Restriction map for
pFN21AB5208
(Circular) (Six-base) MAPSORT of: pFN21AB5208.seq Check: 5257 from: 1 to: 5812
pFN21AB5208 5812 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2299 2299
Size: 5812
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3622 278
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3155 1937
Size: 1218 4594
Acc65I G'GTAC_C
Cuts at: 2659 3133 2659
Size: 474 5338
AclI AA'CG_TT
Cuts at: 1563 2832 3939 4312 1563
Size: 1269 1107 373 3063
Fragments arranged by size:
3063 1269 1107 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3875 5259 5527 1632
Size: 2243 1384 268 1917
Fragments arranged by size:
2243 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5615
AflIII A'CryG_T
Cuts at: 1203 4711 5688 1203
Size: 3508 977 1327
Fragments arranged by size:
3508 1327 977
AgeI A'CCGG_T
Cuts at: 4643 4643
Size: 5812
AhdI GACnn_n'nnGTC
Cuts at: 4541 4541
Size: 5812
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5812
AlwNI CAG_nnn'CTG
Cuts at: 3555 5127 3555
Size: 1572 4240
ApaLI G'TGCA_C
Cuts at: 3869 5025 3869
Size: 1156 4656
ApoI r'AATT_y
Cuts at: 1493 3121 3347 3499 5608 1493
Size: 1628 226 152 2109 1697
Fragments arranged by size:
2109 1697 1628 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4366 160
Size: 872 3334 1606
Fragments arranged by size:
3334 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
AvaI C'yCGr_G
Cuts at: 1870 1943 3137 1870
Size: 73 1194 4545
Fragments arranged by size:
4545 1194 73
BamHI G'GATC_C
Cuts at: 3142 3509 3142
Size: 367 5445
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2659 3133 4588 618
Size: 325 217 627 872 474 1455 1842
Fragments arranged by size:
1842 1455 872 627 474 325 217
BanII G_rGCy'C
Cuts at: 1391 2277 2553 2735 3131 1391
Size: 886 276 182 396 4072
Fragments arranged by size:
4072 886 396 276 182
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2234 2788 928
Size: 971 335 554 3952
Fragments arranged by size:
3952 971 554 335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4003 4037 1903
Size: 34 2066 34 3678
Fragments arranged by size:
3678 2066 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3704 4914 3704
Size: 1210 4602
BclI T'GATC_A
Cuts at: 1548 2487 1548
Size: 939 4873
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4423 136
Size: 107 122 71 631 605 2751 1525
Fragments arranged by size:
2751 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5807 5807
Size: 5812
BlpI GC'TnA_GC
Cuts at: 3229 3229
Size: 5812
Bme1580I G_kGCm'C
Cuts at: 1790 3873 5029 1790
Size: 2083 1156 2573
Fragments arranged by size:
2573 2083 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5812
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3543 4501 442
Size: 416 53 83 399 24 2126 958 1753
Fragments arranged by size:
2126 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4472 1239
Size: 3233 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3937 4802 5100 3937
Size: 865 298 4649
Fragments arranged by size:
4649 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2461 3053 3062 3087 4475 882
Size: 617 962 592 9 25 1388 2219
Fragments arranged by size:
2219 1388 962 617 592 25 9
BsaAI yAC'GTr
Cuts at: 493 1204 3603 493
Size: 711 2399 2702
Fragments arranged by size:
2702 2399 711
BsaBI GATnn'nnATC
Cuts at: 5806 5806
Size: 5812
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3619 4001 4636 275
Size: 53 83 186 936 2086 382 635 1451
Fragments arranged by size:
2086 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2553 4245 4643 4917 5064 5475 2553
Size: 1692 398 274 147 411 2890
Fragments arranged by size:
2890 1692 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3016 3046 1178
Size: 30 165 30 1613 30 3944
Fragments arranged by size:
3944 1613 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2479 2725 3074 1186
Size: 1293 246 349 3924
Fragments arranged by size:
3924 1293 349 246
BseYI C'CCAG_C
Cuts at: 2284 2546 2866 5015 5511 2284
Size: 262 320 2149 496 2585
Fragments arranged by size:
2585 2149 496 320 262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2566 1163
Size: 573 830 4409
Fragments arranged by size:
4409 830 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2422 3262 4023 5051 1540
Size: 275 186 421 840 761 1028 2301
Fragments arranged by size:
2301 1028 840 761 421 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 3873 3958 5029 2277
Size: 1596 85 1071 3060
Fragments arranged by size:
3060 1596 1071 85
BsmI GAATG_Cn'
Cuts at: 3323 3416 3323
Size: 93 5719
BsmBI CGTCTCn'nnnn_
Cuts at: 2848 2848
Size: 5812
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2553 2735 3131 3873 3958 5029
Size: 399 487 276 182 396 742 85 1071
Cuts at: 5029 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 487 399 396 276 182
85
BspEI T'CCGG_A
Cuts at: 2553 5475 2553
Size: 2922 2890
BspHI T'CATG_A
Cuts at: 2358 2409 2535 3701 5431 2358
Size: 51 126 1166 1730 2739
Fragments arranged by size:
2739 1730 1166 126 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2299 3167 844
Size: 1455 868 3489
Fragments arranged by size:
3489 1455 868
BsrBI CCG'CTC
Cuts at: 1394 1823 3003 3022 3699 1394
Size: 429 1180 19 677 3507
Fragments arranged by size:
3507 1180 677 429 19
BsrDI GCAATG_nn'
Cuts at: 65 1248 2137 4307 4481 65
Size: 1183 889 2170 174 1396
Fragments arranged by size:
2170 1396 1183 889 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4456 4643 1700
Size: 110 2646 187 2869
Fragments arranged by size:
2869 2646 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5812
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5812
BssSI C'ACGA_G
Cuts at: 3872 4884 3872
Size: 1012 4800
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2397 794
Size: 1603 4209
BstBI TT'CG_AA
Cuts at: 4616 4616
Size: 5812
BstEII G'GTnAC_C
Cuts at: 1184 2764 1184
Size: 1580 4232
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2788 2011
Size: 777 5035
BstYI r'GATC_y
Cuts at: 1972 3142 3509 3894 3911 5363 5449 5461 5807
Size: 1170 367 385 17 1452 86 12 346
Cuts at: 5807 1972
Size: 1977
Fragments arranged by size:
1977 1452 1170 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2493 2847 3063 513
Size: 552 939 489 354 216 3262
Fragments arranged by size:
3262 939 552 489 354 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2248 487
Size: 683 56 1022 4051
Fragments arranged by size:
4051 1022 683 56
BtsI GCAGTG_nn'
Cuts at: 3328 4122 4142 3328
Size: 794 20 4998
Fragments arranged by size:
4998 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5777
DraI TTT'AAA
Cuts at: 3116 3116
Size: 5812
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4819 809
Size: 507 3503 1802
Fragments arranged by size:
3503 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2454 2478
Size: 54 1425 50 275 18 429 195 24
Cuts at: 2478 2594 2850 3259 4148 5777 8
Size: 116 256 409 889 1629 43
Fragments arranged by size:
1629 1425 889 429 409 275 256 195
116 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3259 1537
Size: 275 1447 4090
Fragments arranged by size:
4090 1447 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2282 2960 3742 1353
Size: 929 678 782 3423
Fragments arranged by size:
3423 929 782 678
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 4399 4773 4919 387
Size: 1064 138 441 2369 374 146 1280
Fragments arranged by size:
2369 1280 1064 441 374 146 138
EcoICRI GAG'CTC
Cuts at: 2275 2275
Size: 5812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3875 4472 5259 5527 1239
Size: 393 2243 597 787 268 1524
Fragments arranged by size:
2243 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2740 2740
Size: 5812
EcoO109I rG'GnC_Cy
Cuts at: 3078 3078
Size: 5812
EcoRI G'AATT_C
Cuts at: 3121 3499 3121
Size: 378 5434
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2986 2986
Size: 5812
FspI TGC'GCA
Cuts at: 4318 4318
Size: 5812
HaeII r_GCGC'y
Cuts at: 1678 4959 1678
Size: 3281 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4453 4485 4527 4559 1373
Size: 32 135 32 2881 32 42 32 2626
Fragments arranged by size:
2881 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2085 3156 3402 669
Size: 988 281 147 1071 246 3079
Fragments arranged by size:
3079 1071 988 281 246 147
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5812
HpaI GTT'AAC
Cuts at: 3402 3402
Size: 5812
KpnI G_GTAC'C
Cuts at: 2663 3137 2663
Size: 474 5338
MfeI C'AATT_G
Cuts at: 3411 3411
Size: 5812
MluI A'CGCG_T
Cuts at: 5688 5688
Size: 5812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4926 5110 1305
Size: 3621 184 2007
Fragments arranged by size:
3621 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 2596 2852 10
Size: 54 1425 343 429 335 256 2970
Fragments arranged by size:
2970 1425 429 343 335 256 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2363 2498 2705 2771 2786 3770
Size: 648 843 354 135 207 66 15 984
Cuts at: 3770 4129 4288 518
Size: 359 159 2042
Fragments arranged by size:
2042 984 843 648 359 354 207 159
135 66 15
MspA1I CmG'CkG
Cuts at: 1606 2580 2919 3228 3905 5053 5298 1606
Size: 974 339 309 677 1148 245 2120
Fragments arranged by size:
2120 1148 974 677 339 309 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2493 2847 513
Size: 552 939 489 354 3478
Fragments arranged by size:
3478 939 552 489 354
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5812
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5812
NotI GC'GGCC_GC
Cuts at: 3259 3259
Size: 5812
NspI r_CATG'y
Cuts at: 1132 1229 2884 3170 4715 1132
Size: 97 1655 286 1545 2229
Fragments arranged by size:
2229 1655 1545 286 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5812
PciI A'CATG_T
Cuts at: 4711 4711
Size: 5812
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2030 2592 1108
Size: 922 562 4328
Fragments arranged by size:
4328 922 562
PmeI GTTT'AAAC
Cuts at: 3116 3116
Size: 5812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 5394 5426 3881
Size: 32 1481 32 4267
Fragments arranged by size:
4267 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3078 3078
Size: 5812
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5812
PsiI TTA'TAA
Cuts at: 3382 3382
Size: 5812
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2609 2641 2609
Size: 32 5780
PstI C_TGCA'G
Cuts at: 830 3164 830
Size: 2334 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5812
PvuII CAG'CTG
Cuts at: 2580 2919 2580
Size: 339 5473
SacI G_AGCT'C
Cuts at: 2277 2277
Size: 5812
SalI G'TCGA_C
Cuts at: 1936 3154 1936
Size: 1218 4594
SapI GCTCTTCn'nnn_
Cuts at: 2282 2960 2282
Size: 678 5134
SbfI CC_TGCA'GG
Cuts at: 3164 3164
Size: 5812
ScaI AGT'ACT
Cuts at: 4060 5772 4060
Size: 1712 4100
SfcI C'TryA_G
Cuts at: 826 1045 3160 4295 4976 5167 826
Size: 219 2115 1135 681 191 1471
Fragments arranged by size:
2115 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5812
SmaI CCC'GGG
Cuts at: 1872 3139 1872
Size: 1267 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3916 4817 5079 820
Size: 197 926 1973 901 262 1553
Fragments arranged by size:
1973 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3603 493
Size: 3110 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5812
SphI G_CATG'C
Cuts at: 1132 3170 1132
Size: 2038 3774
SspI AAT'ATT
Cuts at: 5 52 3736 5
Size: 47 3684 2081
Fragments arranged by size:
3684 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5812
StyI C'CwwG_G
Cuts at: 513 1065 2004 2493 2847 3251 513
Size: 552 939 489 354 404 3074
Fragments arranged by size:
3074 939 552 489 404 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2436 3839 3856 4009 4194 4639 325
Size: 1255 856 1403 17 153 185 445 1498
Fragments arranged by size:
1498 1403 1255 856 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4058 5770 96
Size: 274 80 33 51 1443 2081 1712 138
Fragments arranged by size:
2081 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2712 2744 5772 5804 2712
Size: 32 3028 32 2720
Fragments arranged by size:
3028 2720 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5812
XbaI T'CTAG_A
Cuts at: 3148 3148
Size: 5812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2330 2350 2330
Size: 20 5792
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5812
XmaI C'CCGG_G
Cuts at: 1870 3137 1870
Size: 1267 4545
XmnI GAAnn'nnTTC
Cuts at: 2969 3941 2969
Size: 972 4840
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3620 276
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI