Restriction map for
pFN21AB5225
(Circular) (Six-base) MAPSORT of: pFN21AB5225.seq Check: 8371 from: 1 to: 6808
pFN21AB5225 6808 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2856 3029 2856
Size: 173 6635
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2373 3829 4618 278
Size: 53 83 186 936 837 1456 789 2468
Fragments arranged by size:
2468 1456 936 837 789 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4151 1937
Size: 2214 4594
Acc65I G'GTAC_C
Cuts at: 3204 4129 3204
Size: 925 5883
AclI AA'CG_TT
Cuts at: 1563 4107 4935 5308 1563
Size: 2544 828 373 3063
Fragments arranged by size:
3063 2544 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2597 2618 2984 3146 3567 4871 6255 6523
Size: 965 21 366 162 421 1304 1384 268
Cuts at: 6523 1632
Size: 1917
Fragments arranged by size:
1917 1384 1304 965 421 366 268 162
21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6611
AflIII A'CryG_T
Cuts at: 1203 3432 5707 6684 1203
Size: 2229 2275 977 1327
Fragments arranged by size:
2275 2229 1327 977
AgeI A'CCGG_T
Cuts at: 5639 5639
Size: 6808
AhdI GACnn_n'nnGTC
Cuts at: 5537 5537
Size: 6808
AleI CACnn'nnGTG
Cuts at: 1166 3512 1166
Size: 2346 4462
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6808
AlwNI CAG_nnn'CTG
Cuts at: 2119 3611 4551 6123 2119
Size: 1492 940 1572 2804
Fragments arranged by size:
2804 1572 1492 940
ApaI G_GGCC'C
Cuts at: 3142 3142
Size: 6808
ApaLI G'TGCA_C
Cuts at: 4865 6021 4865
Size: 1156 5652
ApoI r'AATT_y
Cuts at: 1493 2497 4117 4343 4495 6604 1493
Size: 1004 1620 226 152 2109 1697
Fragments arranged by size:
2109 1697 1620 1004 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5362 160
Size: 872 4330 1606
Fragments arranged by size:
4330 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6808
AvaI C'yCGr_G
Cuts at: 1870 1943 2530 3182 4133 1870
Size: 73 587 652 951 4545
Fragments arranged by size:
4545 951 652 587 73
AvrII C'CTAG_G
Cuts at: 2518 2518
Size: 6808
BamHI G'GATC_C
Cuts at: 4138 4505 4138
Size: 367 6441
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3041 3204 3616 3709 3900
Size: 325 217 627 1254 163 412 93 191
Cuts at: 3900 4129 5584 618
Size: 229 1455 1842
Fragments arranged by size:
1842 1455 1254 627 412 325 229 217
191 163 93
BanII G_rGCy'C
Cuts at: 1391 2735 3142 3853 4027 4127 1391
Size: 1344 407 711 174 100 4072
Fragments arranged by size:
4072 1344 711 407 174 100
BbeI G_GCGC'C
Cuts at: 3713 3904 3713
Size: 191 6617
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3345 928
Size: 971 1446 4391
Fragments arranged by size:
4391 1446 971
BbvCI CC'TCA_GC
Cuts at: 2759 3191 3888 2759
Size: 432 697 5679
Fragments arranged by size:
5679 697 432
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4999 5033 1903
Size: 34 3062 34 3678
Fragments arranged by size:
3678 3062 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4700 5910 4700
Size: 1210 5598
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6808
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2135 2523 3718
Size: 107 122 71 631 605 463 388 1195
Cuts at: 3718 3898 4031 5419 136
Size: 180 133 1388 1525
Fragments arranged by size:
1525 1388 1195 631 605 463 388 180
133 122 107 71
BglII A'GATC_T
Cuts at: 6803 6803
Size: 6808
BlpI GC'TnA_GC
Cuts at: 2754 4225 2754
Size: 1471 5337
Bme1580I G_kGCm'C
Cuts at: 1790 3046 3142 3519 4869 6025 1790
Size: 1256 96 377 1350 1156 2573
Fragments arranged by size:
2573 1350 1256 1156 377 96
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6808
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3668 3959 4539
Size: 416 53 83 399 24 2251 291 580
Cuts at: 4539 5497 442
Size: 958 1753
Fragments arranged by size:
2251 1753 958 580 416 399 291 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6808
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 6808
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2381 2423 2717 3026 3435 3446 3537
Size: 902 240 42 294 309 409 11 91
Cuts at: 3537 3543 3750 5468 1239
Size: 6 207 1718 2579
Fragments arranged by size:
2579 1718 902 409 309 294 240 207
91 42 11 6
Bpu10I CC'TnA_GC
Cuts at: 2759 3191 3888 2759
Size: 432 697 5679
Fragments arranged by size:
5679 697 432
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2453 2957 3032 4933 5798 6096 2453
Size: 504 75 1901 865 298 3165
Fragments arranged by size:
3165 1901 865 504 298 75
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2066 2936 3748 4094 5471 882
Size: 617 567 870 812 346 1377 2219
Fragments arranged by size:
2219 1377 870 812 617 567 346
BsaAI yAC'GTr
Cuts at: 493 1204 4599 493
Size: 711 3395 2702
Fragments arranged by size:
3395 2702 711
BsaBI GATnn'nnATC
Cuts at: 2715 6802 2715
Size: 4087 2721
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2370 3372 3710 3826
Size: 53 83 186 936 837 1002 338 116
Cuts at: 3826 3901 4615 4997 5632 275
Size: 75 714 382 635 1451
Fragments arranged by size:
1451 1002 936 837 714 635 382 338
186 116 83 75 53
BsaWI w'CCGG_w
Cuts at: 5241 5639 5913 6060 6471 5241
Size: 398 274 147 411 5578
Fragments arranged by size:
5578 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3523 3553 1178
Size: 30 165 30 2120 30 4433
Fragments arranged by size:
4433 2120 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 2417 2597 2600 2603 2606 3023 3251
Size: 1228 3 180 3 3 3 417 228
Cuts at: 3251 3748 4035 1186
Size: 497 287 3959
Fragments arranged by size:
3959 1228 497 417 287 228 180 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2857 2948 3263 3330 3523 3571 3929 3959 4057
Size: 91 315 67 193 48 358 30 98
Cuts at: 4057 6011 6507 2857
Size: 1954 496 3158
Fragments arranged by size:
3158 1954 496 358 315 193 98 91
67 48 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2006 3187 3969 1163
Size: 573 270 1181 782 4002
Fragments arranged by size:
4002 1181 782 573 270
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4258 5019 6047 1540
Size: 275 186 2257 761 1028 2301
Fragments arranged by size:
2301 2257 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2735 4869 4954 6025 2735
Size: 2134 85 1071 3518
Fragments arranged by size:
3518 2134 1071 85
BsmI GAATG_Cn'
Cuts at: 4319 4412 4319
Size: 93 6715
BsmBI CGTCTCn'nnnn_
Cuts at: 2564 2696 2975 2564
Size: 132 279 6397
Fragments arranged by size:
6397 279 132
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2735 3046 3142 3519 3853 4027 4127
Size: 399 945 311 96 377 334 174 100
Cuts at: 4127 4869 4954 6025 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 945 742 399 377 334 311
174 100 96 85
BspEI T'CCGG_A
Cuts at: 6471 6471
Size: 6808
BspHI T'CATG_A
Cuts at: 2895 4697 6427 2895
Size: 1802 1730 3276
Fragments arranged by size:
3276 1802 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2095 2106 2712 2856 3029 4163 844
Size: 1251 11 606 144 173 1134 3489
Fragments arranged by size:
3489 1251 1134 606 173 144 11
BsrBI CCG'CTC
Cuts at: 1394 1823 2187 3756 4695 1394
Size: 429 364 1569 939 3507
Fragments arranged by size:
3507 1569 939 429 364
BsrDI GCAATG_nn'
Cuts at: 65 1248 2621 5303 5477 65
Size: 1183 1373 2682 174 1396
Fragments arranged by size:
2682 1396 1373 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2125 3909 5452 5639 1700
Size: 110 315 1784 1543 187 2869
Fragments arranged by size:
2869 1784 1543 315 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6808
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6808
BssSI C'ACGA_G
Cuts at: 2447 4868 5880 2447
Size: 2421 1012 3375
Fragments arranged by size:
3375 2421 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2029 794
Size: 1235 5573
BstBI TT'CG_AA
Cuts at: 5612 5612
Size: 6808
BstEII G'GTnAC_C
Cuts at: 1184 3643 1184
Size: 2459 4349
BstXI CCAn_nnnn'nTGG
Cuts at: 2245 2566 3283 3364 2245
Size: 321 717 81 5689
Fragments arranged by size:
5689 717 321 81
BstYI r'GATC_y
Cuts at: 1972 4138 4505 4890 4907 6359 6445 6457 6803
Size: 2166 367 385 17 1452 86 12 346
Cuts at: 6803 1972
Size: 1977
Fragments arranged by size:
2166 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3200 4029 3200
Size: 829 5979
BtgI C'CryG_G
Cuts at: 513 1065 2553 2770 513
Size: 552 1488 217 4551
Fragments arranged by size:
4551 1488 552 217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2702 3256 487
Size: 683 56 1476 554 4039
Fragments arranged by size:
4039 1476 683 554 56
BtsI GCAGTG_nn'
Cuts at: 3507 4324 5118 5138 3507
Size: 817 794 20 5177
Fragments arranged by size:
5177 817 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6808
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6773
DraI TTT'AAA
Cuts at: 4112 4112
Size: 6808
DraIII CAC_nnn'GTG
Cuts at: 3034 3034
Size: 6808
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5815 809
Size: 507 4499 1802
Fragments arranged by size:
4499 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2340 2556
Size: 54 1425 50 275 18 297 213 216
Cuts at: 2556 2773 3231 3302 4255 5144 6773 8
Size: 217 458 71 953 889 1629 43
Fragments arranged by size:
1629 1425 953 889 458 297 275 217
216 213 71 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4255 1537
Size: 275 2443 4090
Fragments arranged by size:
4090 2443 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2006 2594 2919 2939 3413 3449 4738 1353
Size: 653 588 325 20 474 36 1289 3423
Fragments arranged by size:
3423 1289 653 588 474 325 36 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5395 5769 5915 387
Size: 1064 138 3806 374 146 1280
Fragments arranged by size:
3806 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2733 2733
Size: 6808
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2381 2423 2597 2618 2717 2984
Size: 393 509 240 42 174 21 99 267
Cuts at: 2984 3026 3146 3435 3446 3537 3543 3567 3750
Size: 42 120 289 11 91 6 24 183
Cuts at: 3750 4871 5468 6255 6523 1239
Size: 1121 597 787 268 1524
Fragments arranged by size:
1524 1121 787 597 509 393 289 268
267 240 183 174 120 99 91 42
42 24 21 11 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2090 2102 3158 2090
Size: 12 1056 5740
Fragments arranged by size:
5740 1056 12
EcoO109I rG'GnC_Cy
Cuts at: 2095 2952 3138 3151 3287 3804 4045 2095
Size: 857 186 13 136 517 241 4858
Fragments arranged by size:
4858 857 517 241 186 136 13
EcoRI G'AATT_C
Cuts at: 2497 4117 4495 2497
Size: 1620 378 4810
Fragments arranged by size:
4810 1620 378
FspI TGC'GCA
Cuts at: 5314 5314
Size: 6808
HaeII r_GCGC'y
Cuts at: 1678 3386 3706 3713 3904 5955 1678
Size: 1708 320 7 191 2051 2531
Fragments arranged by size:
2531 2051 1708 320 191 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3329 3361 5449 5481 5523
Size: 32 135 32 1757 32 2088 32 42
Cuts at: 5523 5555 1373
Size: 32 2626
Fragments arranged by size:
2626 2088 1757 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4152 4398 669
Size: 988 281 2214 246 3079
Fragments arranged by size:
3079 2214 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2216 748
Size: 1468 5340
HpaI GTT'AAC
Cuts at: 4398 4398
Size: 6808
KasI G'GCGC_C
Cuts at: 3709 3900 3709
Size: 191 6617
KpnI G_GTAC'C
Cuts at: 3208 4133 3208
Size: 925 5883
MfeI C'AATT_G
Cuts at: 4407 4407
Size: 6808
MluI A'CGCG_T
Cuts at: 6684 6684
Size: 6808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2478 3045 5922 6106 1305
Size: 1173 567 2877 184 2007
Fragments arranged by size:
2877 2007 1173 567 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2558 3304 10
Size: 54 1425 343 726 746 3514
Fragments arranged by size:
3514 1425 746 726 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 3362 3512 4766 5125 5284 518
Size: 648 1077 1119 150 1254 359 159 2042
Fragments arranged by size:
2042 1254 1119 1077 648 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 2020 2038 2078 2116 2326 3334 3452 3668
Size: 414 18 40 38 210 1008 118 216
Cuts at: 3668 4224 4901 6049 6294 1606
Size: 556 677 1148 245 2120
Fragments arranged by size:
2120 1148 1008 677 556 414 245 216
210 118 40 38 18
NaeI GCC'GGC
Cuts at: 2127 3911 2127
Size: 1784 5024
NarI GG'CG_CC
Cuts at: 3710 3901 3710
Size: 191 6617
NcoI C'CATG_G
Cuts at: 513 1065 2553 513
Size: 552 1488 4768
Fragments arranged by size:
4768 1488 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6808
NgoMIV G'CCGG_C
Cuts at: 2125 3909 2125
Size: 1784 5024
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6808
NotI GC'GGCC_GC
Cuts at: 4255 4255
Size: 6808
NspI r_CATG'y
Cuts at: 1132 1229 2032 4166 5711 1132
Size: 97 803 2134 1545 2229
Fragments arranged by size:
2229 2134 1545 803 97
PasI CC'CwG_GG
Cuts at: 1348 2817 3800 1348
Size: 1469 983 4356
Fragments arranged by size:
4356 1469 983
PciI A'CATG_T
Cuts at: 5707 5707
Size: 6808
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3004 3367 1108
Size: 1896 363 4549
Fragments arranged by size:
4549 1896 363
PfoI T'CCnGG_A
Cuts at: 2694 3462 2694
Size: 768 6040
PmeI GTTT'AAAC
Cuts at: 4112 4112
Size: 6808
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4877 4909 6390 6422 4877
Size: 32 1481 32 5263
Fragments arranged by size:
5263 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2095 3287 3804 4045 2095
Size: 1192 517 241 4858
Fragments arranged by size:
4858 1192 517 241
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6808
PsiI TTA'TAA
Cuts at: 4378 4378
Size: 6808
PspOMI G'GGCC_C
Cuts at: 3138 3138
Size: 6808
PstI C_TGCA'G
Cuts at: 830 2106 2166 2178 2379 3150 4160 830
Size: 1276 60 12 201 771 1010 3478
Fragments arranged by size:
3478 1276 1010 771 201 60 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6808
PvuII CAG'CTG
Cuts at: 2020 2038 2326 3334 3452 3668 2020
Size: 18 288 1008 118 216 5160
Fragments arranged by size:
5160 1008 288 216 118 18
SacI G_AGCT'C
Cuts at: 2735 2735
Size: 6808
SalI G'TCGA_C
Cuts at: 1936 4150 1936
Size: 2214 4594
SanDI GG'GwC_CC
Cuts at: 3287 3804 4045 3287
Size: 517 241 6050
Fragments arranged by size:
6050 517 241
SbfI CC_TGCA'GG
Cuts at: 4160 4160
Size: 6808
ScaI AGT'ACT
Cuts at: 2630 5056 6768 2630
Size: 2426 1712 2670
Fragments arranged by size:
2670 2426 1712
SfcI C'TryA_G
Cuts at: 826 1045 2102 2162 2174 2375 3146 4156 5291
Size: 219 1057 60 12 201 771 1010 1135
Cuts at: 5291 5972 6163 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1057 1010 771 681 219 201
191 60 12
SfoI GGC'GCC
Cuts at: 3711 3902 3711
Size: 191 6617
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6808
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6808
SmaI CCC'GGG
Cuts at: 1872 2532 4135 1872
Size: 660 1603 4545
Fragments arranged by size:
4545 1603 660
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2468 2972 3047 4912 5813 6075
Size: 197 926 525 504 75 1865 901 262
Cuts at: 6075 820
Size: 1553
Fragments arranged by size:
1865 1553 926 901 525 504 262 197
75
SnaBI TAC'GTA
Cuts at: 493 4599 493
Size: 4106 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6808
SphI G_CATG'C
Cuts at: 1132 2032 4166 1132
Size: 900 2134 3774
Fragments arranged by size:
3774 2134 900
SspI AAT'ATT
Cuts at: 5 52 4732 5
Size: 47 4680 2081
Fragments arranged by size:
4680 2081 47
StuI AGG'CCT
Cuts at: 1517 2168 2294 2517 2888 3441 1517
Size: 651 126 223 371 553 4884
Fragments arranged by size:
4884 651 553 371 223 126
StyI C'CwwG_G
Cuts at: 513 1065 2295 2518 2553 2658 3576 3594 3639
Size: 552 1230 223 35 105 918 18 45
Cuts at: 3639 4247 513
Size: 608 3074
Fragments arranged by size:
3074 1230 918 608 552 223 105 45
35 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2078 2822 3277 4835 4852 5005 5190
Size: 1255 498 744 455 1558 17 153 185
Cuts at: 5190 5635 325
Size: 445 1498
Fragments arranged by size:
1558 1498 1255 744 498 455 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2628 5054 6766
Size: 274 80 33 51 1443 651 2426 1712
Cuts at: 6766 96
Size: 138
Fragments arranged by size:
2426 1712 1443 651 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3523 3555 3637 3669 6768 6800 3523
Size: 32 82 32 3099 32 3531
Fragments arranged by size:
3531 3099 82 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3470 3607 1316
Size: 2154 137 4517
Fragments arranged by size:
4517 2154 137
XbaI T'CTAG_A
Cuts at: 4144 4144
Size: 6808
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3364 3364
Size: 6808
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6808
XmaI C'CCGG_G
Cuts at: 1870 2530 4133 1870
Size: 660 1603 4545
Fragments arranged by size:
4545 1603 660
XmnI GAAnn'nnTTC
Cuts at: 4937 4937
Size: 6808
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2371 3827 4616 276
Size: 53 83 186 936 837 1456 789 2468
Fragments arranged by size:
2468 1456 936 837 789 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI