Restriction map for
pFN21AB5229
(Circular) (Six-base) MAPSORT of: pFN21AB5229.seq Check: 9390 from: 1 to: 5584
pFN21AB5229 5584 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3394 278
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2927 1937
Size: 990 4594
Acc65I G'GTAC_C
Cuts at: 2905 2905
Size: 5584
AclI AA'CG_TT
Cuts at: 1563 3711 4084 1563
Size: 2148 373 3063
Fragments arranged by size:
3063 2148 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2901 3647 5031 5299 1632
Size: 1269 746 1384 268 1917
Fragments arranged by size:
1917 1384 1269 746 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5387
AflIII A'CryG_T
Cuts at: 1203 4483 5460 1203
Size: 3280 977 1327
Fragments arranged by size:
3280 1327 977
AgeI A'CCGG_T
Cuts at: 4415 4415
Size: 5584
AhdI GACnn_n'nnGTC
Cuts at: 4313 4313
Size: 5584
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5584
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5584
AlwNI CAG_nnn'CTG
Cuts at: 3327 4899 3327
Size: 1572 4012
ApaLI G'TGCA_C
Cuts at: 3641 4797 3641
Size: 1156 4428
ApoI r'AATT_y
Cuts at: 1493 2250 2814 2893 3119 3271 5380 1493
Size: 757 564 79 226 152 2109 1697
Fragments arranged by size:
2109 1697 757 564 226 152 79
AseI AT'TA_AT
Cuts at: 160 1032 4138 160
Size: 872 3106 1606
Fragments arranged by size:
3106 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
AvaI C'yCGr_G
Cuts at: 1870 1943 2909 1870
Size: 73 966 4545
Fragments arranged by size:
4545 966 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2308 2341 2308
Size: 33 5551
BamHI G'GATC_C
Cuts at: 2162 2914 3281 2162
Size: 752 367 4465
Fragments arranged by size:
4465 752 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2905 4360 618
Size: 325 217 627 1118 1455 1842
Fragments arranged by size:
1842 1455 1118 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2903 1391
Size: 1512 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4613
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3775 3809 1903
Size: 34 1838 34 3678
Fragments arranged by size:
3678 1838 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2336 3476 4686 2336
Size: 1140 1210 3234
Fragments arranged by size:
3234 1210 1140
BclI T'GATC_A
Cuts at: 1548 2626 1548
Size: 1078 4506
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4195 136
Size: 107 122 71 631 605 2523 1525
Fragments arranged by size:
2523 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5579 5579
Size: 5584
BlpI GC'TnA_GC
Cuts at: 3001 3001
Size: 5584
Bme1580I G_kGCm'C
Cuts at: 1790 3645 4801 1790
Size: 1855 1156 2573
Fragments arranged by size:
2573 1855 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5584
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3315 4273 442
Size: 416 53 83 399 24 1898 958 1753
Fragments arranged by size:
1898 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4244 1239
Size: 3005 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3709 4574 4872 3709
Size: 865 298 4421
Fragments arranged by size:
4421 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4247 882
Size: 617 2748 2219
Fragments arranged by size:
2748 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3375 493
Size: 711 2171 2702
Fragments arranged by size:
2702 2171 711
BsaBI GATnn'nnATC
Cuts at: 5578 5578
Size: 5584
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3391 3773 4408 275
Size: 53 83 186 936 1858 382 635 1451
Fragments arranged by size:
1858 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4017 4415 4689 4836 5247 4017
Size: 398 274 147 411 4354
Fragments arranged by size:
4354 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5359
Fragments arranged by size:
5359 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5584
BseYI C'CCAG_C
Cuts at: 4787 5283 4787
Size: 496 5088
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2460 1163
Size: 573 724 4287
Fragments arranged by size:
4287 724 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3034 3795 4823 1540
Size: 275 186 1033 761 1028 2301
Fragments arranged by size:
2301 1033 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3645 3730 4801 3645
Size: 85 1071 4428
Fragments arranged by size:
4428 1071 85
BsmI GAATG_Cn'
Cuts at: 2403 3095 3188 2403
Size: 692 93 4799
Fragments arranged by size:
4799 692 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2903 3645 3730 4801 1391
Size: 399 1113 742 85 1071 2174
Fragments arranged by size:
2174 1113 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5247 5247
Size: 5584
BspHI T'CATG_A
Cuts at: 2176 2524 2752 3473 5203 2176
Size: 348 228 721 1730 2557
Fragments arranged by size:
2557 1730 721 348 228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2939 844
Size: 2095 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3471 1394
Size: 429 1648 3507
Fragments arranged by size:
3507 1648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4079 4253 65
Size: 1183 2831 174 1396
Fragments arranged by size:
2831 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4228 4415 1700
Size: 110 2418 187 2869
Fragments arranged by size:
2869 2418 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5584
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5584
BssSI C'ACGA_G
Cuts at: 3644 4656 3644
Size: 1012 4572
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5584
BstBI TT'CG_AA
Cuts at: 4388 4388
Size: 5584
BstEII G'GTnAC_C
Cuts at: 1184 2653 1184
Size: 1469 4115
BstXI CCAn_nnnn'nTGG
Cuts at: 2274 2274
Size: 5584
BstYI r'GATC_y
Cuts at: 1972 2162 2914 3281 3666 3683 5135 5221 5233
Size: 190 752 367 385 17 1452 86 12
Cuts at: 5233 5579 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 752 385 367 346 190 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4093
Fragments arranged by size:
4093 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4845
Fragments arranged by size:
4845 683 56
BtsI GCAGTG_nn'
Cuts at: 3100 3894 3914 3100
Size: 794 20 4770
Fragments arranged by size:
4770 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5584
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5549
DraI TTT'AAA
Cuts at: 2104 2718 2888 2104
Size: 614 170 4800
Fragments arranged by size:
4800 614 170
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4591 809
Size: 507 3275 1802
Fragments arranged by size:
3275 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3031 3920 5549
Size: 54 1425 50 275 18 1201 889 1629
Cuts at: 5549 8
Size: 43
Fragments arranged by size:
1629 1425 1201 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3031 1537
Size: 275 1219 4090
Fragments arranged by size:
4090 1219 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3514 1353
Size: 2161 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4171 4545 4691 387
Size: 1064 138 2582 374 146 1280
Fragments arranged by size:
2582 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2901 3647 4244 5031 5299 1239
Size: 393 1269 746 597 787 268 1524
Fragments arranged by size:
1524 1269 787 746 597 393 268
EcoRI G'AATT_C
Cuts at: 2893 3271 2893
Size: 378 5206
FspI TGC'GCA
Cuts at: 4090 4090
Size: 5584
HaeII r_GCGC'y
Cuts at: 1678 4731 1678
Size: 3053 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4225 4257 4299 4331 1373
Size: 32 135 32 2653 32 42 32 2626
Fragments arranged by size:
2653 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2928 3174 669
Size: 988 281 990 246 3079
Fragments arranged by size:
3079 990 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5584
HpaI GTT'AAC
Cuts at: 3174 3174
Size: 5584
KpnI G_GTAC'C
Cuts at: 2909 2909
Size: 5584
MfeI C'AATT_G
Cuts at: 3183 3183
Size: 5584
MluI A'CGCG_T
Cuts at: 5460 5460
Size: 5584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4698 4882 1305
Size: 3393 184 2007
Fragments arranged by size:
3393 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3762
Fragments arranged by size:
3762 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2800 3542 3901 4060 518
Size: 648 1634 742 359 159 2042
Fragments arranged by size:
2042 1634 742 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2332 3000 3677 4825 5070 1606
Size: 726 668 677 1148 245 2120
Fragments arranged by size:
2120 1148 726 677 668 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4093
Fragments arranged by size:
4093 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5584
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5584
NotI GC'GGCC_GC
Cuts at: 3031 3031
Size: 5584
NspI r_CATG'y
Cuts at: 1132 1229 2942 4487 1132
Size: 97 1713 1545 2229
Fragments arranged by size:
2229 1713 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5584
PciI A'CATG_T
Cuts at: 4483 4483
Size: 5584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5584
PmeI GTTT'AAAC
Cuts at: 2888 2888
Size: 5584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2815 2847 3653 3685 5166 5198 2815
Size: 32 806 32 1481 32 3201
Fragments arranged by size:
3201 1481 806 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5584
PsiI TTA'TAA
Cuts at: 3154 3154
Size: 5584
PstI C_TGCA'G
Cuts at: 830 2936 830
Size: 2106 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5584
PvuII CAG'CTG
Cuts at: 2332 2332
Size: 5584
SalI G'TCGA_C
Cuts at: 1936 2926 1936
Size: 990 4594
SbfI CC_TGCA'GG
Cuts at: 2936 2936
Size: 5584
ScaI AGT'ACT
Cuts at: 3832 5544 3832
Size: 1712 3872
SfcI C'TryA_G
Cuts at: 826 1045 2932 4067 4748 4939 826
Size: 219 1887 1135 681 191 1471
Fragments arranged by size:
1887 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5584
SmaI CCC'GGG
Cuts at: 1872 2911 1872
Size: 1039 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3688 4589 4851 820
Size: 197 926 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3375 493
Size: 2882 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5584
SphI G_CATG'C
Cuts at: 1132 2942 1132
Size: 1810 3774
SspI AAT'ATT
Cuts at: 5 52 2377 3508 5
Size: 47 2325 1131 2081
Fragments arranged by size:
2325 2081 1131 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5584
StyI C'CwwG_G
Cuts at: 513 1065 2004 2046 3023 513
Size: 552 939 42 977 3074
Fragments arranged by size:
3074 977 939 552 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2641 3611 3628 3781 3966 4411 325
Size: 1255 1061 970 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1061 970 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3830 5542 96
Size: 274 80 33 51 1443 1853 1712 138
Fragments arranged by size:
1853 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5544 5576 5544
Size: 32 5552
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5584
XbaI T'CTAG_A
Cuts at: 2920 2920
Size: 5584
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5584
XmaI C'CCGG_G
Cuts at: 1870 2909 1870
Size: 1039 4545
XmnI GAAnn'nnTTC
Cuts at: 2294 3713 2294
Size: 1419 4165
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3392 276
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI