Restriction map for
pFN21AB5241
(Circular) (Six-base) MAPSORT of: pFN21AB5241.seq Check: 9430 from: 1 to: 6139
pFN21AB5241 6139 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3192 3949 278
Size: 53 83 186 936 1656 757 2468
Fragments arranged by size:
2468 1656 936 757 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3342 3482 1937
Size: 1405 140 4594
Fragments arranged by size:
4594 1405 140
Acc65I G'GTAC_C
Cuts at: 3460 3460
Size: 6139
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 2474 2669 2699 2727 3102 4202 5586
Size: 608 234 195 30 28 375 1100 1384
Cuts at: 5586 5854 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1100 608 375 268 234 195
30 28
AfeI AGC'GCT
Cuts at: 2539 2539
Size: 6139
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5942
AflIII A'CryG_T
Cuts at: 1203 2742 5038 6015 1203
Size: 1539 2296 977 1327
Fragments arranged by size:
2296 1539 1327 977
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 4868 4868
Size: 6139
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6139
AlwNI CAG_nnn'CTG
Cuts at: 3882 5454 3882
Size: 1572 4567
ApaI G_GGCC'C
Cuts at: 3394 3394
Size: 6139
ApaLI G'TGCA_C
Cuts at: 4196 5352 4196
Size: 1156 4983
ApoI r'AATT_y
Cuts at: 1493 2135 3088 3170 3448 3674 3826 5935 1493
Size: 642 953 82 278 226 152 2109 1697
Fragments arranged by size:
2109 1697 953 642 278 226 152 82
AseI AT'TA_AT
Cuts at: 160 1032 4693 160
Size: 872 3661 1606
Fragments arranged by size:
3661 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 3464 1870
Size: 73 1521 4545
Fragments arranged by size:
4545 1521 73
BamHI G'GATC_C
Cuts at: 3469 3836 3469
Size: 367 5772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3460 4915 618
Size: 325 217 627 1673 1455 1842
Fragments arranged by size:
1842 1673 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3394 3458 1391
Size: 2003 64 4072
Fragments arranged by size:
4072 2003 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4330 4364 1903
Size: 34 2393 34 3678
Fragments arranged by size:
3678 2393 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4031 5241 4031
Size: 1210 4929
BclI T'GATC_A
Cuts at: 1548 2023 1548
Size: 475 5664
BfrBI ATG'CAT
Cuts at: 3108 3108
Size: 6139
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2571 2938 4750
Size: 107 122 71 631 605 899 367 1812
Cuts at: 4750 136
Size: 1525
Fragments arranged by size:
1812 1525 899 631 605 367 122 107
71
BglII A'GATC_T
Cuts at: 3310 6134 3310
Size: 2824 3315
BlpI GC'TnA_GC
Cuts at: 3556 3556
Size: 6139
Bme1580I G_kGCm'C
Cuts at: 1790 3394 4200 5356 1790
Size: 1604 806 1156 2573
Fragments arranged by size:
2573 1604 1156 806
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3870 4828 442
Size: 416 53 83 399 24 2453 958 1753
Fragments arranged by size:
2453 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 3395 4799 1239
Size: 827 1329 1404 2579
Fragments arranged by size:
2579 1404 1329 827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2837 3343 4264 5129 5427 2837
Size: 506 921 865 298 3549
Fragments arranged by size:
3549 921 865 506 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4802 882
Size: 617 3303 2219
Fragments arranged by size:
3303 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3930 493
Size: 711 2726 2702
Fragments arranged by size:
2726 2702 711
BsaBI GATnn'nnATC
Cuts at: 2025 2906 6133 2025
Size: 881 3227 2031
Fragments arranged by size:
3227 2031 881
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3189 3946 4328 4963
Size: 53 83 186 936 1656 757 382 635
Cuts at: 4963 275
Size: 1451
Fragments arranged by size:
1656 1451 936 757 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4572 4970 5244 5391 5802 4572
Size: 398 274 147 411 4909
Fragments arranged by size:
4909 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5914
Fragments arranged by size:
5914 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2296 1186
Size: 1110 5029
BseYI C'CCAG_C
Cuts at: 2540 5342 5838 2540
Size: 2802 496 2841
Fragments arranged by size:
2841 2802 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5566
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3589 4350 5378 1540
Size: 275 186 1588 761 1028 2301
Fragments arranged by size:
2301 1588 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4200 4285 5356 4200
Size: 85 1071 4983
Fragments arranged by size:
4983 1071 85
BsmI GAATG_Cn'
Cuts at: 3650 3743 3650
Size: 93 6046
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3394 3458 4200 4285 5356 1391
Size: 399 1604 64 742 85 1071 2174
Fragments arranged by size:
2174 1604 1071 742 399 85 64
BspEI T'CCGG_A
Cuts at: 5802 5802
Size: 6139
BspHI T'CATG_A
Cuts at: 4028 5758 4028
Size: 1730 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3494 844
Size: 2650 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3388 4026 1394
Size: 429 1565 638 3507
Fragments arranged by size:
3507 1565 638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4634 4808 65
Size: 1183 3386 174 1396
Fragments arranged by size:
3386 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4783 4970 1700
Size: 110 2973 187 2869
Fragments arranged by size:
2973 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6139
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6139
BssSI C'ACGA_G
Cuts at: 4199 5211 4199
Size: 1012 5127
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6139
BstBI TT'CG_AA
Cuts at: 4943 4943
Size: 6139
BstEII G'GTnAC_C
Cuts at: 1184 2290 1184
Size: 1106 5033
BstYI r'GATC_y
Cuts at: 1972 3310 3469 3836 4221 4238 5690 5776 5788
Size: 1338 159 367 385 17 1452 86 12
Cuts at: 5788 6134 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1338 385 367 346 159 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2621 2621
Size: 6139
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5587
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5400
Fragments arranged by size:
5400 683 56
BtsI GCAGTG_nn'
Cuts at: 3006 3655 4449 4469 3006
Size: 649 794 20 4676
Fragments arranged by size:
4676 794 649 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6104
DraI TTT'AAA
Cuts at: 2578 3213 3443 2578
Size: 635 230 5274
Fragments arranged by size:
5274 635 230
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3586 4475 6104
Size: 54 1425 50 275 18 1756 889 1629
Cuts at: 6104 8
Size: 43
Fragments arranged by size:
1756 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3586 1537
Size: 275 1774 4090
Fragments arranged by size:
4090 1774 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 3078 4069 1353
Size: 863 862 991 3423
Fragments arranged by size:
3423 991 863 862
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4726 5100 5246 387
Size: 1064 138 3137 374 146 1280
Fragments arranged by size:
3137 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2240 2474 2669 2699 2727 3102
Size: 393 434 174 234 195 30 28 375
Cuts at: 3102 3395 4202 4799 5586 5854 1239
Size: 293 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 597 434 393 375 293
268 234 195 174 30 28
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2624 2360
Size: 264 5875
EcoO109I rG'GnC_Cy
Cuts at: 3391 3434 3391
Size: 43 6096
EcoRI G'AATT_C
Cuts at: 2135 3448 3826 2135
Size: 1313 378 4448
Fragments arranged by size:
4448 1313 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 6139
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 2541 5286 1678
Size: 863 2745 2531
Fragments arranged by size:
2745 2531 863
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3418 3450 4780 4812 4854
Size: 32 135 32 1846 32 1330 32 42
Cuts at: 4854 4886 1373
Size: 32 2626
Fragments arranged by size:
2626 1846 1330 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3483 3729 669
Size: 988 281 1545 246 3079
Fragments arranged by size:
3079 1545 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2384 2681 3319 748
Size: 1636 297 638 3568
Fragments arranged by size:
3568 1636 638 297
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KpnI G_GTAC'C
Cuts at: 3464 3464
Size: 6139
MfeI C'AATT_G
Cuts at: 2051 3738 2051
Size: 1687 4452
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5253 5437 1305
Size: 3948 184 2007
Fragments arranged by size:
3948 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4317
Fragments arranged by size:
4317 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4097 4456 4615 518
Size: 648 2931 359 159 2042
Fragments arranged by size:
2931 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3555 4232 5380 5625 1606
Size: 1949 677 1148 245 2120
Fragments arranged by size:
2120 1949 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5587
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6139
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6139
NotI GC'GGCC_GC
Cuts at: 3586 3586
Size: 6139
NsiI A_TGCA'T
Cuts at: 3110 3110
Size: 6139
NspI r_CATG'y
Cuts at: 1132 1229 2746 3497 5042 1132
Size: 97 1517 751 1545 2229
Fragments arranged by size:
2229 1545 1517 751 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6139
PciI A'CATG_T
Cuts at: 2742 5038 2742
Size: 2296 3843
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2651 1108
Size: 1543 4596
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 5721 5753 4208
Size: 32 1481 32 4594
Fragments arranged by size:
4594 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3434 3434
Size: 6139
PshAI GACnn'nnGTC
Cuts at: 1613 2759 1613
Size: 1146 4993
PsiI TTA'TAA
Cuts at: 3709 3709
Size: 6139
PspOMI G'GGCC_C
Cuts at: 3390 3390
Size: 6139
PstI C_TGCA'G
Cuts at: 830 3001 3491 830
Size: 2171 490 3478
Fragments arranged by size:
3478 2171 490
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
SalI G'TCGA_C
Cuts at: 1936 3481 1936
Size: 1545 4594
SapI GCTCTTCn'nnn_
Cuts at: 2216 2216
Size: 6139
SbfI CC_TGCA'GG
Cuts at: 3491 3491
Size: 6139
ScaI AGT'ACT
Cuts at: 3380 4387 6099 3380
Size: 1007 1712 3420
Fragments arranged by size:
3420 1712 1007
SfcI C'TryA_G
Cuts at: 826 1045 2404 2997 3487 4622 5303 5494 826
Size: 219 1359 593 490 1135 681 191 1471
Fragments arranged by size:
1471 1359 1135 681 593 490 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6139
SmaI CCC'GGG
Cuts at: 1872 3466 1872
Size: 1594 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2816 3322 4243 5144 5406 820
Size: 197 926 873 506 921 901 262 1553
Fragments arranged by size:
1553 926 921 901 873 506 262 197
SnaBI TAC'GTA
Cuts at: 493 3930 493
Size: 3437 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6139
SphI G_CATG'C
Cuts at: 1132 3497 1132
Size: 2365 3774
SspI AAT'ATT
Cuts at: 5 52 4063 5
Size: 47 4011 2081
Fragments arranged by size:
4011 2081 47
StuI AGG'CCT
Cuts at: 1517 2489 2834 1517
Size: 972 345 4822
Fragments arranged by size:
4822 972 345
StyI C'CwwG_G
Cuts at: 513 1065 2484 3331 3578 513
Size: 552 1419 847 247 3074
Fragments arranged by size:
3074 1419 847 552 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2863 4166 4183 4336 4521 4966 325
Size: 1255 1283 1303 17 153 185 445 1498
Fragments arranged by size:
1498 1303 1283 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2009 2718 3378
Size: 274 80 33 51 1443 32 709 660
Cuts at: 3378 4385 6097 96
Size: 1007 1712 138
Fragments arranged by size:
1712 1443 1007 709 660 274 138 80
51 33 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2886 2918 6099 6131 2886
Size: 32 3181 32 2894
Fragments arranged by size:
3181 2894 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3432 1316
Size: 2116 4023
XbaI T'CTAG_A
Cuts at: 3034 3475 3034
Size: 441 5698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2155 3022 2155
Size: 867 5272
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6139
XmaI C'CCGG_G
Cuts at: 1870 3464 1870
Size: 1594 4545
XmnI GAAnn'nnTTC
Cuts at: 4268 4268
Size: 6139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3190 3947 276
Size: 53 83 186 936 1656 757 2468
Fragments arranged by size:
2468 1656 936 757 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI