Restriction map for
pFN21AB5235
(Circular) (Six-base) MAPSORT of: pFN21AB5235.seq Check: 2097 from: 1 to: 6232
pFN21AB5235 6232 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2718 3575 1937
Size: 781 857 4594
Fragments arranged by size:
4594 857 781
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2027 2147 2375 2594 2600 2930 4295 5679
Size: 395 120 228 219 6 330 1365 1384
Cuts at: 5679 5947 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1365 395 330 268 228 219
120 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6035
AflIII A'CryG_T
Cuts at: 1203 5131 6108 1203
Size: 3928 977 1327
Fragments arranged by size:
3928 1327 977
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 2734 4961 2734
Size: 2227 4005
AleI CACnn'nnGTG
Cuts at: 1166 3002 1166
Size: 1836 4396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6232
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2838 2870 2838
Size: 32 6200
AlwNI CAG_nnn'CTG
Cuts at: 3975 5547 3975
Size: 1572 4660
ApaLI G'TGCA_C
Cuts at: 4289 5445 4289
Size: 1156 5076
ApoI r'AATT_y
Cuts at: 1493 2162 2513 3541 3767 3919 6028 1493
Size: 669 351 1028 226 152 2109 1697
Fragments arranged by size:
2109 1697 1028 669 351 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4786 160
Size: 872 3754 1606
Fragments arranged by size:
3754 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 2979 3557 1870
Size: 73 1036 578 4545
Fragments arranged by size:
4545 1036 578 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2274 2307 2274
Size: 33 6199
BamHI G'GATC_C
Cuts at: 2860 3562 3929 2860
Size: 702 367 5163
Fragments arranged by size:
5163 702 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3133 3553 5008 618
Size: 325 217 627 1346 420 1455 1842
Fragments arranged by size:
1842 1455 1346 627 420 325 217
BanII G_rGCy'C
Cuts at: 1391 2376 3057 3150 3551 1391
Size: 985 681 93 401 4072
Fragments arranged by size:
4072 985 681 401 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2337 3486 928
Size: 971 438 1149 3674
Fragments arranged by size:
3674 1149 971 438
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4423 4457 1903
Size: 34 2486 34 3678
Fragments arranged by size:
3678 2486 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2845 4124 5334 2845
Size: 1279 1210 3743
Fragments arranged by size:
3743 1279 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4843 136
Size: 107 122 71 631 605 3171 1525
Fragments arranged by size:
3171 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6227 6227
Size: 6232
BlpI GC'TnA_GC
Cuts at: 3149 3318 3649 3149
Size: 169 331 5732
Fragments arranged by size:
5732 331 169
Bme1580I G_kGCm'C
Cuts at: 1790 2189 4293 5449 1790
Size: 399 2104 1156 2573
Fragments arranged by size:
2573 2104 1156 399
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3009 3963 4921
Size: 416 53 83 399 24 1592 954 958
Cuts at: 4921 442
Size: 1753
Fragments arranged by size:
1753 1592 958 954 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2994 2994
Size: 6232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2840 3102 4892 1239
Size: 806 795 262 1790 2579
Fragments arranged by size:
2579 1790 806 795 262
Bpu10I CC'TnA_GC
Cuts at: 2969 2969
Size: 6232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 3401 4357 5222 5520 2051
Size: 1350 956 865 298 2763
Fragments arranged by size:
2763 1350 956 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2898 4895 882
Size: 617 1399 1997 2219
Fragments arranged by size:
2219 1997 1399 617
BsaAI yAC'GTr
Cuts at: 493 1204 4023 493
Size: 711 2819 2702
Fragments arranged by size:
2819 2702 711
BsaBI GATnn'nnATC
Cuts at: 6226 6226
Size: 6232
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4039 4421 5056 275
Size: 53 83 186 936 2506 382 635 1451
Fragments arranged by size:
2506 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4665 5063 5337 5484 5895 4665
Size: 398 274 147 411 5002
Fragments arranged by size:
5002 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2040 2070 2363 2393 2838
Size: 30 165 30 637 30 293 30 445
Cuts at: 2838 2868 2942 2972 1178
Size: 30 74 30 4438
Fragments arranged by size:
4438 637 445 293 165 74 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2114 2117 2701 3221 3386 1186
Size: 856 72 3 584 520 165 4032
Fragments arranged by size:
4032 856 584 520 165 72 3
BseYI C'CCAG_C
Cuts at: 2137 2485 2540 3034 3305 5435 5931 2137
Size: 348 55 494 271 2130 496 2438
Fragments arranged by size:
2438 2130 496 494 348 271 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2289 3234 1163
Size: 573 553 945 4161
Fragments arranged by size:
4161 945 573 553
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2644 3682 4443 5471 1540
Size: 275 186 643 1038 761 1028 2301
Fragments arranged by size:
2301 1038 1028 761 643 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 2376 4293 4378 5449 2172
Size: 204 1917 85 1071 2955
Fragments arranged by size:
2955 1917 1071 204 85
BsmI GAATG_Cn'
Cuts at: 2649 3743 3836 2649
Size: 1094 93 5045
Fragments arranged by size:
5045 1094 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2189 2376 3057 3150 3551 4293
Size: 399 382 17 187 681 93 401 742
Cuts at: 4293 4378 5449 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 681 401 399 382 187
93 85 17
BspEI T'CCGG_A
Cuts at: 5895 5895
Size: 6232
BspHI T'CATG_A
Cuts at: 4121 5851 4121
Size: 1730 4502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3587 844
Size: 2743 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3166 4119 1394
Size: 429 1343 953 3507
Fragments arranged by size:
3507 1343 953 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2430 2535 4727 4901 65
Size: 1183 1182 105 2192 174 1396
Fragments arranged by size:
2192 1396 1183 1182 174 105
BsrFI r'CCGG_y
Cuts at: 1700 1810 4876 5063 1700
Size: 110 3066 187 2869
Fragments arranged by size:
3066 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3327 96
Size: 3231 3001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 4292 5304 4292
Size: 1012 5220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2015 794
Size: 1221 5011
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6232
BstXI CCAn_nnnn'nTGG
Cuts at: 2825 2825
Size: 6232
BstYI r'GATC_y
Cuts at: 1972 2595 2860 3562 3929 4314 4331 5783 5869
Size: 623 265 702 367 385 17 1452 86
Cuts at: 5869 5881 6227 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 702 623 385 367 346 265
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2787 2787
Size: 6232
BtgI C'CryG_G
Cuts at: 513 1065 2004 2263 2497 3222 513
Size: 552 939 259 234 725 3523
Fragments arranged by size:
3523 939 725 552 259 234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5493
Fragments arranged by size:
5493 683 56
BtsI GCAGTG_nn'
Cuts at: 3024 3076 3748 4542 4562 3024
Size: 52 672 794 20 4694
Fragments arranged by size:
4694 794 672 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6197
DraI TTT'AAA
Cuts at: 3536 3536
Size: 6232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2627 5239 809
Size: 507 1311 2612 1802
Fragments arranged by size:
2612 1802 1311 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2500 3679 4568
Size: 54 1425 50 275 18 670 1179 889
Cuts at: 4568 6197 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1179 889 670 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2739 3045 3453 3469 4162 1353
Size: 1386 306 408 16 693 3423
Fragments arranged by size:
3423 1386 693 408 306 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4819 5193 5339 387
Size: 1064 138 3230 374 146 1280
Fragments arranged by size:
3230 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2374 2374
Size: 6232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2027 2045 2147 2375 2594 2600 2840
Size: 393 395 18 102 228 219 6 240
Cuts at: 2840 2930 3102 4295 4892 5679 5947 1239
Size: 90 172 1193 597 787 268 1524
Fragments arranged by size:
1524 1193 787 597 395 393 268 240
228 219 172 102 90 18 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2630 3488 2630
Size: 858 5374
EcoO109I rG'GnC_Cy
Cuts at: 2141 2776 2896 2141
Size: 635 120 5477
Fragments arranged by size:
5477 635 120
EcoRI G'AATT_C
Cuts at: 2513 3541 3919 2513
Size: 1028 378 4826
Fragments arranged by size:
4826 1028 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2905 2935 2905
Size: 30 6202
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 2024 2088 2263 5379 1678
Size: 346 64 175 3116 2531
Fragments arranged by size:
3116 2531 346 175 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 2720 2752 2942
Size: 32 135 32 791 32 325 32 190
Cuts at: 2942 2974 4873 4905 4947 4979 1373
Size: 32 1899 32 42 32 2626
Fragments arranged by size:
2626 1899 791 325 190 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3576 3822 669
Size: 988 281 1638 246 3079
Fragments arranged by size:
3079 1638 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2339 3281 748
Size: 1591 942 3699
Fragments arranged by size:
3699 1591 942
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5346 5530 1305
Size: 4041 184 2007
Fragments arranged by size:
4041 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4410
Fragments arranged by size:
4410 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3002 3158 4190 4549 4708 518
Size: 648 1836 156 1032 359 159 2042
Fragments arranged by size:
2042 1836 1032 648 359 159 156
MspA1I CmG'CkG
Cuts at: 1606 2137 2851 3648 4325 5473 5718 1606
Size: 531 714 797 677 1148 245 2120
Fragments arranged by size:
2120 1148 797 714 677 531 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4741
Fragments arranged by size:
4741 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6232
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6232
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NruI TCG'CGA
Cuts at: 2223 2223
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 3112 3590 5135 1132
Size: 97 1883 478 1545 2229
Fragments arranged by size:
2229 1883 1545 478 97
PasI CC'CwG_GG
Cuts at: 1348 3058 1348
Size: 1710 4522
PciI A'CATG_T
Cuts at: 5131 5131
Size: 6232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6232
PfoI T'CCnGG_A
Cuts at: 2856 2856
Size: 6232
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2838 2870 4301 4333 5814 5846 2838
Size: 32 1431 32 1481 32 3224
Fragments arranged by size:
3224 1481 1431 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2141 2141
Size: 6232
PshAI GACnn'nnGTC
Cuts at: 1613 2199 1613
Size: 586 5646
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PspXI vC'TCGA_Gb
Cuts at: 2979 2979
Size: 6232
PstI C_TGCA'G
Cuts at: 830 3584 830
Size: 2754 3478
PvuI CG_AT'CG
Cuts at: 2001 2644 2001
Size: 643 5589
PvuII CAG'CTG
Cuts at: 2137 2851 2137
Size: 714 5518
SacI G_AGCT'C
Cuts at: 2376 2376
Size: 6232
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SapI GCTCTTCn'nnn_
Cuts at: 3045 3045
Size: 6232
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 3365 4480 6192 3365
Size: 1115 1712 3405
Fragments arranged by size:
3405 1712 1115
SfcI C'TryA_G
Cuts at: 826 1045 2197 3026 3580 4715 5396 5587 826
Size: 219 1152 829 554 1135 681 191 1471
Fragments arranged by size:
1471 1152 1135 829 681 554 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 3559 1872
Size: 1687 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2066 2979 3416 4336 5237 5499
Size: 197 926 123 913 437 920 901 262
Cuts at: 5499 820
Size: 1553
Fragments arranged by size:
1553 926 920 913 901 437 262 197
123
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3590 1132
Size: 2458 3774
SspI AAT'ATT
Cuts at: 5 52 4156 5
Size: 47 4104 2081
Fragments arranged by size:
4104 2081 47
StuI AGG'CCT
Cuts at: 1517 2064 2888 1517
Size: 547 824 4861
Fragments arranged by size:
4861 824 547
StyI C'CwwG_G
Cuts at: 513 1065 2004 2181 2889 3671 513
Size: 552 939 177 708 782 3074
Fragments arranged by size:
3074 939 782 708 552 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3050 4259 4276 4429 4614 5059 325
Size: 1255 1470 1209 17 153 185 445 1498
Fragments arranged by size:
1498 1470 1255 1209 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3327 3363 4478
Size: 274 80 33 51 1443 1350 36 1115
Cuts at: 4478 6190 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1350 1115 274 138 80 51
36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2040 2072 6192 6224 2040
Size: 32 4120 32 2048
Fragments arranged by size:
4120 2048 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2813 1316
Size: 1497 4735
XbaI T'CTAG_A
Cuts at: 2653 3568 2653
Size: 915 5317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2825 2825
Size: 6232
XhoI C'TCGA_G
Cuts at: 1943 2979 1943
Size: 1036 5196
XmaI C'CCGG_G
Cuts at: 1870 3557 1870
Size: 1687 4545
XmnI GAAnn'nnTTC
Cuts at: 4361 4361
Size: 6232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NsiI PacI PmlI PspOMI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI