Restriction map for
pFN21AB5244
(Circular) (Six-base) MAPSORT of: pFN21AB5244.seq Check: 5688 from: 1 to: 5773
pFN21AB5244 5773 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2631 2631
Size: 5773
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3583 278
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3116 1937
Size: 1179 4594
Acc65I G'GTAC_C
Cuts at: 2699 3094 2699
Size: 395 5378
AclI AA'CG_TT
Cuts at: 1563 3900 4273 1563
Size: 2337 373 3063
Fragments arranged by size:
3063 2337 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3836 5220 5488 1632
Size: 2204 1384 268 1917
Fragments arranged by size:
2204 1917 1384 268
AfeI AGC'GCT
Cuts at: 2638 2760 2638
Size: 122 5651
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5576
AflIII A'CryG_T
Cuts at: 1203 4672 5649 1203
Size: 3469 977 1327
Fragments arranged by size:
3469 1327 977
AgeI A'CCGG_T
Cuts at: 4604 4604
Size: 5773
AhdI GACnn_n'nnGTC
Cuts at: 4502 4502
Size: 5773
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2402 2931 1184
Size: 1218 529 4026
Fragments arranged by size:
4026 1218 529
AlwNI CAG_nnn'CTG
Cuts at: 2413 3516 5088 2413
Size: 1103 1572 3098
Fragments arranged by size:
3098 1572 1103
ApaI G_GGCC'C
Cuts at: 2938 2938
Size: 5773
ApaLI G'TGCA_C
Cuts at: 3830 4986 3830
Size: 1156 4617
ApoI r'AATT_y
Cuts at: 1493 3082 3308 3460 5569 1493
Size: 1589 226 152 2109 1697
Fragments arranged by size:
2109 1697 1589 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4327 160
Size: 872 3295 1606
Fragments arranged by size:
3295 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
AvaI C'yCGr_G
Cuts at: 1870 1943 2328 3098 1870
Size: 73 385 770 4545
Fragments arranged by size:
4545 770 385 73
BamHI G'GATC_C
Cuts at: 3103 3470 3103
Size: 367 5406
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2386 2699 2963 3094 4549
Size: 325 217 627 599 313 264 131 1455
Cuts at: 4549 618
Size: 1842
Fragments arranged by size:
1842 1455 627 599 325 313 264 217
131
BanII G_rGCy'C
Cuts at: 1391 2461 2769 2938 3092 1391
Size: 1070 308 169 154 4072
Fragments arranged by size:
4072 1070 308 169 154
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2713 928
Size: 971 814 3988
Fragments arranged by size:
3988 971 814
BbvCI CC'TCA_GC
Cuts at: 2046 2822 2046
Size: 776 4997
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3964 3998 1903
Size: 34 2027 34 3678
Fragments arranged by size:
3678 2027 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3665 4875 3665
Size: 1210 4563
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5773
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4384 136
Size: 107 122 71 631 605 2712 1525
Fragments arranged by size:
2712 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5768 5768
Size: 5773
BlpI GC'TnA_GC
Cuts at: 2834 3190 2834
Size: 356 5417
Bme1580I G_kGCm'C
Cuts at: 1790 2938 3049 3834 4990 1790
Size: 1148 111 785 1156 2573
Fragments arranged by size:
2573 1156 1148 785 111
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5773
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2679 3504 4462
Size: 416 53 83 399 24 1262 825 958
Cuts at: 4462 442
Size: 1753
Fragments arranged by size:
1753 1262 958 825 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5773
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 5773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 2783 4433 1239
Size: 1073 471 1650 2579
Fragments arranged by size:
2579 1650 1073 471
Bpu10I CC'TnA_GC
Cuts at: 2046 2479 2595 2822 2046
Size: 433 116 227 4997
Fragments arranged by size:
4997 433 227 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3898 4763 5061 3898
Size: 865 298 4610
Fragments arranged by size:
4610 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2506 4436 882
Size: 617 1007 1930 2219
Fragments arranged by size:
2219 1930 1007 617
BsaAI yAC'GTr
Cuts at: 493 1204 3564 493
Size: 711 2360 2702
Fragments arranged by size:
2702 2360 711
BsaBI GATnn'nnATC
Cuts at: 2235 5767 2235
Size: 3532 2241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3580 3962 4597 275
Size: 53 83 186 936 2047 382 635 1451
Fragments arranged by size:
2047 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4206 4604 4878 5025 5436 4206
Size: 398 274 147 411 4543
Fragments arranged by size:
4543 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2486 2516 2645 2675 1178
Size: 30 165 30 1083 30 129 30 4276
Fragments arranged by size:
4276 1083 165 129 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2165 2192 2195 2222 2240 1186
Size: 928 51 27 3 27 18 4719
Fragments arranged by size:
4719 928 51 27 27 18 3
BseYI C'CCAG_C
Cuts at: 3048 4976 5472 3048
Size: 1928 496 3349
Fragments arranged by size:
3349 1928 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5200
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3223 3984 5012 1540
Size: 275 186 1222 761 1028 2301
Fragments arranged by size:
2301 1222 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 3834 3919 4990 2106
Size: 1728 85 1071 2889
Fragments arranged by size:
2889 1728 1071 85
BsmI GAATG_Cn'
Cuts at: 3284 3377 3284
Size: 93 5680
BsmBI CGTCTCn'nnnn_
Cuts at: 2245 2302 2245
Size: 57 5716
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2461 2769 2938 3049 3092 3834
Size: 399 316 355 308 169 111 43 742
Cuts at: 3834 3919 4990 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 355 316 308 169
111 85 43
BspEI T'CCGG_A
Cuts at: 5436 5436
Size: 5773
BspHI T'CATG_A
Cuts at: 3028 3039 3067 3662 5392 3028
Size: 11 28 595 1730 3409
Fragments arranged by size:
3409 1730 595 28 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2382 2631 3128 844
Size: 1538 249 497 3489
Fragments arranged by size:
3489 1538 497 249
BsrBI CCG'CTC
Cuts at: 1394 1823 2335 2833 3660 1394
Size: 429 512 498 827 3507
Fragments arranged by size:
3507 827 512 498 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4268 4442 65
Size: 1183 3020 174 1396
Fragments arranged by size:
3020 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4417 4604 1700
Size: 110 2607 187 2869
Fragments arranged by size:
2869 2607 187 110
BsrGI T'GTAC_A
Cuts at: 96 2650 96
Size: 2554 3219
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5773
BssSI C'ACGA_G
Cuts at: 2105 3833 4845 2105
Size: 1728 1012 3033
Fragments arranged by size:
3033 1728 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5773
BstBI TT'CG_AA
Cuts at: 4577 4577
Size: 5773
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5773
BstXI CCAn_nnnn'nTGG
Cuts at: 2551 2675 2551
Size: 124 5649
BstYI r'GATC_y
Cuts at: 1972 2469 2985 3103 3470 3855 3872 5324 5410
Size: 497 516 118 367 385 17 1452 86
Cuts at: 5410 5422 5768 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 516 497 385 367 346 118
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2703 2944 513
Size: 552 1638 241 3342
Fragments arranged by size:
3342 1638 552 241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5034
Fragments arranged by size:
5034 683 56
BtsI GCAGTG_nn'
Cuts at: 3289 4083 4103 3289
Size: 794 20 4959
Fragments arranged by size:
4959 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5738
DraI TTT'AAA
Cuts at: 2302 3077 2302
Size: 775 4998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4780 809
Size: 507 3464 1802
Fragments arranged by size:
3464 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2439 2631 3220
Size: 54 1425 50 275 18 609 192 589
Cuts at: 3220 4109 5738 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 609 589 275 192 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3220 1537
Size: 275 1408 4090
Fragments arranged by size:
4090 1408 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 2211 2217 3703 1353
Size: 846 12 6 1486 3423
Fragments arranged by size:
3423 1486 846 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2679 2727 2863 4360 4734 4880
Size: 1064 138 1090 48 136 1497 374 146
Cuts at: 4880 387
Size: 1280
Fragments arranged by size:
1497 1280 1090 1064 374 146 138 136
48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2783 3836 4433 5220 5488 1239
Size: 393 680 471 1053 597 787 268 1524
Fragments arranged by size:
1524 1053 787 680 597 471 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2204 2378 2204
Size: 174 5599
EcoO109I rG'GnC_Cy
Cuts at: 2057 2197 2934 2057
Size: 140 737 4896
Fragments arranged by size:
4896 737 140
EcoRI G'AATT_C
Cuts at: 3082 3460 3082
Size: 378 5395
FspI TGC'GCA
Cuts at: 2378 4279 2378
Size: 1901 3872
HaeII r_GCGC'y
Cuts at: 1678 2366 2640 2762 4920 1678
Size: 688 274 122 2158 2531
Fragments arranged by size:
2531 2158 688 274 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2269 2301 2486 2518 4414
Size: 32 135 32 697 32 185 32 1896
Cuts at: 4414 4446 4488 4520 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1896 697 185 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3117 3363 669
Size: 988 281 1179 246 3079
Fragments arranged by size:
3079 1179 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5773
HpaI GTT'AAC
Cuts at: 3363 3363
Size: 5773
KpnI G_GTAC'C
Cuts at: 2703 3098 2703
Size: 395 5378
MfeI C'AATT_G
Cuts at: 3372 3372
Size: 5773
MluI A'CGCG_T
Cuts at: 5649 5649
Size: 5773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2445 4887 5071 1305
Size: 1140 2442 184 2007
Fragments arranged by size:
2442 2007 1140 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2441 10
Size: 54 1425 343 609 3342
Fragments arranged by size:
3342 1425 609 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3731 4090 4249 518
Size: 648 2565 359 159 2042
Fragments arranged by size:
2565 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2969 3189 3866 5014 5259 1606
Size: 1363 220 677 1148 245 2120
Fragments arranged by size:
2120 1363 1148 677 245 220
NcoI C'CATG_G
Cuts at: 513 1065 2703 513
Size: 552 1638 3583
Fragments arranged by size:
3583 1638 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5773
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5773
NotI GC'GGCC_GC
Cuts at: 3220 3220
Size: 5773
NspI r_CATG'y
Cuts at: 1132 1229 2347 2614 3131 4676 1132
Size: 97 1118 267 517 1545 2229
Fragments arranged by size:
2229 1545 1118 517 267 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5773
PciI A'CATG_T
Cuts at: 4672 4672
Size: 5773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5773
PmeI GTTT'AAAC
Cuts at: 3077 3077
Size: 5773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 5355 5387 3842
Size: 32 1481 32 4228
Fragments arranged by size:
4228 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2057 2197 2057
Size: 140 5633
PshAI GACnn'nnGTC
Cuts at: 1613 2079 1613
Size: 466 5307
PsiI TTA'TAA
Cuts at: 3343 3343
Size: 5773
PspOMI G'GGCC_C
Cuts at: 2934 2934
Size: 5773
PstI C_TGCA'G
Cuts at: 830 2920 3125 830
Size: 2090 205 3478
Fragments arranged by size:
3478 2090 205
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5773
SalI G'TCGA_C
Cuts at: 1936 3115 1936
Size: 1179 4594
SbfI CC_TGCA'GG
Cuts at: 3125 3125
Size: 5773
ScaI AGT'ACT
Cuts at: 4021 5733 4021
Size: 1712 4061
SexAI A'CCwGG_T
Cuts at: 2496 2550 2496
Size: 54 5719
SfcI C'TryA_G
Cuts at: 826 1045 2749 2916 3121 4256 4937 5128 826
Size: 219 1704 167 205 1135 681 191 1471
Fragments arranged by size:
1704 1471 1135 681 219 205 191 167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5773
SmaI CCC'GGG
Cuts at: 1872 2330 3100 1872
Size: 458 770 4545
Fragments arranged by size:
4545 770 458
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3877 4778 5040 820
Size: 197 926 1934 901 262 1553
Fragments arranged by size:
1934 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3564 493
Size: 3071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5773
SphI G_CATG'C
Cuts at: 1132 2347 3131 1132
Size: 1215 784 3774
Fragments arranged by size:
3774 1215 784
SspI AAT'ATT
Cuts at: 5 52 3001 3697 5
Size: 47 2949 696 2081
Fragments arranged by size:
2949 2081 696 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5773
StyI C'CwwG_G
Cuts at: 513 1065 2053 2703 3212 513
Size: 552 988 650 509 3074
Fragments arranged by size:
3074 988 650 552 509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3800 3817 3970 4155 4600 325
Size: 1255 2220 17 153 185 445 1498
Fragments arranged by size:
2220 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2650 4019 5731
Size: 274 80 33 51 1443 673 1369 1712
Cuts at: 5731 96
Size: 138
Fragments arranged by size:
1712 1443 1369 673 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2645 2677 2855 2887 5733 5765 2645
Size: 32 178 32 2846 32 2653
Fragments arranged by size:
2846 2653 178 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2498 1316
Size: 1182 4591
XbaI T'CTAG_A
Cuts at: 3109 3109
Size: 5773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2548 2548
Size: 5773
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5773
XmaI C'CCGG_G
Cuts at: 1870 2328 3098 1870
Size: 458 770 4545
Fragments arranged by size:
4545 770 458
XmnI GAAnn'nnTTC
Cuts at: 3902 3902
Size: 5773
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3581 276
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI