Restriction map for
pFN21AB5212
(Circular) (Six-base) MAPSORT of: pFN21AB5212.seq Check: 7296 from: 1 to: 5887
pFN21AB5212 5887 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3104 3104
Size: 5887
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2112 3230 1937
Size: 175 1118 4594
Fragments arranged by size:
4594 1118 175
Acc65I G'GTAC_C
Cuts at: 3208 3208
Size: 5887
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3950 5334 5602 1632
Size: 2318 1384 268 1917
Fragments arranged by size:
2318 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 2664 3001 4786 5763 1203
Size: 1461 337 1785 977 1327
Fragments arranged by size:
1785 1461 1327 977 337
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 2171 1166
Size: 1005 4882
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2360 1184
Size: 1176 4711
AlwNI CAG_nnn'CTG
Cuts at: 2593 3630 5202 2593
Size: 1037 1572 3278
Fragments arranged by size:
3278 1572 1037
ApaLI G'TGCA_C
Cuts at: 3944 5100 3944
Size: 1156 4731
ApoI r'AATT_y
Cuts at: 1493 2438 2945 3196 3422 3574 5683 1493
Size: 945 507 251 226 152 2109 1697
Fragments arranged by size:
2109 1697 945 507 251 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4441 160
Size: 872 3409 1606
Fragments arranged by size:
3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2369 2981 3009 3212 1870
Size: 73 72 354 612 28 203 4545
Fragments arranged by size:
4545 612 354 203 73 72 28
BamHI G'GATC_C
Cuts at: 2187 2706 3217 3584 2187
Size: 519 511 367 4490
Fragments arranged by size:
4490 519 511 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2344 3208 4663 618
Size: 325 217 627 557 864 1455 1842
Fragments arranged by size:
1842 1455 864 627 557 325 217
BanII G_rGCy'C
Cuts at: 1391 2988 3206 1391
Size: 1597 218 4072
Fragments arranged by size:
4072 1597 218
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4916
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2711 2745 4078 4112 1903
Size: 34 774 34 1333 34 3678
Fragments arranged by size:
3678 1333 774 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3779 4989 3779
Size: 1210 4677
BclI T'GATC_A
Cuts at: 1548 2556 1548
Size: 1008 4879
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4498 136
Size: 107 122 71 631 605 2826 1525
Fragments arranged by size:
2826 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2887 5882 2887
Size: 2995 2892
BlpI GC'TnA_GC
Cuts at: 3304 3304
Size: 5887
Bme1580I G_kGCm'C
Cuts at: 1790 2349 2530 3948 5104 1790
Size: 559 181 1418 1156 2573
Fragments arranged by size:
2573 1418 1156 559 181
BmgBI CAC'GTC
Cuts at: 1318 2554 2674 1318
Size: 1236 120 4531
Fragments arranged by size:
4531 1236 120
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2524 3618 4576
Size: 416 53 83 399 24 1107 1094 958
Cuts at: 4576 442
Size: 1753
Fragments arranged by size:
1753 1107 1094 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2169 4547 1239
Size: 930 2378 2579
Fragments arranged by size:
2579 2378 930
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4012 4877 5175 4012
Size: 865 298 4724
Fragments arranged by size:
4724 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2624 4550 882
Size: 617 1125 1926 2219
Fragments arranged by size:
2219 1926 1125 617
BsaAI yAC'GTr
Cuts at: 493 1204 3678 493
Size: 711 2474 2702
Fragments arranged by size:
2702 2474 711
BsaBI GATnn'nnATC
Cuts at: 2186 5881 2186
Size: 3695 2192
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3070 3694 4076 4711
Size: 53 83 186 936 1537 624 382 635
Cuts at: 4711 275
Size: 1451
Fragments arranged by size:
1537 1451 936 635 624 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4320 4718 4992 5139 5550 4320
Size: 398 274 147 411 4657
Fragments arranged by size:
4657 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2483 2513 1178
Size: 30 165 30 1080 30 4552
Fragments arranged by size:
4552 1080 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 1186
Size: 877 5010
BseYI C'CCAG_C
Cuts at: 2140 2912 5090 5586 2140
Size: 772 2178 496 2441
Fragments arranged by size:
2441 2178 772 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 1163
Size: 573 564 4750
Fragments arranged by size:
4750 573 564
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3337 4098 5126 1540
Size: 275 186 1336 761 1028 2301
Fragments arranged by size:
2301 1336 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3948 4033 5104 3948
Size: 85 1071 4731
Fragments arranged by size:
4731 1071 85
BsmI GAATG_Cn'
Cuts at: 3398 3491 3398
Size: 93 5794
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2349 2530 2988 3206 3948 4033 5104
Size: 399 559 181 458 218 742 85 1071
Cuts at: 5104 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 559 458 399 218 181
85
BspEI T'CCGG_A
Cuts at: 5550 5550
Size: 5887
BspHI T'CATG_A
Cuts at: 3776 5506 3776
Size: 1730 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2098 2601 3104 3242 844
Size: 1254 503 503 138 3489
Fragments arranged by size:
3489 1254 503 503 138
BsrBI CCG'CTC
Cuts at: 1394 1823 3774 1394
Size: 429 1951 3507
Fragments arranged by size:
3507 1951 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4382 4556 65
Size: 1183 3134 174 1396
Fragments arranged by size:
3134 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4531 4718 1700
Size: 110 2721 187 2869
Fragments arranged by size:
2869 2721 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5887
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5887
BssSI C'ACGA_G
Cuts at: 3947 4959 3947
Size: 1012 4875
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2131 2890 794
Size: 1337 759 3791
Fragments arranged by size:
3791 1337 759
BstBI TT'CG_AA
Cuts at: 4691 4691
Size: 5887
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5887
BstYI r'GATC_y
Cuts at: 1972 2187 2706 2887 3217 3584 3969 3986 5438
Size: 215 519 181 330 367 385 17 1452
Cuts at: 5438 5524 5536 5882 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 519 385 367 346 330 215
181 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2967 2967
Size: 5887
BtgI C'CryG_G
Cuts at: 513 1065 2004 2352 513
Size: 552 939 348 4048
Fragments arranged by size:
4048 939 552 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2271 2703 487
Size: 683 56 1045 432 3671
Fragments arranged by size:
3671 1045 683 432 56
BtsI GCAGTG_nn'
Cuts at: 3051 3403 4197 4217 3051
Size: 352 794 20 4721
Fragments arranged by size:
4721 794 352 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5852
DraI TTT'AAA
Cuts at: 3191 3191
Size: 5887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2108 4894 809
Size: 507 792 2786 1802
Fragments arranged by size:
2786 1802 792 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 2361 2680
Size: 54 1425 50 275 18 495 36 319
Cuts at: 2680 2748 3334 4223 5852 8
Size: 68 586 889 1629 43
Fragments arranged by size:
1629 1425 889 586 495 319 275 68
54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2220 2825 3817 1353
Size: 867 605 992 3423
Fragments arranged by size:
3423 992 867 605
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3110 4474 4848 4994 387
Size: 1064 138 1521 1364 374 146 1280
Fragments arranged by size:
1521 1364 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2169 3950 4547 5334 5602 1239
Size: 393 537 1781 597 787 268 1524
Fragments arranged by size:
1781 1524 787 597 537 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2303 2804 2303
Size: 501 5386
EcoO109I rG'GnC_Cy
Cuts at: 2230 2590 2995 2230
Size: 360 405 5122
Fragments arranged by size:
5122 405 360
EcoRI G'AATT_C
Cuts at: 2438 3196 3574 2438
Size: 758 378 4751
Fragments arranged by size:
4751 758 378
FspI TGC'GCA
Cuts at: 2852 4393 2852
Size: 1541 4346
HaeII r_GCGC'y
Cuts at: 1678 5034 1678
Size: 3356 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4528 4560 4602 4634 1373
Size: 32 135 32 2956 32 42 32 2626
Fragments arranged by size:
2956 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2656 3231 3477 669
Size: 988 281 718 575 246 3079
Fragments arranged by size:
3079 988 718 575 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5887
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KpnI G_GTAC'C
Cuts at: 3212 3212
Size: 5887
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 3001 5763 3001
Size: 2762 3125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2526 5001 5185 1305
Size: 1221 2475 184 2007
Fragments arranged by size:
2475 2007 1221 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 2682 2750 10
Size: 54 1425 343 531 319 68 3147
Fragments arranged by size:
3147 1425 531 343 319 68 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2199 2741 2861 3845 4204 4363
Size: 648 1005 28 542 120 984 359 159
Cuts at: 4363 518
Size: 2042
Fragments arranged by size:
2042 1005 984 648 542 359 159 120
28
MspA1I CmG'CkG
Cuts at: 1606 2092 2515 3303 3980 5128 5373 1606
Size: 486 423 788 677 1148 245 2120
Fragments arranged by size:
2120 1148 788 677 486 423 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4396
Fragments arranged by size:
4396 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5887
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5887
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 2198 2453 2668 3163 3245 4790 1132
Size: 97 969 255 215 495 82 1545 2229
Fragments arranged by size:
2229 1545 969 495 255 215 97 82
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5887
PciI A'CATG_T
Cuts at: 2664 4786 2664
Size: 2122 3765
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5887
PfoI T'CCnGG_A
Cuts at: 2538 2775 2538
Size: 237 5650
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2363 2395 2621 2653 3956 3988 5469 5501 2363
Size: 32 226 32 1303 32 1481 32 2749
Fragments arranged by size:
2749 1481 1303 226 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2230 2590 2995 2230
Size: 360 405 5122
Fragments arranged by size:
5122 405 360
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PstI C_TGCA'G
Cuts at: 830 2097 2805 3239 830
Size: 1267 708 434 3478
Fragments arranged by size:
3478 1267 708 434
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
PvuII CAG'CTG
Cuts at: 2092 2515 2092
Size: 423 5464
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SbfI CC_TGCA'GG
Cuts at: 2805 3239 2805
Size: 434 5453
ScaI AGT'ACT
Cuts at: 2534 3020 4135 5847 2534
Size: 486 1115 1712 2574
Fragments arranged by size:
2574 1712 1115 486
SexAI A'CCwGG_T
Cuts at: 2340 2340
Size: 5887
SfcI C'TryA_G
Cuts at: 826 1045 2093 2801 3235 4370 5051 5242 826
Size: 219 1048 708 434 1135 681 191 1471
Fragments arranged by size:
1471 1135 1048 708 681 434 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3011 3214 1872
Size: 1139 203 4545
Fragments arranged by size:
4545 1139 203
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3991 4892 5154 820
Size: 197 926 2048 901 262 1553
Fragments arranged by size:
2048 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3678 493
Size: 3185 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 2453 3163 3245 1132
Size: 1321 710 82 3774
Fragments arranged by size:
3774 1321 710 82
SspI AAT'ATT
Cuts at: 5 52 3811 5
Size: 47 3759 2081
Fragments arranged by size:
3759 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5887
StyI C'CwwG_G
Cuts at: 513 1065 2004 2454 3326 513
Size: 552 939 450 872 3074
Fragments arranged by size:
3074 939 872 552 450
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 2132 2666 3914 3931 4084 4269
Size: 1255 465 87 534 1248 17 153 185
Cuts at: 4269 4714 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1248 534 465 445 185 153
87 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2532 3018 4133
Size: 274 80 33 51 1443 555 486 1115
Cuts at: 4133 5845 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1115 555 486 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5847 5879 5847
Size: 32 5855
Tth111I GACn'n_nGTC
Cuts at: 1316 2651 1316
Size: 1335 4552
XbaI T'CTAG_A
Cuts at: 3223 3223
Size: 5887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2338 2641 2740 2338
Size: 303 99 5485
Fragments arranged by size:
5485 303 99
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5887
XmaI C'CCGG_G
Cuts at: 1870 3009 3212 1870
Size: 1139 203 4545
Fragments arranged by size:
4545 1139 203
XmnI GAAnn'nnTTC
Cuts at: 2378 2819 4016 2378
Size: 441 1197 4249
Fragments arranged by size:
4249 1197 441
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI