Restriction map for
pFN21AB5224
(Circular) (Six-base) MAPSORT of: pFN21AB5224.seq Check: 5045 from: 1 to: 5428
pFN21AB5224 5428 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3238 278
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2771 1937
Size: 834 4594
Acc65I G'GTAC_C
Cuts at: 2749 2749
Size: 5428
AclI AA'CG_TT
Cuts at: 1563 3555 3928 1563
Size: 1992 373 3063
Fragments arranged by size:
3063 1992 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3491 4875 5143 1632
Size: 1859 1384 268 1917
Fragments arranged by size:
1917 1859 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5231
AflIII A'CryG_T
Cuts at: 1203 4327 5304 1203
Size: 3124 977 1327
Fragments arranged by size:
3124 1327 977
AgeI A'CCGG_T
Cuts at: 4259 4259
Size: 5428
AhdI GACnn_n'nnGTC
Cuts at: 4157 4157
Size: 5428
AleI CACnn'nnGTG
Cuts at: 1166 2663 2696 1166
Size: 1497 33 3898
Fragments arranged by size:
3898 1497 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5428
AlwNI CAG_nnn'CTG
Cuts at: 2369 2545 3171 4743 2369
Size: 176 626 1572 3054
Fragments arranged by size:
3054 1572 626 176
ApaLI G'TGCA_C
Cuts at: 3485 4641 3485
Size: 1156 4272
ApoI r'AATT_y
Cuts at: 1493 2668 2737 2963 3115 5224 1493
Size: 1175 69 226 152 2109 1697
Fragments arranged by size:
2109 1697 1175 226 152 69
AseI AT'TA_AT
Cuts at: 160 1032 3982 160
Size: 872 2950 1606
Fragments arranged by size:
2950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
AvaI C'yCGr_G
Cuts at: 1870 1943 2753 1870
Size: 73 810 4545
Fragments arranged by size:
4545 810 73
BamHI G'GATC_C
Cuts at: 2758 3125 2758
Size: 367 5061
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2406 2749 4204 618
Size: 325 217 627 619 343 1455 1842
Fragments arranged by size:
1842 1455 627 619 343 325 217
BanII G_rGCy'C
Cuts at: 1391 2747 1391
Size: 1356 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2388 2585 928
Size: 971 489 197 3771
Fragments arranged by size:
3771 971 489 197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3619 3653 1903
Size: 34 1682 34 3678
Fragments arranged by size:
3678 1682 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3320 4530 3320
Size: 1210 4218
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2058 4039 136
Size: 107 122 71 631 605 386 1981 1525
Fragments arranged by size:
1981 1525 631 605 386 122 107 71
BglII A'GATC_T
Cuts at: 5423 5423
Size: 5428
BlpI GC'TnA_GC
Cuts at: 2707 2845 2707
Size: 138 5290
Bme1580I G_kGCm'C
Cuts at: 1790 3489 4645 1790
Size: 1699 1156 2573
Fragments arranged by size:
2573 1699 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2102 2270 2284
Size: 416 53 83 399 24 685 168 14
Cuts at: 2284 3159 4117 442
Size: 875 958 1753
Fragments arranged by size:
1753 958 875 685 416 399 168 83
53 24 14
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2083 2555 4088 1239
Size: 844 472 1533 2579
Fragments arranged by size:
2579 1533 844 472
Bpu10I CC'TnA_GC
Cuts at: 2480 2480
Size: 5428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3553 4418 4716 3553
Size: 865 298 4265
Fragments arranged by size:
4265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4091 882
Size: 617 2592 2219
Fragments arranged by size:
2592 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2698 3219 493
Size: 711 1494 521 2702
Fragments arranged by size:
2702 1494 711 521
BsaBI GATnn'nnATC
Cuts at: 5422 5422
Size: 5428
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3235 3617 4252 275
Size: 53 83 186 936 1702 382 635 1451
Fragments arranged by size:
1702 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2391 2508 3861 4259 4533 4680 5091 2391
Size: 117 1353 398 274 147 411 2728
Fragments arranged by size:
2728 1353 411 398 274 147 117
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5203
Fragments arranged by size:
5203 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2080 2516 2716 1186
Size: 894 436 200 3898
Fragments arranged by size:
3898 894 436 200
BseYI C'CCAG_C
Cuts at: 2018 4631 5127 2018
Size: 2613 496 2319
Fragments arranged by size:
2613 2319 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2029 1163
Size: 573 293 4562
Fragments arranged by size:
4562 573 293
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2498 2878 3639 4667 1540
Size: 275 186 497 380 761 1028 2301
Fragments arranged by size:
2301 1028 761 497 380 275 186
BsiHKAI G_wGCw'C
Cuts at: 2072 3489 3574 4645 2072
Size: 1417 85 1071 2855
Fragments arranged by size:
2855 1417 1071 85
BsmI GAATG_Cn'
Cuts at: 2939 3032 2939
Size: 93 5335
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2747 3489 3574 4645 1391
Size: 399 282 675 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 675 399 282 85
BspEI T'CCGG_A
Cuts at: 2391 5091 2391
Size: 2700 2728
BspHI T'CATG_A
Cuts at: 2025 3317 5047 2025
Size: 1292 1730 2406
Fragments arranged by size:
2406 1730 1292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2783 844
Size: 1939 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3315 1394
Size: 429 1492 3507
Fragments arranged by size:
3507 1492 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3923 4097 65
Size: 1183 2675 174 1396
Fragments arranged by size:
2675 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2057 2493 4072 4259 1700
Size: 110 247 436 1579 187 2869
Fragments arranged by size:
2869 1579 436 247 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5428
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5428
BssSI C'ACGA_G
Cuts at: 2267 3488 4500 2267
Size: 1221 1012 3195
Fragments arranged by size:
3195 1221 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2545 794
Size: 1751 3677
BstBI TT'CG_AA
Cuts at: 4232 4232
Size: 5428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5428
BstXI CCAn_nnnn'nTGG
Cuts at: 2533 2617 2533
Size: 84 5344
BstYI r'GATC_y
Cuts at: 1972 2758 3125 3510 3527 4979 5065 5077 5423
Size: 786 367 385 17 1452 86 12 346
Cuts at: 5423 1972
Size: 1977
Fragments arranged by size:
1977 1452 786 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2174 2174
Size: 5428
BtgI C'CryG_G
Cuts at: 513 1065 2142 513
Size: 552 1077 3799
Fragments arranged by size:
3799 1077 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4689
Fragments arranged by size:
4689 683 56
BtsI GCAGTG_nn'
Cuts at: 2526 2944 3738 3758 2526
Size: 418 794 20 4196
Fragments arranged by size:
4196 794 418 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5393
DraI TTT'AAA
Cuts at: 2732 2732
Size: 5428
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2153 4435 809
Size: 507 837 2282 1802
Fragments arranged by size:
2282 1802 837 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2523 2607 2619
Size: 54 1425 50 275 18 693 84 12
Cuts at: 2619 2875 3764 5393 8
Size: 256 889 1629 43
Fragments arranged by size:
1629 1425 889 693 275 256 84 54
50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2875 1537
Size: 275 1063 4090
Fragments arranged by size:
4090 1063 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3358 1353
Size: 2005 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4015 4389 4535 387
Size: 1064 138 2426 374 146 1280
Fragments arranged by size:
2426 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2083 2555 3491 4088 4875 5143 1239
Size: 393 451 472 936 597 787 268 1524
Fragments arranged by size:
1524 936 787 597 472 451 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2414 2698 2414
Size: 284 5144
EcoO109I rG'GnC_Cy
Cuts at: 2518 2518
Size: 5428
EcoRI G'AATT_C
Cuts at: 2737 3115 2737
Size: 378 5050
FspI TGC'GCA
Cuts at: 3934 3934
Size: 5428
HaeII r_GCGC'y
Cuts at: 1678 4575 1678
Size: 2897 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4069 4101 4143 4175 1373
Size: 32 135 32 2497 32 42 32 2626
Fragments arranged by size:
2626 2497 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2158 2772 3018 669
Size: 988 281 220 614 246 3079
Fragments arranged by size:
3079 988 614 281 246 220
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5428
HpaI GTT'AAC
Cuts at: 3018 3018
Size: 5428
KpnI G_GTAC'C
Cuts at: 2753 2753
Size: 5428
MfeI C'AATT_G
Cuts at: 3027 3027
Size: 5428
MluI A'CGCG_T
Cuts at: 5304 5304
Size: 5428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2099 2478 4542 4726 1305
Size: 794 379 2064 184 2007
Fragments arranged by size:
2064 2007 794 379 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2525 2609 2621 10
Size: 54 1425 343 693 84 12 2817
Fragments arranged by size:
2817 1425 693 343 84 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2588 2663 2696 3386 3745 3904 518
Size: 648 1422 75 33 690 359 159 2042
Fragments arranged by size:
2042 1422 690 648 359 159 75 33
MspA1I CmG'CkG
Cuts at: 1606 2013 2542 2844 3521 4669 4914 1606
Size: 407 529 302 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 529 407 302 245
NaeI GCC'GGC
Cuts at: 2059 2059
Size: 5428
NcoI C'CATG_G
Cuts at: 513 1065 2142 513
Size: 552 1077 3799
Fragments arranged by size:
3799 1077 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5428
NgoMIV G'CCGG_C
Cuts at: 2057 2057
Size: 5428
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5428
NotI GC'GGCC_GC
Cuts at: 2875 2875
Size: 5428
NspI r_CATG'y
Cuts at: 1132 1229 2786 4331 1132
Size: 97 1557 1545 2229
Fragments arranged by size:
2229 1557 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5428
PciI A'CATG_T
Cuts at: 4327 4327
Size: 5428
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2143 1108
Size: 1035 4393
PmeI GTTT'AAAC
Cuts at: 2732 2732
Size: 5428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 5010 5042 3497
Size: 32 1481 32 3883
Fragments arranged by size:
3883 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2518 2518
Size: 5428
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5428
PsiI TTA'TAA
Cuts at: 2998 2998
Size: 5428
PstI C_TGCA'G
Cuts at: 830 2316 2478 2550 2780 830
Size: 1486 162 72 230 3478
Fragments arranged by size:
3478 1486 230 162 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5428
PvuII CAG'CTG
Cuts at: 2542 2542
Size: 5428
SalI G'TCGA_C
Cuts at: 1936 2770 1936
Size: 834 4594
SbfI CC_TGCA'GG
Cuts at: 2780 2780
Size: 5428
ScaI AGT'ACT
Cuts at: 3676 5388 3676
Size: 1712 3716
SfcI C'TryA_G
Cuts at: 826 1045 2312 2474 2546 2776 3911 4592 4783
Size: 219 1267 162 72 230 1135 681 191
Cuts at: 4783 826
Size: 1471
Fragments arranged by size:
1471 1267 1135 681 230 219 191 162
72
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5428
SmaI CCC'GGG
Cuts at: 1872 2755 1872
Size: 883 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3532 4433 4695 820
Size: 197 926 1589 901 262 1553
Fragments arranged by size:
1589 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3219 493
Size: 2726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5428
SphI G_CATG'C
Cuts at: 1132 2786 1132
Size: 1654 3774
SspI AAT'ATT
Cuts at: 5 52 2398 3352 5
Size: 47 2346 954 2081
Fragments arranged by size:
2346 2081 954 47
StuI AGG'CCT
Cuts at: 1517 2552 1517
Size: 1035 4393
StyI C'CwwG_G
Cuts at: 513 1065 2142 2867 513
Size: 552 1077 725 3074
Fragments arranged by size:
3074 1077 725 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3455 3472 3625 3810 4255 325
Size: 1255 1875 17 153 185 445 1498
Fragments arranged by size:
1875 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3674 5386 96
Size: 274 80 33 51 1443 1697 1712 138
Fragments arranged by size:
1712 1697 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2618 2650 5388 5420 2618
Size: 32 2738 32 2626
Fragments arranged by size:
2738 2626 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5428
XbaI T'CTAG_A
Cuts at: 2764 2764
Size: 5428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2590 2719 2590
Size: 129 5299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5428
XmaI C'CCGG_G
Cuts at: 1870 2753 1870
Size: 883 4545
XmnI GAAnn'nnTTC
Cuts at: 3557 3557
Size: 5428
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3236 276
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI