Restriction map for
pFN21AB5205
(Circular) (Six-base) MAPSORT of: pFN21AB5205.seq Check: 9716 from: 1 to: 6334
pFN21AB5205 6334 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2314 2314
Size: 6334
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 278
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2244 3435 3677 1937
Size: 307 1191 242 4594
Fragments arranged by size:
4594 1191 307 242
Acc65I G'GTAC_C
Cuts at: 3655 3655
Size: 6334
AclI AA'CG_TT
Cuts at: 1563 4461 4834 1563
Size: 2898 373 3063
Fragments arranged by size:
3063 2898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 2981 4397 5781 6049 1632
Size: 707 642 1416 1384 268 1917
Fragments arranged by size:
1917 1416 1384 707 642 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6137
AflIII A'CryG_T
Cuts at: 1203 2925 5233 6210 1203
Size: 1722 2308 977 1327
Fragments arranged by size:
2308 1722 1327 977
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 5063 5063
Size: 6334
AleI CACnn'nnGTG
Cuts at: 1166 2291 1166
Size: 1125 5209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6334
AlwNI CAG_nnn'CTG
Cuts at: 2088 3301 3524 4077 5649 2088
Size: 1213 223 553 1572 2773
Fragments arranged by size:
2773 1572 1213 553 223
ApaLI G'TGCA_C
Cuts at: 4391 5547 4391
Size: 1156 5178
ApoI r'AATT_y
Cuts at: 1493 3643 3869 4021 6130 1493
Size: 2150 226 152 2109 1697
Fragments arranged by size:
2150 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 2618 2735 3659 1870
Size: 73 675 117 924 4545
Fragments arranged by size:
4545 924 675 117 73
BamHI G'GATC_C
Cuts at: 3664 4031 3664
Size: 367 5967
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2282 3134 3655 5110 618
Size: 325 217 627 495 852 521 1455 1842
Fragments arranged by size:
1842 1455 852 627 521 495 325 217
BanII G_rGCy'C
Cuts at: 1391 2498 2617 2736 3403 3653 1391
Size: 1107 119 119 667 250 4072
Fragments arranged by size:
4072 1107 667 250 119 119
BbeI G_GCGC'C
Cuts at: 3138 3138
Size: 6334
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2663 928
Size: 971 764 4599
Fragments arranged by size:
4599 971 764
BbvCI CC'TCA_GC
Cuts at: 2103 2268 2103
Size: 165 6169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3437 3471 4525 4559 1903
Size: 34 1500 34 1054 34 3678
Fragments arranged by size:
3678 1500 1054 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4226 5436 4226
Size: 1210 5124
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6334
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3527 4945 136
Size: 107 122 71 631 605 1855 1418 1525
Fragments arranged by size:
1855 1525 1418 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6329 6329
Size: 6334
BlpI GC'TnA_GC
Cuts at: 3751 3751
Size: 6334
Bme1580I G_kGCm'C
Cuts at: 1790 4395 5551 1790
Size: 2605 1156 2573
Fragments arranged by size:
2605 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6334
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2899 4065 5023
Size: 416 53 83 399 24 1482 1166 958
Cuts at: 5023 442
Size: 1753
Fragments arranged by size:
1753 1482 1166 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2877 4994 1239
Size: 1638 2117 2579
Fragments arranged by size:
2579 2117 1638
Bpu10I CC'TnA_GC
Cuts at: 2103 2268 2103
Size: 165 6169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3259 4459 5324 5622 3259
Size: 1200 865 298 3971
Fragments arranged by size:
3971 1200 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3047 4997 882
Size: 617 1548 1950 2219
Fragments arranged by size:
2219 1950 1548 617
BsaAI yAC'GTr
Cuts at: 493 1204 4125 493
Size: 711 2921 2702
Fragments arranged by size:
2921 2702 711
BsaBI GATnn'nnATC
Cuts at: 2591 6328 2591
Size: 3737 2597
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3135 4141 4523 5158
Size: 53 83 186 936 1602 1006 382 635
Cuts at: 5158 275
Size: 1451
Fragments arranged by size:
1602 1451 1006 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4767 5165 5439 5586 5997 4767
Size: 398 274 147 411 5104
Fragments arranged by size:
5104 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2850 2880 1178
Size: 30 165 30 1447 30 4632
Fragments arranged by size:
4632 1447 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2966 3167 1186
Size: 1780 201 4353
Fragments arranged by size:
4353 1780 201
BseYI C'CCAG_C
Cuts at: 2064 2391 2511 2672 3028 5537 6033 2064
Size: 327 120 161 356 2509 496 2365
Fragments arranged by size:
2509 2365 496 356 327 161 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5761
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3784 4545 5573 1540
Size: 275 186 1783 761 1028 2301
Fragments arranged by size:
2301 1783 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2498 2736 4395 4480 5551 2498
Size: 238 1659 85 1071 3281
Fragments arranged by size:
3281 1659 1071 238 85
BsmI GAATG_Cn'
Cuts at: 2648 3013 3845 3938 2648
Size: 365 832 93 5044
Fragments arranged by size:
5044 832 365 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2498 2617 2736 3403 3653 4395 4480
Size: 399 708 119 119 667 250 742 85
Cuts at: 4480 5551 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 708 667 399 250 119
119 85
BspEI T'CCGG_A
Cuts at: 5997 5997
Size: 6334
BspHI T'CATG_A
Cuts at: 3183 4223 5953 3183
Size: 1040 1730 3564
Fragments arranged by size:
3564 1730 1040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2314 3270 3689 844
Size: 1470 956 419 3489
Fragments arranged by size:
3489 1470 956 419
BsrBI CCG'CTC
Cuts at: 1394 1823 2489 3163 3576 4221 1394
Size: 429 666 674 413 645 3507
Fragments arranged by size:
3507 674 666 645 429 413
BsrDI GCAATG_nn'
Cuts at: 65 1248 2247 3453 4829 5003 65
Size: 1183 999 1206 1376 174 1396
Fragments arranged by size:
1396 1376 1206 1183 999 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4978 5165 1700
Size: 110 3168 187 2869
Fragments arranged by size:
3168 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6334
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6334
BssSI C'ACGA_G
Cuts at: 4394 5406 4394
Size: 1012 5322
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6334
BstBI TT'CG_AA
Cuts at: 5138 5138
Size: 6334
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6334
BstXI CCAn_nnnn'nTGG
Cuts at: 2395 2855 3244 3328 2395
Size: 460 389 84 5401
Fragments arranged by size:
5401 460 389 84
BstYI r'GATC_y
Cuts at: 1972 2844 2956 3664 4031 4416 4433 5885 5971
Size: 872 112 708 367 385 17 1452 86
Cuts at: 5971 5983 6329 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 872 708 385 367 346 112
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2949 2949
Size: 6334
BtgI C'CryG_G
Cuts at: 513 1065 2131 3127 3613 513
Size: 552 1066 996 486 3234
Fragments arranged by size:
3234 1066 996 552 486
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5595
Fragments arranged by size:
5595 683 56
BtsI GCAGTG_nn'
Cuts at: 2556 3850 4644 4664 2556
Size: 1294 794 20 4226
Fragments arranged by size:
4226 1294 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6299
DraI TTT'AAA
Cuts at: 3638 3638
Size: 6334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3163 5341 809
Size: 507 1847 2178 1802
Fragments arranged by size:
2178 1847 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2898 2938 3622
Size: 54 1425 50 275 18 1068 40 684
Cuts at: 3622 3781 4670 6299 8
Size: 159 889 1629 43
Fragments arranged by size:
1629 1425 1068 889 684 275 159 54
50 43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 3781 1537
Size: 275 1969 4090
Fragments arranged by size:
4090 1969 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2957 3045 4264 1353
Size: 1604 88 1219 3423
Fragments arranged by size:
3423 1604 1219 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4921 5295 5441 387
Size: 1064 138 3332 374 146 1280
Fragments arranged by size:
3332 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2496 2734 2496
Size: 238 6096
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2877 2981 4397 4994 5781 6049
Size: 393 707 538 104 1416 597 787 268
Cuts at: 6049 1239
Size: 1524
Fragments arranged by size:
1524 1416 787 707 597 538 393 268
104
EcoO109I rG'GnC_Cy
Cuts at: 2126 2126
Size: 6334
EcoRI G'AATT_C
Cuts at: 3643 4021 3643
Size: 378 5956
FspI TGC'GCA
Cuts at: 4840 4840
Size: 6334
HaeII r_GCGC'y
Cuts at: 1678 3138 5481 1678
Size: 1460 2343 2531
Fragments arranged by size:
2531 2343 1460
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2976 3008 3423 3455 4975
Size: 32 135 32 1404 32 415 32 1520
Cuts at: 4975 5007 5049 5081 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1520 1404 415 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2404 3678 3924 669
Size: 988 281 466 1274 246 3079
Fragments arranged by size:
3079 1274 988 466 281 246
HindIII A'AGCT_T
Cuts at: 748 2144 2520 3471 748
Size: 1396 376 951 3611
Fragments arranged by size:
3611 1396 951 376
HpaI GTT'AAC
Cuts at: 2404 3924 2404
Size: 1520 4814
KasI G'GCGC_C
Cuts at: 3134 3134
Size: 6334
KpnI G_GTAC'C
Cuts at: 3659 3659
Size: 6334
MfeI C'AATT_G
Cuts at: 2799 3933 2799
Size: 1134 5200
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5448 5632 1305
Size: 4143 184 2007
Fragments arranged by size:
4143 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2900 10
Size: 54 1425 343 1068 3444
Fragments arranged by size:
3444 1425 1068 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2291 3242 4292 4651 4810 518
Size: 648 1125 951 1050 359 159 2042
Fragments arranged by size:
2042 1125 1050 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2395 2632 3301 3349 3750 4427 5575 5820
Size: 789 237 669 48 401 677 1148 245
Cuts at: 5820 1606
Size: 2120
Fragments arranged by size:
2120 1148 789 677 669 401 245 237
48
NarI GG'CG_CC
Cuts at: 3135 3135
Size: 6334
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5782
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6334
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6334
NotI GC'GGCC_GC
Cuts at: 3781 3781
Size: 6334
NspI r_CATG'y
Cuts at: 1132 1229 2929 3114 3410 3692 5237 1132
Size: 97 1700 185 296 282 1545 2229
Fragments arranged by size:
2229 1700 1545 296 282 185 97
PasI CC'CwG_GG
Cuts at: 1348 2122 3412 1348
Size: 774 1290 4270
Fragments arranged by size:
4270 1290 774
PciI A'CATG_T
Cuts at: 2925 5233 2925
Size: 2308 4026
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6334
PfoI T'CCnGG_A
Cuts at: 3286 3286
Size: 6334
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3560 3592 4403 4435 5916 5948 3560
Size: 32 811 32 1481 32 3946
Fragments arranged by size:
3946 1481 811 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2126 2126
Size: 6334
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6334
PsiI TTA'TAA
Cuts at: 3904 3904
Size: 6334
PspXI vC'TCGA_Gb
Cuts at: 2618 2735 2618
Size: 117 6217
PstI C_TGCA'G
Cuts at: 830 2230 2311 2577 3686 830
Size: 1400 81 266 1109 3478
Fragments arranged by size:
3478 1400 1109 266 81
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
PvuII CAG'CTG
Cuts at: 2395 2632 3301 3349 2395
Size: 237 669 48 5380
Fragments arranged by size:
5380 669 237 48
SacI G_AGCT'C
Cuts at: 2498 2736 2498
Size: 238 6096
SalI G'TCGA_C
Cuts at: 1936 3676 1936
Size: 1740 4594
SanDI GG'GwC_CC
Cuts at: 2126 2126
Size: 6334
SbfI CC_TGCA'GG
Cuts at: 3686 3686
Size: 6334
ScaI AGT'ACT
Cuts at: 2909 4582 6294 2909
Size: 1673 1712 2949
Fragments arranged by size:
2949 1712 1673
SfcI C'TryA_G
Cuts at: 826 1045 2226 2307 2573 3549 3682 4817 5498
Size: 219 1181 81 266 976 133 1135 681
Cuts at: 5498 5689 826
Size: 191 1471
Fragments arranged by size:
1471 1181 1135 976 681 266 219 191
133 81
SfoI GGC'GCC
Cuts at: 3136 3136
Size: 6334
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6334
SmaI CCC'GGG
Cuts at: 1872 3661 1872
Size: 1789 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2618 2735 3274 4438 5339 5601
Size: 197 926 675 117 539 1164 901 262
Cuts at: 5601 820
Size: 1553
Fragments arranged by size:
1553 1164 926 901 675 539 262 197
117
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6334
SphI G_CATG'C
Cuts at: 1132 3410 3692 1132
Size: 2278 282 3774
Fragments arranged by size:
3774 2278 282
SspI AAT'ATT
Cuts at: 5 52 4258 5
Size: 47 4206 2081
Fragments arranged by size:
4206 2081 47
StuI AGG'CCT
Cuts at: 1517 3326 1517
Size: 1809 4525
StyI C'CwwG_G
Cuts at: 513 1065 2053 3099 3321 3327 3773 513
Size: 552 988 1046 222 6 446 3074
Fragments arranged by size:
3074 1046 988 552 446 222 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2264 2405 2918 4361 4378 4531 4716
Size: 1255 684 141 513 1443 17 153 185
Cuts at: 4716 5161 325
Size: 445 1498
Fragments arranged by size:
1498 1443 1255 684 513 445 185 153
141 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2907 4580 6292
Size: 274 80 33 51 1443 930 1673 1712
Cuts at: 6292 96
Size: 138
Fragments arranged by size:
1712 1673 1443 930 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2007 2039 6294 6326 2007
Size: 32 4255 32 2015
Fragments arranged by size:
4255 2015 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6334
XbaI T'CTAG_A
Cuts at: 3670 3670
Size: 6334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2893 2893
Size: 6334
XhoI C'TCGA_G
Cuts at: 1943 2618 2735 1943
Size: 675 117 5542
Fragments arranged by size:
5542 675 117
XmaI C'CCGG_G
Cuts at: 1870 3659 1870
Size: 1789 4545
XmnI GAAnn'nnTTC
Cuts at: 2522 2660 4463 2522
Size: 138 1803 4393
Fragments arranged by size:
4393 1803 138
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 276
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI
PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI