5311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5311
PmeI GTTT'AAAC
Cuts at: 2615 2615
Size: 5311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 4893 4925 3380
Size: 32 1481 32 3766
Fragments arranged by size:
3766 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5311
PsiI TTA'TAA
Cuts at: 2881 2881
Size: 5311
PspOMI G'GGCC_C
Cuts at: 2584 2584
Size: 5311
PstI C_TGCA'G
Cuts at: 830 2181 2663 830
Size: 1351 482 3478
Fragments arranged by size:
3478 1351 482
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5311
PvuII CAG'CTG
Cuts at: 2128 2182 2128
Size: 54 5257
RsrII CG'GwC_CG
Cuts at: 2101 2101
Size: 5311
SacII CC_GC'GG
Cuts at: 2052 2090 2052
Size: 38 5273
SalI G'TCGA_C
Cuts at: 1936 2653 1936
Size: 717 4594
SbfI CC_TGCA'GG
Cuts at: 2663 2663
Size: 5311
ScaI AGT'ACT
Cuts at: 3559 5271 3559
Size: 1712 3599
SfcI C'TryA_G
Cuts at: 826 1045 2177 2290 2659 3794 4475 4666 826
Size: 219 1132 113 369 1135 681 191 1471
Fragments arranged by size:
1471 1135 1132 681 369 219 191 113
SfoI GGC'GCC
Cuts at: 2056 2075 2138 2569 2056
Size: 19 63 431 4798
Fragments arranged by size:
4798 431 63 19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
SgrAI Cr'CCGG_yG
Cuts at: 1700 2453 1700
Size: 753 4558
SmaI CCC'GGG
Cuts at: 1872 2638 1872
Size: 766 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3415 4316 4578 820
Size: 197 926 1472 901 262 1553
Fragments arranged by size:
1553 1472 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3102 493
Size: 2609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5311
SphI G_CATG'C
Cuts at: 1132 2669 1132
Size: 1537 3774
SspI AAT'ATT
Cuts at: 5 52 3235 5
Size: 47 3183 2081
Fragments arranged by size:
3183 2081 47
StuI AGG'CCT
Cuts at: 1517 2480 1517
Size: 963 4348
StyI C'CwwG_G
Cuts at: 513 1065 2750 513
Size: 552 1685 3074
Fragments arranged by size:
3074 1685 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3338 3355 3508 3693 4138 325
Size: 1255 1758 17 153 185 445 1498
Fragments arranged by size:
1758 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3557 5269 96
Size: 274 80 33 51 1443 1580 1712 138
Fragments arranged by size:
1712 1580 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5271 5303 5271
Size: 32 5279
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5311
XbaI T'CTAG_A
Cuts at: 2647 2647
Size: 5311
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2578 2578
Size: 5311
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5311
XmaI C'CCGG_G
Cuts at: 1870 2636 1870
Size: 766 4545
XmnI GAAnn'nnTTC
Cuts at: 3440 3440
Size: 5311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3119 276
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI SacI SanDI SapI SexAI
SfiI SrfI SwaI