Restriction map for
pFN21AB5227
(Circular) (Six-base) MAPSORT of: pFN21AB5227.seq Check: 8072 from: 1 to: 6187
pFN21AB5227 6187 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2770 2770
Size: 6187
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2138 3035 3530 1937
Size: 201 897 495 4594
Fragments arranged by size:
4594 897 495 201
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 4314 4687 1563
Size: 2751 373 3063
Fragments arranged by size:
3063 2751 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2051 4250 5634 5902 1632
Size: 419 2199 1384 268 1917
Fragments arranged by size:
2199 1917 1384 419 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5990
AflIII A'CryG_T
Cuts at: 1203 5086 6063 1203
Size: 3883 977 1327
Fragments arranged by size:
3883 1327 977
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 4916 4916
Size: 6187
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6187
AlwNI CAG_nnn'CTG
Cuts at: 2147 2777 3930 5502 2147
Size: 630 1153 1572 2832
Fragments arranged by size:
2832 1572 1153 630
ApaLI G'TGCA_C
Cuts at: 4244 5400 4244
Size: 1156 5031
ApoI r'AATT_y
Cuts at: 1493 2703 3496 3722 3874 5983 1493
Size: 1210 793 226 152 2109 1697
Fragments arranged by size:
2109 1697 1210 793 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2492 4741 160
Size: 872 1460 2249 1606
Fragments arranged by size:
2249 1606 1460 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 3512 1870
Size: 73 1569 4545
Fragments arranged by size:
4545 1569 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3179 3212 3179
Size: 33 6154
BamHI G'GATC_C
Cuts at: 3517 3884 3517
Size: 367 5820
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3409 3508 4963 618
Size: 325 217 627 1622 99 1455 1842
Fragments arranged by size:
1842 1622 1455 627 325 217 99
BanII G_rGCy'C
Cuts at: 1391 3506 1391
Size: 2115 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5216
BbvCI CC'TCA_GC
Cuts at: 2406 2406
Size: 6187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4378 4412 1903
Size: 34 2441 34 3678
Fragments arranged by size:
3678 2441 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3356 4079 5289 3356
Size: 723 1210 4254
Fragments arranged by size:
4254 1210 723
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4798 136
Size: 107 122 71 631 605 3126 1525
Fragments arranged by size:
3126 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6182 6182
Size: 6187
BlpI GC'TnA_GC
Cuts at: 2152 3604 2152
Size: 1452 4735
Bme1580I G_kGCm'C
Cuts at: 1790 3184 3414 4248 5404 1790
Size: 1394 230 834 1156 2573
Fragments arranged by size:
2573 1394 1156 834 230
BmgBI CAC'GTC
Cuts at: 1318 2696 1318
Size: 1378 4809
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2575 2814 3918
Size: 416 53 83 399 24 1158 239 1104
Cuts at: 3918 4876 442
Size: 958 1753
Fragments arranged by size:
1753 1158 1104 958 416 399 239 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2460 2781 4847 1239
Size: 1221 321 2066 2579
Fragments arranged by size:
2579 2066 1221 321
Bpu10I CC'TnA_GC
Cuts at: 2406 2928 2406
Size: 522 5665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2703 2839 4312 5177 5475 2703
Size: 136 1473 865 298 3415
Fragments arranged by size:
3415 1473 865 298 136
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3301 3448 4850 882
Size: 617 1802 147 1402 2219
Fragments arranged by size:
2219 1802 1402 617 147
BsaAI yAC'GTr
Cuts at: 493 1204 3978 493
Size: 711 2774 2702
Fragments arranged by size:
2774 2702 711
BsaBI GATnn'nnATC
Cuts at: 6181 6181
Size: 6187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3994 4376 5011 275
Size: 53 83 186 936 2461 382 635 1451
Fragments arranged by size:
2461 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4620 5018 5292 5439 5850 4620
Size: 398 274 147 411 4957
Fragments arranged by size:
4957 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5962
Fragments arranged by size:
5962 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2499 3423 1186
Size: 1313 924 3950
Fragments arranged by size:
3950 1313 924
BseYI C'CCAG_C
Cuts at: 5390 5886 5390
Size: 496 5691
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2748 1163
Size: 573 1012 4602
Fragments arranged by size:
4602 1012 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3637 4398 5426 1540
Size: 275 186 1636 761 1028 2301
Fragments arranged by size:
2301 1636 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2464 4248 4333 5404 2464
Size: 1784 85 1071 3247
Fragments arranged by size:
3247 1784 1071 85
BsmI GAATG_Cn'
Cuts at: 2475 2529 2670 3698 3791 2475
Size: 54 141 1028 93 4871
Fragments arranged by size:
4871 1028 141 93 54
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2464 3184 3414 3506 4248 4333 5404
Size: 399 674 720 230 92 742 85 1071
Cuts at: 5404 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 720 674 399 230 92
85
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 4076 5806 4076
Size: 1730 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2161 2770 2791 3542 844
Size: 1317 609 21 751 3489
Fragments arranged by size:
3489 1317 751 609 21
BsrBI CCG'CTC
Cuts at: 1394 1823 4074 1394
Size: 429 2251 3507
Fragments arranged by size:
3507 2251 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2161 4682 4856 65
Size: 1183 913 2521 174 1396
Fragments arranged by size:
2521 1396 1183 913 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4831 5018 1700
Size: 110 3021 187 2869
Fragments arranged by size:
3021 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6187
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6187
BssSI C'ACGA_G
Cuts at: 4247 5259 4247
Size: 1012 5175
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6187
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstYI r'GATC_y
Cuts at: 1972 2076 3517 3884 4269 4286 5738 5824 5836
Size: 104 1441 367 385 17 1452 86 12
Cuts at: 5836 6182 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1441 385 367 346 104 86
17 12
BstZ17I GTA'TAC
Cuts at: 2139 2139
Size: 6187
BtgI C'CryG_G
Cuts at: 513 1065 2004 2036 513
Size: 552 939 32 4664
Fragments arranged by size:
4664 939 552 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5448
Fragments arranged by size:
5448 683 56
BtsI GCAGTG_nn'
Cuts at: 3703 4497 4517 3703
Size: 794 20 5373
Fragments arranged by size:
5373 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2538 2573 529
Size: 35 1974 35 4143
Fragments arranged by size:
4143 1974 35 35
DraI TTT'AAA
Cuts at: 2110 3491 2110
Size: 1381 4806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5194 809
Size: 507 3878 1802
Fragments arranged by size:
3878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3215 3478 3634
Size: 54 1425 50 275 18 1385 263 156
Cuts at: 3634 4523 6152 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1385 889 275 263 156 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3634 1537
Size: 275 1822 4090
Fragments arranged by size:
4090 1822 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3165 4117 1353
Size: 1812 952 3423
Fragments arranged by size:
3423 1812 952
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4774 5148 5294 387
Size: 1064 138 3185 374 146 1280
Fragments arranged by size:
3185 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2460 2781 4250 4847 5634 5902
Size: 393 419 409 321 1469 597 787 268
Cuts at: 5902 1239
Size: 1524
Fragments arranged by size:
1524 1469 787 597 419 409 393 321
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2409 2637 3216 2409
Size: 228 579 5380
Fragments arranged by size:
5380 579 228
EcoO109I rG'GnC_Cy
Cuts at: 2144 2945 3226 2144
Size: 801 281 5105
Fragments arranged by size:
5105 801 281
EcoRI G'AATT_C
Cuts at: 2703 3496 3874 2703
Size: 793 378 5016
Fragments arranged by size:
5016 793 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2880 2880
Size: 6187
FspI TGC'GCA
Cuts at: 4693 4693
Size: 6187
HaeII r_GCGC'y
Cuts at: 1678 2741 5334 1678
Size: 1063 2593 2531
Fragments arranged by size:
2593 2531 1063
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 3146 3178 4828
Size: 32 135 32 776 32 766 32 1650
Cuts at: 4828 4860 4902 4934 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1650 776 766 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3531 3777 669
Size: 988 281 1593 246 3079
Fragments arranged by size:
3079 1593 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6187
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3255 3786 3255
Size: 531 5656
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2214 3229 5301 5485 1305
Size: 909 1015 2072 184 2007
Fragments arranged by size:
2072 2007 1015 909 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4365
Fragments arranged by size:
4365 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2606 3150 4145 4504 4663 518
Size: 648 1440 544 995 359 159 2042
Fragments arranged by size:
2042 1440 995 648 544 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2936 3083 3603 4280 5428 5673 1606
Size: 423 907 147 520 677 1148 245 2120
Fragments arranged by size:
2120 1148 907 677 520 423 245 147
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4696
Fragments arranged by size:
4696 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6187
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 3634 3634
Size: 6187
NspI r_CATG'y
Cuts at: 1132 1229 2170 2548 3358 3545 5090 1132
Size: 97 941 378 810 187 1545 2229
Fragments arranged by size:
2229 1545 941 810 378 187 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6187
PciI A'CATG_T
Cuts at: 5086 5086
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2618 2699 3248 3326 1108
Size: 1510 81 549 78 3969
Fragments arranged by size:
3969 1510 549 81 78
PfoI T'CCnGG_A
Cuts at: 2698 2698
Size: 6187
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 5769 5801 4256
Size: 32 1481 32 4642
Fragments arranged by size:
4642 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2144 2945 2144
Size: 801 5386
PshAI GACnn'nnGTC
Cuts at: 1613 2843 1613
Size: 1230 4957
PsiI TTA'TAA
Cuts at: 3757 3757
Size: 6187
PstI C_TGCA'G
Cuts at: 830 2371 3073 3539 830
Size: 1541 702 466 3478
Fragments arranged by size:
3478 1541 702 466
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
PvuII CAG'CTG
Cuts at: 2029 2936 2029
Size: 907 5280
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 3067 4435 6147 3067
Size: 1368 1712 3107
Fragments arranged by size:
3107 1712 1368
SexAI A'CCwGG_T
Cuts at: 2671 2671
Size: 6187
SfcI C'TryA_G
Cuts at: 826 1045 2367 2994 3036 3069 3535 4670 5351
Size: 219 1322 627 42 33 466 1135 681
Cuts at: 5351 5542 826
Size: 191 1471
Fragments arranged by size:
1471 1322 1135 681 627 466 219 191
42 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 3514 1872
Size: 1642 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2718 2854 4291 5192 5454 820
Size: 197 926 775 136 1437 901 262 1553
Fragments arranged by size:
1553 1437 926 901 775 262 197 136
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 3050 3122 152
Size: 2898 72 3217
Fragments arranged by size:
3217 2898 72
SphI G_CATG'C
Cuts at: 1132 2170 2548 3358 3545 1132
Size: 1038 378 810 187 3774
Fragments arranged by size:
3774 1038 810 378 187
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6187
StyI C'CwwG_G
Cuts at: 513 1065 2004 2941 3626 513
Size: 552 939 937 685 3074
Fragments arranged by size:
3074 939 937 685 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2594 2606 4214 4231 4384 4569 5014
Size: 1255 1014 12 1608 17 153 185 445
Cuts at: 5014 325
Size: 1498
Fragments arranged by size:
1608 1498 1255 1014 445 185 153 17
12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2858 3053 3065
Size: 274 80 33 51 1443 881 195 12
Cuts at: 3065 4433 6145 96
Size: 1368 1712 138
Fragments arranged by size:
1712 1443 1368 881 274 195 138 80
51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6147 6179 6147
Size: 32 6155
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6187
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2948 2948
Size: 6187
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 3512 1870
Size: 1642 4545
XmnI GAAnn'nnTTC
Cuts at: 2256 4316 2256
Size: 2060 4127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI