Restriction map for
pFN21AB5211
(Circular) (Six-base) MAPSORT of: pFN21AB5211.seq Check: 1121 from: 1 to: 5839
pFN21AB5211 5839 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2309 2309
Size: 5839
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2406 2924 3649 278
Size: 53 83 186 936 870 518 725 2468
Fragments arranged by size:
2468 936 870 725 518 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3182 1937
Size: 1245 4594
Acc65I G'GTAC_C
Cuts at: 3160 3160
Size: 5839
AclI AA'CG_TT
Cuts at: 1563 2427 3966 4339 1563
Size: 864 1539 373 3063
Fragments arranged by size:
3063 1539 864 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2708 3135 3902 5286 5554 1632
Size: 1076 427 767 1384 268 1917
Fragments arranged by size:
1917 1384 1076 767 427 268
AfeI AGC'GCT
Cuts at: 2873 2873
Size: 5839
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5642
AflIII A'CryG_T
Cuts at: 1203 2486 2906 4738 5715 1203
Size: 1283 420 1832 977 1327
Fragments arranged by size:
1832 1327 1283 977 420
AgeI A'CCGG_T
Cuts at: 4670 4670
Size: 5839
AhdI GACnn_n'nnGTC
Cuts at: 4568 4568
Size: 5839
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5839
AlwNI CAG_nnn'CTG
Cuts at: 3582 5154 3582
Size: 1572 4267
ApaI G_GGCC'C
Cuts at: 2395 2395
Size: 5839
ApaLI G'TGCA_C
Cuts at: 3896 5052 3896
Size: 1156 4683
ApoI r'AATT_y
Cuts at: 1493 2708 2843 3148 3374 3526 5635 1493
Size: 1215 135 305 226 152 2109 1697
Fragments arranged by size:
2109 1697 1215 305 226 152 135
AseI AT'TA_AT
Cuts at: 160 1032 4393 160
Size: 872 3361 1606
Fragments arranged by size:
3361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
AvaI C'yCGr_G
Cuts at: 1870 1943 2386 2826 3164 1870
Size: 73 443 440 338 4545
Fragments arranged by size:
4545 443 440 338 73
BamHI G'GATC_C
Cuts at: 2283 3169 3536 2283
Size: 886 367 4586
Fragments arranged by size:
4586 886 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3160 4615 618
Size: 325 217 627 1373 1455 1842
Fragments arranged by size:
1842 1455 1373 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2395 2827 3158 1391
Size: 1004 432 331 4072
Fragments arranged by size:
4072 1004 432 331
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2450 2959 928
Size: 971 551 509 3808
Fragments arranged by size:
3808 971 551 509
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4030 4064 1903
Size: 34 2093 34 3678
Fragments arranged by size:
3678 2093 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2591 3731 4941 2591
Size: 1140 1210 3489
Fragments arranged by size:
3489 1210 1140
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5839
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4450 136
Size: 107 122 71 631 605 2778 1525
Fragments arranged by size:
2778 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5834 5834
Size: 5839
BlpI GC'TnA_GC
Cuts at: 2112 3256 2112
Size: 1144 4695
Bme1580I G_kGCm'C
Cuts at: 1790 2395 3900 5056 1790
Size: 605 1505 1156 2573
Fragments arranged by size:
2573 1505 1156 605
BmgBI CAC'GTC
Cuts at: 1318 2353 1318
Size: 1035 4804
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3570 4528 442
Size: 416 53 83 399 24 2153 958 1753
Fragments arranged by size:
2153 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2509 2878 3116 4499 1239
Size: 1270 369 238 1383 2579
Fragments arranged by size:
2579 1383 1270 369 238
Bpu10I CC'TnA_GC
Cuts at: 2478 2478
Size: 5839
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2383 2869 3964 4829 5127 2383
Size: 486 1095 865 298 3095
Fragments arranged by size:
3095 1095 865 486 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4502 882
Size: 617 3003 2219
Fragments arranged by size:
3003 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2280 2489 2909 3630 493
Size: 711 1076 209 420 721 2702
Fragments arranged by size:
2702 1076 721 711 420 209
BsaBI GATnn'nnATC
Cuts at: 5833 5833
Size: 5839
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 2403 2440 2921
Size: 53 83 186 936 636 234 37 481
Cuts at: 2921 3646 4028 4663 275
Size: 725 382 635 1451
Fragments arranged by size:
1451 936 725 636 635 481 382 234
186 83 53 37
BsaWI w'CCGG_w
Cuts at: 4272 4670 4944 5091 5502 4272
Size: 398 274 147 411 4609
Fragments arranged by size:
4609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2762 2792 1178
Size: 30 165 30 1359 30 4225
Fragments arranged by size:
4225 1359 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2906 3113 1186
Size: 1720 207 3912
Fragments arranged by size:
3912 1720 207
BseYI C'CCAG_C
Cuts at: 2373 2868 2938 5042 5538 2373
Size: 495 70 2104 496 2674
Fragments arranged by size:
2674 2104 496 495 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2572 3149 1163
Size: 573 836 577 3853
Fragments arranged by size:
3853 836 577 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3289 4050 5078 1540
Size: 275 186 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2864 3900 3985 5056 2864
Size: 1036 85 1071 3647
Fragments arranged by size:
3647 1071 1036 85
BsmI GAATG_Cn'
Cuts at: 2745 3350 3443 2745
Size: 605 93 5141
Fragments arranged by size:
5141 605 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2395 2827 2864 3158 3900 3985 5056
Size: 399 605 432 37 294 742 85 1071
Cuts at: 5056 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 605 432 399 294 85
37
BspEI T'CCGG_A
Cuts at: 5502 5502
Size: 5839
BspHI T'CATG_A
Cuts at: 2347 3728 5458 2347
Size: 1381 1730 2728
Fragments arranged by size:
2728 1730 1381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2309 2345 3194 844
Size: 1465 36 849 3489
Fragments arranged by size:
3489 1465 849 36
BsrBI CCG'CTC
Cuts at: 1394 1823 3726 1394
Size: 429 1903 3507
Fragments arranged by size:
3507 1903 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4334 4508 65
Size: 1183 3086 174 1396
Fragments arranged by size:
3086 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2185 3089 4483 4670 1700
Size: 110 375 904 1394 187 2869
Fragments arranged by size:
2869 1394 904 375 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5839
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5839
BssSI C'ACGA_G
Cuts at: 3899 4911 3899
Size: 1012 4827
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5839
BstBI TT'CG_AA
Cuts at: 4643 4643
Size: 5839
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5839
BstYI r'GATC_y
Cuts at: 1972 2068 2283 3169 3536 3921 3938 5390 5476
Size: 96 215 886 367 385 17 1452 86
Cuts at: 5476 5488 5834 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 886 385 367 346 215 96
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2093 2093
Size: 5839
BtgI C'CryG_G
Cuts at: 513 1065 2004 2397 513
Size: 552 939 393 3955
Fragments arranged by size:
3955 939 552 393
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5100
Fragments arranged by size:
5100 683 56
BtsI GCAGTG_nn'
Cuts at: 2196 3355 4149 4169 2196
Size: 1159 794 20 3866
Fragments arranged by size:
3866 1159 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5804
DraI TTT'AAA
Cuts at: 3143 3143
Size: 5839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4846 809
Size: 507 3530 1802
Fragments arranged by size:
3530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 3286 4175
Size: 54 1425 50 275 18 429 1027 889
Cuts at: 4175 5804 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1027 889 429 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3286 1537
Size: 275 1474 4090
Fragments arranged by size:
4090 1474 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2294 3769 1353
Size: 941 1475 3423
Fragments arranged by size:
3423 1475 941
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2932 4426 4800 4946 387
Size: 1064 138 1343 1494 374 146 1280
Fragments arranged by size:
1494 1343 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2509 2708 2878 3116 3135 3902 4499
Size: 393 877 199 170 238 19 767 597
Cuts at: 4499 5286 5554 1239
Size: 787 268 1524
Fragments arranged by size:
1524 877 787 767 597 393 268 238
199 170 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2316 2316
Size: 5839
EcoO109I rG'GnC_Cy
Cuts at: 2128 2391 2392 2128
Size: 263 1 5575
Fragments arranged by size:
5575 263 1
EcoRI G'AATT_C
Cuts at: 2843 3148 3526 2843
Size: 305 378 5156
Fragments arranged by size:
5156 378 305
EcoRV GAT'ATC
Cuts at: 2152 2152
Size: 5839
FspI TGC'GCA
Cuts at: 4345 4345
Size: 5839
HaeII r_GCGC'y
Cuts at: 1678 2856 2875 4986 1678
Size: 1178 19 2111 2531
Fragments arranged by size:
2531 2111 1178 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2498 2530 2614 2646 2762
Size: 32 135 32 926 32 84 32 116
Cuts at: 2762 2794 4480 4512 4554 4586 1373
Size: 32 1686 32 42 32 2626
Fragments arranged by size:
2626 1686 926 135 116 84 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2121 2444 3183 3429 669
Size: 988 281 183 323 739 246 3079
Fragments arranged by size:
3079 988 739 323 281 246 183
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5839
HpaI GTT'AAC
Cuts at: 3429 3429
Size: 5839
KpnI G_GTAC'C
Cuts at: 3164 3164
Size: 5839
MfeI C'AATT_G
Cuts at: 3438 3438
Size: 5839
MluI A'CGCG_T
Cuts at: 5715 5715
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4953 5137 1305
Size: 3648 184 2007
Fragments arranged by size:
3648 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 10
Size: 54 1425 343 429 3588
Fragments arranged by size:
3588 1425 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 3797 4156 4315 518
Size: 648 1386 1245 359 159 2042
Fragments arranged by size:
2042 1386 1245 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2373 2399 2529 3255 3932 5080 5325 1606
Size: 767 26 130 726 677 1148 245 2120
Fragments arranged by size:
2120 1148 767 726 677 245 130 26
NaeI GCC'GGC
Cuts at: 2187 3091 2187
Size: 904 4935
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4348
Fragments arranged by size:
4348 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5839
NgoMIV G'CCGG_C
Cuts at: 2185 3089 2185
Size: 904 4935
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5839
NotI GC'GGCC_GC
Cuts at: 3286 3286
Size: 5839
NspI r_CATG'y
Cuts at: 1132 1229 3197 4742 1132
Size: 97 1968 1545 2229
Fragments arranged by size:
2229 1968 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5839
PciI A'CATG_T
Cuts at: 4738 4738
Size: 5839
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2551 1108
Size: 1443 4396
PfoI T'CCnGG_A
Cuts at: 2797 2797
Size: 5839
PmeI GTTT'AAAC
Cuts at: 3143 3143
Size: 5839
PmlI CAC'GTG
Cuts at: 2280 2909 2280
Size: 629 5210
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 5421 5453 3908
Size: 32 1481 32 4294
Fragments arranged by size:
4294 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2128 2128
Size: 5839
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5839
PsiI TTA'TAA
Cuts at: 3409 3409
Size: 5839
PspOMI G'GGCC_C
Cuts at: 2391 2391
Size: 5839
PstI C_TGCA'G
Cuts at: 830 2372 2981 3191 830
Size: 1542 609 210 3478
Fragments arranged by size:
3478 1542 609 210
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5839
PvuII CAG'CTG
Cuts at: 2373 2373
Size: 5839
SacII CC_GC'GG
Cuts at: 2400 2400
Size: 5839
SalI G'TCGA_C
Cuts at: 1936 3181 1936
Size: 1245 4594
SbfI CC_TGCA'GG
Cuts at: 3191 3191
Size: 5839
ScaI AGT'ACT
Cuts at: 4087 5799 4087
Size: 1712 4127
SfcI C'TryA_G
Cuts at: 826 1045 2368 2977 3187 4322 5003 5194 826
Size: 219 1323 609 210 1135 681 191 1471
Fragments arranged by size:
1471 1323 1135 681 609 219 210 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5839
SmaI CCC'GGG
Cuts at: 1872 2388 2828 3166 1872
Size: 516 440 338 4545
Fragments arranged by size:
4545 516 440 338
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2362 2848 3943 4844 5106 820
Size: 197 926 419 486 1095 901 262 1553
Fragments arranged by size:
1553 1095 926 901 486 419 262 197
SnaBI TAC'GTA
Cuts at: 493 3630 493
Size: 3137 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5839
SphI G_CATG'C
Cuts at: 1132 3197 1132
Size: 2065 3774
SspI AAT'ATT
Cuts at: 5 52 3763 5
Size: 47 3711 2081
Fragments arranged by size:
3711 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5839
StyI C'CwwG_G
Cuts at: 513 1065 2004 2312 3278 513
Size: 552 939 308 966 3074
Fragments arranged by size:
3074 966 939 552 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2162 2650 3866 3883 4036 4221 4666
Size: 1255 582 488 1216 17 153 185 445
Cuts at: 4666 325
Size: 1498
Fragments arranged by size:
1498 1255 1216 582 488 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 2812 3118
Size: 274 80 33 51 1443 441 394 306
Cuts at: 3118 4085 5797 96
Size: 967 1712 138
Fragments arranged by size:
1712 1443 967 441 394 306 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5799 5831 5799
Size: 32 5807
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5839
XbaI T'CTAG_A
Cuts at: 3175 3175
Size: 5839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2277 2277
Size: 5839
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5839
XmaI C'CCGG_G
Cuts at: 1870 2386 2826 3164 1870
Size: 516 440 338 4545
Fragments arranged by size:
4545 516 440 338
XmnI GAAnn'nnTTC
Cuts at: 3968 3968
Size: 5839
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2404 2922 3647 276
Size: 53 83 186 936 870 518 725 2468
Fragments arranged by size:
2468 936 870 725 518 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FalI FseI
FspAI KasI NarI NruI NsiI PacI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI
SwaI