Restriction map for
pFN21AB5246
(Circular) (Six-base) MAPSORT of: pFN21AB5246.seq Check: 9529 from: 1 to: 6145
pFN21AB5246 6145 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3303 3357 3955 278
Size: 53 83 186 936 1767 54 598 2468
Fragments arranged by size:
2468 1767 936 598 186 83 54 53
AccI GT'mk_AC
Cuts at: 1937 3488 1937
Size: 1551 4594
Acc65I G'GTAC_C
Cuts at: 3466 3466
Size: 6145
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2720 3218 4208 5592 5860 1632
Size: 1088 498 990 1384 268 1917
Fragments arranged by size:
1917 1384 1088 990 498 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5948
AflIII A'CryG_T
Cuts at: 1203 2184 5044 6021 1203
Size: 981 2860 977 1327
Fragments arranged by size:
2860 1327 981 977
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 4874 4874
Size: 6145
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6145
AlwNI CAG_nnn'CTG
Cuts at: 3278 3888 5460 3278
Size: 610 1572 3963
Fragments arranged by size:
3963 1572 610
ApaI G_GGCC'C
Cuts at: 2179 2249 2179
Size: 70 6075
ApaLI G'TGCA_C
Cuts at: 4202 5358 4202
Size: 1156 4989
ApoI r'AATT_y
Cuts at: 1493 3454 3680 3832 5941 1493
Size: 1961 226 152 2109 1697
Fragments arranged by size:
2109 1961 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2211 2211
Size: 6145
AseI AT'TA_AT
Cuts at: 160 1032 4699 160
Size: 872 3667 1606
Fragments arranged by size:
3667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 2079 2178 2216 2986 3470 1870
Size: 73 136 99 38 770 484 4545
Fragments arranged by size:
4545 770 484 136 99 73 38
BamHI G'GATC_C
Cuts at: 3475 3842 3475
Size: 367 5778
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2095 2112 2222 2275
Size: 325 217 627 269 39 17 110 53
Cuts at: 2275 2287 2599 2708 3207 3466 4921 618
Size: 12 312 109 499 259 1455 1842
Fragments arranged by size:
1842 1455 627 499 325 312 269 259
217 110 109 53 39 17 12
BanII G_rGCy'C
Cuts at: 1391 2179 2249 2931 3464 1391
Size: 788 70 682 533 4072
Fragments arranged by size:
4072 788 682 533 70
BbeI G_GCGC'C
Cuts at: 2060 2099 2226 2060
Size: 39 127 5979
Fragments arranged by size:
5979 127 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4336 4370 1903
Size: 34 50 34 2315 34 3678
Fragments arranged by size:
3678 2315 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3167 4037 5247 3167
Size: 870 1210 4065
Fragments arranged by size:
4065 1210 870
BclI T'GATC_A
Cuts at: 1548 3414 1548
Size: 1866 4279
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2253 4756 136
Size: 107 122 71 631 605 581 2503 1525
Fragments arranged by size:
2503 1525 631 605 581 122 107 71
BglII A'GATC_T
Cuts at: 6140 6140
Size: 6145
BlpI GC'TnA_GC
Cuts at: 3562 3562
Size: 6145
Bme1580I G_kGCm'C
Cuts at: 1790 2117 2179 2249 2280 4206 5362 1790
Size: 327 62 70 31 1926 1156 2573
Fragments arranged by size:
2573 1926 1156 327 70 62 31
BmgBI CAC'GTC
Cuts at: 1318 2527 3177 3226 1318
Size: 1209 650 49 4237
Fragments arranged by size:
4237 1209 650 49
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3437 3876 4834
Size: 416 53 83 399 24 2020 439 958
Cuts at: 4834 442
Size: 1753
Fragments arranged by size:
2020 1753 958 439 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4805 1239
Size: 3566 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3272 4270 5135 5433 3272
Size: 998 865 298 3984
Fragments arranged by size:
3984 998 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3290 3337 4808 882
Size: 617 1791 47 1471 2219
Fragments arranged by size:
2219 1791 1471 617 47
BsaAI yAC'GTr
Cuts at: 493 1204 2665 3936 493
Size: 711 1461 1271 2702
Fragments arranged by size:
2702 1461 1271 711
BsaBI GATnn'nnATC
Cuts at: 6139 6139
Size: 6145
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2057 2096 2223 2370
Size: 53 83 186 936 524 39 127 147
Cuts at: 2370 2964 3300 3354 3952 4334 4969 275
Size: 594 336 54 598 382 635 1451
Fragments arranged by size:
1451 936 635 598 594 524 382 336
186 147 127 83 54 53 39
BsaWI w'CCGG_w
Cuts at: 4578 4976 5250 5397 5808 4578
Size: 398 274 147 411 4915
Fragments arranged by size:
4915 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2234 2264 2823 2853 1178
Size: 30 165 30 831 30 559 30 4470
Fragments arranged by size:
4470 831 559 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2803 1186
Size: 1617 4528
BseYI C'CCAG_C
Cuts at: 2904 2923 3268 5348 5844 2904
Size: 19 345 2080 496 3205
Fragments arranged by size:
3205 2080 496 345 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2414 1163
Size: 573 678 4894
Fragments arranged by size:
4894 678 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2089 2173 2438 2828 3595 4356
Size: 275 186 88 84 265 390 767 761
Cuts at: 4356 5384 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 767 761 390 275 265 186
88 84
BsiHKAI G_wGCw'C
Cuts at: 2160 2900 4206 4291 5362 2160
Size: 740 1306 85 1071 2943
Fragments arranged by size:
2943 1306 1071 740 85
BsmI GAATG_Cn'
Cuts at: 3656 3749 3656
Size: 93 6052
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2160 2179 2249 2280 2900 2931
Size: 399 327 43 19 70 31 620 31
Cuts at: 2931 3464 4206 4291 5362 1391
Size: 533 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 620 533 399 327 85
70 43 31 31 19
BspEI T'CCGG_A
Cuts at: 5808 5808
Size: 6145
BspHI T'CATG_A
Cuts at: 3411 4034 5764 3411
Size: 623 1730 3792
Fragments arranged by size:
3792 1730 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3500 844
Size: 2656 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2024 2091 2418 2811 4032 1394
Size: 429 201 67 327 393 1221 3507
Fragments arranged by size:
3507 1221 429 393 327 201 67
BsrDI GCAATG_nn'
Cuts at: 65 1248 4640 4814 65
Size: 1183 3392 174 1396
Fragments arranged by size:
3392 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2084 2131 2324 2353 2433 2440 2602
Size: 110 274 47 193 29 80 7 162
Cuts at: 2602 3204 4789 4976 1700
Size: 602 1585 187 2869
Fragments arranged by size:
2869 1585 602 274 193 187 162 110
80 47 29 7
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6145
BssHII G'CGCG_C
Cuts at: 1925 2017 2135 2211 2314 2316 2401 1925
Size: 92 118 76 103 2 85 5669
Fragments arranged by size:
5669 118 103 92 85 76 2
BssSI C'ACGA_G
Cuts at: 4205 5217 4205
Size: 1012 5133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6145
BstBI TT'CG_AA
Cuts at: 4949 4949
Size: 6145
BstEII G'GTnAC_C
Cuts at: 1184 2668 1184
Size: 1484 4661
BstYI r'GATC_y
Cuts at: 1972 2010 2485 3475 3842 4227 4244 5696 5782
Size: 38 475 990 367 385 17 1452 86
Cuts at: 5782 5794 6140 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 990 475 385 367 346 86
38 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2301 2550 3327 513
Size: 552 1236 249 777 3331
Fragments arranged by size:
3331 1236 777 552 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2740 487
Size: 683 56 1514 3892
Fragments arranged by size:
3892 1514 683 56
BtsI GCAGTG_nn'
Cuts at: 2158 2273 3661 4455 4475 2158
Size: 115 1388 794 20 3828
Fragments arranged by size:
3828 1388 794 115 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6110
DraI TTT'AAA
Cuts at: 3449 3449
Size: 6145
DraIII CAC_nnn'GTG
Cuts at: 2668 2668
Size: 6145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5152 809
Size: 507 3836 1802
Fragments arranged by size:
3836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2086 2103 2170
Size: 54 1425 50 275 18 256 17 67
Cuts at: 2170 2298 2435 2470 2806 2825 3592 4481 6110
Size: 128 137 35 336 19 767 889 1629
Cuts at: 6110 8
Size: 43
Fragments arranged by size:
1629 1425 889 767 336 275 256 137
128 67 54 50 43 35 19 18
17
EagI C'GGCC_G
Cuts at: 1537 1812 2086 2170 2435 2825 3592 1537
Size: 275 274 84 265 390 767 4090
Fragments arranged by size:
4090 767 390 275 274 265 84
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 4075 1353
Size: 678 2044 3423
Fragments arranged by size:
3423 2044 678
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3325 4732 5106 5252 387
Size: 1064 138 1736 1407 374 146 1280
Fragments arranged by size:
1736 1407 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2720 3218 4208 4805 5592 5860 1239
Size: 393 1088 498 990 597 787 268 1524
Fragments arranged by size:
1524 1088 990 787 597 498 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2240 2240
Size: 6145
EcoO109I rG'GnC_Cy
Cuts at: 2245 2245
Size: 6145
EcoRI G'AATT_C
Cuts at: 3454 3832 3454
Size: 378 5767
EcoRV GAT'ATC
Cuts at: 3409 3409
Size: 6145
FseI GG_CCGG'CC
Cuts at: 2088 2328 2088
Size: 240 5905
FspI TGC'GCA
Cuts at: 4651 4651
Size: 6145
HaeII r_GCGC'y
Cuts at: 1678 2060 2099 2226 5292 1678
Size: 382 39 127 3066 2531
Fragments arranged by size:
3066 2531 382 127 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3215 3247 4786 4818 4860
Size: 32 135 32 1643 32 1539 32 42
Cuts at: 4860 4892 1373
Size: 32 2626
Fragments arranged by size:
2626 1643 1539 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3489 3735 669
Size: 988 281 1551 246 3079
Fragments arranged by size:
3079 1551 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2576 748
Size: 1828 4317
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KasI G'GCGC_C
Cuts at: 2056 2095 2222 2056
Size: 39 127 5979
Fragments arranged by size:
5979 127 39
KpnI G_GTAC'C
Cuts at: 3470 3470
Size: 6145
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 2184 6021 2184
Size: 3837 2308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2613 5259 5443 1305
Size: 1308 2646 184 2007
Fragments arranged by size:
2646 2007 1308 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4323
Fragments arranged by size:
4323 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4103 4462 4621 518
Size: 648 2937 359 159 2042
Fragments arranged by size:
2937 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2074 2124 2398 3050 3561 4238 5386
Size: 429 39 50 274 652 511 677 1148
Cuts at: 5386 5631 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 652 511 429 274 245
50 39
NaeI GCC'GGC
Cuts at: 2086 2133 2326 2086
Size: 47 193 5905
Fragments arranged by size:
5905 193 47
NarI GG'CG_CC
Cuts at: 2057 2096 2223 2057
Size: 39 127 5979
Fragments arranged by size:
5979 127 39
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5593
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6145
NgoMIV G'CCGG_C
Cuts at: 2084 2131 2324 2084
Size: 47 193 5905
Fragments arranged by size:
5905 193 47
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 3592 3592
Size: 6145
NspI r_CATG'y
Cuts at: 1132 1229 3503 5048 1132
Size: 97 2274 1545 2229
Fragments arranged by size:
2274 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6145
PciI A'CATG_T
Cuts at: 5044 5044
Size: 6145
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6145
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PmlI CAC'GTG
Cuts at: 2665 2665
Size: 6145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 5727 5759 4214
Size: 32 1481 32 4600
Fragments arranged by size:
4600 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6145
PsiI TTA'TAA
Cuts at: 3715 3715
Size: 6145
PspOMI G'GGCC_C
Cuts at: 2175 2245 2175
Size: 70 6075
PstI C_TGCA'G
Cuts at: 830 2241 2268 2457 3497 830
Size: 1411 27 189 1040 3478
Fragments arranged by size:
3478 1411 1040 189 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
PvuII CAG'CTG
Cuts at: 2074 2398 3050 2074
Size: 324 652 5169
Fragments arranged by size:
5169 652 324
SalI G'TCGA_C
Cuts at: 1936 3487 1936
Size: 1551 4594
SapI GCTCTTCn'nnn_
Cuts at: 2031 2031
Size: 6145
SbfI CC_TGCA'GG
Cuts at: 2241 3497 2241
Size: 1256 4889
ScaI AGT'ACT
Cuts at: 4393 6105 4393
Size: 1712 4433
SfcI C'TryA_G
Cuts at: 826 1045 2237 2264 2453 3493 4628 5309 5500
Size: 219 1192 27 189 1040 1135 681 191
Cuts at: 5500 826
Size: 1471
Fragments arranged by size:
1471 1192 1135 1040 681 219 191 189
27
SfoI GGC'GCC
Cuts at: 2058 2097 2224 2058
Size: 39 127 5979
Fragments arranged by size:
5979 127 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 2081 2180 3472 1872
Size: 209 99 1292 4545
Fragments arranged by size:
4545 1292 209 99
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3287 4249 5150 5412 820
Size: 197 926 1344 962 901 262 1553
Fragments arranged by size:
1553 1344 962 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 3503 1132
Size: 2371 3774
SspI AAT'ATT
Cuts at: 5 52 4069 5
Size: 47 4017 2081
Fragments arranged by size:
4017 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6145
StyI C'CwwG_G
Cuts at: 513 1065 2473 3584 513
Size: 552 1408 1111 3074
Fragments arranged by size:
3074 1408 1111 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2918 2975 3126 4172 4189 4342 4527
Size: 1255 1338 57 151 1046 17 153 185
Cuts at: 4527 4972 325
Size: 445 1498
Fragments arranged by size:
1498 1338 1255 1046 445 185 153 151
57 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4391 6103 96
Size: 274 80 33 51 1443 2414 1712 138
Fragments arranged by size:
2414 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2234 2266 2634 2666 2823 2855 3312 3344 3409
Size: 32 368 32 157 32 457 32 65
Cuts at: 3409 3441 6105 6137 2234
Size: 32 2664 32 2242
Fragments arranged by size:
2664 2242 457 368 157 65 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 3481 3481
Size: 6145
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6145
XmaI C'CCGG_G
Cuts at: 1870 2079 2178 3470 1870
Size: 209 99 1292 4545
Fragments arranged by size:
4545 1292 209 99
XmnI GAAnn'nnTTC
Cuts at: 2568 3255 4274 2568
Size: 687 1019 4439
Fragments arranged by size:
4439 1019 687
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3301 3355 3953 276
Size: 53 83 186 936 1767 54 598 2468
Fragments arranged by size:
2468 1767 936 598 186 83 54 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI
FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI
XcmI