Restriction map for
pFN21AB5185
(Circular) (Six-base) MAPSORT of: pFN21AB5185.seq Check: 23 from: 1 to: 5899
pFN21AB5185 5899 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2839 2839
Size: 5899
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3709 278
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2640 3242 1937
Size: 703 602 4594
Fragments arranged by size:
4594 703 602
Acc65I G'GTAC_C
Cuts at: 2022 3220 2022
Size: 1198 4701
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2261 3158 3962 5346 5614 1632
Size: 629 897 804 1384 268 1917
Fragments arranged by size:
1917 1384 897 804 629 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5702
AflIII A'CryG_T
Cuts at: 1203 4798 5775 1203
Size: 3595 977 1327
Fragments arranged by size:
3595 1327 977
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 4628 4628
Size: 5899
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5899
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2336 3119 1184
Size: 1152 783 3964
Fragments arranged by size:
3964 1152 783
AlwNI CAG_nnn'CTG
Cuts at: 2596 2625 2728 2872 3642 5214 2596
Size: 29 103 144 770 1572 3281
Fragments arranged by size:
3281 1572 770 144 103 29
ApaI G_GGCC'C
Cuts at: 2703 3088 2703
Size: 385 5514
ApaLI G'TGCA_C
Cuts at: 3956 5112 3956
Size: 1156 4743
ApoI r'AATT_y
Cuts at: 1493 3208 3434 3586 5695 1493
Size: 1715 226 152 2109 1697
Fragments arranged by size:
2109 1715 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4453 160
Size: 872 3421 1606
Fragments arranged by size:
3421 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 2785 3224 1870
Size: 73 842 439 4545
Fragments arranged by size:
4545 842 439 73
BamHI G'GATC_C
Cuts at: 2289 3229 3596 2289
Size: 940 367 4592
Fragments arranged by size:
4592 940 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2022 2774 2846 2972 3031
Size: 325 217 627 235 752 72 126 59
Cuts at: 3031 3220 4675 618
Size: 189 1455 1842
Fragments arranged by size:
1842 1455 752 627 325 235 217 189
126 72 59
BanII G_rGCy'C
Cuts at: 1391 2087 2703 3088 3218 1391
Size: 696 616 385 130 4072
Fragments arranged by size:
4072 696 616 385 130
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4928
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4090 4124 1903
Size: 34 50 34 2069 34 3678
Fragments arranged by size:
3678 2069 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3791 5001 3791
Size: 1210 4689
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5899
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4510 136
Size: 107 122 71 631 605 2838 1525
Fragments arranged by size:
2838 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5894 5894
Size: 5899
BlpI GC'TnA_GC
Cuts at: 3316 3316
Size: 5899
Bme1580I G_kGCm'C
Cuts at: 1790 2253 2703 2779 3088 3960 5116 1790
Size: 463 450 76 309 872 1156 2573
Fragments arranged by size:
2573 1156 872 463 450 309 76
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5899
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3630 4588 442
Size: 416 53 83 399 24 2213 958 1753
Fragments arranged by size:
2213 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2621 2654 2657 2705 3194 4559 1239
Size: 1382 33 3 48 489 1365 2579
Fragments arranged by size:
2579 1382 1365 489 48 33 3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 2561 2823 4024 4889 5187 2525
Size: 36 262 1201 865 298 3237
Fragments arranged by size:
3237 1201 865 298 262 36
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4562 882
Size: 617 3063 2219
Fragments arranged by size:
3063 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3690 493
Size: 711 2486 2702
Fragments arranged by size:
2702 2486 711
BsaBI GATnn'nnATC
Cuts at: 5893 5893
Size: 5899
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3706 4088 4723 275
Size: 53 83 186 936 2173 382 635 1451
Fragments arranged by size:
2173 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4332 4730 5004 5151 5562 4332
Size: 398 274 147 411 4669
Fragments arranged by size:
4669 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2421 2451 1178
Size: 30 165 30 1018 30 4626
Fragments arranged by size:
4626 1018 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5899
BseYI C'CCAG_C
Cuts at: 2692 2696 2966 5102 5598 2692
Size: 4 270 2136 496 2993
Fragments arranged by size:
2993 2136 496 270 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2720 1163
Size: 573 984 4342
Fragments arranged by size:
4342 984 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3349 4110 5138 1540
Size: 275 186 1348 761 1028 2301
Fragments arranged by size:
2301 1348 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2673 3960 4045 5116 2673
Size: 1287 85 1071 3456
Fragments arranged by size:
3456 1287 1071 85
BsmI GAATG_Cn'
Cuts at: 3410 3503 3410
Size: 93 5806
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2253 2673 2703 2779 3088 3218
Size: 399 297 166 420 30 76 309 130
Cuts at: 3218 3960 4045 5116 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 420 399 309 297 166
130 85 76 30
BspEI T'CCGG_A
Cuts at: 5562 5562
Size: 5899
BspHI T'CATG_A
Cuts at: 3788 5518 3788
Size: 1730 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2004 2839 3087 3254 844
Size: 1160 835 248 167 3489
Fragments arranged by size:
3489 1160 835 248 167
BsrBI CCG'CTC
Cuts at: 1394 1823 2113 2263 3786 1394
Size: 429 290 150 1523 3507
Fragments arranged by size:
3507 1523 429 290 150
BsrDI GCAATG_nn'
Cuts at: 65 1248 4394 4568 65
Size: 1183 3146 174 1396
Fragments arranged by size:
3146 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4543 4730 1700
Size: 110 2733 187 2869
Fragments arranged by size:
2869 2733 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5899
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5899
BssSI C'ACGA_G
Cuts at: 2367 3959 4971 2367
Size: 1592 1012 3295
Fragments arranged by size:
3295 1592 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2774 794
Size: 1980 3919
BstBI TT'CG_AA
Cuts at: 4703 4703
Size: 5899
BstEII G'GTnAC_C
Cuts at: 1184 2209 1184
Size: 1025 4874
BstXI CCAn_nnnn'nTGG
Cuts at: 2309 3172 2309
Size: 863 5036
BstYI r'GATC_y
Cuts at: 1972 2289 2356 2559 3229 3596 3981 3998 5450
Size: 317 67 203 670 367 385 17 1452
Cuts at: 5450 5536 5548 5894 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 670 385 367 346 317 203
86 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 3185 3185
Size: 5899
BtgI C'CryG_G
Cuts at: 513 1065 2424 2802 513
Size: 552 1359 378 3610
Fragments arranged by size:
3610 1359 552 378
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5160
Fragments arranged by size:
5160 683 56
BtsI GCAGTG_nn'
Cuts at: 2664 3415 4209 4229 2664
Size: 751 794 20 4334
Fragments arranged by size:
4334 794 751 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5864
DraI TTT'AAA
Cuts at: 3203 3203
Size: 5899
DraIII CAC_nnn'GTG
Cuts at: 3031 3031
Size: 5899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4906 809
Size: 507 3590 1802
Fragments arranged by size:
3590 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3066 3162 3346
Size: 54 1425 50 275 18 1236 96 184
Cuts at: 3346 4235 5864 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1236 889 275 184 96 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3346 1537
Size: 275 1534 4090
Fragments arranged by size:
4090 1534 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3010 3829 1353
Size: 1657 819 3423
Fragments arranged by size:
3423 1657 819
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2267 2891 4486 4860 5006 387
Size: 1064 138 678 624 1595 374 146 1280
Fragments arranged by size:
1595 1280 1064 678 624 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2621 2654 2657 2705 3158 3194
Size: 393 629 360 33 3 48 453 36
Cuts at: 3194 3962 4559 5346 5614 1239
Size: 768 597 787 268 1524
Fragments arranged by size:
1524 787 768 629 597 453 393 360
268 48 36 33 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2347 2912 2347
Size: 565 5334
EcoO109I rG'GnC_Cy
Cuts at: 2306 2455 2533 2622 2917 2937 3085 3117 3189
Size: 149 78 89 295 20 148 32 72
Cuts at: 3189 2306
Size: 5016
Fragments arranged by size:
5016 295 149 148 89 78 72 32
20
EcoRI G'AATT_C
Cuts at: 3208 3586 3208
Size: 378 5521
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 5899
FspI TGC'GCA
Cuts at: 4405 4405
Size: 5899
HaeII r_GCGC'y
Cuts at: 1678 2033 2985 5046 1678
Size: 355 952 2061 2531
Fragments arranged by size:
2531 2061 952 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2341 2373 2421 2453 4540
Size: 32 135 32 769 32 48 32 2087
Cuts at: 4540 4572 4614 4646 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2087 769 135 48 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3243 3489 669
Size: 988 281 1305 246 3079
Fragments arranged by size:
3079 1305 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5899
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KpnI G_GTAC'C
Cuts at: 2026 3224 2026
Size: 1198 4701
MfeI C'AATT_G
Cuts at: 3498 3498
Size: 5899
MluI A'CGCG_T
Cuts at: 5775 5775
Size: 5899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5013 5197 1305
Size: 3708 184 2007
Fragments arranged by size:
3708 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3164 10
Size: 54 1425 343 1332 2745
Fragments arranged by size:
2745 1425 1332 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2504 3857 4216 4375 518
Size: 648 1338 1353 359 159 2042
Fragments arranged by size:
2042 1353 1338 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2551 2696 2728 2970 3315 3992 5140 5385
Size: 945 145 32 242 345 677 1148 245
Cuts at: 5385 1606
Size: 2120
Fragments arranged by size:
2120 1148 945 677 345 245 242 145
32
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5347
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5899
NotI GC'GGCC_GC
Cuts at: 3346 3346
Size: 5899
NspI r_CATG'y
Cuts at: 1132 1229 3257 4802 1132
Size: 97 2028 1545 2229
Fragments arranged by size:
2229 2028 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2038 2302 3112 1348
Size: 690 264 810 4135
Fragments arranged by size:
4135 810 690 264
PciI A'CATG_T
Cuts at: 4798 4798
Size: 5899
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2625 2728 1108
Size: 1517 103 4279
Fragments arranged by size:
4279 1517 103
PfoI T'CCnGG_A
Cuts at: 2991 2991
Size: 5899
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 5481 5513 3968
Size: 32 1481 32 4354
Fragments arranged by size:
4354 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2306 2455 2306
Size: 149 5750
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5899
PsiI TTA'TAA
Cuts at: 3469 3469
Size: 5899
PspOMI G'GGCC_C
Cuts at: 2699 3084 2699
Size: 385 5514
PstI C_TGCA'G
Cuts at: 830 3251 830
Size: 2421 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
PvuII CAG'CTG
Cuts at: 2696 2728 2696
Size: 32 5867
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SanDI GG'GwC_CC
Cuts at: 2455 2455
Size: 5899
SbfI CC_TGCA'GG
Cuts at: 3251 3251
Size: 5899
ScaI AGT'ACT
Cuts at: 4147 5859 4147
Size: 1712 4187
SfcI C'TryA_G
Cuts at: 826 1045 2897 3003 3247 4382 5063 5254 826
Size: 219 1852 106 244 1135 681 191 1471
Fragments arranged by size:
1852 1471 1135 681 244 219 191 106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5899
SmaI CCC'GGG
Cuts at: 1872 2787 3226 1872
Size: 915 439 4545
Fragments arranged by size:
4545 915 439
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2540 2576 2838 4003 4904 5166
Size: 197 926 597 36 262 1165 901 262
Cuts at: 5166 820
Size: 1553
Fragments arranged by size:
1553 1165 926 901 597 262 262 197
36
SnaBI TAC'GTA
Cuts at: 493 3690 493
Size: 3197 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 3257 1132
Size: 2125 3774
SspI AAT'ATT
Cuts at: 5 52 3823 5
Size: 47 3771 2081
Fragments arranged by size:
3771 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5899
StyI C'CwwG_G
Cuts at: 513 1065 2529 3338 513
Size: 552 1464 809 3074
Fragments arranged by size:
3074 1464 809 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2030 2990 3926 3943 4096 4281 4726
Size: 1255 450 960 936 17 153 185 445
Cuts at: 4726 325
Size: 1498
Fragments arranged by size:
1498 1255 960 936 450 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4145 5857 96
Size: 274 80 33 51 1443 2168 1712 138
Fragments arranged by size:
2168 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3015 3047 5859 5891 3015
Size: 32 2812 32 3023
Fragments arranged by size:
3023 2812 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5899
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5899
XmaI C'CCGG_G
Cuts at: 1870 2785 3224 1870
Size: 915 439 4545
Fragments arranged by size:
4545 915 439
XmnI GAAnn'nnTTC
Cuts at: 4028 4028
Size: 5899
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3707 276
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI XcmI