Restriction map for
pFN21AB5186
(Circular) (Six-base) MAPSORT of: pFN21AB5186.seq Check: 9985 from: 1 to: 5548
pFN21AB5186 5548 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3358 278
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2217 2891 1937
Size: 280 674 4594
Fragments arranged by size:
4594 674 280
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 3675 4048 1563
Size: 2112 373 3063
Fragments arranged by size:
3063 2112 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3611 4995 5263 1632
Size: 1979 1384 268 1917
Fragments arranged by size:
1979 1917 1384 268
AfeI AGC'GCT
Cuts at: 2513 2834 2513
Size: 321 5227
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 2238 4447 5424 1203
Size: 1035 2209 977 1327
Fragments arranged by size:
2209 1327 1035 977
AgeI A'CCGG_T
Cuts at: 4379 4379
Size: 5548
AhdI GACnn_n'nnGTC
Cuts at: 2377 4277 2377
Size: 1900 3648
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5548
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2549 2581 2549
Size: 32 5516
AlwNI CAG_nnn'CTG
Cuts at: 3291 4863 3291
Size: 1572 3976
ApaLI G'TGCA_C
Cuts at: 3605 4761 3605
Size: 1156 4392
ApoI r'AATT_y
Cuts at: 1493 2857 3083 3235 5344 1493
Size: 1364 226 152 2109 1697
Fragments arranged by size:
2109 1697 1364 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4102 160
Size: 872 3070 1606
Fragments arranged by size:
3070 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2719 2873 1870
Size: 73 95 681 154 4545
Fragments arranged by size:
4545 681 154 95 73
BamHI G'GATC_C
Cuts at: 2878 3245 2878
Size: 367 5181
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2271 2743 2869 4324 618
Size: 325 217 627 484 472 126 1455 1842
Fragments arranged by size:
1842 1455 627 484 472 325 217 126
BanII G_rGCy'C
Cuts at: 1391 2612 2867 1391
Size: 1221 255 4072
Fragments arranged by size:
4072 1221 255
BbeI G_GCGC'C
Cuts at: 2275 2275
Size: 5548
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4577
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3739 3773 1903
Size: 34 1802 34 3678
Fragments arranged by size:
3678 1802 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2069 3440 4650 2069
Size: 1371 1210 2967
Fragments arranged by size:
2967 1371 1210
BclI T'GATC_A
Cuts at: 1548 2022 1548
Size: 474 5074
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4159 136
Size: 107 122 71 631 605 2487 1525
Fragments arranged by size:
2487 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2529 5543 2529
Size: 3014 2534
BlpI GC'TnA_GC
Cuts at: 2965 2965
Size: 5548
Bme1580I G_kGCm'C
Cuts at: 1790 2039 2421 3609 4765 1790
Size: 249 382 1188 1156 2573
Fragments arranged by size:
2573 1188 1156 382 249
BmgBI CAC'GTC
Cuts at: 1318 2241 2416 2650 1318
Size: 923 175 234 4216
Fragments arranged by size:
4216 923 234 175
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3279 4237 442
Size: 416 53 83 399 24 1862 958 1753
Fragments arranged by size:
1862 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4208 1239
Size: 2969 2579
Bpu10I CC'TnA_GC
Cuts at: 2354 2354
Size: 5548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2372 3673 4538 4836 2372
Size: 1301 865 298 3084
Fragments arranged by size:
3084 1301 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2635 4211 882
Size: 617 1136 1576 2219
Fragments arranged by size:
2219 1576 1136 617
BsaAI yAC'GTr
Cuts at: 493 1204 3339 493
Size: 711 2135 2702
Fragments arranged by size:
2702 2135 711
BsaBI GATnn'nnATC
Cuts at: 5542 5542
Size: 5548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2157 2272 2727 3355
Size: 53 83 186 936 624 115 455 628
Cuts at: 3355 3737 4372 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 628 624 455 382 186
115 83 53
BsaWI w'CCGG_w
Cuts at: 3981 4379 4653 4800 5211 3981
Size: 398 274 147 411 4318
Fragments arranged by size:
4318 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 1178
Size: 30 165 30 912 30 4381
Fragments arranged by size:
4381 912 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 1186
Size: 1168 4380
BseYI C'CCAG_C
Cuts at: 2508 2597 2762 4751 5247 2508
Size: 89 165 1989 496 2809
Fragments arranged by size:
2809 1989 496 165 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2366 2603 2639 2657 1163
Size: 573 630 237 36 18 4054
Fragments arranged by size:
4054 630 573 237 36 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2146 2998 3759 4787 1540
Size: 275 186 145 852 761 1028 2301
Fragments arranged by size:
2301 1028 852 761 275 186 145
BsiHKAI G_wGCw'C
Cuts at: 3609 3694 4765 3609
Size: 85 1071 4392
Fragments arranged by size:
4392 1071 85
BsmI GAATG_Cn'
Cuts at: 3059 3152 3059
Size: 93 5455
BsmBI CGTCTCn'nnnn_
Cuts at: 2286 2286
Size: 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2421 2612 2867 3609 3694 4765
Size: 399 249 382 191 255 742 85 1071
Cuts at: 4765 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 382 255 249 191
85
BspEI T'CCGG_A
Cuts at: 5211 5211
Size: 5548
BspHI T'CATG_A
Cuts at: 3437 5167 3437
Size: 1730 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2625 2903 844
Size: 1781 278 3489
Fragments arranged by size:
3489 1781 278
BsrBI CCG'CTC
Cuts at: 1394 1823 3435 1394
Size: 429 1612 3507
Fragments arranged by size:
3507 1612 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4043 4217 65
Size: 1183 2795 174 1396
Fragments arranged by size:
2795 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2141 2693 2757 2807 4192 4379 1700
Size: 110 331 552 64 50 1385 187 2869
Fragments arranged by size:
2869 1385 552 331 187 110 64 50
BsrGI T'GTAC_A
Cuts at: 96 2784 96
Size: 2688 2860
BssHII G'CGCG_C
Cuts at: 1925 2571 2753 1925
Size: 646 182 4720
Fragments arranged by size:
4720 646 182
BssSI C'ACGA_G
Cuts at: 3608 4620 3608
Size: 1012 4536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5548
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 2444 1184
Size: 1260 4288
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2364 2529 2878 3245 3630 3647 5099 5185
Size: 392 165 349 367 385 17 1452 86
Cuts at: 5185 5197 5543 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 392 385 367 349 346 165
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2206 2583 513
Size: 552 1141 377 3478
Fragments arranged by size:
3478 1141 552 377
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2033 487
Size: 683 56 807 4002
Fragments arranged by size:
4002 807 683 56
BtsI GCAGTG_nn'
Cuts at: 3064 3858 3878 3064
Size: 794 20 4734
Fragments arranged by size:
4734 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5513
DraI TTT'AAA
Cuts at: 2852 2852
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2064 2118
Size: 54 1425 50 275 18 183 51 54
Cuts at: 2118 2143 2535 2586 2995 3884 5513 8
Size: 25 392 51 409 889 1629 43
Fragments arranged by size:
1629 1425 889 409 392 275 183 54
54 51 51 50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 2143 2995 1537
Size: 275 331 852 4090
Fragments arranged by size:
4090 852 331 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2582 3478 1353
Size: 1229 896 3423
Fragments arranged by size:
3423 1229 896
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4135 4509 4655 387
Size: 1064 138 2546 374 146 1280
Fragments arranged by size:
2546 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3611 4208 4995 5263 1239
Size: 393 1979 597 787 268 1524
Fragments arranged by size:
1979 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2857 3235 2857
Size: 378 5170
FspI TGC'GCA
Cuts at: 4054 4054
Size: 5548
HaeII r_GCGC'y
Cuts at: 1678 2275 2515 2669 2808 2836 4695 1678
Size: 597 240 154 139 28 1859 2531
Fragments arranged by size:
2531 1859 597 240 154 139 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 4189 4221 4263
Size: 32 135 32 791 32 1794 32 42
Cuts at: 4263 4295 1373
Size: 32 2626
Fragments arranged by size:
2626 1794 791 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2892 3138 669
Size: 988 281 954 246 3079
Fragments arranged by size:
3079 988 954 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5548
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 5548
KasI G'GCGC_C
Cuts at: 2271 2271
Size: 5548
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4662 4846 1305
Size: 3357 184 2007
Fragments arranged by size:
3357 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2588 10
Size: 54 1425 343 756 2970
Fragments arranged by size:
2970 1425 756 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2306 3506 3865 4024 518
Size: 648 1140 1200 359 159 2042
Fragments arranged by size:
2042 1200 1140 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2208 2350 2491 2700 2964 3641 4789 5034
Size: 602 142 141 209 264 677 1148 245
Cuts at: 5034 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 602 264 245 209 142
141
NaeI GCC'GGC
Cuts at: 2695 2759 2809 2695
Size: 64 50 5434
Fragments arranged by size:
5434 64 50
NarI GG'CG_CC
Cuts at: 2272 2272
Size: 5548
NcoI C'CATG_G
Cuts at: 513 1065 2583 513
Size: 552 1518 3478
Fragments arranged by size:
3478 1518 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5548
NgoMIV G'CCGG_C
Cuts at: 2693 2757 2807 2693
Size: 64 50 5434
Fragments arranged by size:
5434 64 50
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2995 2995
Size: 5548
NspI r_CATG'y
Cuts at: 1132 1229 2906 4451 1132
Size: 97 1677 1545 2229
Fragments arranged by size:
2229 1677 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5548
PciI A'CATG_T
Cuts at: 4447 4447
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5548
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 5130 5162 3617
Size: 32 1481 32 4003
Fragments arranged by size:
4003 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5548
PsiI TTA'TAA
Cuts at: 3118 3118
Size: 5548
PstI C_TGCA'G
Cuts at: 830 2900 830
Size: 2070 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5548
PvuII CAG'CTG
Cuts at: 2491 2491
Size: 5548
SacII CC_GC'GG
Cuts at: 2209 2209
Size: 5548
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SapI GCTCTTCn'nnn_
Cuts at: 2582 2582
Size: 5548
SbfI CC_TGCA'GG
Cuts at: 2900 2900
Size: 5548
ScaI AGT'ACT
Cuts at: 2642 3796 5508 2642
Size: 1154 1712 2682
Fragments arranged by size:
2682 1712 1154
SfcI C'TryA_G
Cuts at: 826 1045 2896 4031 4712 4903 826
Size: 219 1851 1135 681 191 1471
Fragments arranged by size:
1851 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2273 2273
Size: 5548
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5548
SmaI CCC'GGG
Cuts at: 1872 2721 2875 1872
Size: 849 154 4545
Fragments arranged by size:
4545 849 154
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2387 3652 4553 4815 820
Size: 197 926 444 1265 901 262 1553
Fragments arranged by size:
1553 1265 926 901 444 262 197
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2906 1132
Size: 1774 3774
SspI AAT'ATT
Cuts at: 5 52 3472 5
Size: 47 3420 2081
Fragments arranged by size:
3420 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5548
StyI C'CwwG_G
Cuts at: 513 1065 2103 2400 2583 2820 2987 513
Size: 552 1038 297 183 237 167 3074
Fragments arranged by size:
3074 1038 552 297 237 183 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3575 3592 3745 3930 4375 325
Size: 1255 1995 17 153 185 445 1498
Fragments arranged by size:
1995 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2640 2784 3794
Size: 274 80 33 51 1443 663 144 1010
Cuts at: 3794 5506 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1010 663 274 144 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5508 5540 5508
Size: 32 5516
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5548
XbaI T'CTAG_A
Cuts at: 2884 2884
Size: 5548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2407 2407
Size: 5548
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5548
XmaI C'CCGG_G
Cuts at: 1870 2719 2873 1870
Size: 849 154 4545
Fragments arranged by size:
4545 849 154
XmnI GAAnn'nnTTC
Cuts at: 3677 3677
Size: 5548
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3356 276
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI
SfiI SrfI SwaI