Restriction map for
pFN21AB5210
(Circular) (Six-base) MAPSORT of: pFN21AB5210.seq Check: 9694 from: 1 to: 5689
pFN21AB5210 5689 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2287 2287
Size: 5689
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2085 2397 3032 1937
Size: 148 312 635 4594
Fragments arranged by size:
4594 635 312 148
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2480 2723 2754 3752 5136 5404 1632
Size: 848 243 31 998 1384 268 1917
Fragments arranged by size:
1917 1384 998 848 268 243 31
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 2258 1166
Size: 1092 4597
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2190 1184
Size: 1006 4683
AlwNI CAG_nnn'CTG
Cuts at: 2542 2701 3432 5004 2542
Size: 159 731 1572 3227
Fragments arranged by size:
3227 1572 731 159
ApaLI G'TGCA_C
Cuts at: 2175 3746 4902 2175
Size: 1571 1156 2962
Fragments arranged by size:
2962 1571 1156
ApoI r'AATT_y
Cuts at: 1493 2998 3224 3376 5485 1493
Size: 1505 226 152 2109 1697
Fragments arranged by size:
2109 1697 1505 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2386 3014 1870
Size: 73 443 628 4545
Fragments arranged by size:
4545 628 443 73
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2263 2312 2594 3010
Size: 325 217 627 288 188 49 282 416
Cuts at: 3010 4465 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 416 325 288 282 217
188 49
BanII G_rGCy'C
Cuts at: 1391 2695 2808 3008 1391
Size: 1304 113 200 4072
Fragments arranged by size:
4072 1304 200 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2039 2687 928
Size: 971 140 648 3930
Fragments arranged by size:
3930 971 648 140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2816 4300 136
Size: 107 122 71 631 605 1144 1484 1525
Fragments arranged by size:
1525 1484 1144 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 2107 2145 2179 2268 2599 3750 4906 1790
Size: 317 38 34 89 331 1151 1156 2573
Fragments arranged by size:
2573 1156 1151 331 317 89 38 34
BmgBI CAC'GTC
Cuts at: 1318 2566 1318
Size: 1248 4441
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2034 2885 4349 1239
Size: 795 851 1464 2579
Fragments arranged by size:
2579 1464 851 795
Bpu10I CC'TnA_GC
Cuts at: 2694 2694
Size: 5689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2075 2285 2936 3814 4679 4977 2075
Size: 210 651 878 865 298 2787
Fragments arranged by size:
2787 878 865 651 298 210
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2029 2845 3480 493
Size: 711 825 816 635 2702
Fragments arranged by size:
2702 825 816 711 635
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2244 3496 3878 4513
Size: 53 83 186 936 711 1252 382 635
Cuts at: 4513 275
Size: 1451
Fragments arranged by size:
1451 1252 936 711 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2456 2486 1178
Size: 30 165 30 1053 30 4381
Fragments arranged by size:
4381 1053 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2182 1186
Size: 996 4693
BseYI C'CCAG_C
Cuts at: 2106 2267 2556 2937 2962 4892 5388 2106
Size: 161 289 381 25 1930 496 2407
Fragments arranged by size:
2407 1930 496 381 289 161 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2009 1163
Size: 573 273 4843
Fragments arranged by size:
4843 573 273
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2179 3750 3835 4906 2179
Size: 1571 85 1071 2962
Fragments arranged by size:
2962 1571 1071 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 2145 2179 2268 2599 2695 2808
Size: 399 317 38 34 89 331 96 113
Cuts at: 2808 3008 3750 3835 4906 1391
Size: 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 331 317 200 113
96 89 85 38 34
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2287 3044 844
Size: 1443 757 3489
Fragments arranged by size:
3489 1443 757
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2072 4333 4520 1700
Size: 110 262 2261 187 2869
Fragments arranged by size:
2869 2261 262 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 2897 3749 4761 2897
Size: 852 1012 3825
Fragments arranged by size:
3825 1012 852
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstXI CCAn_nnnn'nTGG
Cuts at: 2260 2671 2260
Size: 411 5278
BstYI r'GATC_y
Cuts at: 1972 2127 2793 3019 3386 3771 3788 5240 5326
Size: 155 666 226 367 385 17 1452 86
Cuts at: 5326 5338 5684 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 666 385 367 346 226 155
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2580 513
Size: 552 1515 3622
Fragments arranged by size:
3622 1515 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2476 2560 487
Size: 683 56 1250 84 3616
Fragments arranged by size:
3616 1250 683 84 56
BtsI GCAGTG_nn'
Cuts at: 3205 3999 4019 3205
Size: 794 20 4875
Fragments arranged by size:
4875 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2570 4696 809
Size: 507 1254 2126 1802
Fragments arranged by size:
2126 1802 1254 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2229 2361
Size: 54 1425 50 275 18 240 159 132
Cuts at: 2361 2760 2826 3136 4025 5654 8
Size: 399 66 310 889 1629 43
Fragments arranged by size:
1629 1425 889 399 310 275 240 159
132 66 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3619 1353
Size: 2266 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2480 2723 2754 2885 3752 4349
Size: 393 402 446 243 31 131 867 597
Cuts at: 4349 5136 5404 1239
Size: 787 268 1524
Fragments arranged by size:
1524 867 787 597 446 402 393 268
243 131 31
EcoO109I rG'GnC_Cy
Cuts at: 2368 2515 2668 2698 2368
Size: 147 153 30 5359
Fragments arranged by size:
5359 153 147 30
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2299 2951 4836 1678
Size: 621 652 1885 2531
Fragments arranged by size:
2531 1885 652 621
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4330 4362 4404 4436 1373
Size: 32 135 32 2758 32 42 32 2626
Fragments arranged by size:
2758 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2884 4803 4987 1305
Size: 1579 1919 184 2007
Fragments arranged by size:
2007 1919 1579 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 10
Size: 54 1425 343 531 3336
Fragments arranged by size:
3336 1425 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2258 2261 2579 3647 4006 4165 518
Size: 648 1092 3 318 1068 359 159 2042
Fragments arranged by size:
2042 1092 1068 648 359 318 159 3
MspA1I CmG'CkG
Cuts at: 1606 2831 2887 2941 2962 3105 3782 4930 5175
Size: 1225 56 54 21 143 677 1148 245
Cuts at: 5175 1606
Size: 2120
Fragments arranged by size:
2120 1225 1148 677 245 143 56 54
21
NaeI GCC'GGC
Cuts at: 2074 2074
Size: 5689
NcoI C'CATG_G
Cuts at: 513 1065 2580 513
Size: 552 1515 3622
Fragments arranged by size:
3622 1515 552
NdeI CA'TA_TG
Cuts at: 387 2224 387
Size: 1837 3852
NgoMIV G'CCGG_C
Cuts at: 2072 2072
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2671 1348
Size: 1323 4366
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PmlI CAC'GTG
Cuts at: 2029 2845 2029
Size: 816 4873
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PstI C_TGCA'G
Cuts at: 830 2547 3041 830
Size: 1717 494 3478
Fragments arranged by size:
3478 1717 494
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2941 2962 2941
Size: 21 5668
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 2543 2836 3037 4172 4853 5044 826
Size: 219 1498 293 201 1135 681 191 1471
Fragments arranged by size:
1498 1471 1135 681 293 219 201 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 2388 3016 1872
Size: 516 628 4545
Fragments arranged by size:
4545 628 516
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 2300 2951 3793 4694 4956
Size: 197 926 147 210 651 842 901 262
Cuts at: 4956 820
Size: 1553
Fragments arranged by size:
1553 926 901 842 651 262 210 197
147
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 2364 2580 2664 2985 3128 513
Size: 552 1299 216 84 321 143 3074
Fragments arranged by size:
3074 1299 552 321 216 143 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 3716 3733 3886 4071 4516 325
Size: 1255 741 1395 17 153 185 445 1498
Fragments arranged by size:
1498 1395 1255 741 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3935 5647 96
Size: 274 80 33 51 1443 1958 1712 138
Fragments arranged by size:
1958 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2456 2488 5649 5681 2456
Size: 32 3161 32 2464
Fragments arranged by size:
3161 2464 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2323 2323
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 2386 3014 1870
Size: 516 628 4545
Fragments arranged by size:
4545 628 516
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI